pLV3-CMV-FANCA-EGFP-EF1a-Puro

pLV3-CMV-FANCA-EGFP-EF1a-Puro plasmid

PVT96170

pLV3-CMV-FANCA-EGFP-EF1a-Puro plasmid Information

Plasmid name: pLV3-CMV-FANCA(human)-EGFP-EF1a-Puro
Alias: NM_000135.4;FA; FA-H; FA1; FAA; FACA; FAH; FANCH
Gene lenght: 4365bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-FANCA-EGFP-EF1a-Puro plasmid Description

pLV3-CMV-FANCA-EGFP-EF1a-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-FANCA-EGFP-EF1a-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc gccaccatgt ccgactcgtg ggtcccgaac tccgcctcgg gccaggaccc 2341 agggggccgc cggagggcct gggccgagct gctggcggga agggtcaaga gggaaaaata 2401 taatcctgaa agggcacaga aattaaagga atcagctgtg cgcctcctgc gaagccatca 2461 ggacctgaat gcccttttgc ttgaggtaga aggtccactg tgtaaaaaat tgtctctcag 2521 caaagtgatt gactgtgaca gttctgaggc ctatgctaat cattctagtt catttatagg 2581 ctctgctttg caggatcaag cctcaaggct gggggttccc gtgggtattc tctcagccgg 2641 gatggttgcc tctagcgtgg gacagatctg cacggctcca gcggagacca gtcaccctgt 2701 gctgctgact gtggagcaga gaaagaagct gtcttccctg ttagagtttg ctcagtattt 2761 attggcacac agtatgttct cccgtctttc cttctgtcaa gaattatgga aaatacagag 2821 ttctttgttg cttgaagcgg tgtggcatct tcacgtacaa ggcattgtga gcctgcaaga 2881 gctgctggaa agccatcccg acatgcatgc tgtgggatcg tggctcttca ggaatctgtg 2941 ctgcctttgt gaacagatgg aagcatcctg ccagcatgct gacgtcgcca gggccatgct 3001 ttctgatttt gttcaaatgt ttgttttgag gggatttcag aaaaactcag atctgagaag 3061 aactgtggag cctgaaaaaa tgccgcaggt cacggttgat gtactgcaga gaatgctgat 3121 ttttgcactt gacgctttgg ctgctggagt acaggaggag tcctccactc acaagatcgt 3181 gaggtgctgg ttcggagtgt tcagtggaca cacgcttggc agtgtaattt ccacagatcc 3241 tctgaagagg ttcttcagtc ataccctgac tcagatactc actcacagcc ctgtgctgaa 3301 agcatctgat gctgttcaga tgcagagaga gtggagcttt gcgcggacac accctctgct 3361 cacctcactg taccgcaggc tctttgtgat gctgagtgca gaggagttgg ttggccattt 3421 gcaagaagtt ctggaaacgc aggaggttca ctggcagaga gtgctctcct ttgtgtctgc 3481 cctggttgtc tgctttccag aagcgcagca gctgcttgaa gactgggtgg cgcgtttgat 3541 ggcccaggca ttcgagagct gccagctgga cagcatggtc actgcgttcc tggttgtgcg 3601 ccaggcagca ctggagggcc cctctgcgtt cctgtcatat gcagactggt tcaaggcctc 3661 ctttgggagc acacgaggct accatggctg cagcaagaag gccctggtct tcctgtttac 3721 gttcttgtca gaactcgtgc cttttgagtc tccccggtac ctgcaggtgc acattctcca 3781 cccacccctg gttcccggca agtaccgctc cctcctcaca gactacatct cattggccaa 3841 gacacggctg gccgacctca aggtttctat agaaaacatg ggactctacg aggatttgtc 3901 atcagctggg gacattactg agccccacag ccaagctctt caggatgttg aaaaggccat 3961 catggtgttt gagcatacgg ggaacatccc agtcaccgtc atggaggcca gcatattcag 4021 gaggccttac tacgtgtccc acttcctccc cgccctgctc acacctcgag tgctccccaa 4081 agtccctgac tcccgtgtgg cgtttataga gtctctgaag agagcagata aaatcccccc 4141 atctctgtac tccacctact gccaggcctg ctctgctgct gaagagaagc cagaagatgc 4201 agccctggga gtgagggcag aacccaactc tgctgaggag cccctgggac agctcacagc 4261 tgcactggga gagctgagag cctccatgac agaccccagc cagcgtgatg ttatatcggc 4321 acaggtggca gtgatttctg aaagactgag ggctgtcctg ggccacaatg aggatgacag 4381 cagcgttgag atatcaaaga ttcagctcag catcaacacg ccgagactgg agccacggga 4441 acacatggct gtggacctcc tgctgacgtc tttctgtcag aacctgatgg ctgcctccag 4501 tgtcgctccc ccggagaggc agggtccctg ggctgccctc ttcgtgagga ccatgtgtgg 4561 acgtgtgctc cctgcagtgc tcacccggct ctgccagctg ctccgtcacc agggcccgag 4621 cctgagtgcc ccacatgtgc tggggttggc tgccctggcc gtgcacctgg gtgagtccag 4681 gtctgcgctc ccagaggtgg atgtgggtcc tcctgcacct ggtgctggcc ttcctgtccc 4741 tgcgctcttt gacagcctcc tgacctgtag gacgagggat tccttgttct tctgcctgaa 4801 attttgtaca gcagcaattt cttactctct ctgcaagttt tcttcccagt cacgagatac 4861 tttgtgcagc tgcttatctc caggccttat taaaaagttt cagttcctca tgttcagatt 4921 gttctcagag gcccgacagc ctctttctga ggaggacgta gccagccttt cctggagacc 4981 cttgcacctt ccttctgcag actggcagag agctgccctc tctctctgga cacacagaac 5041 cttccgagag gtgttgaaag aggaagatgt tcacttaact taccaagact ggttacacct 5101 ggagctggaa attcaacctg aagctgatgc tctttcagat actgaacggc aggacttcca 5161 ccagtgggcg atccatgagc actttctccc tgagtcctcg gcttcagggg gctgtgacgg 5221 agacctgcag gctgcgtgta ccattcttgt caacgcactg atggatttcc accaaagctc 5281 aaggagttat gaccactcag aaaattctga tttggtcttt ggtggccgca caggaaatga 5341 ggatattatt tccagattgc aggagatggt agctgacctg gagctgcagc aagacctcat 5401 agtgcctctc ggccacaccc cttcccagga gcacttcctc tttgagattt tccgcagacg 5461 gctccaggct ctgacaagcg ggtggagcgt ggctgccagc cttcagagac agagggagct 5521 gctaatgtac aaacggatcc tcctccgcct gccttcgtct gtcctctgcg gcagcagctt 5581 ccaggcagaa cagcccatca ctgccagatg cgagcagttc ttccacttgg tcaactctga 5641 gatgagaaac ttctgctccc acggaggtgc cctgacacag gacatcactg cccacttctt 5701 caggggcctc ctgaacgcct gtctgcggag cagagacccc tccctgatgg tcgacttcat 5761 actggccaag tgccagacga aatgcccctt aattttgacc tctgctctgg tgtggtggcc 5821 gagcctggag cctgtgctgc tctgccggtg gaggagacac tgccagagcc cgctgccccg 5881 ggaactgcag aagctacaag aaggccggca gtttgccagc gatttcctct cccctgaggc 5941 tgcctcccca gcacccaacc cggactggct ctcagctgct gcactgcact ttgcgattca 6001 acaagtcagg gaagaaaaca tcaggaagca gctaaagaag ctggactgcg agagagagga 6061 gctattggtt ttccttttct tcttctcctt gatgggcctg ctgtcgtcac atctgacctc 6121 aaatagcacc acagacctgc caaaggcttt ccacgtttgt gcagcaatcc tcgagtgttt 6181 agagaagagg aagatatcct ggctggcact ctttcagttg acagagagtg acctcaggct 6241 ggggcggctc ctcctccgtg tggccccgga tcagcacacc aggctgctgc ctttcgcttt 6301 ttacagtctt ctctcctact tccatgaaga cgcggccatc agggaagagg ccttcctgca 6361 tgttgctgtg gacatgtact tgaagctggt ccagctcttc gtggctgggg atacaagcac 6421 agtttcacct ccagctggca ggagcctgga gctcaagggt cagggcaacc ccgtggaact 6481 gataacaaaa gctcgtcttt ttctgctgca gttaatacct cggtgcccga aaaagagctt 6541 ctcacacgtg gcagagctgc tggctgatcg tggggactgc gacccagagg tgagcgccgc 6601 cctccagagc agacagcagg ctgcccctga cgctgacctg tcccaggagc ctcatctctt 6661 cggatccGGA GGAGGGGGAA GCatggtgag caagggcgag gagctgttca ccggggtggt 6721 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 6781 