pCMV-3×HA-Trappc9-Neo plasmid

pCMV-3×HA-Trappc9-Neo plasmid

PVT91653

pCMV-3×HA-Trappc9-Neo plasmid Information

Plasmid name: pCMV-3×HA-Trappc9(mouse)-Neo
Alias: NM_029640.2;1810044A24Rik/2900005P22Rik/4632408O18Rik/Ibp/mKIAA1882/Nibp/TRS130
Gene lenght: 2883bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×HA-Trappc9-Neo plasmid Description

pCMV-3×HA-Trappc9-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×HA-Trappc9-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG 661 AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GGGACCGATC CAGCCTCCGG 721 ACTCTAGAGC TAGCgccacc atgTACCCAT ACGACGTACC TGATTATGCA ACTTATCCTT 781 ATGATGTACC TGATTATGCT AGCTATCCTT ATGATGTCCC AGACTACGCA AAGCTTATGT 841 TGTTGGGGCT GAAAAGACAC TACAAGAAGC GGTGCCAAGG ACGCATGCGG AAACACGTTG 901 GGGACTTGTG CCTCCAGGCT GGGATGCTGC AGGACGCCCT GGTACACTAC CACATGTCCG 961 TGGAGCTCCT GCGCTCAGTG AATGACTTCC TGTGGTTAGG GGCTGCCCTG GAGGGGTTGT 1021 GCTCGGCTTC AGTCATTTAT CATTATCCCG GAGGAACTGG TGGCAAGACT GGGGCTCGGA 1081 GACTCCAAGG CAGCTCACTT CCCTCAGAAG CAGCCAACAG ACACCGGCCA GGGGCGCAAG 1141 AAGTTCTCAT TGACCCAGGT GCTCTCACCA CCAACGGCAT AAATCCTGAC ACCAGCACTG 1201 AGATCGGAAG GGCCAAGAAT TGCCTCAGCC CCGAGGACAT CATTGACAAA TACAAAGAGG 1261 CCATCTCCTA TTACAGCAAG TACAAGAACG CCGGAGTGAT TGAGCTGGAA GCCTGTGTCA 1321 AGGCCGTGCG CGTCCTAGCG ATTCAGAAGC GTGGCATGGA GGCTTCGGAG TTTCTTCAGA 1381 ATGCTGTGTA CATCAATCTC CGGCAGCTTT CGGAAGAAGA GAAAATCCAG CGCTACAGCA 1441 TCCTGTCTGA GCTCTACGAG CTGATTGGCT TCCACCGCAA GTCAGCATTC TTCAAGCGAG 1501 TGGCTGCCAT GCAGTGTGTG GCGCCAAGCA TTGCAGAACC TGGCTGGAGG GCCTGTTACA 1561 AGCTCCTTTT GGAGACGCTG CCTGGCTACA GTCTGTCGCT AGATCCCAAG GACTTCAGCA 1621 AAGGCACACA CCGAGGCTGG GCTGCCGTTC AGATGCGTCT GCTGCATGAG CTGGTCTATG 1681 CCTCCCGCAG GATGGGGAAC CCCGCACTGT CCGTGCGGCA CCTCTCCTTC CTCTTGCAGA 1741 CCATGCTGGA CTTCCTGTCT GACCAGGAAA AGAAAGATGT GACTCAAAGC CTGGAGAACT 1801 ACACTGCCAA GTGTCCCGGG ACGATGGAGC CCATCACCCT CCCAGATGGT CTCACACTGC 1861 CTCCCGTGCC GTTTACCAAG TTGCCCATTG TCAGGTGCGT GAAGCTGCTG AGTCTCCCCA 1921 CCAGCCTGCG ACCCCACAAG GTGAAAAGCT TGCTGGGCCA GAGCATGTCC ACCAAGAGCC 1981 CCTTCATCTA TTCGCCAATC ATCGCACATA ACCGTGGAGA AGAACGCAAC AAAAAAATAG 2041 ATTTCCAGTG GGTTCAAGGA GATGTGTGTG AAGTTCAGCT GATGGTGTAC AACCCAATGC 2101 CGTTTGAGCT CCGCGTGGAA AACATGGGGC TTCTCACCAG CGGAGTGGAG TTTGAGTCTC 2161 TTCCTGCAGC GCTTTCCCTC CCTGCGGAAT CTGGTTTGTA CCCGGTGACG CTGGTCGGAG 2221 TCCCACAGAC TACAGGAATG ATCACTGTGA ATGGTTACCA TACCACAGTC TTTGGGGTCT 2281 TCAGCGACTG CCTTCTGGAC AACCTTCCGG GACTGAAGAC GGGCGGCTCC ACCGTGGAAG 2341 TCATCCCTGC CTTGCCAAGA CTGCAGATCA GCACATCGCT GCCCAGGTCC GCACGCTCAC 2401 TGCAGCCTTC TGCCGGCGAT GAGATCGCCA CCAACGTATC TGTTCAGCTG TACAACGGAG 2461 AAACCCAGCA GCTGGCTGTC ACCTTGGAGA ACATCGGACT GGAGCCTCTG GAGCAGCTGG 2521 AGGTCACCTC AAAGCTTCTC ACCACCAAGG AAAAGTTGTA TGGCGACTTC TTGAGCTGGA 2581 AACTGGAAGA GACGCTGGCC CAGTTTCCCC TGCAGCCCGG GAAGGTGGCT ACTTTCACCA 2641 TCAACATCAA GGCAAAGCTG GATTTCTCTT GCCAGGAGAA TCTTCTCCAG GACCTGAGTG 2701 ATGATGGAAT CAGCGTGAGC GGCTTTCCCC TGTCCAGTCC TTTCCGCCAG GTGGTGCGGC 2761 CCCGAGTGGA GAGCAGACCC ACAAACCCTT CTGAGGGCAG CAAGACGGGG GACCTTGGCC 2821 ACGTCAAGAC TCTGGAAGCT GTCCTGAACT TCAAGTACTC TGGAGGCCCA GGCCACGTGG 2881 AAGGGTATTA CAGGAACCTC TCCCTGGGAC TACACGTGGA AGTCGAGCCT TCTGTGTTTT 2941 TTACCCGAGT CAGCACACTT CCAGCAACCA GCACCCGGCA GTGCCACTTG CTCCTTGATG 3001 TCTTCAACTC CACAGAGCAC GAGCTGACAG TCTGCGCCAG GAACAACTCT GAACTTGTCC 3061 TGCATGCCAG TGAGTGCCAG CGAATGGCTA TTCAAGTGGA CAAGTTCAAC TTTGAAAGTG 3121 TCCCAGAATC CCCCGGAGAG AAGGGACACT TTGCAAATCT GAAGCAGCTG GAAGAAGAGA 3181 GACAGGAGGC ACGAGGCCTG GAGATCAGCA GCAAGCTGGA CATCCGCTGG AGAATTCCTT 3241 CTCTGAAGCG CAGTGGTGAG GCGAGCGTGG AGGGCCTCCT GAACCAGCTC ATCCTGGAAC 3301 ACCTGCAGCT GGCACCTCTG CAGTGGGATG TGCTAGTGGA TGGGCAGCCA TGTGACTGTG 3361 AGGTGGCAGC TGCCTGCCAG GTCGGTGACC CCGTGCGCTT GGAGGTGCGG CTGACTAACA 3421 GGAGTCCCCG AAGTGTGGGG CCCTTTGCCC TCACCGTGGT CCCTTTCCAG GACCACCAGA 3481 ACGGTGTGCA CAACTATGAC CTGCATGATG TCATCTCCTT TGTGGGTTCC AGTACCTTCT 3541 ACCTTGACAC GGTACAACCA TCAGGCCAAT CCACCTGCCT TGGGGCCCTC CTCTTCCTCT 3601 ACACAGGTGA CTTCTTCCTC AACATCCGGT TCCACGAGGA CTGCAAGAGC AAGGAGCTGC 3661 CACCCTCCTG GGTTTGCTTA CCCAGCGTGC ATGTGAGGGC CCTCGGGGCA CAGGCCTGAG 3721 AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGTC TAGAGGGCCC CTTAAGGGTT 3781 CGATCCCTAC CGGTTAGTAA TGAGTTTGAT ATCTCGACAA TCAACCTCTG GATTACAAAA 3841 TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG 3901 CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT 3961 TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG 4021 GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT 4081 GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG 4141 CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG 4201 TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC 4261 TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC 4321 GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC 4381 GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GAAACGGGGG AGGCTAACTG