pCRISPomyces-SaCas9 plasmid

pCRISPomyces-SaCas9 plasmid

PVT95114

pCRISPomyces-SaCas9 plasmid Information

Plasmid name: pCRISPomyces-SaCas9
Alias: Plasmid #129553
Gene lenght: 3158bp
Host: bacteria
Use(s): Gene editing
Fragment type: CRISPR
Species: Staphylococcus aureus
Prokaryotic resistance: Apr
Screening marker:
Fluorescent labeling:
Promoter: rpsL
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: M13R
Copy number: Low
Induction:

pCRISPomyces-SaCas9 plasmid Description

pCRISPomyces-SaCas9 A plasmid suitable for gene editing in bacteria.

pCRISPomyces-SaCas9 plasmid Sequence

1 GCCCTGCAGG CGGAAGTCAG GTAGACACGA CTTCCGCTAG TCCTTGCAAG GTCTGCTGAC 61 GTGAGGCGGG GCGGTCGTTT TTGACCGCCC TGCCTTCGTC ATGTAGGCTC GCTCGCTGTG 121 CCTGGCGTGT CATCAGACGC CCAGGTCCCG GTGCCGTGAG GCCCGGGCCA TCGAGCCGGT 181 GGTACGTGGC TGCGGTCCCC TTGTGAGGGC TGCGCGCCGT GTGCTGTCCG GCGCGCACAG 241 CCTTGAATCC ACCCGCGGGG GCCGGCCGGT CTCCGTGAGC TCGAGTAGAC GACGGAGACG 301 TAATGAAGCG CAACTACATC CTGGGGCTGG ACATCGGCAT CACCTCCGTC GGCTACGGCA 361 TCATCGACTA CGAGACGCGC GACGTCATCG ACGCCGGCGT CCGCCTGTTC AAGGAAGCCA 421 ACGTCGAGAA CAACGAGGGC CGCCGCTCCA AGCGCGGCGC CCGCCGCCTG AAGCGCCGCC 481 GCCGCCACCG CATCCAGCGC GTCAAGAAGC TGCTGTTCGA CTACAACCTG CTGACCGACC 541 ACTCCGAGCT GTCCGGCATC AACCCGTACG AGGCCCGCGT CAAGGGCCTG TCCCAGAAGC 601 TGTCCGAGGA AGAGTTCTCC GCCGCCCTGC TGCACCTGGC CAAGCGCCGC GGCGTCCACA 661 ACGTCAACGA GGTGGAAGAG GACACCGGCA ACGAGCTGTC CACCAAGGAG CAGATCAGCC 721 GCAACTCCAA GGCCCTGGAG GAGAAGTACG TCGCCGAGCT GCAACTGGAG CGGCTGAAGA 781 AGGACGGCGA GGTCCGCGGC TCCATCAACC GCTTCAAGAC CTCCGACTAC GTCAAGGAAG 841 CCAAGCAGCT GCTGAAGGTC CAGAAGGCCT ACCACCAGCT GGACCAGTCC TTCATCGACA 901 CCTACATCGA CCTGCTGGAG ACGCGTCGCA CCTACTACGA GGGTCCGGGC GAGGGCTCCC 961 CGTTCGGCTG GAAGGACATC AAGGAGTGGT ACGAGATGCT GATGGGCCAC TGCACCTACT 1021 TCCCGGAGGA GCTGCGCTCC GTCAAGTACG CCTACAACGC CGACCTGTAC AACGCCCTGA 1081 ACGACCTGAA CAACCTGGTC ATCACCCGCG ACGAGAACGA GAAGCTGGAG TACTACGAGA 1141 AGTTCCAGAT CATCGAGAAC GTGTTCAAGC AGAAGAAGAA GCCGACCCTG AAGCAGATCG 1201 CCAAGGAGAT CCTGGTCAAC GAAGAGGACA TCAAGGGCTA CCGCGTCACC TCCACCGGCA 1261 AGCCGGAGTT CACCAACCTG AAGGTCTACC