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 6841 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 6901 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 6961 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 7021 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 7081 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 7141 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 7201 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 7261 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 7321 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 7381 catggacgag ctgtacaagt aagcggccgc gaaggatctg cgatcgctcc ggtgcccgtc 7441 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt 7501 gaacgggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc 7561 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg 7621 ttctttttcg caacgggttt gccgccagaa cacagctgaa gcttcgaggg gctcgcatct 7681 ctccttcacg cgcccgccgc cctacctgag gccgccatcc acgccggttg agtcgcgttc 7741 tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc 7801 tcaggtcgag accgggcctt tgtccggcgc tcccttggag cctacctaga ctcagccggc 7861 tctccacgct ttgcctgacc ctgcttgctc aactctacgt ctttgtttcg ttttctgttc 7921 tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct agatgaccga gtacaagccc 7981 acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg 8041 ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc 8101 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 8161 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 8221 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 8281 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 8341 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 8401 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 8461 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 8521 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gagtcgacaa tcaacctctg 8581 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 8641 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 8701 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 8761 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 8821 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 8881 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 8941 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc 9001 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 9061 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 9121 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gtacctttaa gaccaatgac 9181 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct 9241 aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc tctggttaga 9301 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 9361 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 9421 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 9481 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt 9541 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 9601 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 9661 tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 9721 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 9781 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg 9841 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 9901 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 9961 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 10021 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc 10081 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca 10141 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca 10201 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 10261 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 10321 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 10381 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 10441 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 10501 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 10561 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 10621 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 10681 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 10741 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 10801 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 10861 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta 10921 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa 10981 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc 11041 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact 11101 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc 11161 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt 11221 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta 11281 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg 11341 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt 11401 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc 11461 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt 11521 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc 11581 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc 11641 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa 11701 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac 11761 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa 11821 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt 11881 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 11941 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 12001 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg 12061 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg 12121 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg 12181 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta 12241 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 12301 atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc 12361 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta 12421 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.