pCRISPomyces-2-FM21-sgRNA plas

pCRISPomyces-2-FM21-sgRNA plasmid

PVT95067

pCRISPomyces-2-FM21-sgRNA plasmid Information

Plasmid name: pCRISPomyces-2-FM21-sgRNA
Alias:
Gene lenght:
Host: E.coli
Use(s): Gene editing
Fragment type: CRISPR
Species: Streptococcus
Prokaryotic resistance: Apr
Screening marker:
Fluorescent labeling:
Promoter: gapdh
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: Forward left ITR
Reverse primer:
Copy number: High
Induction:

pCRISPomyces-2-FM21-sgRNA plasmid Description

pCRISPomyces-2-FM21-sgRNA is a plasmid suitable for gene editing in Escherichia coli.

pCRISPomyces-2-FM21-sgRNA plasmid Sequence

1 gccctgcagg cggaagtcag gtagacacga cttccgctag tccttgcaag gtctgctgac 61 gtgaggcggg gcggtcgttt ttgaccgccc tgccttcgtc atgtaggctc gctcgctgtg 121 cctggcgtgt catcagacgc ccaggtcccg gtgccgtgag gcccgggcca tcgagccggt 181 ggtacgtggc tgcggtcccc ttgtgagggc tgcgcgccgt gtgctgtccg gcgcgcacag 241 ccttgaatcc acccgcgggg gccggccggt ctccgtgagc tcgagtagac gacggagacg 301 taatggacaa gaagtacagc atcggcctgg acatcggcac caacagcgtg ggctgggcgg 361 tcatcaccga cgagtacaag gtcccctcca agaagttcaa ggtcctgggc aacaccgacc 421 ggcactcgat caagaagaac ctgatcggcg ccctgctctt cgacagcggc gaaaccgccg 481 aggcgacccg cctgaagcgg accgcccgtc gccgctacac ccggcgcaag aaccgcatct 541 gctacctgca ggagatcttc tccaacgaga tggccaaggt cgacgactcg ttcttccacc 601 ggctcgagga gagcttcctg gtggaggagg acaagaagca cgagcgccac ccgatcttcg 661 gcaacatcgt cgacgaggtg gcctaccacg agaagtaccc caccatctac cacctccgca 721 agaagctggt cgactcgacc gacaaggcgg acctgcggct catctacctg gccctcgcgc 781 acatgatcaa gttccgcggc cacttcctca tcgagggcga cctgaacccg gacaactccg 841 acgtcgacaa gctcttcatc cagctggtgc agacctacaa ccagctgttc gaggagaacc 901 ccatcaacgc cagcggcgtc gacgccaagg cgatcctctc cgcgcgcctg agcaagtccc 961 ggcgcctgga gaacctcatc gcccagctgc cgggcgagaa gaagaacggc ctcttcggca 1021 acctgatcgc gctgtcgctc ggcctgaccc ccaacttcaa gagcaacttc gacctggccg 1081 aggacgcgaa gctccagctg tccaaggaca cctacgacga cgacctggac aacctgctcg 1141 cccagatcgg cgaccagtac gcggacctct tcctggccgc gaagaacctc tcggacgcca 1201 tcctgctcag cgacatcctg cgggtcaaca ccgagatcac caaggccccg ctgtcggcga 1261 gcatgatcaa gcggtacgac gagcaccacc aggacctgac cctgctcaag gccctcgtgc 1321 gccagcagct gcccgagaag tacaaggaga tcttcttcga ccagtccaag aacggctacg 1381 ccggctacat cgacggcggc gcgtcgcagg aggagttcta caagttcatc aagccgatcc 1441 tggagaagat ggacggcacc gaggagctgc tcgtcaagct gaaccgcgag gacctgctcc 1501 gcaagcagcg gaccttcgac aacggctcca tcccgcacca gatccacctg ggcgagctcc 1561 acgccatcct ccggcgccag gaggacttct accccttcct gaaggacaac cgcgagaaga 1621 tcgagaagat cctgaccttc cggatcccgt actacgtcgg ccccctggcc cgcggcaact 1681 cccggttcgc gtggatgacc cggaagtcgg aggaaaccat caccccgtgg aacttcgagg 1741 aggtcgtgga caagggcgcc tccgcgcagt cgttcatcga