pAAV-TBG-Serpina1e-P2A-GFP pla

pAAV-TBG-Serpina1e-P2A-GFP plasmid

PVT97012

pAAV-TBG-Serpina1e-P2A-GFP plasmid Information

Plasmid name: pAAV-TBG-Serpina1e(mouse)-P2A-GFP
Alias: NM_009247.2;Dom5; PI5; Spi1-5
Gene lenght: 1242bp
Host: Mammalian cells,Adeno associated virus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: TBG
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: TBG-F1
Reverse primer:
Copy number: High
Induction:

pAAV-TBG-Serpina1e-P2A-GFP plasmid Description

pAAV-TBG-Serpina1e-P2A-GFP This is a plasmid suitable for protein expression in mammalian cells and can also be used for adeno-associated virus packaging.

pAAV-TBG-Serpina1e-P2A-GFP plasmid Sequence

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt 61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact 121 aggggttcct GCGGCCGGTC GCGTCTAGTA CTAGTgggct ggaagctacc tttgacatca 181 tttcctctgc gaatgcatgt ataatttcta cagaacctat tagaaaggat cacccagcct 241 ctgcttttgt acaactttcc cttaaaaaac tgccaattcc actgctgttt ggcccaatag 301 tgagaacttt ttcctgctgc ctcttggtgc ttttgcctat ggcccctatt ctgcctgctg 361 aagacactct tgccagcatg gacttaaacc cctccagctc tgacaatcct ctttctcttt 421 tgttttacat gaagggtctg gcagccaaag caatcactca aagttcaaac cttatcattt 481 tttgctttgt tcctcttggc cttggttttg tacatcagct ttgaaaatac catcccaggg 541 ttaatgctgg ggttaattta taactaagag tgctctagtt ttgcaataca ggacatgcta 601 taaaaatgga aagatGGTAC CGAGGAGATC TGCCGCCGCG ATCGCCGGCG GATCCGCATT 661 GCGGCCGCAG ACATATGTCT CACGCTTAAC TAGCTAGCGC CACCatgact ccctccatct 721 catggtgtct actgcttctg gcaggcctgt gttgcctggt ccccagcttt ctggctgagg 781 atgttcagga gacagacacc tcccagaagg atcagtcccc agcctcccat gagattgcta 841 caaacctggg agactttgcc atcagtctat accgggagct ggtccatcag tccaacactt 901 ccaacatctt cttctcccca gtgagcattg ccacagcctt tgctatgctc tccctaggga 961 gcaagggtga cactcacacg cagatcctag agggcctgca gttcaacctc acacaaacat 1021 cggaggctga catccacaat tccttccaac acctcctcca aaccctcaac agaccagaca 1081 gtgagctgca gctgagcaca ggcaatggcc tctttgtcaa caatgacctg aagctggtgg 1141 agaagtttct ggaagaggcc aagaaccatt atcaggcaga agtcttctct gtcaactttg 1201 cagagtcaga ggaggccaag aaagtgatta atgattttgt ggagaaggga acccaaggaa 1261 agatagttga ggctgtgaaa aaactagaac aagacacagt tttcgtcctg gcaaattaca 1321 ttctctttaa aggcaaatgg aagaagccat tcgatcctga gaacactaag caagctgagt 1381 tccacgtgga cgagtccacc acggtgaagg tgcccatgat gaccctctcg ggcatgcttg 1441 acgtgcacca ttgcagcacg ctctccagct gggtgctgct gatggattac gcgggcaacg 1501 ccactgctgt cttcctcctg cccgatgatg ggaagatgca gcatctggag caaactctca 1561 acaaggagct catctctaag ttcctgctaa acaggcgcag aaggttagcc cagatccata 1621 tccccagact gtccatctct ggaaactata acttggagac actcatgagt ccactgggca 1681 tcacccggat cttcaacagt ggggctgacc tctccggaat cacagaggag aatgctcccc 1741 tgaagctcag ccaggctgtg cataaggccg tgctgaccat cgatgagaca ggaacagaag 1801 ctgcagcagc tacagtctta caaggcggtt ttttgtctat gccccctatc ttgcacttca 1861 accgcccttt ccttttcata atatttgaag aacactctca gagccccctc tttgtgggaa 1921 aagtggtaga tcccacacat aaaACGCGTG GAAGCGGAGC TACTAACTTC AGCCTGCTGA 1981 AGCAGGCTGG AGACGTGGAG GAGAACCCTG GACCTGTCGA CATGGTGAGC AAGGGCGAGG 2041 AGCTGTTCAC CGGGGTGGTG CCCATCCTGG TCGAGCTGGA CGGCGACGTA AACGGCCACA 2101 AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG ATGCCACCTA CGGCAAGCTG ACCCTGAAGT 2161 TCATCTGCAC CACCGGCAAG CTGCCCGTGC CCTGGCCCAC CCTCGTGACC ACCCTGACCT 2221 ACGGCGTGCA GTGCTTCAGC CGCTACCCCG ACCACATGAA GCAGCACGAC TTCTTCAAGT 2281 CCGCCATGCC CGAAGGCTAC GTCCAGGAGC GCACCATCTT CTTCAAGGAC GACGGCAACT 2341 ACAAGACCCG CGCCGAGGTG AAGTTCGAGG GCGACACCCT GGTGAACCGC ATCGAGCTGA 2401 AGGGCATCGA CTTCAAGGAG GACGGCAACA TCCTGGGGCA CAAGCTGGAG TACAACTACA 2461 ACAGCCACAA CGTCTATATC ATGGCCGACA AGCAGAAGAA