pLV3-CMV-Bnip3-CopGFP-Puro pla

pLV3-CMV-Bnip3-CopGFP-Puro plasmid

PVT94832

pLV3-CMV-Bnip3-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Bnip3(mouse)-CopGFP-Puro
Alias: NM_009760.4;N; Nip3
Gene lenght: 564bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Bnip3-CopGFP-Puro plasmid Description

pLV3-CMV-Bnip3-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Bnip3-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgt cgcagagcgg ggaggagaac 2341 ctgcagggct cctgggtaga actgcacttc agcaatggca atgggagcag cgttccagcc 2401 tccgtctcta tttataatgg tgacatggaa aaaatactgt tggatgccca gcatgaatct 2461 ggacgaagta gctccaagag ttctcactgt gacagcccac ctcgctccca gacaccacaa 2521 gataccaaca gagctgaaat agacagccac agctttggcg agaaaaacag cactctgtct 2581 gaggaagatt atattgagag aagaagagaa gttgaaagta tcctgaagaa aaactcagat 2641 tggatatggg attggtcaag tcgaccagaa aatattcccc ccaaggagtt cctttttaaa 2701 cacccgaagc gcacagctac tctcagcatg agaaacacaa gcgttatgaa gaaaggggga 2761 attttctcag cagactttct gaaggttttc cttccatctc tgttactgtc tcatctgctg 2821 gccattggct tggggatcta cattggaagg cgtctgacaa cttccactag caccttctga 2881 ggatccCTGC GATATCCAGC gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 2941 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 3001 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 3061 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 3121 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 3181 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 3241 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 3301 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 3361 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 3421 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 3481 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 3541 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 3601 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 3661 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 3721 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 3781 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 3841 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 3901 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 3961 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 4021 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 4081 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 4141 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 4201 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 4261 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 4321 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 4381 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 4441 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 4501 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 4561 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 4621 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 4681 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 4741 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 4801 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 4861 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 4921 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 4981 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 5041 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 5101 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 5161 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 5221 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg 5281 tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 5341 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 5401 cggcctcttc cgcgtctccg ccttcgccct cagacgagtc ggatctccct ttggccgcct 5461 ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt 5521 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaaata agatctgctt 5581 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa 5641 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt 5701 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg 5761 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag 5821 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa 5881 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 5941 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc 6001 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 6061 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 6121 tagacttttg cagagacggc ccaaattcgt aatcatggtc atagctgttt cctgtgtgaa 6181 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 6241 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 6301 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 6361 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 6421 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 6481 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 6541 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 6601 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 6661 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 6721 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 6781 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 6841 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 6901 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 6961 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 7021 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 7081 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 7141 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 7201 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 7261 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 7321 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 7381 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 7441 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 7501 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 7561 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 7621 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 7681 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 7741 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 7801 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 7861 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 7921 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 7981 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 8041 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 8101 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 8161 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 8221 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 8281 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 8341 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg 8401 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 8461 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc 8521 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 8581 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca 8641 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg 8701 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta 8761 aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.