ACGACATCAA GGACATCACC GCCCGCAAGG 1321 AGATCATCGA GAACGCCGAG CTGCTGGACC AGATCGCCAA GATCCTGACG ATCTACCAGT 1381 CCTCCGAGGA CATCCAGGAA GAGCTGACCA ACCTGAACTC CGAGCTGACC CAGGAAGAGA 1441 TCGAGCAGAT CAGCAACCTG AAGGGCTACA CCGGCACCCA CAACCTGTCC CTGAAGGCCA 1501 TCAACCTGAT CCTGGACGAG CTGTGGCACA CCAACGACAA CCAGATCGCC ATCTTCAACC 1561 GCCTGAAGCT GGTCCCGAAG AAGGTGGACC TGTCCCAGCA GAAGGAGATC CCGACCACCC 1621 TGGTGGACGA CTTCATCCTG TCCCCGGTCG TCAAGCGCTC CTTCATCCAG TCCATCAAGG 1681 TCATCAACGC CATCATCAAG AAGTACGGCC TGCCGAACGA CATCATCATC GAGCTGGCCC 1741 GCGAGAAGAA CTCCAAGGAC GCCCAGAAGA TGATCAACGA GATGCAGAAG CGCAACCGCC 1801 AGACCAACGA GCGCATCGAG GAGATCATCC GCACCACCGG CAAGGAGAAC GCCAAGTACC 1861 TGATCGAGAA GATCAAGCTG CACGACATGC AGGAAGGCAA GTGCCTGTAC TCCCTGGAGG 1921 CCATCCCGCT GGAGGACCTG CTGAACAACC CGTTCAACTA CGAGGTGGAC CACATCATCC 1981 CGCGCTCCGT CTCCTTCGAC AACTCCTTCA ACAACAAGGT CCTGGTCAAG CAGGAAGAGA 2041 ACTCCAAGAA GGGCAACCGC ACCCCGTTCC AGTACCTGTC CTCCTCCGAC TCCAAGATCA 2101 GCTACGAGAC GTTCAAGAAG CACATCCTGA ACCTGGCCAA GGGCAAGGGC CGCATCTCCA 2161 AGACCAAGAA GGAGTACCTG CTGGAGGAGC GCGACATCAA CCGCTTCTCC GTCCAGAAGG 2221 ACTTCATCAA CCGCAACCTG GTGGACACCC GGTACGCCAC CCGCGGCCTG ATGAACCTGC 2281 TGCGCTCCTA CTTCCGCGTC AACAACCTGG ACGTCAAGGT CAAGTCCATC AACGGCGGCT 2341 TCACCTCCTT CCTGCGTCGC AAGTGGAAGT TCAAGAAGGA GCGCAACAAG GGCTACAAGC 2401 ACCACGCCGA GGACGCCCTG ATCATCGCCA ACGCCGACTT CATCTTCAAG GAGTGGAAGA 2461 AGCTGGACAA GGCCAAGAAG GTCATGGAGA ACCAGATGTT CGAGGAGAAG CAGGCCGAGT 2521 CCATGCCGGA GATCGAGACG GAGCAGGAGT ACAAGGAAAT CTTCATCACC CCGCACCAGA 2581 TCAAGCACAT CAAGGACTTC AAGGACTACA AGTACTCCCA CCGCGTGGAC AAGAAGCCGA 2641 ACCGCGAGCT GATCAACGAC ACCCTGTACT CCACCCGCAA GGACGACAAG GGCAACACCC 2701 TGATCGTCAA CAACCTGAAC GGCCTGTACG ACAAGGACAA CGACAAGCTG AAGAAGCTGA 2761 TCAACAAGTC CCCGGAGAAG CTGCTGATGT ACCACCACGA CCCGCAGACC TACCAGAAGC 2821 TGAAGCTGAT CATGGAGCAG TACGGCGACG AGAAGAACCC GCTGTACAAG TACTACGAGG 2881 AGACGGGCAA CTACCTGACG AAGTACTCCA AGAAGGACAA CGGCCCGGTC ATCAAGAAGA 2941 TCAAGTACTA CGGCAACAAG CTGAACGCGC ACCTGGACAT CACCGACGAC TACCCGAACT 3001 CCCGCAACAA GGTCGTCAAG CTGTCCCTGA AGCCGTACCG CTTCGACGTC TACCTGGACA 3061 ACGGCGTCTA CAAGTTCGTC