pT3-EF1a-RBM22 plasmid

pT3-EF1a-RBM22 plasmid

PVT94708

pT3-EF1a-RBM22 plasmid Information

Plasmid name: pT3-EF1a-RBM22(human)
Alias: NM_018047.3;Cwc2; fSAP47; ZC3H16
Gene lenght: 1263bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7
Reverse primer:
Copy number: High
Induction:

pT3-EF1a-RBM22 plasmid Description

pT3-EF1a-RBM22 is a plasmid suitable for protein expression in mammalian cells.

pT3-EF1a-RBM22 plasmid Sequence

1 GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG 61 GGAGGGGTCG GCAATTGAAC CGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT 121 GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA 181 GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GGTAAGTGCC 241 GTGTGTGGTT CCCGCGGGCC TGGCCTCTTT ACGGGTTATG GCCCTTGCGT GCCTTGAATT 301 ACTTCCACCT GGCTGCAGTA CGTGATTCTT GATCCCGAGC TTCGGGTTGG AAGTGGGTGG 361 GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG 421 CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT 481 TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG 541 CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC 601 GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA 661 GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT 721 GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA GGCTGGCCCG GTCGGCACCA 781 GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTG CAGGGAGCTC AAAATGGAGG 841 ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCACAC AAAGGAAAAG GGCCTTTCCG 901 TCCTCAGCCG TCGCTTCATG TGACTCCACG GAGTACCGGG CGCCGTCCAG GCACCTCGAT 961 TAGTTCTCGA GCTTTTGGAG TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG 1021 GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA 1081 TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA 1141 GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GAATTAGCTT GGTACTAATA 1201 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGGTAAGCTT GGTACCGAGC TCGGATCCAC 1261 TAGTCCAGTG TGGTGGAATT CTGCAGATAT CAACAAGTTT GTACAAAAAA GCAGGCTCCG 1321 CGGCCGCCCC CTTCAGCCAC Catggcgacc tctctgggtt ccaacaccta caacaggcag 1381 aactgggagg atgcggactt ccccattctg tgccagacat gtcttggaga aaacccatat 1441 atccgaatga ccaaagaaaa gtatgggaag gaatgcaaaa tctgtgccag gccattcaca 1501 gtgtttcgct ggtgccctgg agtccgcatg cgtttcaaga agactgaagt gtgccaaacc 1561 tgcagtaaat tgaagaatgt ctgtcagacc tgcctcttag acctagagta tggcctgccc 1621 atccaggttc gtgacgcagg attgtctttt aaagatgaca tgccaaagtc agatgtcaac 1681 aaagagtact atacacagaa tatggagaga gagatttcta actctgatgg aacacggcca 1741 gttggcatgc tggggaaagc cacatctacc agtgacatgc tgctcaaact ggcccggacc 1801 acaccctact acaaaaggaa tcgaccccac atttgctcct tctgggtgaa aggagagtgt 1861 aagagaggag aggaatgtcc atacagacat gagaagccta cagatccaga tgaccccctt 1921 gctgatcaga atattaaaga ccgttattac ggaatcaatg atcctgtagc tgacaagctt 1981 ctaaagcggg cttcaacaat gcctcggctg gacccaccag aggataaaac tatcaccaca 2041 ctatatgttg gtggtctagg tgataccatt actgagacag atttaagaaa tcatttctac 2101 cagttcggag agatccggac gatcactgtt gtgcagagac agcagtgtgc tttcatccag 2161 tttgccacac ggcaggctgc agaagtggct gctgagaagt cctttaataa gttgattgta 2221 aatggccgca gactgaatgt gaaatgggga agatcccagg cagccagagg aaaagaaaaa 2281 gagaaagatg gaactacaga ctctgggatc aaactagaac ctgttccagg attgccagga 2341 gctcttcctc ctcctcctgc agcagaagaa gaagcctctg ccaactactt caacttgccc 2401 ccaagtggtc ctccagctgt ggtgaacatt gctctgccac cgccccctgg cattgctcca 2461 cccccacccc caggttttgg gccacacatg ttccacccaa tgggaccacc ccctcctttc 2521 atgcgggctc caggaccaat ccactatcct tctcaggacc ctcagaggat gggagctcat 2581 gctggaaaac acagcagccc ctagCCATGG TTTAAACCCG CTGATCAGCC TCGACTGTGC 2641 CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG 2701 GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT TGTCTGAGTA 2761 GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG GATTGGGAAG 2821 ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG GAAAGAACCA 2881 GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAAT AACTTCGTAT 2941 AATGTATGCT ATACGAAGTT ATATCGTATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 3001 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGAATTCCTT GAAATACATC 