pCMV-TRAPPC9-3×HA-Neo plasmid

pCMV-TRAPPC9-3×HA-Neo plasmid

PVT95566

pCMV-TRAPPC9-3×HA-Neo plasmid Information

Plasmid name: pCMV-TRAPPC9(human)-3×HA-Neo
Alias: NM_031466.8;IBP; IKBKBBP; MRT13; NIBP; T1; TRS120
Gene lenght: 3447bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-TRAPPC9-3×HA-Neo plasmid Description

pCMV-TRAPPC9-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-TRAPPC9-3×HA-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CACTAGTCCA GTGTGGTGGA ATTCgccacc atgagcgtcc ctgactacat gcagtgtgct 961 gaggaccacc agacgctgct cgtggtggtc cagcctgtgg gcatcgtctc cgaggagaac 1021 ttcttcagga tctataagag gatttgctct gtgagtcaga tcagcgtgcg ggactcccag 1081 cgagtcctct acatccgcta caggcaccac tacccacccg agaacaacga gtggggtgac 1141 ttccagaccc accgcaaagt cgtgggcctc atcaccatca cagactgctt ctcggccaag 1201 gactggccac agacctttga gaagttccac gtgcagaagg agatctacgg ctccacactg 1261 tatgactccc ggctctttgt cttcgggctg cagggggaga tcgtggagca gccgcgcacc 1321 gacgtggctt tctaccccaa ctacgaggac tgccagacgg tggagaagag aatcgaggac 1381 ttcatcgagt cactgttcat cgtgctggag tccaagcgtc tggacagagc cacagacaag 1441 tctggggata agatccccct tctctgtgtc ccgtttgaga aaaaggactt tgtaggactg 1501 gacacagaca gcagacatta caagaagcgg tgccaaggcc gcatgcggaa gcacgtgggg 1561 gacctgtgcc tgcaggcagg gatgctgcag gactccctgg tgcattacca catgtcggtg 1621 gagctgctgc gttctgtgaa tgactttctg tggcttggag ctgccctgga aggattgtgt 1681 tcagcttctg tcatctatca ctatcctggt ggaactggtg ggaagagtgg agctcggagg 1741 ttccagggca gcacccttcc tgctgaagca gccaatagac accggccagg ggcacaggaa 1801 gttctcattg atccaggtgc cctcaccacc aatggcatca accctgacac cagtactgag 1861 atcggacgtg ctaagaactg ccttagccct gaagacataa ttgacaagta taaagaggcg 1921 atttcctatt acagcaagta taagaatgcg ggagtgattg agttggaagc gtgcatcaag 1981 gctgtacgtg tccttgcaat tcagaaacgg agcatggaag catcagaatt tcttcagaat 2041 gcagtttaca ttaaccttcg acagctttct gaggaagaga aaattcagcg ctacagcatc 2101 ctctccgagc tctatgagct gatcggcttc catcgcaagt ctgcgttctt caagcgcgtg 2161 gccgccatgc agtgcgtggc cccaagcatc gcggagcctg ggtggagggc ctgctacaaa 2221 ctcctcctgg aaacgctgcc cggctacagt ctgtcgctgg atcccaaaga tttcagcaga 2281 ggcacgcaca gaggctgggc tgcggtccag atgcgtttgc tccatgaatt ggtctacgcc 2341 tcccgaagga tggggaaccc tgccctctct gtcagacacc tgtccttcct tctacagacc 2401 atgctggact tcttgtcgga tcaggaaaag aaagatgtgg cccaaagcct agagaactat 2461 acgtccaagt gtcctgggac catggagccc atcgccctcc ctggcggcct caccctgcca 2521 ccggtgccct tcaccaagct tcccatcgtc aggcatgtga aactattgaa ccttcctgct 2581 agcctccggc cacacaaaat gaaaagcttg ctgggtcaga acgtgtcaac caaaagtcct 2641 ttcatctatt caccaattat cgcacacaac cgtggagaag agcggaacaa gaaaatagat 2701 ttccagtggg ttcaaggaga tgtgtgtgaa gttcagctga tggtatataa cccaatgccg 2761 tttgaacttc gagttgaaaa catggggctg ctcaccagcg gagtggagtt cgagtctctc 2821 cctgcggcgc tttctcttcc ggctgaatct ggtctgtacc cagtgacgct cgtcggggtc 2881 ccgcagacga ctggaacgat tactgtgaac ggttaccata ccacggtctt cggtgtgttc 2941 agtgactgtt tgctggataa cctgccggga ataaaaacca gtggctccac agtggaagtc 3001 attcccgcgt tgccaagact gcagatcagc acctctctgc ccagatctgc acattcattg 3061 caaccttctt ctggtgatga aatatctact aatgtatctg tccagcttta caatggagaa 3121 agtcagcaac taatcattaa attggaaaat attggaatgg aaccattgga gaaactggag 3181 gtcacctcga aagttctcac cactaaagaa aaattgtatg gcgacttctt gagctggaag 3241 ctagaggaaa cccttgccca gttccctttg cagcctggga aggtggccac gttcacaatc 3301 aacatcaaag tgaagctgga tttctcctgc caggagaatc tcctgcagga tctcagtgat 3361 gatggaatca gtgtgagtgg ctttcccctg tccagtcctt ttcggcaggt cgttcggccc 3421 cgagtggagg gcaaacctgt gaacccaccc gagagcaaca aagcaggcga ctacagccac 3481 gtgaagaccc tggaagctgt cctgaatttc aaatactctg gaggcccggg ccacactgaa 3541 ggatattaca ggaatctctc cctggggctg catgtagaag tcgagccgtc tgtatttttc 3601 acccgagtca gcaccctccc agcaaccagt acccggcagt gtcacctgct cctggatgtc 3661 ttcaactcca ccgagcatga gctgaccgtc agcaccagga gcagcgaggc actcatcctg 3721 cacgccggtg agtgccagcg aatggctatt caagtggaca agttcaactt tgagagtttc 3781 ccggagtccc ctggggagaa ggggcaattt gcaaacccca agcagctgga ggaagagcgg 3841 cgggaagccc gaggcctgga gatccacagc aagctgggca tctgctggag aatcccctcc 3901 ctgaagcgca gtggcgaggc gagtgtggaa ggactcctga accagctcgt cctggagcac 3961 ctgcagctgg cgcctctgca gtgggatgtg ctggtggacg gacagccatg tgaccgcgag 4021 gctgtggcgg cctgccaggt gggcgacccc gtgcgcctgg aggtgcggct gaccaaccgg 4081 agcccgcgca gcgtagggcc cttcgccctc actgtggtcc ccttccagga ccaccagaac 4141 ggcgtgcaca actacgacct gcacgacacc gtctccttcg tgggctccag caccttctac 4201 ctcgacgcgg tgcagccgtc cggccagtcg gcctgcctcg gggccctcct cttcctctac 4261 acgggagact tcttcctcca catccggttc cacgaggaca gcaccagcaa ggagctgcca 4321 ccctcttggt tctgcctgcc cagtgtgcac gtgtgtgccc tggaggcgca ggccCTCGAG 4381 TACCCATACG ACGTACCTGA TTATGCAACT TATCCTTATG ATGTACCTGA TTATGCTAGC 4441 TATCCTTATG ATGTCCCAGA CTACGCAtga GGGCCCGTTT AAACCCGCTG ATCAGCCTCG 4501 ACTGTGCCTT CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC 4561 CTGGAAGGTG CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT 4621 CTGAGTAGGT GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT 4681 TGGGAAGACA ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA 4741 AGAACCAGCT GGGGCTCTAG GGGGTATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG 4801 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 4861 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 4921 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 4981 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 5041 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 5101 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 5161 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT AATTCTGTGG AATGTGTGTC 5221 AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC 5281 TCAATTAGTC AGCAACCAGG TGTGGAAAGT CCCCAGGCTC CCCAGCAGGC AGAAGTATGC 5341 AAAGCATGCA TCTCAATTAG TCAGCAACCA TAGTCCCGCC CCTAACTCCG CCCATCCCGC 5401 CCCTAACTCC GCCCAGTTCC GCCCATTCTC CGCCCCATGG CTGACTAATT TTTTTTATTT 5461 ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG AGCTATTCCA GAAGTAGTGA GGAGGCTTTT 5521 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG 5581 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT 5641 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT 5701 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA 5761 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG 5821 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT 5881 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG 5941 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT 6001 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG 6061 GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT 6121 TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG 6181 CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC 6241 GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG 6301 AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT 6361 CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT 6421 CGAAATGACC GACCAAGCGA CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC 6481 CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTCCGGGAC GCCGGCTGGA TGATCCTCCA 6541 GCGCGGGGAT CTCATGCTGG AGTTCTTCGC CCACCCCAAC TTGTTTATTG CAGCTTATAA 6601 TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA 6661 TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGTA TACCGTCGAC 6721 CTCTAGCTAG AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC 6781 GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA 6841 ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA 6901 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT 6961 TGGGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG 7021 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 7081 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 7141 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 7201 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 7261 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 7321 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 7381 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 7441 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 7501 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 7561 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 7621 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 7681 TAGCGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 7741 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 7801 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 7861 TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT 7921 CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC 7981 CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT 8041 ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG 8101 GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG 8161 CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC 8221 TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA 8281 ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG 8341 TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC 8401 ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA 8461 CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC 8521 AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA TTGGAAAACG 8581 TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT CGATGTAACC 8641 CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT CTGGGTGAGC 8701 AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA AATGTTGAAT 8761 ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG 8821 CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC 8881 CCGAAAAGTG CCACCTGACG TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.