gcgcatgacc aacttcgaca 1801 agaacctccc gaacgagaag gtcctgccca agcacagcct gctctacgag tacttcaccg 1861 tgtacaacga gctgaccaag gtcaagtacg tgaccgaggg catgcggaag ccggccttcc 1921 tgtccggcga gcagaagaag gcgatcgtcg acctgctctt caagaccaac cgcaaggtca 1981 ccgtgaagca gctgaaggag gactacttca agaagatcga gtgcttcgac tccgtcgaga 2041 tctcgggcgt ggaggaccgc ttcaacgcct ccctgggcac ctaccacgac ctgctcaaga 2101 tcatcaagga caaggacttc ctcgacaacg aggagaacga ggacatcctg gaggacatcg 2161 tcctcaccct gaccctcttc gaggaccgcg agatgatcga ggagcggctc aagacctacg 2221 cccacctgtt cgacgacaag gtgatgaagc agctgaagcg gcgccggtac accggctggg 2281 gccgcctctc ccggaagctg atcaacggca tccgggacaa gcagagcggc aagaccatcc 2341 tggacttcct caagtccgac ggcttcgcca accgcaactt catgcagctc atccacgacg 2401 actcgctgac cttcaaggag gacatccaga aggcccaggt gtccggccag ggcgacagcc 2461 tccacgagca catcgccaac ctggcgggct ccccggcgat caagaagggc atcctccaga 2521 ccgtcaaggt cgtggacgag ctggtcaagg tgatgggccg ccacaagccc gagaacatcg 2581 tgatcgagat ggcccgggag aaccagacca cccagaaggg ccagaagaac tcccgcgagc 2641 ggatgaagcg catcgaggag ggcatcaagg agctcggctc gcagatcctg aaggagcacc 2701 cggtcgagaa cacccagctc cagaacgaga agctgtacct ctactacctg cagaacggcc 2761 gcgacatgta cgtggaccag gagctcgaca tcaaccggct gagcgactac gacgtcgacc 2821 acatcgtgcc gcagtccttc ctgaaggacg actcgatcga caacaaggtc ctgacccgct 2881 ccgacaagaa ccggggcaag tccgacaacg tgccctcgga ggaggtcgtg aagaagatga 2941 agaactactg gcgccagctg ctcaacgcca agctcatcac ccagcgcaag ttcgacaacc 3001 tgaccaaggc cgagcggggc ggcctgtcgg agctcgacaa ggcgggcttc atcaagcgcc 3061 agctcgtcga aacccggcag atcaccaagc acgtggccca gatcctggac agccggatga 3121 acaccaagta cgacgagaac gacaagctga tccgcgaggt caaggtgatc accctcaaga 3181 gcaagctggt gtccgacttc cgcaaggact tccagttcta caaggtccgg gagatcaaca 3241 actaccacca cgcccacgac gcgtacctga acgccgtcgt gggcaccgcg ctgatcaaga 3301 agtacccgaa gctggagtcc gagttcgtct acggcgacta caaggtctac gacgtgcgca 3361 agatgatcgc caagtcggag caggagatcg gcaaggccac cgcgaagtac ttcttctaca 3421 gcaacatcat gaacttcttc aagaccgaga tcaccctggc caacggcgag atccgcaagc 3481 ggcccctgat cgaaaccaac ggcgaaaccg gcgagatcgt ctgggacaag ggccgcgact 3541 tcgccaccgt ccggaaggtg ctgtccatgc cgcaggtcaa catcgtcaag aaaaccgagg 3601 tgcagaccgg cggcttcagc aaggagtcca tcctccccaa gcgcaactcg gacaagctga 3661 tcgcccggaa gaaggactgg gacccgaaga agtacggcgg cttcgacagc cccaccgtcg 3721 cctactccgt gctggtcgtg gcgaaggtcg agaagggcaa gagcaagaag ctgaagtccg 3781 tgaaggagct gctcggcatc accatcatgg agcgctcctc gttcgagaag aacccgatcg 3841 acttcctgga ggccaagggc tacaaggagg tcaagaagga cctcatcatc aagctgccca 3901 agtactcgct gttcgagctc gagaacggcc gcaagcggat gctcgccagc gcgggcgagc 3961 tgcagaaggg caacgagctg gccctcccgt ccaagtacgt caacttcctg tacctcgcgt 4021 cccactacga gaagctgaag ggctcgcccg aggacaacga gcagaagcag ctcttcgtgg 4081 agcagcacaa