pLV3-CMV-LAP3-3×FLAG-mCherry-P

pLV3-CMV-LAP3-3×FLAG-mCherry-Puro plasmid

PVT94843

pLV3-CMV-LAP3-3×FLAG-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-LAP3(human)-3×FLAG-mCherry-Puro
Alias: NM_015907.3;HEL-S-106; LAP; LAPEP; PEPS
Gene lenght: 1557bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-LAP3-3×FLAG-mCherry-Puro plasmid Description

pLV3-CMV-LAP3-3×FLAG-mCherry-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-LAP3-3×FLAG-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgttctt gctgcctctt ccggctgcgg ggcgagtagt 2341 cgtccgacgt ctggccgtga gacgtttcgg gagccggagt ctctccaccg cagacatgac 2401 gaagggcctt gttttaggaa tctattccaa agaaaaagaa gatgatgtgc cacagttcac 2461 aagtgcagga gagaattttg ataaattgtt agctggaaag ctgagagaga ctttgaacat 2521 atctggacca cctctgaagg cagggaagac tcgaaccttt tatggtctgc atcaggactt 2581 ccccagcgtg gtgctagttg gcctcggcaa aaaggcagct ggaatcgacg aacaggaaaa 2641 ctggcatgaa ggcaaagaaa acatcagagc tgctgttgca gcggggtgca ggcagattca 2701 agacctggag ctctcgtctg tggaggtgga tccctgtgga gacgctcagg ctgctgcgga 2761 gggagcggtg cttggtctct atgaatacga tgacctaaag caaaaaaaga agatggctgt 2821 gtcggcaaag ctctatggaa gtggggatca ggaggcctgg cagaaaggag tcctgtttgc 2881 ttctgggcag aacttggcac gccaattgat ggagacgcca gccaatgaga tgacgccaac 2941 cagatttgct gaaattattg agaagaatct caaaagtgct agtagtaaaa ccgaggtcca 3001 tatcagaccc aagtcttgga ttgaggaaca ggcaatggga tcattcctca gtgtggccaa 3061 aggatctgac gagcccccag tcttcttgga aattcactac aaaggcagcc ccaatgcaaa 3121 cgaaccaccc ctggtgtttg ttgggaaagg aattaccttt gacagtggtg gtatctccat 3181 caaggcttct gcaaatatgg acctcatgag ggctgacatg ggaggagctg caactatatg 3241 ctcagccatc gtgtctgctg caaagcttaa tttgcccatt aatattatag gtctggcccc 3301 tctttgtgaa aatatgccca gcggcaaggc caacaagccg ggggatgttg ttagagccaa 3361 aaacgggaag accatccagg ttgataacac tgatgctgag gggaggctca tactggctga 3421 tgcgctctgt tacgcacaca cgtttaaccc gaaggtcatc ctcaatgccg ccaccttaac 3481 aggtgccatg gatgtagctt tgggatcagg tgccactggg gtctttacca attcatcctg 3541 gctctggaac aaactcttcg aggccagcat tgaaacaggg gaccgtgtct ggaggatgcc 3601 tctcttcgaa cattatacaa gacaggttgt agattgccag cttgctgatg ttaacaacat 3661 tggaaaatac agatctgcag gagcatgtac agctgcagca ttcctgaaag aattcgtaac 3721 tcatcctaag tgggcacatt tagacatagc aggcgtgatg accaacaaag atgaagttcc 3781 ctatctacgg aaaggcatga ctgggaggcc cacaaggact ctcattgagt tcttacttcg 3841 tttcagtcaa gacaatgctg gatccGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA 3901 TGACATCGAC TACAAGGATG ACGATGACAA Gtagcggccg cGaaggatct gcgatcgctc 3961 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 4021 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 4081 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 4141 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg 4201 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt 4261 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt 4321 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag 4381 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc 4441 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac 4501 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa 4561 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg 4621 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc 4681 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca 4741 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg 4801 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga 4861 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt 4921 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg 4981 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc 5041 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt 5101 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga 5161 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg gatctcgcga 5221 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga 5281 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct 5341 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat 5401 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa 5461 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga 5521 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct 5581 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg 5641 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt 5701 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc 5761 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga 5821 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc 5881 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac 5941 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt 6001 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt 6061 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg 6121 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac 6181 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac 6241 tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc tcgcctgtgt 6301 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc 6361 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg 6421 ccctcagacg agtcggatct ccctttggcc gcctccccgc ctggtacctt taagaccaat 6481 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 6541 gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt ctctctggtt 6601 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 6661 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 6721 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 6781 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 6841 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 6901 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 6961 tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca ttctccgccc 7021 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 7081 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga cggcccaaat 7141 tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 7201 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 7261 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 7321 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 7381 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 7441 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 7501 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 7561 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 7621 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 7681 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 7741 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 7801 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 7861 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 7921 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 7981 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 8041 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 8101 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 8161 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 8221 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 8281 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 8341 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 8401 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 8461 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 8521 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 8581 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 8641 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 8701 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 8761 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 8821 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 8881 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 8941 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 9001 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 9061 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 9121 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 9181 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 9241 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 9301 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 9361 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 9421 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc 9481 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt 9541 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac 9601 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg 9661 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg 9721 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.