ACCGTCAAGA ACCTGGACGT CATCAAGAAG GAGAACTACT 3121 ACGAGGTCAA CTCCAAGTGC TACGAGGAAG CCAAGAAGCT GAAGAAGATC AGCAACCAGG 3181 CCGAGTTCAT CGCCTCCTTC TACAACAACG ACCTGATCAA GATCAACGGC GAGCTGTACC 3241 GCGTCATCGG CGTCAACAAC GACCTGCTGA ACCGCATCGA GGTCAACATG ATCGACATCA 3301 CCTACCGCGA GTACCTGGAG AACATGAACG ACAAGCGCCC GCCGCGCATC ATCAAGACGA 3361 TCGCCTCCAA GACCCAGTCC ATCAAGAAGT ACTCCACCGA CATCCTGGGC AACCTGTACG 3421 AGGTCAAGTC CAAGAAGCAC CCGCAGATCA TCAAGAAGGG CTGAGAATTC AGATCTACGC 3481 GTTCCCCGCA AAAGCGGCCT TTGACTCCCT GCAAGCCTCA GCGACCGAAT ATATCGGTTA 3541 TGCGTGGGCG ATGGTTGTTG TCATTGTCGG CGCAACTATC GGTATCAAGC TGTTTAAGAA 3601 ATTCACCTCG AAAGCAAGCT GATAAACCGA TACAATTAAA GGCTCCTTTT GGAGCCTTTT 3661 TTTGCTGCTC CTTCGGTCGG ACGTGCGTCT ACGGGCACCT TACCGCAGCC GTCGGCTGTG 3721 CGACACGGAC GGATCGGGCG AACTGGCCGA TGCTGGGAGA AGCGCGCTGC TGTACGGCGC 3781 GCACCGGGTG CGGAGCCCCT CGGCGAGCGG TGTGAAACTT CTGTGAATGG CCTGTTCGGT 3841 TGCTTTTTTT ATACGGCTGC CAGATAAGGC TTGCAGCATC TGGGCGGCTA CCGCTATGAT 3901 CGGGGCGTTC CTGCAATTCT TAGTGCGAGT ATCTGAAAGG GGATACGCAA GTCTTCTCAG 3961 CCGCTACAGG GCGCGTCCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGGGATCG 4021 GTGCGGGCCT CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA 4081 AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGAGCGC 4141 GCGTAATACG ACTCACTATA GGGCGAATTG GGTACCGGGC CCCCCCTCGA GGTCCTCCAG 4201 CTTTTGTTCC CTTTAGTGAG GGTTAATTGC GCGCTTGGCG TAATCATGGT CATAGCTGTT 4261 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA 4321 GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT 4381 GCCCGCTTTC CACCGGTGAA GACATGTTTT AGTACTCTGG AAACAGAATC TACTAAAACA 4441 AGGCAAAATG CCGTGTTTAT CTCGTCAACT TGTTGGCGAG AACTCCATCT GGATTTGTTC 4501 AGAACGCTCG GTTGCCGCCG GGCGTTTTTT ATCTAGAGCT AGCATGCATA TGAAGCTTTG 4561 TTCACATTCG AACGGTCTCT GCTTTGACAA CATGCTGTGC GGTGTTGTAA AGTCGTGGCC 4621 AGGAGAATAC GACAGCGTGC AGGACTGGGG GAGTTATGAC TAGTGTGAAA AGTCAGTATC 4681 CAGTCGTGTA GTTCTTATTA CCTTCTAGAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG 4741 GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG 4801 AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC 4861 GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG 4921 GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT 4981 CGCTCCAAGC TGGGCTGTGT GCACGACCCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC 5041 GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC 5101 ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG 5161 TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA 5221 GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC 5281 GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT 5341 CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT 5401 TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTGGTTCA TGTGCAGCTC 5461 CATCAGCAAA AGGGGATGAT AAGTTTATCA CCACCGACTA TTTGCAACAG TGCCGTTGAT 5521 CGTGCTATGA TCGACTGATG TCATCAGCGG TGGAGTGCAA TGTCGTGCAA TACGAATGGC 5581 GAAAAGCCGA GCTCATCGGT CAGCTTCTCA ACCTTGGGGT TACCCCCGGC GGTGTGCTGC 5641 TGGTCCACAG CTCCTTCCGT AGCGTCCGGC CCCTCGAAGA TGGGCCACTT GGACTGATCG 5701 AGGCCCTGCG TGCTGCGCTG GGTCCGGGAG GGACGCTCGT CATGCCCTCG TGGTCAGGTC 5761 TGGACGACGA GCCGTTCGAT CCTGCCACGT CGCCCGTTAC ACCGGACCTT GGAGTTGTCT 5821 CTGACACATT CTGGCGCCTG CCAAATGTAA AGCGCAGCGC CCATCCATTT GCCTTTGCGG 5881 CAGCGGGGCC ACAGGCAGAG CAGATCATCT CTGATCCATT GCCCCTGCCA CCTCACTCGC 5941 CTGCAAGCCC GGTCGCCCGT GTCCATGAAC TCGATGGGCA GGTACTTCTC CTCGGCGTGG 6001 GACACGATGC CAACACGACG CTGCATCTTG CCGAGTTGAT GGCAAAGGTT CCCTATGGGG 6061 TGCCGAGACA CTGCACCATT CTTCAGGATG GCAAGTTGGT ACGCGTCGAT TATCTCGAGA 6121 ATGACCACTG CTGTGAGCGC TTTGCCTTGG CGGACAGGTG GCTCAAGGAG AAGAGCCTTC 6181 AGAAGGAAGG TCCAGTCGGT CATGCCTTTG CTCGGTTGAT CCGCTCCCGC GACATTGTGG 6241 CGACAGCCCT GGGTCAACTG GGCCGAGATC CGTTGATCTT CCTGCATCCG CCAGAGGCGG 6301 GATGCGAAGA ATGCGATGCC GCTCGCCAGT CGATTGGCTG AGCTCATGAG CGGAGAACGA 6361 GATGACGTTG GAGGGGCAAG GTCGCGCTGA TTGCTGGGGC AACACGTGGA GCGGATCGGG 6421 GATTGTCTTT CTTCAGCTCG CTGATGATAT GCTGACGCTC AATGCCGTTT GGCCTCCGAC 6481 TAACGAAAAT CCCGCATTTG GACGGCTGAT CCGATTGGCA CGGCGGACGG CGAATGGCGG 6541 AGCAGACGCT CGTCCGGGGG CAATGAGATA TGAAAAAGCC TGAACTCACC GCGACGTATC 6601 GATGTCGAGG TTCCTCAGGG GAGCCACCCC AGAGAAGCCC TCGGAGCTGA GCGGAGCTAT 6661 TTCCAAAGCC ATGCCAGCTA GAGACAGTGC ACACCGCCAA