pLV2-TRE3GS-SLFN13-TetOne-Puro

pLV2-TRE3GS-SLFN13-TetOne-Puro plasmid

PVT94304

pLV2-TRE3GS-SLFN13-TetOne-Puro plasmid Information

Plasmid name: pLV2-TRE3GS-SLFN13(human)-TetOne-Puro
Alias: NM_144682.6;hSLFN13; SLFN10
Gene lenght: 2694bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression,Tetracycline induction
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: TRE3GS
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: TRE3GS-F1
Reverse primer:
Copy number: High
Induction: Tetracycline induction

pLV2-TRE3GS-SLFN13-TetOne-Puro plasmid Description

pLV2-TRE3GS-SLFN13-TetOne-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-TRE3GS-SLFN13-TetOne-Puro plasmid Sequence

1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg 3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat 3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa 3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga 3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga 3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca 3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag 3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag 3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca 3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac 3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag 4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt 4081 tccagacgcg ttcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc 4141 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc 4201 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc 4261 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg 4321 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg 4381 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc 4441 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac 4501 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc 4561 tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg 4621 gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg 4681 tactcggtca tggtcgcgtt ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact 4741 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc 4801 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta 4861 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg 4921 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac 4981 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttcgcgcgtt 5041 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg 5101 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg 5161 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg 5221 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga 5281 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc 5341 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg 5401 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac 5461 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga 5521 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg 5581 gggcaggagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc 5641 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt 5701 ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc agagtttatg 5761 actttgctct tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg 5821 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg 5881 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc 5941 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc 6001 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg 6061 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga 6121 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg 6181 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc 6241 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg 6301 cgcaacccca accccgtgga attatcacct cgagtttact ccctatcagt gatagagaac 6361 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca 6421 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt 6481 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac 6541 gtatatccag tttactccct atcagtgata gagaacgtat aagctttgct tatgtaaacc 6601 agggcgccta taaaagagtg ctgatttttt gagtaaactt caattccaca acacttttgt 6661 cttataccaa ctttccgtac cacttcctac cctcgtaaag aattcGCCAC Catggaggca 6721 aatcactgct ccctgggtgt gtatccatct tacccagacc tggtcatcga tgtcggagaa 6781 gtgactctgg gagaagaaaa cagaaaaaag ctacagaaaa ctcagagaga ccaagagagg 6841 gcgagagtta tacgggccgc gtgtgcttta ttaaactcag gaggaggagt gattcagatg 6901 gaaatggcca acagggatga gcgtcccaca gagatgggac tggatttaga agaatccttg 6961 agaaagctta ttcagtatcc atatttgcag gctttctttg agactaagca acacggaagg 7021 tgtttttata tttttgttaa atcttggagt ggtgatcctt tccttaaaga tggttctttc 7081 aattcccgca tttgcagcct tagttcttca ttatactgta gatctggcac ctctgtgctt 7141 cacatgaatt caagacaggc attcgatttc ctgaagacca aggaaagaca gtccaaatat 7201 aatctgatta atgaagggtc tccacctagt aaaattatga aagctgtata ccagaacata 7261 tctgagtcaa atcctgcata tgaagttttc caaactgaca ctattgaata tggtgaaatc 7321 ctatcttttc ctgagtctcc atccatagag tttaaacagt tctctacaaa acatatccaa 7381 caatatgtag aaaatataat tccagagtac atctctgcat ttgcaaacac tgagggaggc 7441 tatcttttta ttggagtgga tgataagagt aggaaagtcc tgggatgtgc caaagaacag 7501 gttgaccctg actctttgaa aaatgtaatt gcaagagcaa