pLV3-CMV-TRAM2-3×FLAG-CopGFP-P

pLV3-CMV-TRAM2-3×FLAG-CopGFP-Puro plasmid

PVT95099

pLV3-CMV-TRAM2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-TRAM2(human)-3×FLAG-CopGFP-Puro
Alias: NM_012288.4
Gene lenght: 1110bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TRAM2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-TRAM2-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-TRAM2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg ctttccgcag gaggacgaaa 2341 agttacccgc tcttcagcca ggagttcgtc atccacaacc atgcggacat cggcttctgc 2401 ctggtgctct gcgtcctcat cgggcttatg ttcgaggtca cagccaagac tgcctttcta 2461 tttattttac ctcagtataa cattagcgtg cctacagcag acagtgagac cgtgcactac 2521 cactatggcc ctaaggacct ggtcacaatc ttgttctaca tcttcatcac catcatcttg 2581 catgctgtgg ttcaggagta cattttagat aaaatcagca aacggcttca tctctccaaa 2641 gtcaaacaca gcaagttcaa tgaatctgga cagctggtcg tctttcattt cacctcggtg 2701 atttggtgct tctacgtggt ggtgacggaa ggatacttaa caaacccaag aagcctctgg 2761 gaagactacc cgcatgtgca cctccccttc caggtgaagt ttttctacct atgccagctg 2821 gcctactggc tgcacgcact tcctgagcta tacttccaga aggtacggaa ggaggaaatt 2881 ccccgccagc tccagtatat ttgcctgtac ctggtgcata tagctggagc atacctctta 2941 aacctgagcc gcctgggcct gatcttgctg ctgctgcagt actcaactga gttcctcttc 3001 cacacggcta gactcttcta ctttgcagat gaaaacaacg agaaactgtt cagtgcctgg 3061 gctgctgttt ttggggttac ccgcctcttc atcctcaccc ttgccgtgct ggccattggc 3121 tttggactgg ctcgcatgga aaaccaggca tttgatcccg agaaagggaa cttcaacact 3181 ttgttttgca ggctctgcgt gctgctgctg gtgtgtgccg cccaggcctg gctcatgtgg 3241 cgcttcatcc actcccagct gcggcactgg cgggaatact ggaatgagca gagtgcaaag 3301 cggagagtcc cagccacacc cagactacca gccaggctca tcaagaggga atctggttac 3361 catgaaaatg gagtggtgaa ggcagagaac ggaacctccc cacggactaa gaaactcaag 3421 tctcccggat ccGACTACAA AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC 3481 AAGGATGACG ATGACAAGta gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag 3541 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga 3601 acgggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc 3661 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt 3721 ctttttcgca acgggtttgc cgccagaaca cagctgaagc ttcgaggggc tcgcatctct 3781 ccttcacgcg cccgccgccc tacctgaggc cgccatccac gccggttgag tcgcgttctg 3841 ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg tctaggtaag tttaaagctc 3901 aggtcgagac cgggcctttg tccggcgctc ccttggagcc tacctagact cagccggctc 3961 tccacgcttt gcctgaccct gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg 4021 cgccgttaca gatccaagct gtgaccggcg cctacgctag acgccaccat ggagagcgac 4081 gagagcggcc tgcccgccat ggagatcgag tgccgcatca ccggcaccct gaacggcgtg 4141 gagttcgagc tggtgggcgg cggagagggc acccccaagc agggccgcat gaccaacaag 4201 atgaagagca ccaaaggcgc cctgaccttc agcccctacc tgctgagcca cgtgatgggc 4261 tacggcttct accacttcgg cacctacccc agcggctacg agaacccctt cctgcacgcc 4321 atcaacaacg gcggctacac caacacccgc atcgagaagt acgaggacgg cggcgtgctg 4381 cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg 4441 ggcaccggct tccccgagga cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc 4501 accgtggagc acctgcaccc catgggcgat aacgtgctgg tgggcagctt cgcccgcacc 4561 ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg acagccacat gcacttcaag 4621 agcgccatcc accccagcat cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg 4681 gaggagctgc acagcaacac cgagctgggc atcgtggagt accagcacgc cttcaagacc 4741 cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc 4801 ggacccggct ccaccggatc tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg 4861 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac 4921 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc 4981 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc 5041 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg 5101 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc 5161 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg 5221 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac 5281 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc 5341 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc 5401 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc 5461 cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa tttgtgaaag attgactggt 5521 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat 5581 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg 5641 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt 5701 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact 5761 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc 5821 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaATCATCG 5881 TCCTTTCCTt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc 5941 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg 6001 cggcctcttc cgcgtctTcg ccttcgccct cagacgagtc ggatctccct ttggGccgcc 6061 tccccgcctg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt 6121 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaaGat aagatctgct 6181 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 6241 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 6301 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 6361 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 6421 gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata 6481 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 6541 tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctaTCCC GCCCCTAACT 6601 CCGCCCAtcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta 6661 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag 6721 tgaggaggct tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata 6781 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 6841 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 6901 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 6961 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 7021 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 7081 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 7141 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 7201 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 7261 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 7321 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 7381 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 7441 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7501 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 7561 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 7621 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 7681 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 7741 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 7801 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 7861 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 7921 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 7981 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 8041 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 8101 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 8161 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 8221 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 8281 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 8341 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 8401 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 8461 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 8521 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 8581 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 8641 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 8701 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 8761 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 8821 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 8881 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 8941 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 9001 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 9061 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 9121 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 9181 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 9241 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 9301 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 9361 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.