pLV3-EF1a-HIVEP3-Puro plasmid

pLV3-EF1a-HIVEP3-Puro plasmid

PVT96572

pLV3-EF1a-HIVEP3-Puro plasmid Information

Plasmid name: pLV3-EF1a-HIVEP3(human)-Puro
Alias: NM_024503.5;KBP-1; KBP1; KRC; Schnurri-3; SHN3; ZAS3;ZNF40C;Inducing agents should be added during extraction
Gene lenght: 7218bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): EPI300
Growth Temperature: 30°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: Low
Induction:

pLV3-EF1a-HIVEP3-Puro plasmid Description

pLV3-EF1a-HIVEP3-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-EF1a-HIVEP3-Puro plasmid Sequence

1 ttttggattg aagccaatat gataatgagg gggtggagtt tgtgacgtgg cgcggggcgt 61 gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg atgttgcaag tgtggcggaa 121 cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg tgtgcgccgg tgtacacagg 181 aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt aaatttgggc gtaaccgagt 241 aagatttggc cattttcgcg ggaaaactga ataagaggaa gtgaaatctg aataattttg 301 tgttactcat agcgcgtaat acggcagacc tcagcgctag attattgaag catttatcag 361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 421 gttccgcgca catttccccg aaaagtgcca cctgacgtta actataacgg tcctaaggta 481 gcgaaaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag 541 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg 601 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact 661 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct 721 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 781 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 841 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 901 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 961 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1021 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1081 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1141 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1201 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1261 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1321 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1381 aaagtaagac caccgcacag caagcccgct gatcttcaga cctggaggag gagatatgag 1441 ggacattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta 1501 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga 1561 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg 1621 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg 1681 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc 1741 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg 1801 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat 1861 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac 1921 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat 1981 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca 2041 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga 2101 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg 2161 tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa 2221 ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg acggtatcga 2281 aagcttggga ttcgaattta aaagaaaagg ggggattggg gggtacagtg caggggaaag 2341 aatagtagac ataatagcaa cagacataca aactaaagaa ctacaaaaac aaattacaaa 2401 aattcaaaat tttcgggttt ttcgaaccta gggagtggga attggctccg gtgcccgtca 2461 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 2521 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 2581 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 2641 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg 2701 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca ctggctgcag 2761 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc 2821 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc 2881 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc 2941 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat 3001 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc 3061 