pCAG-SFPQ-3×FLAG plasmid

pCAG-SFPQ-3×FLAG plasmid

PVT94813

pCAG-SFPQ-3×FLAG plasmid Information

Plasmid name: pCAG-SFPQ(human)-3×FLAG
Alias: NM_005066.3;POMP100; PPP1R140; PSF
Gene lenght: 2121bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: CAG
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pcaggs-F
Reverse primer:
Copy number: High
Induction:

pCAG-SFPQ-3×FLAG plasmid Description

pCAG-SFPQ-3×FLAG is a plasmid suitable for protein expression in mammalian cells.

pCAG-SFPQ-3×FLAG plasmid Sequence

1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA 61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC 121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG 181 GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC 241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG 301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG 361 TATTAGTCAT CGCTATTACC ATGGTCGAGG TGAGCCCCAC GTTCTGCTTC ACTCTCCCCA 421 TCTCCCCCCC CTCCCCACCC CCAATTTTGT ATTTATTTAT TTTTTAATTA TTTTGTGCAG 481 CGATGGGGGC GGGGGGGGGG GGGGGGCCCC CCCCAGGCGG GGCGGGGCGG GGCGAGGGGC 541 GGGGCGGGGC GAGGCGGAAA GGTGCGGCGG CAGCCAATCA GAGCGGCGCG CTCCGAAAGT 601 TTCCTTTTAT GGCGAGGCGG CGGCGGCGGC GGCCCTATAA AAAGCGAAGC GCGCGGCGGG 661 CGGGAGTCGT TGCGCGCTGC CTTCCCCCCG TGCCCCGCTC CGCCGCCGCC TCGCGCCGCC 721 CGCCCCGGCT CTGACTGACC GCGTTACTCC CACAGGTGAG CGGGCGGGAC GGCCCTTCTC 781 CTCCGGGCTG TAATTAGCGC TTGGTTTAAT GACGGCTTGT TTCTTTTCTG TGGCTGCGTG 841 AAAGCCTTGA GGGGCTCCGG GAGGGCCCTT TGTGCGGGGG GAGCGGCTCG GGGGGTGCGT 901 GCGTGTGTGT GTGCGTGGGG AGCGCCGCGT GCGGCTCCGC GCTGCCCGGC GGCTGTGAGC 961 GCTGCGGGCG CGGCGCGGGG CTTTGTGCGC TCCGCAGTGT GCGCGAGGGG AGCGCGGCCG 1021 GGGGCGGTGC CCCGCGGTGC GGGGGGGGCT GCGAGGGGAA CAAAGGCTGC GTGCGGGGTG 1081 TGTGCGTGGG GGGGTGAGCA GGGGGTGTGG GCGCGTCGGT CGGGCTGCAA CCCCCCCTGC 1141 ACCCCCCTCC CCGAGTTGCT GAGCACGGCC CGGCTTCGGG TGCGGGGCTC CGTACGGGGC 1201 GTGGCGCGGG GCTCGCCGTG CCGGGCGGGG GGTGGCGGCA GGTGGGGGTG CCGGGCGGGG 1261 CGGGGCCGCC TCGGGCCGGG GAGGGCTCGG GGGAAGGGGC GCGGCGGCCC CCGGAGCGCC 1321 GGCGGCTGTC GAGGCGCGGC GAGCCGCAGC CATTGCCTTT TATGGTAATC GTGCGAGAGG 1381 GCGCAGGGAC TTCCTTTGTC CCAAATCTGT GCGGAGCCGA AATCTGGGAG GCGCCGCCGC 1441 ACCCCCTCTA GCGGGCGCGG GGCGAAGCGG TGCGGCGCCG GCAGGAAGGA AATGGGCGGG 1501 GAGGGCCTTC GTGCGTCGCC GCGCCGCCGT CCCCTTCTCC CTCTCCAGCC TCGGGGCTGT 1561 CCGCGGGGGG ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT 1621 GTGACCGGCG GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG 1681 CTCCTGGGCA ACGTGCTGGT TATTGTGCTG TCTCATCATT TTGGCAAAGA ATTCGCCACC 1741 atgtctcggg atcggttccg gagtcgtggc ggtggcggtg gtggcttcca caggcgtgga 1801 ggaggcggcg gccgcggcgg cctccacgac ttccgttctc cgccgcccgg catgggcctc 1861 aatcagaatc gcggccccat gggtcctggc ccgggccaga gcggccctaa gcctccgatc 1921 ccgccaccgc ctccacacca acagcagcaa cagccaccac cgcagcagcc accgccgcag 1981 cagccgccac cgcatcagcc gccgccgcat ccacagccgc atcagcagca gcagccgccg 2041 ccaccgccgc aggactcttc caagcccgtc gttgctcagg gacccggccc cgctcccgga 2101 gtaggcagcg caccaccagc ctccagctcg gccccgcccg ccactccacc aacctcgggg 2161 gccccgccag ggtccgggcc aggcccgact ccgaccccgc cgcctgcagt cacctcggcc 2221 cctcccgggg cgccgccacc caccccgcca agcagcgggg tccctaccac acctcctcag 2281 gccggaggcc cgccgcctcc gcccgcggca gtcccgggcc cgggtccagg gcctaagcag 2341 ggcccaggtc cgggtggtcc caaaggcggc aaaatgcctg gcgggccgaa gccaggtggc 2401 ggcccgggcc taagtacgcc tggcggccac cccaagccgc cgcatcgagg cggcggggag 2461 ccccgcgggg gccgccagca ccacccgccc taccaccagc agcatcacca ggggcccccg 2521 cccggcgggc ccggcggccg cagcgaggag aagatctcgg actcggaggg gtttaaagcc 2581 aatttgtctc tcttgaggag gcctggagag aaaacttaca cacagcgatg tcggttgttt 2641 gttgggaatc tacctgctga tatcacggag gatgaattca aaagactatt tgctaaatat 2701 ggagaaccag gagaagtttt tatcaacaaa ggcaaaggat tcggatttat taagcttgaa 2761 tctagagctt tggctgaaat tgccaaagcc gaactggatg atacacccat gagaggtaga 2821 cagcttcgag ttcgctttgc cacacatgct gctgcccttt ctgttcgtaa tctttcacct 2881 tatgtttcca atgaactgtt ggaagaagcc tttagccaat ttggtcctat tgaaagggct 2941 gttgtaatag tggatgatcg tggaagatct acagggaaag gcattgttga atttgcttct 3001 aagccagcag caagaaaggc atttgaacga tgcagtgaag gtgttttctt actgacgaca 3061 actcctcgtc cagtcattgt ggaaccactt gaacaactag atgatgaaga tggtcttcct 3121 gaaaaacttg cccagaagaa tccaatgtat caaaaggaga gagaaacccc tcctcgtttt 3181 gcccagcatg gcacgtttga gtacgaatat tctcagcgat ggaagtcttt ggatgaaatg 3241 gaaaaacagc