AAACACGGAA 4441 GGAGACAATA CCGGAAGGAA CCCGCGCTAT GACGGCAATA AAAAGACAGA ATAAAACGCA 4501 CGGGTGTTGG GTCGTTTGTT CATAAACGCG GGGTTCGGTC CCAGGGCTGG CACTCTGTCG 4561 ATACCCCACC GAGACCCCAT TGGGGCCAAT ACGCCCGCGT TTCTTCCTTT TCCCCACCCC 4621 ACCCCCCAAG TTCGGGTGAA GGCCCAGGGC TCGCAGCCAA CGTCGGGGCG GCAGGCCCTG 4681 CCATAGCAGA TCTGCGCAGC TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG 4741 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC 4801 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC 4861 GTCAAGCTCT AAATCGGGGC ATCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG 4921 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG 4981 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG 5041 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGGGGATTT 5101 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG 5161 GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA 5221 AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG 5281 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC 5341 GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT 5401 TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG 5461 AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT 5521 TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT 5581 GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA 5641 GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT 5701 TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT 5761 ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC 5821 GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT 5881 TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA 5941 TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG 6001 GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC 6061 AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC 6121 CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT 6181 CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA 6241 TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC 6301 CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG 6361 ACTCTGGGGT TCGCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG 6421 ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT 6481 GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA 6541 TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT 6601 TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT 6661 GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT 6721 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG 6781 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT 6841 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG 6901 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 6961 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 7021 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 7081 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 7141 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 7201 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 7261 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG CTCACGCTGT AGGTATCTCA 7321 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 7381 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 7441 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 7501 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGGACAGTA TTTGGTATCT 7561 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 7621 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 7681 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 7741 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 7801 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 7861 GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA 7921 TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC 7981 CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA 8041 ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC 8101 AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA 8161 ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT 8221 TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG 8281 CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC 8341 TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT 8401 CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT 8461 GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC 8521 TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT 8581 CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA 8641 GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA 8701 CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 8761 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 8821 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCGA CGGATCGGGA GATCTCCCGA 8881 TCCCCTATGG TCGACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATCT 8941 GCTCCCTGCT TGTGTGTTGG AGGTCGCTGA GTAGTGCGCG AGCAAAATTT AAGCTACAAC 9001 AAGGCAAGGC TTGACCGACA ATTGCATGAA GAATCTGCTT AGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.