pLV3-CMV-OSR2-3×FLAG-EF1a-CopG

pLV3-CMV-OSR2-3×FLAG-EF1a-CopGFP-Neo plasmid

PVT95552

pLV3-CMV-OSR2-3×FLAG-EF1a-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-OSR2(human)-3×FLAG-EF1a-CopGFP-Neo
Alias: NM_001286841.2
Gene lenght: 1299bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-OSR2-3×FLAG-EF1a-CopGFP-Neo plasmid Description

pLV3-CMV-OSR2-3×FLAG-EF1a-CopGFP-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-OSR2-3×FLAG-EF1a-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA Ccatggggct gagggcagag gaaggaaaaa gaaaacctcc 2341 gaggtcagtg cggggcgagg tgagcccctc ccagggccct ctggcccagg aggatgaagc 2401 gcgccggctt cgctcttgca cgccggcttg ccatccgggt aagcgcggga aaggcggcca 2461 cagggcgcgg cggcagcgca gcgcgtggga tctcacgacc catccgttaa cccaccgttc 2521 ccaggagctc cgaggcgcag cggcgacaga gggctttctc tacgtgctgt tgtctcactg 2581 ggtttttgtc ggagccccac gccctccggc ctctgattcc tggaagaaag ggttggtccc 2641 ctcagcaccc ccagcatccc ggaaaatggg gagcaaggct ctgccagcgc ccatcccgct 2701 ccacccgtcg ctgcagctca ccaattactc cttcctgcag gccgtgaaca ccttcccggc 2761 cacggtggac cacctgcagg gcctgtacgg tctcagcgcg gtacagacca tgcacatgaa 2821 ccactggacg ctggggtatc ccaatgtgca cgagatcacc cgctccacca tcacggagat 2881 ggcggcggcg cagggcctcg tggacgcgcg cttccccttc ccggccctgc cttttaccac 2941 ccacctattc caccccaagc agggggccat tgcccacgtc ctcccagccc tgcacaagga 3001 ccggccccgt tttgactttg ccaatttggc ggtggctgcc acgcaagagg atccgcctaa 3061 gatgggagac ctgagcaagc tgagcccagg actgggtagc cccatctcgg gcctcagtaa 3121 attgactccg gacagaaagc cctctcgagg aaggttgccc tccaaaacga aaaaagagtt 3181 tatctgcaag ttttgcggca gacactttac caaatcctac aatttgctca tccatgagag 3241 gacccacacg gacgagaggc cgtacacgtg tgacatctgc cacaaggcct tccggaggca 3301 agatcacctg cgggatcaca gatacatcca ttccaaagaa aaacccttca aatgtcagga 3361 gtgtgggaaa ggattttgtc agtctagaac tctagcagtt cacaaaactt tacacatgca 3421 ggaatctcca cacaaatgtc ccacatgtgg aagaaccttt aatcagagaa gtaatctgaa 3481 aactcacctt ctcacccata cagacatcaa gccctacagc tgcgagcagt gcggcaaagt 3541 gttcaggcga aactgtgatc tgcggcggca cagcctgact cacaccccgc ggcaggactt 3601 cggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA 3661 TGACGATGAC AAGTAgcggc cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc 3721 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg 3781 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 3841 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 3901 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc 3961 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc 4021 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc 4081 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac 4141 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg 4201 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag 4261 cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt 4321 cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa 4381 gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg 4441 cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa 4501 caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt 4561 gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac 4621 cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt 4681 ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag 4741 cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc 4801 catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga 4861 gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat 4921 cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc 4981 cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga 5041 gaatcccggc cctGCCACCa tggctgaaca agatggattg cacgcaggtt ctccggccgc 5101 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 5161 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 5221 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg 5281 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 5341 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 5401 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 5461 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 5521 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 5581 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 5641 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 5701 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 5761 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 5821 cgccttctat cgccttcttg acgagttctt ctgaGTCGAC AATCAACCTC TGGATTACAA 5881 AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA 5941 CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC 6001 CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG 6061 TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC 6121 CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT 6181 CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT 6241 GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC GCCTGTGTTG CCACCTGGAT 6301 TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC 6361 CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG 6421 TCGGATCTCC CTTTGGGCCG CCTCCCCGCg gtacctttaa gaccaatgac ttacaaggca 6481 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 6541 caacgaaGat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga 6601 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 6661 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 6721 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 6781 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca 6841 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 6901 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc 6961 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 7021 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 7081 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt 7141 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 7201 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 7261 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 7321 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 7381 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 7441 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 7501 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 7561 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 7621 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 7681 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 7741 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 7801 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 7861 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 7921 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 7981 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 8041 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 8101 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 8161 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 8221 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 8281 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 8341 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 8401 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 8461 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 8521 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 8581 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 8641 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 8701 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 8761 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 8821 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 8881 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 8941 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 9001 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 9061 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 9121 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 9181 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 9241 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag 9301 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 9361 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 9421 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 9481 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 9541 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 9601 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 9661 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 9721 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.