aaagggaaca agttgaaaaa aacatgaaag atgcaaaaga caaattggaa 3301 agtgaaatgg aagatgccta tcatgaacat caggcaaatc ttttgcgcca agatctgatg 3361 agacgacagg aagaattaag acgcatggaa gaacttcaca atcaagaaat gcagaaacgt 3421 aaagaaatgc aattgaggca agaggaggaa cgacgtagaa gagaggaaga gatgatgatt 3481 cgtcaacgtg agatggaaga acaaatgagg cgccaaagag aggaaagtta cagccgaatg 3541 ggctacatgg atccacggga aagagacatg cgaatgggtg gcggaggagc aatgaacatg 3601 ggagatccct atggttcagg aggccagaaa tttccacctc taggaggtgg tggtggcata 3661 ggttatgaag ctaatcctgg cgttccacca gcaaccatga gtggttccat gatgggaagt 3721 gacatgcgta ctgagcgctt tgggcaggga ggtgcggggc ctgtgggtgg acagggtcct 3781 agaggaatgg ggcctggaac tccagcagga tatggtagag ggagagaaga gtacgaaggc 3841 ccaaacaaaa aaccccgatt tCTCGAGGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 3901 CATGACATCG ACTACAAGGA TGACGATGAC AAGTAGCTAG CAGATCTTTT TCCCTCTGCC 3961 AAAAATTATG GGGACATCAT GAAGCCCCTT GAGCATCTGA CTTCTGGCTA ATAAAGGAAA 4021 TTTATTTTCA TTGCAATAGT GTGTTGGAAT TTTTTGTGTC TCTCACTCGG AAGGACATAT 4081 GGGAGGGCAA ATCATTTAAA ACATCAGAAT GAGTATTTGG TTTAGAGTTT GGCAACATAT 4141 GCCCATATGC TGGCTGCCAT GAACAAAGGT TGGCTATAAA GAGGTCATCA GTATATGAAA 4201 CAGCCCCCTG CTGTCCATTC CTTATTCCAT AGAAAAGCCT TGACTTGAGG TTAGATTTTT 4261 TTTATATTTT GTTTTGTGTT ATTTTTTTCT TTAACATCCC TAAAATTTTC CTTACATGTT 4321 TTACTAGCCA GATTTTTCCT CCTCTCCTGA CTACTCCCAG TCATAGCTGT CCCTCTTCTC 4381 TTATGGAGAT CCCTCGACCT GCAGCCCAAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC 4441 TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG 4501 TAAAGCCTGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC 4561 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CGGATCCGCA TCTCAATTAG TCAGCAACCA 4621 TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC 4681 CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG 4741 AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTAA 4801 CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA 4861 TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA 4921 TCATGTCTGG ATCCGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT 4981 ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG 5041 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGATAACG 5101 CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT 5161 TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA 5221 GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT 5281 CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC 5341 CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG 5401 TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT 5461 TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG 5521 CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA 5581 AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA 5641 AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG 5701 GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG 5761 AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG 5821 GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT 5881 GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG GTCTGACAGT TACCAATGCT 5941 TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA GTTGCCTGAC 6001 TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC AGTGCTGCAA 6061 TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC AGCAATAAAC CAGCCAGCCG 6121 GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG TCTATTAATT 6181 GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC GTTGTTGCCA 6241 TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC AGCTCCGGTT 6301 CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG GTTAGCTCCT 6361 TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC ATGGTTATGG 6421 CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT GTGACTGGTG 6481 AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC TCTTGCCCGG 6541 CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC ATCATTGGAA 6601 AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC AGTTCGATGT 6661 AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC GTTTCTGGGT 6721 GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA CGGAAATGTT 6781 GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA 6841 TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT 6901 TTCCCCGAAA AGTGCCACCT GG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.