pLV3-CMV-CDH1-3×FLAG-CopGFP-Pu

pLV3-CMV-CDH1-3×FLAG-CopGFP-Puro plasmid

PVT95893

pLV3-CMV-CDH1-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-CDH1(human)-3×FLAG-CopGFP-Puro
Alias: NM_001317184.2;Arc-1; BCDS1; CD324; CDHE; ECAD; LCAM; UVO
Gene lenght: 2463bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CDH1-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-CDH1-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-CDH1-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg gcccttggag ccgcagcctc 2341 tcggcgctgc tgctgctgct gcaggtctcc tcttggctct gccaggagcc ggagccctgc 2401 caccctggct ttgacgccga gagctacacg ttcacggtgc cccggcgcca cctggagaga 2461 ggccgcgtcc tgggcagagt gaattttgaa gattgcaccg gtcgacaaag gacagcctat 2521 ttttccctcg acacccgatt caaagtgggc acagatggtg tgattacagt caaaaggcct 2581 ctacggtttc ataacccaca gatccatttc ttggtctacg cctgggactc cacctacaga 2641 aagttttcca ccaaagtcac gctgaataca gtggggcacc accaccgccc cccgccccat 2701 caggcctccg tttctggaat ccaagcagaa ttgctcacat ttcccaactc ctctcctggc 2761 ctcagaagac agaagagaga ctgggttatt cctcccatca gctgcccaga aaatgaaaaa 2821 ggcccatttc ctaaaaacct ggttcagatc aaatccaaca aagacaaaga aggcaaggtt 2881 ttctacagca tcactggcca aggagctgac acaccccctg ttggtgtctt tattattgaa 2941 agagaaacag gatggctgaa ggtgacagag cctctggata gagaacgcat tgccacatac 3001 actctcttct ctcacgctgt gtcatccaac gggaatgcag ttgaggatcc aatggagatt 3061 ttgatcacgg taaccgatca gaatgacaac aagcccgaat tcacccagga ggtctttaag 3121 gggtctgtca tggaaggtgc tcttccagga acctctgtga tggaggtcac agccacagac 3181 gcggacgatg atgtgaacac ctacaatgcc gccatcgctt acaccatcct cagccaagat 3241 cctgagctcc ctgacaaaaa tatgttcacc attaacagga acacaggagt catcagtgtg 3301 gtcaccactg ggctggaccg agagagtttc cctacgtata ccctggtggt tcaagctgct 3361 gaccttcaag gtgaggggtt aagcacaaca gcaacagctg tgatcacagt cactgacacc 3421 aacgataatc ctccgatctt caatcccacc acgggcttgg attttgaggc caagcagcag 3481 tacattctac acgtagcagt gacgaatgtg gtaccttttg aggtctctct caccacctcc 3541 acagccaccg tcaccgtgga tgtgctggat gtgaatgaag cccccatctt tgtgcctcct 3601 gaaaagagag tggaagtgtc cgaggacttt ggcgtgggcc aggaaatcac atcctacact 3661 gcccaggagc cagacacatt tatggaacag aaaataacat atcggatttg gagagacact 3721 gccaactggc tggagattaa tccggacact ggtgccattt ccactcgggc tgagctggac 3781 agggaggatt ttgagcacgt gaagaacagc acgtacacag ccctaatcat agctacagac 3841 aatggttctc cagttgctac tggaacaggg acacttctgc tgatcctgtc tgatgtgaat 3901 gacaacgccc ccataccaga acctcgaact atattcttct gtgagaggaa tccaaagcct 3961 caggtcataa acatcattga tgcagacctt cctcccaata catctccctt cacagcagaa 4021 ctaacacacg gggcgagtgc caactggacc attcagtaca acgacccaac ccaagaatct 4081 atcattttga agccaaagat ggccttagag gtgggtgact acaaaatcaa tctcaagctc 4141 atggataacc agaataaaga ccaagtgacc accttagagg tcagcgtgtg tgactgtgaa 4201 ggggccgctg gcgtctgtag gaaggcacag cctgtcgaag caggattgca aattcctgcc 4261 attctgggga ttcttggagg aattcttgct ttgctaattc tgattctgct gctcttgctg 4321 tttcttcgga ggagagcggt ggtcaaagag cccttactgc ccccagagga tgacacccgg 4381 gacaacgttt attactatga tgaagaagga ggcggagaag aggaccagga ctttgacttg 4441 agccagctgc acaggggcct ggacgctcgg cctgaagtga ctcgtaacga cgttgcacca 4501 accctcatga gtgtcccccg gtatcttccc cgccctgcca atcccgatga aattggaaat 4561 tttattgatg aaaatctgaa agcggctgat actgacccca cagccccgcc ttatgattct 4621 ctgctcgtgt ttgactatga aggaagcggt tccgaagctg ctagtctgag ctccctgaac 4681 tcctcagagt cagacaaaga ccaggactat gactacttga acgaatgggg caatcgcttc 4741 aagaagctgg ctgacatgta cggaggcggc gaggacgacg gatccGACTA CAAAGACCAT 4801 GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA Gtagcggccg 4861 cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc gcccacagtc 4921 cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg 4981 gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa 5041 ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga 5101 acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg ccctacctga 5161 ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga 5221 actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg 5281 ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac cctgcttgct 5341 caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg 5401 gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc catggagatc 5461 gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg cggcggagag 5521 ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg cgccctgacc 5581 ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt cggcacctac 5641 cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta caccaacacc 5701 cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag 5761 gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg 5821 atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca ccccatgggc 5881 gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg cggctactac 5941 agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag catcctgcag 6001 aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa caccgagctg 6061 ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag atcccgcgct 6121 cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg atctcgcgag 6181 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tatgaccgag 6241 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc 6301 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc 6361 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag 6421 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa 6481 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg 6541 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg 6601 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc 6661 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc 6721 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag 6781 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg aaatcaacct 6841 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg 6901 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc 6961 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt 7021 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc 7081 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg 7141 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact 7201 gacaattccg tggtgttgtc ggggaaATCA TCGTCCTTTC CTtggctgct cgcctgtgtt 7261 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg 7321 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct Tcgccttcgc 7381 cctcagacga gtcggatctc cctttggGcc gcctccccgc ctggtacctt taagaccaat 7441 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg 7501 gctaattcac tcccaacgaa Gataagatct gctttttgct tgtactgggt ctctctggtt 7561 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca 7621 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa 7681 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg 7741 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa 7801 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 7861 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 7921 tcatgtctgg ctctagctaT CCCGCCCCTA ACTCCGCCCA tcccgcccct aactccgccc 7981 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 8041 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctagac 8101 ttttgcagag accaaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc 8161 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 8221 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 8281 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 8341 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 8401 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 8461 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 8521 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 8581 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 8641 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 8701 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 8761 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 8821 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 8881 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 8941 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 9001 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 9061 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 9121 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 9181 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 9241 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 9301 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 9361 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 9421 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 9481 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 9541 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 9601 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 9661 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 9721 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 9781 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 9841 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 9901 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 9961 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 10021 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 10081 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 10141 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 10201 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 10261 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 10321 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct 10381 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 10441 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc 10501 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 10561 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga 10621 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc 10681 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc 10741 cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.