CGGCATCAAG GTGAACTTCA 2521 AGATCCGCCA CAACATCGAG GACGGCAGCG TGCAGCTCGC CGACCACTAC CAGCAGAACA 2581 CCCCCATCGG CGACGGCCCC GTGCTGCTGC CCGACAACCA CTACCTGAGC ACCCAGTCCG 2641 CCCTGAGCAA AGACCCCAAC GAGAAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG 2701 CCGCCGGGAT CACTCTCGGC ATGGACGAGC TGTACAAGTA AGATATCCGA TCCACCGGAT 2761 CTAGATAACT GATCATAATC AGCCATACCA CATTTGTAGA GGTTTTACTT GCTTTAAAAA 2821 ACCTCCCACA CCTCCCCCTG AACCTGAAAC ATAAAATGAA TGCAATTGTT GTTGTTAACT 2881 TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT TTCACAAATA 2941 AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT GTATCTTAAC 3001 GCGGTAACCA CGTGCGGACC CAATGGCCGC AGGAACCCCT AGTGATGGAG TTGGCCACTC 3061 CCTCTCTGCG CGCTCGCTCG CTCACTGAGG CCGGGCGACC AAAGGTCGCC CGACGCCCGG 3121 GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG CTGCCTGCAG GGGCGCCTGA 3181 TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATACGT CAAAGCAACC 3241 ATAGTACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT 3301 GACCGCTACA CTTGCCAGCG CCtTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT 3361 CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG 3421 ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TTGGGTGATG GTTCACGTAG 3481 TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA 3541 TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGGCT ATTCTTTTGA 3601 TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA 3661 ATTTAACGCG AATTTTAACA AAATATTAAC GTTTACAATT TTATGGTGCA CTCTCAGTAC 3721 AATCTGCTCT GATGCCGCAT AGTTAAGCCA GCCCCGACAC CCGCCAACAC CCGCTGACGC 3781 GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG 3841 GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGAC GAAAGGGCCT 3901 CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG 3961 TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC 4021 AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG 4081 GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG 4141 CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT 4201 GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT 4261 TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT 4321 ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA 4381 TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG 4441 AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC 4501 AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC 4561 TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC 4621 CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC 4681 TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT 4741 TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG 4801 TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT 4861 TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT 4921 AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA 4981 GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA 5041 TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA 5101 AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC 5161 AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT 5221 TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC 5281 GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT 5341 CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG 5401 ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC 5461 CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG 5521 CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC 5581 AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG 5641 GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT 5701 ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC 5761 TCACATGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.