gcactacctg gacgagatca tcgagcagat ctcggagttc agcaagcggg 4141 tcatcctggc cgacgcgaac ctcgacaagg tgctgtccgc ctacaacaag caccgcgaca 4201 agccgatccg ggagcaggcg gagaacatca tccacctgtt caccctcacc aacctgggcg 4261 cccccgccgc gttcaagtac ttcgacacca ccatcgaccg caagcggtac accagcacca 4321 aggaggtcct cgacgcgacc ctgatccacc agtccatcac cggcctgtac gaaacccgca 4381 tcgacctctc ccagctcggc ggcgactgag aattcagatc tacgcgttcc ccgcaaaagc 4441 ggcctttgac tccctgcaag cctcagcgac cgaatatatc ggttatgcgt gggcgatggt 4501 tgttgtcatt gtcggcgcaa ctatcggtat caagctgttt aagaaattca cctcgaaagc 4561 aagctgataa accgatacaa ttaaaggctc cttttggagc ctttttttgc tgctccttcg 4621 gtcggacgtg cgtctacggg caccttaccg cagccgtcgg ctgtgcgaca cggacggatc 4681 gggcgaactg gccgatgctg ggagaagcgc gctgctgtac ggcgcgcacc gggtgcggag 4741 cccctcggcg agcggtgtga aacttctgtg aatggcctgt tcggttgctt tttttatacg 4801 gctgccagat aaggcttgca gcatctgggc ggctaccgct atgatcgggg cgttcctgca 4861 attcttagtg cgagtatctg aaaggggata cgcaaGAGAC ATCTTTGAAG ACAAacgcAC 4921 AGCTCGACGG CGATCACGgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg 4981 ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt agcataaccc cttggggcct 5041 ctaaacgggt cttgaggggt tttttggctg ctccttcggt cggacgtgcg tctacgggca 5101 ccttaccgca gccgtcggct gtgcgacacg gacggatcgg gcgaactggc cgatgctggg 5161 agaagcgcgc tgctgtacgg cgcgcaccgg gtgcggagcc cctcggcgag cggtgtgaaa 5221 cttctgtgaa tggcctgttc ggttgctttt tttatacggc tgccagataa ggcttgcagc 5281 atctgggcgg ctaccgctat gatcggggcg ttcctgcaat tcttagtgcg agtatctgaa 5341 aggggatacg cCGCCGCCGA CCAGCCCATG GgtttAAGTC TTCTTTCACG TGGCatgttt 5401 tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca 5461 ccgagtcggt gctttttact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg 5521 ttttttatct agaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 5581 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 5641 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 5701 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 5761 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 5821 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 5881 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 5941 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 6001 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 6061 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 6121 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 6181 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 6241 caaaaaggat cttcacctag atccttttgg ttcatgtgca gctccatcag caaaagggga 6301 tgataagttt atcaccaccg actatttgca acagtgccgt tgatcgtgct atgatcgact 6361 gatgtcatca gcggtggagt gcaatgtcgt gcaatacgaa tggcgaaaag ccgagctcat 6421 cggtcagctt ctcaaccttg gggttacccc cggcggtgtg ctgctggtcc acagctcctt 6481 ccgtagcgtc