pCAG-HA-PTGS1 plasmid

pCAG-HA-PTGS1 plasmid

PVT95783

pCAG-HA-PTGS1 plasmid Information

Plasmid name: pCAG-HA-PTGS1(human)
Alias: NM_000962.4：COX1; COX3; PCOX1; PES-1; PGG/HS; PGHS-1;PGHS1; PHS1; PTGHS
Gene lenght: 1800bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CAG
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pcaggs-F
Reverse primer:
Copy number: High
Induction:

pCAG-HA-PTGS1 plasmid Description

pCAG-HA-PTGS1 is a plasmid suitable for protein expression in mammalian cells.

pCAG-HA-PTGS1 plasmid Sequence

1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA 61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC 121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG 181 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC 241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG 301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG 361 TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA 421 TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG 481 CGATGGGGGC GGGGGGGGGG GGGGGGCCCC CCCCAGGCGG GGCGGGGCGG GGCGAGGGGC 541 GGGGCGGGGC GAGGCGGAAA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT 601 TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG 661 CGGGAGTCGT TGCGCGCTGC CTTCCCCCCG TGCCCCGCTC CGCCGCCGCC TCGCGCCGCC 721 CGCCCCGGCT CTGACTGACC GCGTTACTCC CACAGGTGAG CGGGCGGGAC GGCCCTTCTC 781 CTCCGGGCTG TAATTAGCGC TTGGTTTAAT GACGGCTTGT TTCTTTTCTG TGGCTGCGTG 841 AAAGCCTTGA GGGGCTCCGG GAGGGCCCTT TGTGCGGGGG GAGCGGCTCG GGGGGTGCGT 901 GCGTGTGTGT GTGCGTGGGG AGCGCCGCGT GCGGCTCCGC GCTGCCCGGC GGCTGTGAGC 961 GCTGCGGGCG CGGCGCGGGG CTTTGTGCGC TCCGCAGTGT GCGCGAGGGG AGCGCGGCCG 1021 GGGGCGGTGC CCCGCGGTGC GGGGGGGGCT GCGAGGGGAA CAAAGGCTGC GTGCGGGGTG 1081 TGTGCGTGGG GGGGTGAGCA GGGGGTGTGG GCGCGTCGGT CGGGCTGCAA CCCCCCCTGC 1141 ACCCCCCTCC CCGAGTTGCT GAGCACGGCC CGGCTTCGGG TGCGGGGCTC CGTACGGGGC 1201 GTGGCGCGGG GCTCGCCGTG CCGGGCGGGG GGTGGCGGCA GGTGGGGGTG CCGGGCGGGG 1261 CGGGGCCGCC TCGGGCCGGG GAGGGCTCGG GGGAAGGGGC GCGGCGGCCC CCGGAGCGCC 1321 GGCGGCTGTC GAGGCGCGGC GAGCCGCAGC CATTGCCTTT TATGGTAATC GTGCGAGAGG 1381 GCGCAGGGAC TTCCTTTGTC CCAAATCTGT GCGGAGCCGA AATCTGGGAG GCGCCGCCGC 1441 ACCCCCTCTA GCGGGCGCGG GGCGAAGCGG TGCGGCGCCG GCAGGAAGGA AATGGGCGGG 1501 GAGGGCCTTC GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC CTCTCCAGCC TCGGGGCTGT 1561 CCGCGGGGGG ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT 1621 GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG 1681 CTCCTGGGCA ACGTGCTGGT TGTTGTGCTG TCTCATCATT TTGGCAAAGC CACCATGTAC 1741 CCATACGATG TTCCAGATTA CGCTGAATTC atgagccgga gtctcttgct ctggttcttg 1801 ctgttcctgc tcctgctccc gccgctcccc gtcctgctcg cggacccagg ggcgcccacg 1861 ccagtgaatc cctgttgtta ctatccatgc cagcaccagg gcatctgtgt ccgcttcggc 1921 cttgaccgct accagtgtga ctgcacccgc acgggctatt ccggccccaa ctgcaccatc 1981 cctggcctgt ggacctggct ccggaattca ctgcggccca gcccctcttt cacccacttc 2041 ctgctcactc acgggcgctg gttctgggag tttgtcaatg ccaccttcat ccgagagatg 2101 ctcatgcgcc tggtactcac agtgcgctcc aaccttatcc ccagtccccc cacctacaac 2161 tcagcacatg actacatcag ctgggagtct ttctccaacg tgagctatta cactcgtatt 2221 ctgccctctg tgcctaaaga ttgccccaca cccatgggaa ccaaagggaa gaagcagttg 2281 ccagatgccc agctcctggc ccgccgcttc ctgctcagga ggaagttcat acctgacccc 2341 caaggcacca acctcatgtt tgccttcttt gcacaacact tcacccacca gttcttcaaa 2401 acttctggca agatgggtcc tggcttcacc aaggccttgg gccatggggt agacctcggc 2461 cacatttatg gagacaatct ggagcgtcag tatcaactgc ggctctttaa ggatgggaaa 2521 ctcaagtacc aggtgctgga tggagaaatg tacccgccct cggtagaaga ggcgcctgtg 2581 ttgatgcact acccccgagg catcccgccc cagagccaga tggctgtggg ccaggaggtg 2641 tttgggctgc ttcctgggct catgctgtat gccacgctct ggctacgtga gcacaaccgt 2701 gtgtgtgacc tgctgaaggc tgagcacccc acctggggcg atgagcagct tttccagacg 2761 acccgcctca tcctcatagg ggagaccatc aagattgtca tcgaggagta cgtgcagcag 2821 ctgagtggct atttcctgca gctgaaattt gacccagagc tgctgttcgg tgtccagttc 2881 caataccgca accgcattgc catggagttc aaccatctct accactggca ccccctcatg 2941 cctgactcct tcaaggtggg ctcccaggag tacagctacg