GCATCTGCAA AACCCTCGCC 6721 TGGAGGGAAA GTGCAATGTA CGTACGCTGG TTTCCCTCCA GAAAGGGATT CTGCGGCTTC 6781 TCAACTTTGG AAGAAGAGGC GGAACGAACT GCTGTTTCAG CCTACTCATG TGAGGAGGCT 6841 GGTCCTTTAC CCTGATGCCC GAGGGCATCA GGGTAAAGGA CCAGCCTCGC CAACTAGGAG 6901 CGCTACCAAG GCGAACACAA CCCAGGACTT GGTAGAACCC TTCGCCGGAG AAGTTCAGAC 6961 TCATCGGCAT GAGCACCCCT CGCCGCGCAC TCGGCACCGG TCCGTCGACC ACCACCAGGA 7021 CGTCGTTGTC GACGTCGGCC CCGCGGCTCC TGCCCGCCGA ACGCGTCGTC GTCGACGGCC 7081 TGGTGCTCAT CGACGAGCAC CCGGAGCCAG GTGAAAAGCG CCGGCGGACG CTCGGACTGG 7141 GCGCGGGATT CCAGCAGTAA CCCAGGTCCG CCGGCCACCT CACGGCAGGC AGACCCTCGG 7201 CTTTCGCGCC GGGACCGCCA TGAGACCGCC ACCCGGATGT CCGGGGTGGC GGTCTCATGG 7261 CGGTCCCTCA GCGGCCCTAC GCGACCGCTG TGTCGACGCG GAGGCAGTCT CCGGGGTGCT 7321 GGCCCCAGGG CTGGAGTACC GGGGCGAGCT TGCCCTTGCA GCGGCGGCAC ACGGGCGACG 7381 CGGCGCCGGG CGGGGGAGTC TTGACGTCGA CGGTGACCGG CTTCGGCTTC AGGCGCTTCC 7441 GAAGGCTGAT CGTCGGGCGG ATCGCCTCCT TCTTCGGCTG ACGCACTCGG TCGAGCGAAG 7501 CGGCCAGCTC CTCCGTGCGG GCCTCGTTGG CCTTCCGGCG CGCCTCGCGG AAAGCAGCTT 7561 CCTCCTCGGA CATGACCTCG AACCTCATCT GGTCAGCGTC AAGGTCGCCC GGCGCGGCCG 7621 GCGCTTCCGG GGCGGGCGGG TCCAGGACGT CCTTGCCCCA CACCAGGCCC CAGGACTCGA 7681 CGAGCCGCCG GACGCCCGGT AGGCCGTACG TCTCGGCGAC CTTGATGAGG TCGAGGCGAC 7741 GTCCGGCGAC GCGGGCGATG TATCGGTACC AGATGTAGGC CGGGATGACC GCGATGGCGA 7801 CCAGGCCCTC GGTGTCGTCG GTGATCTCCT CCTCGGTGCG GACGTCCTGC TGGATGCCGA 7861 GTTCCTTGAT CAGCCGGTTC AGGTTCTGCG ACCGGTAGTG CTTGCGGACC TGGAACACGC 7921 CGAACTCGCG CTCGCGGTAC TTCTCGACGA ACGGGCCGGG CCGACGAAGC CGCTGCAGCT 7981 CGGCGGCCGC CGCGTCGCCC AGGTCGAGCG GTCCCATGCG GTCGTCGCCG CGACCGGCCT 8041 TGAAGTTCTG TCCGGCCAGC TCCAGGCCGA TCTTGGCGAC GCCGCCCTTG GTCTTGTCGC 8101 CGTCCTTGTA GAGGTAGCGG GCCTGCTTGC CCGCATCGCC GTCAGCGGCG TCCGCGCCGT 8161 TGAGTGGGCG CACGTCGGTG CCGTGGCCCT TGCCCTCACA GGAGCAACCG GGCCGGTCGC 8221 ACGTCTCGCT GACGGTGTAG CCGCCCGCGG ATTCGACCCC GGCGGCCCAG GCTCCGGCGA 8281 GTGCGTCGCG GAACGCGGCC TGGGCGTCCG GGCCGAGCAC CTCGCGGGTG ACCCAGAGCG 8341 TGTGCCAGTG CAGGTGCCAG CCGGAGCCCC AGCCGAAGGT GTCCTCGAAG GCCCGCTCGT 8401 AGCCGATGAT CCCGAAGTCG TCGCGCATCG TGCGCCAGCG GCGGCCGGAC GAGCCGTACG 8461 CGCCCTTCCA GCCGTCGTGC