cggcccctcg aagatgggcc acttggactg atcgaggccc tgcgtgctgc 6541 gctgggtccg ggagggacgc tcgtcatgcc ctcgtggtca ggtctggacg acgagccgtt 6601 cgatcctgcc acgtcgcccg ttacaccgga ccttggagtt gtctctgaca cattctggcg 6661 cctgccaaat gtaaagcgca gcgcccatcc atttgccttt gcggcagcgg ggccacaggc 6721 agagcagatc atctctgatc cattgcccct gccacctcac tcgcctgcaa gcccggtcgc 6781 ccgtgtccat gaactcgatg ggcaggtact tctcctcggc gtgggacacg atgccaacac 6841 gacgctgcat cttgccgagt tgatggcaaa ggttccctat ggggtgccga gacactgcac 6901 cattcttcag gatggcaagt tggtacgcgt cgattatctc gagaatgacc actgctgtga 6961 gcgctttgcc ttggcggaca ggtggctcaa ggagaagagc cttcagaagg aaggtccagt 7021 cggtcatgcc tttgctcggt tgatccgctc ccgcgacatt gtggcgacag ccctgggtca 7081 actgggccga gatccgttga tcttcctgca tccgccagag gcgggatgcg aagaatgcga 7141 tgccgctcgc cagtcgattg gctgagctca tgagcggaga acgagatgac gttggagggg 7201 caaggtcgcg ctgattgctg gggcaacacg tggagcggat cggggattgt ctttcttcag 7261 ctcgctgatg atatgctgac gctcaatgcc gtttggcctc cgactaacga aaatcccgca 7321 tttggacggc tgatccgatt ggcacggcgg acggcgaatg gcggagcaga cgctcgtccg 7381 ggggcaatga gatatgaaaa agcctgaact caccgcgacg tatcgatgtc gaggttcctc 7441 aggggagcca ccccagagaa gccctcggag ctgagcggag ctatttccaa agccatgcca 7501 gctagagaca gtgcacaccg ccaagcatct gcaaaaccct cgcctggagg gaaagtgcaa 7561 tgtacgtacg ctggtttccc tccagaaagg gattctgcgg cttctcaact ttggaagaag 7621 aggcggaacg aactgctgtt tcagcctact catgtgagga ggctggtcct ttaccctgat 7681 gcccgagggc atcagggtaa aggaccagcc tcgccaacta ggagcgctac caaggcgaac 7741 acaacccagg acttggtaga acccttcgcc ggagaagttc agactcatcg gcatgagcac 7801 ccctcgccgc gcactcggca ccggtccgtc gaccaccacc aggacgtcgt tgtcgacgtc 7861 ggccccgcgg ctcctgcccg ccgaacgcgt cgtcgtcgac ggcctggtgc tcatcgacga 7921 gcacccggag ccaggtgaaa agcgccggcg gacgctcgga ctgggcgcgg gattccagca 7981 gtaacccagg tccgccggcc acctcacggc aggcagaccc tcggctttcg cgccgggacc 8041 gccatgagac cgccacccgg atgtccgggg tggcggtctc atggcggtcc ctcagcggcc 8101 ctacgcgacc gctgtgtcga cgcggaggca gtctccgggg agccggcccc agggctggag 8161 taccggggcg agcttgccct tgcagcggcg gcacacgggc gacgcggcgc cgggcggggg 8221 agtcttgacg tcgacggtga ccggcttcgg cttcaggcgc ttccgaaggc tgatcgtcgg 8281 gcggatcgcc tccttcttcg gctgacgcac tcggtcgagc gaagcggcca gctcctccgt 8341 gcgggcctcg ttggccttcc ggcgcgcctc gcggaaagca gcttcctcct cggacatgac 8401 ctcgaacctc atctggtcag cgtcaaggtc gcccggcgcg gccggcgctt ccggggcggg 8461 cgggtccagg acgtccttgc cacacaccag gccccaggac tcgacgagcc gccggacgcc 8521 cggtaggccg tacgtctcgg cgaccttgat gaggtcgagg cgacgtccgg cgacgcgggc 8581 gatgtatcgg taccagatgt aggccgggat gaccgcgatg gcgaccaggc cctcggtgtc 8641 gtcggtgatc tcctcctcgg tgcggacgtc ctgctggatg ccgagttcct tgatcagccg 8701 gttcaggttc tgcgaccggt agtgcttgcg gacctggaac acgccgaact cgcgctcgcg 8761 gtacttctcg acgaacgggc cgggccgacg aagccgctgc