pLV3-CMV-3×FLAG-KAT8-mCherry-P

pLV3-CMV-3×FLAG-KAT8-mCherry-Puro plasmid

PVT94848

pLV3-CMV-3×FLAG-KAT8-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-3×FLAG-KAT8(human)-mCherry-Puro
Alias: NM_032188.3;hMOF; LIGOWS; MOF; MYST1; ZC2HC8
Gene lenght: 1377bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-3×FLAG-KAT8-mCherry-Puro plasmid Description

pLV3-CMV-3×FLAG-KAT8-mCherry-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-3×FLAG-KAT8-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggacta caaagaccat gacggtgatt ataaagatca 2341 tgacatcgac tacaaggatg acgatgacaa gctcgagatg gcggcacagg gagctgctgc 2401 ggcggttgcg gcggggactt caggggtcgc gggggagggc gagcccgggc ccggggagaa 2461 tgcggccgct gaggggaccg ccccatcccc gggccgcgtc tctccgccga ccccggcgcg 2521 cggcgagccg gaagtcacgg tggagatcgg agaaacgtac ctgtgccggc gaccggatag 2581 cacctggcat tctgctgaag tgatccagtc tcgagtgaac gaccaggagg gccgagagga 2641 attctatgta cactacgtgg gctttaaccg gcggctggac gagtgggtag acaagaaccg 2701 gctggcgctg accaagacag tgaaggatgc tgtacagaag aactcagaga agtacctgag 2761 cgagctcgca gagcagcctg agcgcaagat cactcgcaac caaaagcgca agcatgatga 2821 gatcaaccat gtgcagaaga cttatgcaga gatggacccc accacagcag ccttggagaa 2881 ggagcatgag gcgatcacca aggtgaagta tgtggacaag atccacatcg ggaactacga 2941 aattgatgcc tggtatttct caccattccc cgaagactat gggaaacagc ccaagctctg 3001 gctctgcgag tactgcctca agtacatgaa atatgagaag agctaccgct tccacttggg 3061 tcagtgccag tggcggcagc cccccgggaa agagatctac cgcaagagca acatctccgt 3121 gtacgaagtt gatggcaaag accataagat ttactgtcag aacctgtgtc tgctggccaa 3181 gcttttcctg gaccataaga cactgtactt tgacgtggag ccgttcgtct tttacatcct 3241 gactgaggtg gaccggcagg gggcccacat tgttggctac ttctccaagg agaaggagtc 3301 cccggatgga aacaatgtgg cctgcatcct gaccttgccc ccctaccaac gccgcggcta 3361 cgggaagttc ctcatcgctt tcagttatga gctctccaag ctggagagca cagtcggctc 3421 cccggagaag ccactgtctg acctgggcaa gctcagctac cgcagctact ggtcctgggt 3481 gctgctagag atcctgcggg acttccgggg cacactgtcc atcaaggacc tcagccagat 3541 gaccagtatc acccaaaatg acatcatcag taccctgcaa tccctcaata tggtcaagta 3601 ctggaagggc cagcacgtga tctgtgtcac acccaagctg gtggaggagc acctcaaaag 3661 tgcccagtat aagaaaccac ccatcacagt ggactccgtc tgcctcaagt gggcaccccc 3721 caagcacaag caagtcaagc tctccaagaa gtgaggatcc gcggccgcGa aggatctgcg 3781 atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 3841 ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa 3901 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 3961 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc 4021 ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac 4081 gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg 4141 tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc 4201 tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct 4261 ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag 4321 acgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc 4381 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg 4441 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc 4501 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg 4561 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt 4621 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc 4681 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg 4741 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg 4801 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact 4861 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct 4921 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac 4981 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagggat 5041 ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggcccta 5101 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac 5161 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc 5221 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca 5281 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga 5341 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt 5401 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc 5461 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca 5521 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg 5581 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg 5641 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgaa 5701 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc 5761 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta 5821 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt 5881 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg 5941 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta 6001 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt 6061 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg 6121 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca 6181 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtctcc 6241 gccttcgccc tcagacgagt cggatctccc tttggccgcc tccccgcctg gtacctttaa 6301 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac 6361 tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt actgggtctc 6421 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 6481 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 6541 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta 6601 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg 6661 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 6721 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 6781 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt ccgcccattc 6841 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc 6901 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacgg 6961 cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat 7021 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 7081 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 7141 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 7201 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 7261 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 7321 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 7381 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 7441 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 7501 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 7561 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 7621 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 7681 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 7741 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 7801 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 7861 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 7921 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 7981 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 8041 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 8101 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 8161 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 8221 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 8281 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 8341 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 8401 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 8461 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 8521 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 8581 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 8641 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 8701 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 8761 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 8821 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 8881 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 8941 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 9001 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 9061 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 9121 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 9181 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 9241 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 9301 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 9361 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 9421 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 9481 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 9541 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 9601 gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.