3061 CACAGGTACA CCTCCAATTG ACTCAAATGA TGTCAATTAG TCTATCAGAA GCTTCTAAAG 3121 CCATGACATC ATTTTCTGGA ATTTTCCAAG CTGTTTAAAG GCACAGTCAA CTTAGTGTAT 3181 GTAAACTTCT GACCCACTGG AATTGTGATA CAGTGAATTA TAAGTGAAAT AATCTGTCTG 3241 TAAACAATTG TTGGAAAAAT GACTTGTGTC ATGCACAAAG TAGATGTCCT AACTGACTTG 3301 CCAAAACTAT TGTTTGTTAA CAAGAAATTT GTGGAGTAGT TGAAAAACGA GTTTTAATGA 3361 CTCCAACTTA AGTGTATGTA AACTTCCGAC TTCAACTGTA GGGTACCGAG CTCGAATTCA 3421 CTGGCCGTCG TTTTACAACG TCGTGACTGG GAAAACCCTG GCGTTACCCA ACTTAATCGC 3481 CTTGCAGCAC ATCCCCCTTT CGCCAGCTGG CGTAATAGCG AAGAGGCCCG CACCGATCGC 3541 CCTTCCCAAC AGTTGCGCAG CCTGAATGGC GAATGGCGCC TGATGCGGTA TTTTCTCCTT 3601 ACGCATCTGT GCGGTATTTC ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT 3661 GCCGCATAGT TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT 3721 TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT 3781 CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGACGAA AGGGCCTCGT GATACGCCTA 3841 TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG 3901 GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG 3961 CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT 4021 ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT 4081 GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG 4141 GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA 4201 CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT 4261 GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG 4321 TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT 4381 GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA 4441 CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT 4501 TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA 4561 GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG 4621 CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC 4681 CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT 4741 ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG 4801 GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG 4861 ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA 4921 CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA 4981 ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA 5041 TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG 5101 CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT 5161 GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC 5221 CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG 5281 GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG 5341 GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA 5401 ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC 5461 GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG 5521 AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC 5581 TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC 5641 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT 5701 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC 5761 GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC 5821 CCAATACGCA AACCGCCTCT CCCCGCGCGT TGGCCGATTC ATTAATGCAG CTGGCACGAC 5881 AGGTTTCCCG ACTGGAAAGC GGGCAGTGAG CGCAACGCAA TTAATGTGAG TTAGCTCACT 5941 CATTAGGCAC CCCAGGCTTT ACACTTTATG CTTCCGGCTC GTATGTTGTG TGGAATTGTG 6001 AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA GCTTGCATGC 6061 CTGCAGGTCG ACTCTAGACC TATACAGTTG AAGTCGGAAG TTTACATACA CTTAAGTTGG 6121 AGTCATTAAA ACTCGTTTTT CAACTACTCC ACAAATTTCT TGTTAACAAA CAATAGTTTT 6181 GGCAAGTCAG TTAGGACATC TACTTTGTGC ATGACACAAG TCATTTTTCC AACAATTGTT 6241 TACAGACAGA TTATTTCACT TATAATTCAC TGTATCACAA TTCCAGTGGG TCAGAAGTTT 6301 ACATACACTA AGTTGACTGT GCCTTTAAAC AGCTTGGAAA ATTCCAGAAA ATGATGTCAT 6361 GGCTTTAGAA GCTTCTGATA GACTAATTGA CATCATTTGA GTCAATTGGA GGTGTACCTG 6421 TGGATGTATT TCAAGGGGAT CCAGACATGA TAAGATACAT TGATGAGTTT GGACAAACCA 6481 CAACTAGAAT GCAGCGGCCG CTAGCTCGAG ATCTTAATTA AACTAGTATC GATATAACTT 6541 CGTATAATGT ATGCTATACG AAGTTATATG TACGGGCCAG ATATACGCGT TGACATTGAT 6601 TATTGACTAG GCTTTTGCAA AAAGCTTTGC AAAGATGGAT AAAGTTTTAA ACAGAGAGGA 6661 ATCTTTGCAG CTAATGGACC TTCTAGGTCT TGAAAGGAGT GGGAATT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.