tttctaagtt gcccattgtt 7561 catttttgct cttcaaaacc tcgggtagag tacagcacca aaatcgtaga agtgttttgt 7621 gggaaagagt tgtatggcta tctctgtgtg attaaagtga aggcattctg ttgtgtggtg 7681 ttctcggaag ctcccaagtc atggatggtg agggagaagt acatccgccc cttgacaact 7741 gaggaatggg tagagaaaat gatggacgca gatccagagt ttcctccaga ctttgctgag 7801 gcctttgagt ctcagttgag tctatctgac agtccttcac tttgcagacc agtgtattct 7861 aagaaaggtc tggaacacaa agctgatcta caacaacatt tatttccagt tccaccagga 7921 catttggaat gtactccaga gtccctctgg aaggagctgt ctttacagca tgaaggacta 7981 aaggagttaa tacacaagca aatgcgacct ttctcccagg gaattgtgat cctctctaga 8041 agctgggctg tggacctgaa cttgcaggag aagccaggag tcatctgtga tgctctgctg 8101 atagcacaga acagcacccc cattctctac accattctca gggagcagga tgcagagggc 8161 caggactact gcactcgcac cgcctttact ttgaagcaga agctagtgaa catggggggc 8221 tacaccggga aggtgtgtgt cagggccaag gtcctctgcc tgagtcctga gagcagcgca 8281 gaggccttgg aggctgcagt gtctccgatg gattaccctg cgtcctatag ccttgcaggc 8341 acccagcaca tggaagccct gctgcagtcc ctcgtgattg tcttactcgg cttcaggtct 8401 ctcttgagtg accagctcgg ctgtgaggtt ttaaatctgc tcacagccca gcagtatgag 8461 atattctcca gaagcctccg caagaacaga gagttgtttg tccacggctt acctggctca 8521 gggaagacca tcatggccat gaagatcatg gagaagatca ggaatgtgtt tcactgtgag 8581 gcacacagaa ttctctacgt ttgtgaaaac cagcctctga ggaactttat cagtgataga 8641 aatatctgcc gagcagagac ccgggaaact ttcctaagag aaaaatttga acacattcaa 8701 cacatcgtca ttgacgaagc tcagaatttc cgtactgaag atggggactg gtataggaag 8761 gcaaaaacca tcactcagag agaaaaggat tgtccaggag ttctctggat ctttctggac 8821 tactttcaga ccagtcactt gggtcacagt ggccttcccc ctctctcagc acagtatcca 8881 agagaagagc tcaccagagt agttcgcaat gcagatgaaa tagccgagta catacaacaa 8941 gaaatgcaac taattataga aaatcctcca attaatatcc cccatgggta tctggcaatt 9001 ctcagtgaag ctaaatgggt tccaggtgtt ccaggcaaca caaagattat taaaaacttt 9061 actttggagc aaatagtgac ctatgtggca gacacctgca ggtgcttctt tgaaaggggc 9121 tattctccaa aggatgttgc tgtgcttgtc agcaccgtga cagaagtgga gcagtatcag 9181 tctaagctct tgaaagcaat gaggaagaaa atggtggtgc agctcagtga tgcatgtgat 9241 atgttgggtg tgcacattgt gttggacagt gtccggcgat tctcaggcct ggaaaggagc 9301 atagtgtttg ggatccatcc aaggacagct gacccagcta tcttacccaa tattctgatc 9361 tgtctggctt ccagggcaaa acagcaccta tatatttttc tgtgaggatc cagaccacct 9421 cccctgcgag ctaagctgga cagccaatga cgggtaagag agtgacattt ttcactaacc 9481 taagacagga gggccgtcag agctactgcc taatccaaag acgggtaaaa gtgataaaaa 9541 tgtatcactc caacctaaga caggcgcagc ttccgaggga tttgagatcc agacatgata 9601 agatacattg atgagtttgg acaaaccaaa actagaatgc agtgaaaaaa atgccttatt 9661 tgtgaaattt gtgatgctat tgccttattt gtaaccatta taagctgcaa taaacaagtt 9721 aagtcgataa actggatctc tgctgtccct gtaataaacc cgaaaatttt gaatttttgt 9781 aatttgtttt tgtagttctt tagtttgtat gtctgttgct attatgtcta ctattctttc 9841 ccctgcactg taccccccaa tccccccttt tcttttaaag gcgataccgt cgagatccgt 9901 tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg 9961 gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct 10021 gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc 10081 ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc 10141 acaaacttgc ccatttatct aattccaata attcttgttc attcttttct tgctggtttt 10201 gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact 10261 ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc 10321 aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt 10381 gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga 10441 ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct 10501 gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca 10561 tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt 10621 tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt 10681 ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag 10741 gtctgaagat cagcggccgg ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc 10801 ctctatcttg tctaaagctt ccttggtgtc ttttatctct atcctttgat gcacacaata 10861 gagggttgct actgtattat ataatgatct aagttcttct gatcctgtct gaagggatgg 10921 ttgtagctgt cccagtattt gtctacagcc ttctgatgtt tctaacaggc caggattaac 10981 tgcgaatcgt tctagctccc tgcttgccca tactatatgt tttaatttat attttttctt 11041 tccccctggc cttaaccgaa ttttttccca tcgcgatcta attctccccc gcttaatact 11101 gacgctctcg cacccatctc tctccttcta gcctccgcta gtcaaaattt ttggcgtact 11161 caccagtcgc cgcccctcgc ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg 11221 agagagctcc tctggtttcc ctttcgcttt caagtccctg ttcgggcgcc actgctagag 11281 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga 11341 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc 11401 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacag 11461 gcaaaaagca gctgcttata tgcaggatct gagggctcgc cactccccag tcccgcccag 11521 gccacgcctc cctggaaagt ccccagcgga aagtcccttg tagcaagctc gatatcagca 11581 gttcttgaag tactccggat gcagctctcg ggccacgtga tgaaatgcta ggcggctgtc 11641 aaacctccac tctaacactt ctctctccgg gtcatccatc ccatgcaggc tcacagggtg 11701 taacaagctg gtgttctctc ctttattggc ctcttctacc ttatctggct caactggtac 11761 tagcttgtag caccatccaa aggtcagtgg atatctgacc cctggccctg gtgtgtagtt 11821 ctgctaatca gggaagtagc cttgtgtgtg gtagatccac agatcaagga tatcttgtct 11881 tctttgggag tgaattagcc cttcca

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.