agcagttctt gttcaacacc 3001 tccatgttgg tggactatgg ggttgaggcc ctggtggatg ccttctctcg ccagattgct 3061 ggccggatcg gtgggggcag gaacatggac caccacatcc tgcatgtggc tgtggatgtc 3121 atcagggagt ctcgggagat gcggctgcag cccttcaatg agtaccgcaa gaggtttggc 3181 atgaaaccct acacctcctt ccaggagctc gtaggagaga aggagatggc agcagagttg 3241 gaggaattgt atggagacat tgatgcgttg gagttctacc ctggactgct tcttgaaaag 3301 tgccatccaa actctatctt tggggagagt atgatagaga ttggggctcc cttttccctc 3361 aagggtctcc tagggaatcc catctgttct ccggagtact ggaagccgag cacatttggc 3421 ggcgaggtgg gctttaacat tgtcaagacg gccacactga agaagctggt ctgcctcaac 3481 accaagacct gtccctacgt ttccttccgt gtgccggatg ccagtcagga tgatgggcct 3541 gctgtggagc gaccatccac agagctctga CTCGAGCTAG CAGATCTTAA TTAATTAAGG 3601 ATCTTTTTCC CTCTGCCAAA AATTATGGGG ACATCATGAA GCCCCTTGAG CATCTGACTT 3661 CTGGCTAATA AAGGAAATTT ATTTTCATTG CAATAGTGTG TTGGAATTTT TTGTGTCTCT 3721 CACTCGGAAG GACATATGGG AGGGCAAATC ATTTAAAACA TCAGAATGAG TATTTGGTTT 3781 AGAGTTTGGC AACATATGCC CATATGCTGG CTGCCATGAA CAAAGGTTGG CTATAAAGAG 3841 GTCATCAGTA TATGAAACAG CCCCCTGCTG TCCATTCCTT ATTCCATAGA AAAGCCTTGA 3901 CTTGAGGTTA GATTTTTTTT ATATTTTGTT TTGTGTTATT TTTTTCTTTA ACATCCCTAA 3961 AATTTTCCTT ACATGTTTTA CTAGCCAGAT TTTTCCTCCT CTCCTGACTA CTCCCAGTCA 4021 TAGCTGTCCC TCTTCTCTTA TGGAGATCCC TCGACCTGCA GCCCAAGCTT GGCGTAATCA 4081 TGGTCATAGC TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA 4141 GCCGGAAGCA TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT 4201 GCGTTGCGCT CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCG GATCCGCATC 4261 TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC 4321 CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG 4381 AGGCCGCCTC GGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG 4441 GCTTTTGCAA AAAGCTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG 4501 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA 4561 ACTCATCAAT GTATCTTATC ATGTCTGGAT CCGCTGCATT AATGAATCGG CCAACGCGCG 4621 GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC 4681 TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC 4741 ACAGAATCAG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA 4801 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC 4861 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG 4921 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT 4981 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT 5041 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC 5101 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG 5161 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG 5221 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG 5281 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG 5341 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA 5401 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA 5461 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA 5521 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT 5581 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT 5641 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT 5701 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG 5761 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT 5821 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT 5881 TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG 5941 CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA 6001 AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT 6061 TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT 6121 GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC 6181 CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC GCCACATAGC AGAACTTTAA 6241 AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT 6301 TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT 6361 TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA 6421 GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT 6481 ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA 6541 TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.