pGADT7-LOC434433(rice) plasmid

pGADT7-LOC434433(rice) plasmid

PVT96191

pGADT7-LOC434433(rice) plasmid Information

Plasmid name: pGADT7-LOC434433(rice)
Alias: XM_015791088.3
Gene lenght: 1782bp
Host: Yeast
Use(s): hybridization
Fragment type: ORF
Species: rice
Prokaryotic resistance: Amp
Screening marker: LEU2
Fluorescent labeling:
Promoter: ADH1
Replicon: 2U
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: GAL4AD
Reverse primer:
Copy number: Low
Induction:

pGADT7-LOC434433(rice) plasmid Description

pGADT7-LOC434433(rice) is a plasmid suitable for hybridization in yeast.

pGADT7-LOC434433(rice) plasmid Sequence

1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc 61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt 121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca 181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc 241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg 301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg 361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa 421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat 481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg 541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt 601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac 661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac 721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt 781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga 841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat 901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc 961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata 1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca 1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat 1141 gacaaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca 1201 gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc 1261 ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt 1321 gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta 1381 ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc 1441 acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa gatggataaa 1501 gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg taccgccgcc 1561 aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt cactaacagt 1621 agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc acaaccaatt 1681 gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag taaaattgat 1741 gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc gtataacgcg 1801 tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata taactatcta 1861 ttcgatgatg aagatacccc accaaaccca aaaaaagaga tctttaatac gactcactat 1921 agggcgagcg ccgccatgga gtacccatac gacgtaccag attacgctca tatgatggga 1981 gcagcggtga ccatggtgga tcggcgcatg gcgttcgcgg cggaggccga cgttgatagc 2041 aaggccgcct ttggattctt cggaggcgag tgcttcgtcg gcgagggcga cctggtcaac 2101 ccggcgccgc cgccgccgca gcagcagcag gtgcacgagg gcggattcgc cgccgaggac 2161 gagagcgacg gcgacgacga cgacgacgac gacgacgacg tggacgacat cgaggagctg 2221 gagcgccgca tgtggcgcga ccgcgtccgg cacaagcggc tcaaggagct tcagcagagc 2281 cgcgccggga gggagagccg ggcgggcgac gccggcggcg gaggcaggca gcagcggcag 2341 tcgcaggagc aggcgcggcg gaagaagatg tcgcgggcgc aggacgggat cctcaagtac 2401 atgctcaaga tgatggaggt gtgcaacgcg caggggttcg tgtacggcat catcccggag 2461 aaggggaagc cggtgagcgg cgcctccgac aacctccgct catggtggaa ggagaaggtc 2521 cgcttcgacc gcaatggccc ggccgccatc gccaagtacc aggccgacaa cgccgtgccg 2581 gggtgcgacg gcgacgccgg cggcgccgcg ccggcggggc cgcactccct gcacgagctg 2641 caggacacca cgctgggatc gctgctctcg gcgctcatgc agcactgcga cccgccgcag 2701 cgccgcttcc cgctggagaa gggcgtgccg ccgccgtggt ggccggaggg gagcgaggcg 2761 tggtggcccg aggccggcgt gcccaaggag ctcggcccgc cgccgtacaa gaagccgcac 2821 gacctcaaga aggcgtggaa ggtcgccgtg ctcaccgccg tcatcaagca catgtccccc 2881 gacgtcgaca aggtccgccg cctcgtgcgt cagtccaagt