pLV3-CMV-FANCA-3×FLAG-Puro pla

pLV3-CMV-FANCA-3×FLAG-Puro plasmid

PVT94566

pLV3-CMV-FANCA-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-FANCA(human)-3×FLAG-Puro
Alias: NM_000135.4;FA; FA-H; FA1; FAA; FACA; FAH; FANCH
Gene lenght: 4365bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-FANCA-3×FLAG-Puro plasmid Description

pLV3-CMV-FANCA-3×FLAG-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-FANCA-3×FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catgtccgac tcgtgggtcc cgaactccgc ctcgggccag 2341 gacccagggg gccgccggag ggcctgggcc gagctgctgg cgggaagggt caagagggaa 2401 aaatataatc ctgaaagggc acagaaatta aaggaatcag ctgtgcgcct cctgcgaagc 2461 catcaggacc tgaatgccct tttgcttgag gtagaaggtc cactgtgtaa aaaattgtct 2521 ctcagcaaag tgattgactg tgacagttct gaggcctatg ctaatcattc tagttcattt 2581 ataggctctg ctttgcagga tcaagcctca aggctggggg ttcccgtggg tattctctca 2641 gccgggatgg ttgcctctag cgtgggacag atctgcacgg ctccagcgga gaccagtcac 2701 cctgtgctgc tgactgtgga gcagagaaag aagctgtctt ccctgttaga gtttgctcag 2761 tatttattgg cacacagtat gttctcccgt ctttccttct gtcaagaatt atggaaaata 2821 cagagttctt tgttgcttga agcggtgtgg catcttcacg tacaaggcat tgtgagcctg 2881 caagagctgc tggaaagcca tcccgacatg catgctgtgg gatcgtggct cttcaggaat 2941 ctgtgctgcc tttgtgaaca gatggaagca tcctgccagc atgctgacgt cgccagggcc 3001 atgctttctg attttgttca aatgtttgtt ttgaggggat ttcagaaaaa ctcagatctg 3061 agaagaactg tggagcctga aaaaatgccg caggtcacgg ttgatgtact gcagagaatg 3121 ctgatttttg cacttgacgc tttggctgct ggagtacagg aggagtcctc cactcacaag 3181 atcgtgaggt gctggttcgg agtgttcagt ggacacacgc ttggcagtgt aatttccaca 3241 gatcctctga agaggttctt cagtcatacc ctgactcaga tactcactca cagccctgtg 3301 ctgaaagcat ctgatgctgt tcagatgcag agagagtgga gctttgcgcg gacacaccct 3361 ctgctcacct cactgtaccg caggctcttt gtgatgctga gtgcagagga gttggttggc 3421 catttgcaag aagttctgga aacgcaggag gttcactggc agagagtgct ctcctttgtg 3481 tctgccctgg ttgtctgctt tccagaagcg cagcagctgc ttgaagactg ggtggcgcgt 3541 ttgatggccc aggcattcga gagctgccag ctggacagca tggtcactgc gttcctggtt 3601 gtgcgccagg cagcactgga gggcccctct gcgttcctgt catatgcaga ctggttcaag 3661 gcctcctttg ggagcacacg aggctaccat ggctgcagca agaaggccct ggtcttcctg 3721 tttacgttct tgtcagaact cgtgcctttt gagtctcccc ggtacctgca ggtgcacatt 3781 ctccacccac ccctggttcc cggcaagtac cgctccctcc tcacagacta catctcattg 3841 gccaagacac ggctggccga cctcaaggtt tctatagaaa acatgggact ctacgaggat 3901 ttgtcatcag ctggggacat tactgagccc cacagccaag ctcttcagga tgttgaaaag 3961 gccatcatgg tgtttgagca tacggggaac atcccagtca ccgtcatgga ggccagcata 4021 ttcaggaggc cttactacgt gtcccacttc ctccccgccc tgctcacacc tcgagtgctc 4081 cccaaagtcc ctgactcccg tgtggcgttt atagagtctc tgaagagagc agataaaatc 4141 cccccatctc tgtactccac ctactgccag gcctgctctg ctgctgaaga gaagccagaa 4201 gatgcagccc tgggagtgag ggcagaaccc aactctgctg aggagcccct gggacagctc 4261 acagctgcac tgggagagct gagagcctcc atgacagacc ccagccagcg tgatgttata 4321 tcggcacagg tggcagtgat ttctgaaaga ctgagggctg tcctgggcca caatgaggat 4381 gacagcagcg ttgagatatc aaagattcag ctcagcatca acacgccgag actggagcca 4441 cgggaacaca tggctgtgga cctcctgctg acgtctttct gtcagaacct gatggctgcc 4501 tccagtgtcg ctcccccgga gaggcagggt ccctgggctg ccctcttcgt gaggaccatg 4561 tgtggacgtg tgctccctgc agtgctcacc cggctctgcc agctgctccg tcaccagggc 4621 ccgagcctga gtgccccaca tgtgctgggg ttggctgccc tggccgtgca cctgggtgag 4681 tccaggtctg cgctcccaga ggtggatgtg ggtcctcctg cacctggtgc tggccttcct 4741 gtccctgcgc tctttgacag cctcctgacc tgtaggacga gggattcctt gttcttctgc 4801 ctgaaatttt gtacagcagc aatttcttac tctctctgca agttttcttc ccagtcacga 4861 gatactttgt gcagctgctt atctccaggc cttattaaaa agtttcagtt cctcatgttc 4921 agattgttct cagaggcccg acagcctctt tctgaggagg acgtagccag cctttcctgg 4981 agacccttgc accttccttc tgcagactgg cagagagctg ccctctctct ctggacacac 5041 agaaccttcc gagaggtgtt gaaagaggaa gatgttcact taacttacca agactggtta 5101 