AAGACCGCGA CCAGGCCGTG CCGCATTCCC TTGCGGACGG 8521 TGCCGAACGC CATGCGCTCG AAGTGGCGCA ACGTGTTCGT GCCAAGGTGC AGCCCGTACC 8581 CGGCGTCCGC GAGACCGTCG GCGGCGAGCT GCACGTTCGA GCCCCGTACG GCCAGGATGC 8641 GGCTCATGCA CCACGGGCAG GTGTGGACGT TGTTGCAGCG GCACGTGTTG CCCCACGTCG 8701 CCTCGCCCGG CTTCCACATC AGCTCGGCCG TCCCGGCAGT GAGCCGGGTC CCGCAGCCCT 8761 TGAACGCCTC GTTCAGCGAC ACCGTCTGGT GCCGGTCCCG CCGGGCGAAC CGCTCGTCGC 8821 GCGGGTCCTC CCGCCCGGCT GTCGCGGCAC CCTCGTTTGG GGTAGAACCC GTTCCAGTTA 8881 CAGCGCTCTG ACCTGCAGTG GACGGAGATT TTCCCTTACT ACTAAAGCCC GCGTCCGGAT 8941 TACCCGCTGT AGTCGTGCTT GCTACGCTGC GTGACTGGTC CGCAATGAGA CGCTTTGCGC 9001 GCTTTCGGCA GGCGTCCGAG CAGTAGATTT TGGGGCGCTT CCCGGGGATG TGGACGATCG 9061 GGGTGCCGCA GTGGCACTTC GGTCCGGCGG GGCGCGGTGG TGTCGACGCG CTGTTCTCTC 9121 GTACGCTCGT CACAGAGCAA ACGTCCTCAC TCGGCATGCT GCGCCGGTTC GGGGGCGGCG 9181 AGCCCGGGAG GCCAATCCCG GGCTCGTGCC ATTTCTGGGT CCTGTTGATC CTGGCATTGG 9241 TGTGGCCGTT CATTGCCCCT GCTCGCTCCT GACGCGCCGA TAGACGTCCG ATACGCCCGG 9301 TGCTGGTGGG ATTTGATAGG TCGGAAGAAG CCCCGCCCGG GCCTGGGCGG GGCTTCCTGT 9361 GCGTCAGGAC CTCCTCGTCG TGAGCCTCTT CGGCCTATGG ACGGAGTGAC CTCGTGATCC 9421 GTTACAGCCG CGCGCGCTCG CGTAGAGCGG TCTCATCAGT TCCACGAACG GTCCTCTTCG 9481 CAGATCAGGG CGTTGGGGCG GAGTCTCACC AAGGACTACG TCTGCTGGCG ATTTCCGTTA 9541 CACCCCGGGC GGTGGCCGGC GCACACGCGC GCCCGCGTTG GGCAGTGCAG AAAGTGCAGA 9601 AACCTAGGCG CTGATGGTCC AGGTCCACGG TTCGTCGTCG GCGGCGGCGC GGGCGGCGGC 9661 GTCGGCCAGG GCGCGGGCGA GACCGGCTAC GGCGGGCTTG ATGCGCCGGT TGCGGGCGAC 9721 CTTGAGCAGC TAGTATGCAG GTCGACGGAT CTTTTCCGCT GCATAACCCT GCTTCGGGGT 9781 CATTATAGCG ATTTTTTCGG TATATCCATC CTTTTTCGCA CGATATACAG GATTTTGCCA 9841 AAGGGTTCGT GTAGACTTTC CTTGGTGTAT CCAACGGCGT CAGCCGGGCA GGATAGGTGA 9901 AGTAGGCCCA CCCGCGAGCG GGTGTTCCTT CTTCACTGTC CCTTATTCGC ACCTGGCGGT 9961 GCTCAACGGG AATCCTGCTC TGCGAGGCTG GCCGGCTACC GCCGGCGTAA CAGATGAGGG 10021 CAAGCGGATG GCTGATGAAA CCAAGCCAAC CAGGAAGGGC AGCCCACCTA TCAAGGTGTA 10081 CTGCCTTCCA GACGAACGAA GAGCGATTGA GGAAAAGGCG GCGGCGGCCG GCATGAGCCT 10141 GTCGGCCTAC CTGCTGGCCG TCGGCCAGGG CTACAAAATC ACGGGCGTCG TGGACTATGA 10201 GCACGTCCGC GAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.