agctcggcgg ccgccgcgtc 8821 gcccaggtcg agcggtccca tgcggtcgtc gccgcgaccg gccttgaagt tctgtccggc 8881 cagctccagg ccgatcttgg agacgccgcc cttggtcttg tcgccgtcct tgtagaggta 8941 gcgggcctgc ttgcccgcat cgccgtcagc ggcgtccgcg ccgttgagtg ggcgcacgtc 9001 ggtgccgtgg cccttgccct cacaggagca accgggccgg tcgcacgtct tgctgacggt 9061 gtagccgccc gcggattcga ccccggcggc ccaggctccg gcgagtgcgt cgtggaacgc 9121 ggcctgggcg tccgggccga gcacctcgcg ggtgacccag agcgtgtgcc agtgcaggtg 9181 ccagccggag ccccagccga aggtgtcctc gaaggcccgc tcgtagccga tgatcccgaa 9241 gtcgtcgcgc atcgtgcgcc agcggcggcc ggacgagccg tacgcgccct tccagccgtc 9301 gtgcaagacc gcgaccaggc cgtgccgcat tcccttgcgg acggtgccga acgccatgcg 9361 ctcgaagtgg cgcaacgtgt tcgtgccaag gtgcagcccg tacccggcgt ccgcgagacc 9421 gtcggcggcg agctgcacgt tcgagccccg tacggccagg atgcggctca tgcaccacgg 9481 gcaggtgtgg acgttgttgc agcggcacgt gttgccccac gtcgcctcgc ccggcttcca 9541 catcagctcg gccgtcccgg cagtgagccg ggtcccgcag cccttgaacg cctcgttcag 9601 cgacaccgtc tggtgccggt cccgccgggc gaaccgctcg tcgcgcgggt cctcccgccc 9661 ggctgtcgcg gcaccctcgt ttggggtaga acccgttcca gttacagcgc tctgacctgc 9721 agtggacgga gattttccct tactactaaa gcccgcgtcc ggattacccg ctgtagtcgt 9781 gcttgctacg ctgcgtgact ggtccgcaat gagacgcttt gcgcgctttc ggcaggcgtc 9841 cgagcagtag attttggggc gcttcccggg gatgtggacg atcggggtgc cgcagtggca 9901 cttcggtccg gcggggcgcg gtggtgtcga cgcgctgttc tctcgtacgc tcgtcacaga 9961 gcaaacgtcc tcactcggca tgctgcgcca gttgcggggg cggcgagccc gggaggccaa 10021 tcccgggctc gtgccatttc tgggtcctgt tgatcctggc attggtgtgg ccgttcattg 10081 cccctgctcg ctcctgacgc gccgatagac gtccgatacg cccggtgctg gtgggatttg 10141 ataggtcgga agaagccccg cccgggcctg ggcggggctt cctgtgcgtc aggacctcct 10201 cgtcgtgagc ctcttcggcc tatggacgga gtgacctcgt gatccgttac agccgcgcgc 10261 gctcgcgtag agcggtctca tcagttccac gaacggtcct cttcgcagat cagggcgttg 10321 gggcggagtc tcaccaagga ctacgtctgc tggcgatttc cgttacaccc cgggcggtgg 10381 ccggcgcaca cgcgcgcccg cgttgggcag tgcagaaagt gcagaaacct aggcgctgat 10441 ggtccaggtc cacggttcgt cgtcggcggc ggcgcgggcg gcggcgtcgg ccagggcgcg 10501 ggcgagaccg gctacggcgg gcttgatgcg ccggttgcgg gcgaccttga gcagctagta 10561 tgcaggtcga cggatctttt ccgctgcata accctgcttc ggggtcatta tagcgatttt 10621 ttcggtatat ccatcctttt tcgcacgata tacaggattt tgccaaaggg ttcgtgtaga 10681 ctttccttgg tgtatccaac ggcgtcagcc gggcaggata ggtgaagtag gcccacccgc 10741 gagcgggtgt tccttcttca ctgtccctta ttcgcacctg gcggtgctca acgggaatcc 10801 tgctctgcga ggctggccgg ctaccgccgg cgtaacagat gagggcaagc ggatggctga 10861 tgaaaccaag ccaaccagga agggcagccc acctatcaag gtgtactgcc ttccagacga 10921 acgaagagcg attgaggaaa aggcggcggc ggccggcatg agcctgtcgg cctacctgct 10981 ggccgtcggc cagggctaca aaatcacggg cgtcgtggac tatgagcacg tccgcgagct 11041 g

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.