gcctccagga caagatgacc 2941 gccaaggaga tcgtcacctg gctcgccgtc ctcaagcagg aggaggacct ctatctcaag 3001 ctccatcccg gcgccctccc cccgccgctc tccgccgcgt ccttcaacgc cagcgtctcc 3061 ggcgagtacg acgtcgaagg agtcgacggc gacgaggccg ggaacaacaa cctgcagaag 3121 gcgcagaacg acgcgaccgc gttcatggac ctcaccacca ccatggatgc tgctctgagc 3181 aacaacaagt tcctgatcat gccgctgatg aaggaagagg ccatcgacgt cgacttcatc 3241 cagaagagga gcgagccgga gctgatgctg agcagcgaca gccatgcccg cgtctacacc 3301 tgcggcaacg tgcagtgccc gcacagcaac tacgcgctcg gcttcctcga ccgcaacgaa 3361 cgcaacgccc atcagtacgc ctgcaagcac aacgccgccg ccgccgccgc cgagagcaag 3421 ccgccgccgc cgcacatctt cgagccactc ggcagcttcg acttcgacct ccccgtcgac 3481 ggccagagat gcctcgccgg gctgatgacc atgtacgaca acgacgtcgc cgccgccacg 3541 cagatgcacc accaccacca tcagcagcag caggctaatt tcttcatccg cgacgacgcg 3601 ccgttcggag gcgacgtcgc cgccacggcg gcggcggcgc cggagttcag gttcagttca 3661 aatttcaacg tgacaggagg gggcgccgtg gactacggcg gcgccatgca gcaaccgccg 3721 gcgaagtacg ccggatccaa ctggttctac tgactcgagc tgcagatgaa tcgtagatac 3781 tgaaaaaccc cgcaagttca cttcaactgt gcatcgtgca ccatctcaat ttctttcatt 3841 tatacatcgt tttgccttct tttatgtaac tatactcctc taagtttcaa tcttggccat 3901 gtaacctctg atctatagaa ttttttaaat gactagaatt aatgcccatc ttttttttgg 3961 acctaaattc ttcatgaaaa tatattacga gggcttattc agaagctttg gacttcttcg 4021 ccagaggttt ggtcaagtct ccaatcaagg ttgtcggctt gtctaccttg ccagaaattt 4081 acgaaaagat ggaaaagggt caaatcgttg gtagatacgt tgttgacact tctaaataag 4141 cgaatttctt atgatttatg atttttatta ttaaataagt tataaaaaaa ataagtgtat 4201 acaaatttta aagtgactct taggttttaa aacgaaaatt cttattcttg agtaactctt 4261 tcctgtaggt caggttgctt tctcaggtat agcatgaggt cgctcttatt gaccacacct 4321 ctaccggccg gtcgaaattc ccctacccta tgaacatatt ccattttgta atttcgtgtc 4381 gtttctatta tgaatttcat ttataaagtt tatgtacaaa tatcataaaa aaagagaatc 4441 tttttaagca aggattttct taacttcttc ggcgacagca tcaccgactt cggtggtact 4501 gttggaacca cctaaatcac cagttctgat acctgcatcc aaaacctttt taactgcatc 4561 ttcaatggcc ttaccttctt caggcaagtt caatgacaat ttcaacatca ttgcagcaga 4621 caagatagtg gcgatagggt tgaccttatt ctttggcaaa tctggagcag aaccgtggca 4681 tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg acgcagatgg 4741 caacaaaccc aaggaacctg ggataacgga ggcttcatcg gagatgatat caccaaacat 4801 gttgctggtg attataatac catttaggtg ggttgggttc ttaactagga tcatggcggc 4861 agaatcaatc aattgatgtt gaaccttcaa tgtaggaaat tcgttcttga tggtttcctc 4921 cacagttttt ctccataatc ttgaagaggc caaaacatta gctttatcca aggaccaaat 4981 aggcaatggt ggctcatgtt gtagggccat gaaagcggcc attcttgtga ttctttgcac 5041 ttctggaacg gtgtattgtt cactatccca agcgacacca tcaccatcgt cttcctttct 5101 cttaccaaag taaatacctc ccactaattc tctgacaaca acgaagtcag tacctttagc 5161 aaattgtggc ttgattggag ataagtctaa aagagagtcg gatgcaaagt tacatggtct 5221 taagttggcg tacaattgaa gttctttacg gatttttagt aaaccttgtt caggtctaac 5281 actacctgta ccccatttag gaccacccac agcacctaac aaaacggcat caaccttctt 5341 ggaggcttcc agcgcctcat ctggaagtgg gacacctgta gcgtcgatag cagcaccacc 5401 aattaaatga ttttcgaaat cgaacttgac attggaacga acatcagaaa tagctttaag 5461 aaccttaatg gcttcggctg tgatttcttg accaacgtgg tcacctggca aaacgacgat 5521 cttcttaggg gcagacatta gaatggtata tccttgaaat atatatatat attgctgaaa 5581 tgtaaaaggt aagaaaagtt agaaagtaag acgattgcta accacctatt ggaaaaaaca 5641 ataggtcctt aaataatatt gtcaacttca agtattgtga tgcaagcatt tagtcatgaa 5701 cgcttctcta ttctatatga aaagccggtt ccggcgctct cacctttcct ttttctccca 5761 atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca aaaaatttcc 5821 agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat gttgaggaaa 5881 aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga gtattcccac 5941 agttggggat ctcgactcta gctagaggat caattcgtaa tcatggtcat agctgtttcc 6001 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 6061 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 6121 cgctttccag tcgggaaacc tgtcgtgcca