pLV3-CMV-Lta-3×FLAG-EF1a-CopGF

pLV3-CMV-Lta-3×FLAG-EF1a-CopGFP-Neo plasmid

PVT94849

pLV3-CMV-Lta-3×FLAG-EF1a-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-Lta(mouse)-3×FLAG-EF1a-CopGFP-Neo
Alias: NM_010735.2;hlb382; LT; LT-alpha; LT-[a]; LTalpha; Ltx;LT[a]; TNF-beta; Tnfb; TNFSF1; Tnfsf1b; Tnlg1e
Gene lenght: 606bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-Lta-3×FLAG-EF1a-CopGFP-Neo plasmid Description

pLV3-CMV-Lta-3×FLAG-EF1a-CopGFP-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Lta-3×FLAG-EF1a-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgacact gctcggccgt ctccacctct tgagggtgct 2341 tggcacccct cctgtcttcc tcctggggct gctgctggcc ctgcctctag gggcccaggg 2401 actctctggt gtccgcttct ccgctgccag gacagcccat ccactccctc agaagcactt 2461 gacccatggc atcctgaaac ctgctgctca ccttgttggg taccccagca agcagaactc 2521 actgctctgg agagcaagca cggatcgtgc ctttctccga catggcttct ctttgagcaa 2581 caactccctc ctgatcccca ccagtggcct ctactttgtc tactcccagg tggttttctc 2641 tggagaaagc tgctccccca gggccattcc cactcccatc tacctggcac acgaggtcca 2701 gctcttttcc tcccaatacc ccttccatgt gcctctcctc agtgcgcaga agtctgtgta 2761 tccgggactt caaggaccgt gggtgcgctc aatgtaccag ggggctgtgt tcctgctcag 2821 taagggagac cagctgtcca cccacaccga cggcatctcc catctacact tcagccccag 2881 cagtgtattc tttggagcct ttgcactggg atccGACTAC AAAGACCATG ACGGTGATTA 2941 TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG TAgcggccgc Gaaggatctg 3001 cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt 3061 ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg tggcgcgggg taaactggga 3121 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag 3181 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacagctgaa 3241 gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc cctacctgag gccgccatcc 3301 acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt gcctcctgaa ctgcgtccgc 3361 cgtctaggta agtttaaagc tcaggtcgag accgggcctt tgtccggcgc tcccttggag 3421 cctacctaga ctcagccggc tctccacgct ttgcctgacc ctgcttgctc aactctacgt 3481 ctttgtttcg ttttctgttc tgcgccgtta cagatccaag ctgtgaccgg cgcctacgct 3541 agacgccacc atggagagcg acgagagcgg cctgcccgcc atggagatcg agtgccgcat 3601 caccggcacc ctgaacggcg tggagttcga gctggtgggc ggcggagagg gcacccccaa 3661 gcagggccgc atgaccaaca agatgaagag caccaaaggc gccctgacct tcagccccta 3721 cctgctgagc cacgtgatgg gctacggctt ctaccacttc ggcacctacc ccagcggcta 3781 cgagaacccc ttcctgcacg ccatcaacaa cggcggctac accaacaccc gcatcgagaa 3841 gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg ccggccgcgt 3901 gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga tcttcaccga 3961 caagatcatc cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg ataacgtgct 4021 ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc ggctactaca gcttcgtggt 4081 ggacagccac atgcacttca agagcgccat ccaccccagc atcctgcaga acgggggccc 4141 catgttcgcc ttccgccgcg tggaggagct gcacagcaac accgagctgg gcatcgtgga 4201 gtaccagcac gccttcaaga cccccatcgc cttcgccaga tcccgcgctc agtcgtccaa 4261 ttctgccgtg gacggcaccg ccggacccgg ctccaccgga tctcgcgagg gcagaggaag 4321 tcttctaaca tgcggtgacg tggaggagaa tcccggccct GCCACCatgg ctgaacaaga 4381 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 4441 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 4501 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 4561 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 4621 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 4681 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 4741 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 4801 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 4861 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt 4921 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 4981 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 5041 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 5101 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 5161 aGTCGACAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA 5221 TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC 5281 TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA 5341 GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC 5401 CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGACTT TCGCTTTCCC 5461 CCTCCCTATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGACAGGGGC 5521 TCGGCTGTTG GGCACTGACA ATTCCGTGGT GTTGTCGGGG AAATCATCGT CCTTTCCTTG 5581 GCTGCTCGCC TGTGTTGCCA CCTGGATTCT GCGCGGGACG TCCTTCTGCT ACGTCCCTTC 5641 GGCCCTCAAT CCAGCGGACC TTCCTTCCCG CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC 5701 GCGTCTTCGC CTTCGCCCTC AGACGAGTCG GATCTCCCTT TGGGCCGCCT CCCCGCggta 5761 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag 5821 gggggactgg aagggctaat tcactcccaa cgaaGataag atctgctttt tgcttgtact 5881 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca 5941 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg 6001 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc 6061 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca 6121 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc 6181 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc 6241 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg 6301 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 6361 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca 6421 gagacggccc aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc 6481 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat 6541 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc 6601 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg 6661 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 6721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 6781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 6841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 6901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 6961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 7021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 7081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 7141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 7201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 7261 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 7321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 7381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 7441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 7501 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 7561 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 7621 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 7681 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 7741 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 7801 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 7861 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 7921 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 7981 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 8041 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 8101 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 8161 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 8221 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 8281 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 8341 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 8401 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 8461 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 8521 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 8581 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 8641 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct 8701 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac 8761 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg 8821 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg 8881 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag 8941 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa 9001 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca 9061 gtgccaagct g

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.