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc 3121 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt 3181 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga 3241 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag 3301 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca 3361 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg 3421 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag 3481 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc 3541 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt 3601 cttccatttc aggtgtcgtg aggaatttcg actgctagct ggtacctgaa ttgagtactt 3661 acgtaggtac cccagtgtgg tggcctgcag gtgaattcGC CACCatggat cctgaacaaa 3721 gtgtcaaggg caccaagaag gctgagggaa gtccccggaa gcggctgacc aaaggagagg 3781 ccattcagac cagtgtttct tccagcgtcc catacccagg cagcggcaca gctgccaccc 3841 aagagagccc cgcccaagag ctcttagccc cgcagccctt cccgggcccc tcatcagttc 3901 ttagggaagg ctctcaggag aaaacgggcc agcagcagaa gccccccaaa aggcccccca 3961 tcgaagcatc cgtccacatc tcacagcttc cgcagcaccc tctgacacca gcattcatgt 4021 cgcctggcaa acctgagcat ctcctggagg ggtccacatg gcaactggtt gaccccatga 4081 gacctggacc ctctggctcc ttcgtggccc ctgggctcca tcctcagagc cagctccttc 4141 cttcccacgc ttccatcatt ccccccgagg accttcctgg agtccccaaa gtcttcgtgc 4201 ctcgtccttc ccaggtctcc ttgaagccca cagaagaggc acacaagaag gagaggaagc 4261 cccagaagcc aggcaagtac atctgccagt actgcagccg gccctgtgcc aagcccagcg 4321 tgctccagaa gcacattcgc tcacacacag gtgagaggcc ctacccctgc ggcccctgtg 4381 gcttctcctt caagaccaag agtaatctct acaagcacag gaagtcccat gcccaccgca 4441 tcaaagcagg cctggcctca ggcatgggtg gcgagatgta cccacatggg ctggagatgg 4501 agcggatccc tggggaagag tttgaggagc ccactgaggg agaaagcaca gattctgaag 4561 aggagactag tgccacctct ggtcaccctg cagagctctc cccaagaccc aagcagcccc 4621 ttctctccag cgggctatac agctctggga gccacagttc cagccacgaa cgctgttccc 4681 tgtcccagtc cagcacagcc cagtcactcg aagacccccc tccatttgtg gaaccctcat 4741 ctgagcaccc cctgagccat aaacctgaag acacccacac gattaagcag aagctggccc 4801 tccgcttaag cgagaggaag aaggtgatcg atgagcaggc gtttctgagc ccaggcagca 4861 aagggagtac tgagtctggg tatttctctc gctccgagag tgcagagcag caggtcagcc 4921 ccccaaacac caacgccaag tcctacgctg agatcatctt tggcaagtgt gggcgaatag 4981 gacagcggac cgccatgctg acagccacct ccacccagcc cctcctgccc ctgtccaccg 5041 aagacaagcc cagcctggtg cctttgtctg taccccggac gcaggtgatc gagcacatca 5101 cgaagctcat caccatcaac gaggccgtgg tggacaccag cgagatcgac agcgtgaagc 5161 caaggcggag ctcactgtcc aggcgcagca gcatggagtc cccaaaatcc agcctctacc 5221 gggagcccct gtcatcccac agtgagaaaa ccaagcctga acaatcactg ctgagcctcc 5281 agcacccgcc cagtaccgcc ccccctgtgc ctctcctgag aagccactca atgccttctg 5341 ccgcctgcac tatcagcacc ccccaccacc ccttccgagg tagctactcc ttcgatgacc 5401 atatcaccga ctccgaagcc ctgagccaca gcagtcacgt gtttacctcc cacccccgga 5461 tgctgaagcg ccagccggca atcgaattac ctttgggagg ggaatacagt tctgaggagc 5521 ctggcccaag cagcaaagac acagcctcca agccctcgga cgaagtggaa cccaaggaaa 5581 gcgagcttac caaaaagacc aagaagggtt tgaaaacaaa aggggtgatc tacgaatgta 5641 acatatgtgg tgctcggtac aagaaaaggg ataactacga agcccacaaa aaatactact 5701 gctcagagct tcagatcgca aagcccatct ctgcaggcac ccacacatct ccagaagctg 5761 aaaagagtca gattgagcat gagccgtggt cccaaatgat gcattacaaa ctgggaacca 5821 ccctggaact cactccactg aggaagagga ggaaagagaa gagccttggg gacgaggaag 5881 agccacctgc ctttgagtcc acaaaaagtc agtttggcag ccccgggcca tctgatgctg 5941 ctcggaacct tcccctggag tccaccaagt caccagcaga accaagtaaa tcagtgccct 6001 ccttggaggg acccacgggc ttccagccaa ggactcccaa gccagggtcc ggttcagaat 6061 cagggaagga gaggagaaca acgtccaaag aaatttctgt catccagcac accagctcct 6121 ttgagaaatc tgattctctc gagcagccga gtggcttgga aggggaagac aaacctctgg 6181 cccagttccc atcaccccca cctgccccac acggacgctc tgctcactcc ctgcagccta 6241 agttggtccg ccagcccaac attcaggttc ctgagatcct agtaactgag gagcctgacc 6301 ggccggacac agagccagag ccgcccccta aggaacctga gaagactgag gagttccaat 6361 ggccccagcg cagccagaca cttgcccagc tcccagctga gaagctgcca