pCMV-3×FLAG-MCF2L-Neo plasmid

pCMV-3×FLAG-MCF2L-Neo plasmid

PVT95092

pCMV-3×FLAG-MCF2L-Neo plasmid Information

Plasmid name: pCMV-3×FLAG-MCF2L(human)-Neo
Alias: NM_001112732.3;ARHGEF14; DBS; OST
Gene lenght: 3378bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×FLAG-MCF2L-Neo plasmid Description

pCMV-3×FLAG-MCF2L-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×FLAG-MCF2L-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaactt aagccaccat ggactacaaa gaccatgacg gtgattataa agatcatgac 961 atcgactaca aggatgacga tgacaagctt atgaggtttt ggctgaggac tgaagagatg 1021 gccttggaag aaatggtgca gagattaaat gcggtttcca agcacacgga tgaaatcatg 1081 caccaggaca tcgtcccgct ctgtgctgcc gacatccagg accagctaaa gaagcgcttt 1141 gcttacctgt ccggtgggcg ggggcaggac ggaagcccgg ttatcacctt ccctgactac 1201 ccggccttca gcgagattcc ggacaaggag ttccagaatg tcatgaccta cctcaccagc 1261 atccccagcc tgcaggacgc tggcatcgga ttcatcctgg tgatagaccg gcgacgggac 1321 aaatggacct ccgtgaaggc gtccgtcctg cgcatcgcag catctttccc ggcaaacctg 1381 cagctcgtcc tcgtgcttcg cccgacgggt tttttccaaa ggactctctc cgacatcgct 1441 ttcaaattca atagagatga ctttaagatg aaggtgccgg tcataatgct gagctccgta 1501 ccagacttac acggttacat cgataagtcg cagctgaccg aggacctggg tgggaccctg 1561 gactactgcc actcccggtg gctgtgccag cgcacggcca tcgaaagttt cgccctcatg 1621 gtgaagcaga cggctcagat gctgcagtcc ttcgggaccg agctggctga aacagagctg 1681 cccaatgacg tccagtcgac aagctcagtg ctgtgtgcgc acacagagaa gaaggacaag 1741 gcgaaggagg atttgaggct ggcactgaaa gaggggcaca gtgtcctgga gagcctcagg 1801 gagctgcagg ctgagggctc agagcccagt gtgaaccagg accagcttga caaccaggcc 1861 accgtgcaga ggctcctggc ccagctgaac gaaaccgagg ctgccttcga tgagttctgg 1921 gcaaagcatc agcagaaact ggagcagtgt ctgcagctcc ggcactttga gcagggcttc 1981 cgggaggtca aagccatctt ggacgcagcg tcccagaaga tagcaacctt cacagacatc 2041 ggcaacagcc tggcgcatgt ggagcacctg ctgagggacc tggccagctt cgaggagaaa 2101 tcaggcgtgg ccgtggagag ggcccgggcc ctgtctctgg acggcgagca gctcattggg 2161 aacaagcact acgcggtaga ctccatccgc ccaaagtgcc aggagctccg gcacctctgt 2221 gaccagttct ctgcggagat cgcaaggagg agggggctgc tcagcaagtc cctggagctg 2281 caccgccgcc tggagacgtc catgaagtgg tgtgatgaag ggatttacct gctggcctca 2341 caacctgtgg acaagtgcca gtcccaggac ggcgcggagg ctgccctcca ggaaatcgag 2401 aagtttttgg agaccggtgc ggaaaataag atccaggagc tcaacgcgat ttacaaggaa 2461 tacgaatcca tcctcaacca agatctcatg gagcacgtgc gaaaggtctt ccagaagcag 2521 gcaagcatgg aggaggtgtt ccaccgcagg caggccagcc tgaagaagct ggcggccagg 2581 cagacgcggc ccgtgcagcc ggtggccccc agacccgagg cactggcaaa gtcgccctgc 2641 ccctccccag gcattcggcg aggctctgag aactccagct ccgagggcgg tgcgctccgg 2701 agagggccct accggagggc caagagtgag atgagtgaga gccggcaggg ccgcggctca 2761 gcgggggagg aggaggaaag cctggccatc ctgcgcaggc acgtgatgag cgagctcctg 2821 gacacagaac gggcctacgt ggaggagctg ctgtgcgtcc tggagggcta cgccgcggag 2881 atggataacc cactgatggc tcacctcctg tcaacaggcc ttcacaacaa gaaggatgtt 2941 ttgtttggaa acatggagga aatctatcac ttccacaaca ggatattcct cagggagctg 3001 gaaaactaca ctgactgccc agaactggtt ggaagatgct ttctggagag gatggaagat 3061 ttccagatct atgagaagta ctgtcagaac aagccccgct ctgagagcct gtggagacag 3121 tgctccgact gcccgttttt ccaggaatgc cagagaaagc tggaccacaa gctgagcctg 3181 gactcctacc tgctgaagcc agtgcagagg atcaccaagt accagctgct gctcaaggaa 3241 atgctgaaat acagcaggaa ctgcgagggg gctgaggacc tgcaggaggc gctgagctcc 3301 atcctgggca tcctgaaggc cgtgaacgac tccatgcacc tcatcgctat caccggctat 3361 gacgggaatc tcggcgacct gggcaagctg ctgatgcagg gctcgttcag cgtctggacc 3421 gaccacaaga ggggccacac caaggtgaag gagctggcca ggttcaagcc catgcagcgg 3481 cacctgttcc tgcacgagaa ggcagtgctc ttctgcaaga agagggagga gaatggggag 3541 gggtatgaga aagctccctc ctacagctac aagcagtcct taaacatggc tgccgttggc 3601 attacggaga acgtgaaggg agatgctaag aagttcgaga tctggtacaa cgcgcgcgag 3661 gaggtctaca tcgtccaggc gccaactcct gagattaaag ccgcgtgggt gaatgaaatt 3721 cggaaagtgc tgaccagcca gctgcaggct tgtagagaag ccagccagca ccgggcgctg 3781 gagcagtcac agagcctgcc cctgccggcc ccgaccagca ccagtccctc aagaggaaac 3841 tcaaggaaca tcaagaagct ggaagaaagg aaaacagacc ccctaagcct ggagggatac 3901 gtcagctcag cgccactgac aaagcccccc gaaaagggca aaggttggag caaaacgtcc 3961 cactcactgg aggcacctga ggacgacggg ggctggtcaa gtgcagagga gcagattaac 4021 tcgtccgacg cagaggagga cggcgggttg ggccccaaga agctggttcc aggtaaatac 4081 acggtcgtgg cggaccacga gaagggaggc cccgatgcgc tgcgcgtgag gagcggggac 4141 gtggtggagc tggtgcagga gggcgacgag ggcctctggt acgtcaggga cccgaccact 4201 ggcaaggagg gctgggtgcc ggccagcagc ctgtccgtcc ggctcggccc gtccggctcg 4261 gcccagtgcc tgagcagctc agagtcgagc ccggggtcgg ccgtgctgag caactcgtcc 4321 agctgcagcg agggcggcca ggcccccttc tccgacctgc aggggtagga attctgcaga 4381 tatccagcac agtggcggcc gctcgagtct agagggcccg tttaaacccg ctgatcagcc 4441 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg 4501 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat 4561 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag 4621 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg 4681 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc 4741 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 4801 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 4861 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 4921 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 4981 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 5041 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat 5101 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt 5161 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 5221 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta 5281 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc 5341 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta 5401 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct 5461 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat 5521 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 5581 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 5641 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 5701 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc 5761 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 5821 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 5881 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 5941 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 6001 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 6061 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg 6121 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 6181 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 6241 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 6301 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg 6361 gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc 6421 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct 6481 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta 6541 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 6601 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc 6661 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 6721 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 6781 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 6841 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 6901 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 6961 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 7021 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 7081 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 7141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 7201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 7261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 7321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 7381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 7441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 7501 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 7561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 7621 tggtagcggt GGTttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 7681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 7741 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 7801 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 7861 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 7921 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 7981 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 8041 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 8101 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 8161 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 8221 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 8281 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 8341 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 8401 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 8461 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 8521 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 8581 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 8641 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 8701 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 8761 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 8821 atttccccga aaagtgccac ctgacgtc

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.