gctgataact tcgtataatg tatgctatac 6181 gaagttatta ggtctgaaga ggagtttacg tccagccaag ctagcttggc tgcaggtcga 6241 gcggccgcga tccggaaccc ttaatataac ttcgtataat gtatgctata cgaagttatc 6301 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 6361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 6421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 6481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 6541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 6601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 6661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 6721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 6781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 6841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 6901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 6961 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 7021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 7081 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 7141 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 7201 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 7261 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 7321 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 7381 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 7441 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 7501 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 7561 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 7621 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 7681 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 7741 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 7801 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 7861 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 7921 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 7981 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 8041 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 8101 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 8161 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 8221 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 8281 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 8341 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 8401 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 8461 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 8521 agattgtact gagagtgcac cataacgcat ttaagcataa acacgcacta tgccgttctt 8581 ctcatgtata tatatataca ggcaacacgc agatataggt gcgacgtgaa cagtgagctg 8641 tatgtgcgca gctcgcgttg cattttcgga agcgctcgtt ttcggaaacg ctttgaagtt 8701 cctattccga agttcctatt ctctagctag aaagtatagg aacttcagag cgcttttgaa 8761 aaccaaaagc gctctgaaga cgcactttca aaaaaccaaa aacgcaccgg actgtaacga 8821 gctactaaaa tattgcgaat accgcttcca caaacattgc tcaaaagtat ctctttgcta 8881 tatatctctg tgctatatcc ctatataacc tacccatcca cctttcgctc cttgaacttg 8941 catctaaact cgacctctac attttttatg tttatctcta gtattactct ttagacaaaa 9001 aaattgtagt aagaactatt catagagtga atcgaaaaca atacgaaaat gtaaacattt 9061 cctatacgta gtatatagag acaaaataga agaaaccgtt cataattttc tgaccaatga 9121 agaatcatca acgctatcac tttctgttca caaagtatgc gcaatccaca tcggtataga 9181 atataatcgg ggatgccttt atcttgaaaa aatgcacccg cagcttcgct agtaatcagt 9241 aaacgcggga agtggagtca ggcttttttt atggaagaga aaatagacac caaagtagcc 9301 ttcttctaac cttaacggac ctacagtgca aaaagttatc aagagactgc attatagagc 9361 gcacaaagga gaaaaaaagt aatctaagat gctttgttag aaaaatagcg ctctcgggat 9421 gcatttttgt agaacaaaaa agaagtatag attctttgtt ggtaaaatag cgctctcgcg 9481 ttgcatttct gttctgtaaa aatgcagctc agattctttg tttgaaaaat tagcgctctc 9541 gcgttgcatt tttgttttac aaaaatgaag cacagattct tcgttggtaa aatagcgctt 9601 tcgcgttgca tttctgttct gtaaaaatgc agctcagatt ctttgtttga aaaattagcg 9661 ctctcgcgtt gcatttttgt tctacaaaat gaagcacaga tgcttcgttg ct

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.