cacctggagc tggaaattca acctgaagct gatgctcttt cagatactga acggcaggac 5161 ttccaccagt gggcgatcca tgagcacttt ctccctgagt cctcggcttc agggggctgt 5221 gacggagacc tgcaggctgc gtgtaccatt cttgtcaacg cactgatgga tttccaccaa 5281 agctcaagga gttatgacca ctcagaaaat tctgatttgg tctttggtgg ccgcacagga 5341 aatgaggata ttatttccag attgcaggag atggtagctg acctggagct gcagcaagac 5401 ctcatagtgc ctctcggcca caccccttcc caggagcact tcctctttga gattttccgc 5461 agacggctcc aggctctgac aagcgggtgg agcgtggctg ccagccttca gagacagagg 5521 gagctgctaa tgtacaaacg gatcctcctc cgcctgcctt cgtctgtcct ctgcggcagc 5581 agcttccagg cagaacagcc catcactgcc agatgcgagc agttcttcca cttggtcaac 5641 tctgagatga gaaacttctg ctcccacgga ggtgccctga cacaggacat cactgcccac 5701 ttcttcaggg gcctcctgaa cgcctgtctg cggagcagag acccctccct gatggtcgac 5761 ttcatactgg ccaagtgcca gacgaaatgc cccttaattt tgacctctgc tctggtgtgg 5821 tggccgagcc tggagcctgt gctgctctgc cggtggagga gacactgcca gagcccgctg 5881 ccccgggaac tgcagaagct acaagaaggc cggcagtttg ccagcgattt cctctcccct 5941 gaggctgcct ccccagcacc caacccggac tggctctcag ctgctgcact gcactttgcg 6001 attcaacaag tcagggaaga aaacatcagg aagcagctaa agaagctgga ctgcgagaga 6061 gaggagctat tggttttcct tttcttcttc tccttgatgg gcctgctgtc gtcacatctg 6121 acctcaaata gcaccacaga cctgccaaag gctttccacg tttgtgcagc aatcctcgag 6181 tgtttagaga agaggaagat atcctggctg gcactctttc agttgacaga gagtgacctc 6241 aggctggggc ggctcctcct ccgtgtggcc ccggatcagc acaccaggct gctgcctttc 6301 gctttttaca gtcttctctc ctacttccat gaagacgcgg ccatcaggga agaggccttc 6361 ctgcatgttg ctgtggacat gtacttgaag ctggtccagc tcttcgtggc tggggataca 6421 agcacagttt cacctccagc tggcaggagc ctggagctca agggtcaggg caaccccgtg 6481 gaactgataa caaaagctcg tctttttctg ctgcagttaa tacctcggtg cccgaaaaag 6541 agcttctcac acgtggcaga gctgctggct gatcgtgggg actgcgaccc agaggtgagc 6601 gccgccctcc agagcagaca gcaggctgcc cctgacgctg acctgtccca ggagcctcat 6661 ctcttcGGAT CCGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC 6721 AAGGATGACG ATGACAAGTA GCGGCCGCGA AGGATCTGCG ATCGCTCCGG TGCCCGTCAG 6781 TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT CGGCAATTGA 6841 ACGGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT GTACTGGCTC 6901 CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC CGTGAACGTT 6961 CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGCTGAAGC TTCGAGGGGC TCGCATCTCT 7021 CCTTCACGCG CCCGCCGCCC TACCTGAGGC CGCCATCCAC GCCGGTTGAG TCGCGTTCTG 7081 CCGCCTCCCG CCTGTGGTGC CTCCTGAACT GCGTCCGCCG TCTAGGTAAG TTTAAAGCTC 7141 AGGTCGAGAC CGGGCCTTTG TCCGGCGCTC CCTTGGAGCC TACCTAGACT CAGCCGGCTC 7201 TCCACGCTTT GCCTGACCCT GCTTGCTCAA CTCTACGTCT TTGTTTCGTT TTCTGTTCTG 7261 CGCCGTTACA GATCCAAGCT GTGACCGGCG CCTACGCTAG ATGACCGAGT ACAAGCCCAC 7321 GGTGCGCCTC GCCACCCGCG ACGACGTCCC CCGGGCCGTA CGCACCCTCG CCGCCGCGTT 7381 CGCCGACTAC CCCGCCACGC GCCACACCGT CGACCCGGAC CGCCACATCG AGCGGGTCAC 7441 CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC 7501 GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT 7561 GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA 7621 ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC 7681 CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG 7741 AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA 7801 CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG 7861 ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA GTCGACAATC AACCTCTGGA 7921 TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG 7981 TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT 8041 CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG 8101 GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC 8161 CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA 8221 ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA 8281 TTCCGTGGTG TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC 8341 CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT 8401 TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA 8461 GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCCTGGT ACCTTTAAGA CCAATGACTT 8521 ACAAGGCAGC TGTAGATCTT AGCCACTTTT TAAAAGAAAA GGGGGGACTG GAAGGGCTAA 8581 TTCACTCCCA ACGAAAATAA GATCTGCTTT TTGCTTGTAC TGGGTCTCTC TGGTTAGACC 8641 AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC ACTGCTTAAG CCTCAATAAA 8701 GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT GTGTGACTCT GGTAACTAGA 8761 GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG CAGTAGTAGT TCATGTCATC 8821 TTATTATTCA GTATTTATAA CTTGCAAAGA AATGAATATC AGAGAGTGAG AGGAACTTGT 8881 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 8941 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 9001 TCTGGCTCTA GCTATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG 9061 CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA 9121 GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGACTTTTGC AGAGACGGCC CAAATTCGTA 9181 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT 9241 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT 9301 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA 9361 ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT GGGCGCTCTT CCGCTTCCTC 9421 GCTCACTGAC TCGCTGCGCT CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA 9481 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 9541 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 9601 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 9661 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 9721 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 9781 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 9841 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 9901 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 9961 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 10021 CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 10081 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 10141 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 10201 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 10261 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 10321 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC 10381 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC 10441 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC 10501 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG 10561 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG 10621 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC 10681 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC 10741 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG 10801 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC 10861 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG 10921 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC 10981 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT 11041 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG 11101 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA 11161 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT 11221 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG 11281 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA 11341 CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC 11401 CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC AGCTCCCGGA 11461 GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC AGGGCGCGTC 11521 AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC AGATTGTACT 11581 GAGAGTGCAC CATATGCGGT GTGAAATACC GCACAGATGC GTAAGGAGAA AATACCGCAT 11641 CAGGCGCCAT TCGCCATTCA GGCTGCGCAA CTGTTGGGAA GGGCGATCGG TGCGGGCCTC 11701 TTCGCTATTA CGCCAGCTGG CGAAAGGGGG ATGTGCTGCA AGGCGATTAA GTTGGGTAAC 11761 GCCAGGGTTT TCCCAGTCAC GACGTTGTAA AACGACGGCC AGTGCCAAGC TG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.