cccaaaaaga 6421 agaggttgcg cctggcagag atggcccaat catcagggga gtccagcttc gagtcctctg 6481 tgcctctgtc tcgcagcccg agccaggaaa gcaatgtctc tttgagtggg tccagccgct 6541 cagcctcgtt tgagagggat gaccatggga aagccgaggc ccccagtccc tcatctgaca 6601 tgcgccccaa acccctgggc acccacatgt tgactgtccc cagccaccac ccacatgccc 6661 gagagatgcg gaggtcagcc tcagagcaga gccccaacgt ttcccattct gcccacatga 6721 ccgagacacg cagcaaatcc tttgactatg gcagcttgtc cttgacaggc ccttctgctc 6781 cagccccagt ggctccacca gcgcgggtgg ccccgccaga gagaagaaaa tgcttcttgg 6841 tgagacaggc ctctctgagc aggcctccag aatctgagtt ggaggttgcc cccaagggaa 6901 gacaggagag cgaagaacca cagccctcat ccagtaaacc ctctgccaaa agctcattgt 6961 cccagatttc ctctgcggcc acctcacatg gtggaccccc gggaggcaag ggcccagggc 7021 aggacaggcc cccattgggg cccactgtgc cctacacaga agcactgcaa gtgttccacc 7081 accccgttgc ccagacaccc ctgcatgaga agccatacct gcccccacca gtctcccttt 7141 tctccttcca gcatctcgtg cagcatgagc caggacagtc tccagaattc ttctccaccc 7201 aggccatgtc cagcctcctg tcctcaccat actccatgcc cccacttcct ccctccttat 7261 ttcaagcccc accgcttcct ctccagccta ctgttctgca cccaggccaa ctccatctcc 7321 cccagctcat gcctcaccca gccaacatcc ccttcaggca gcccccttcc ttcctcccca 7381 tgccataccc gacctcctca gcactgtctt ctgggttttt cctgcctctg caatcccagt 7441 ttgcacttca gctccctggt gatgtggaaa gccatctgcc ccagatcaaa accagcctgg 7501 ccccactggc aacaggaagt gctggcctct cccccagcac agagtacagc agtgacatcc 7561 ggctaccccc tgtggctccc ccagccagct cctcagcacc tacatcagct cctccactgg 7621 ccctgcctgc ctgtccagac accatggtgt ccctggttgt gcctgtccgt gttcagacca 7681 atatgccgtc ctatgggagc gcaatgtaca ccaccctttc ccagatcttg gtcacccagt 7741 cccaaggcag ctcagcaact gtggcacttc ccaagtttga ggaaccccca tcaaagggga 7801 cgactgtatg tggtgcagat gtgcatgagg ttgggcccgg cccttctggg ttaagtgaag 7861 agcaaagcag agctttccca actccatacc tgagagtgcc tgtgacatta cctgaaagaa 7921 aaggcacttc cctgtcatca gagagtatct tgagcctgga ggggagttca tcaacagcag 7981 ggggaagcaa acgtgtcctt tcaccagctg gcagccttga acttaccatg gaaacccagc 8041 agcaaaaaag agtgaaggag gaggaggctt ccaaggcaga tgaaaaactt gagctggtaa 8101 aaccatgcag tgtggtcctt accagcaccg aggatgggaa gaggccagag aaatcccact 8161 taggcaacca gggccaaggc aggagggagc tagaaatgct gtccagcctg tcctcagatc 8221 catctgacac aaaggaaatt cctcccctcc ctcaccctgc attgtcccat gggacagccc 8281 caggctcaga agctttgaag gaatatcccc agccatctgg caaacctcac cgaagagggt 8341 tgaccccact gagcgtgaag aaagaagatt ccaaggaaca acctgatctc ccctccttgg 8401 cacctccgag ctctctgcct ctgtcagaaa cgtcctccag accagccaag tcacaagaag 8461 gtacggactc aaagaaggta ctgcagttcc ccagcctcca cacaaccact aatgtcagtt 8521 ggtgctattt aaactacatt aagccaaatc acatccagca tgcagatagg aggtcctctg 8581 tttacgctgg ttggtgcata agtttgtaca accccaacct tccgggggtt tccactaaag 8641 ctgctttgtc cctcctgagg tctaagcaga aagtgagcaa agagacatac accatggcca 8701 cagctccgca tcctgaggca ggaaggcttg tgccatccag ctcccgcaag ccccgcatga 8761 cagaggttca cctcccttca ctggtttccc cggaaggcca gaaagatcta gctagagtgg 8821 agaaggaaga agagaggaga ggggagccgg aggaggatgc tcctgcctcc cagagagggg 8881 agccggcgag gatcaaaatc ttcgaaggag ggtacaaatc aaacgaagag tatgtatatg 8941 tgcgaggccg cggccgaggg aaatatgttt gtgaggagtg tggaattcgc tgcaagaagc 9001 ccagcatgct gaagaaacac atccgcaccc acactgacgt ccggccctat gtgtgcaagc 9061 actgtcactt tgcttttaaa accaaaggga atctgactaa gcacatgaag tcgaaggccc 9121 acagcaaaaa gtgccaagag acaggggtgc tggaggagct ggaagccgaa gaaggaacca 9181 gtgacgacct gttccaggac tcggaaggac gagagggttc agaggctgtg gaggagcacc 9241 agttttcgga cctggaggac tcggactcag actcagacct ggacgaagac gaggatgagg 9301 atgaggagga gagccaggat gagctgtcca gaccatcctc agaggcgccc ccgcctggcc 9361 caccacatgc actgcgggca gactcctcac ccatcctggg ccctcagccc ccagatgccc 9421 ccgcctctgg cacggaggct acacgaggca gctcggtctc ggaagctgag cgcctgacag 9481 ccagcagctg ctccatgtcc agccagagca tgccgggcct cccctggctg ggaccggccc 9541 ctctgggctc tgtggagaaa gacacaggct cagccttgag ctacaagcct gtgtccccaa 9601 gaagaccgtg gtccccaagc aaagaagcag gcagccgtcc accactagcc cgcaaacact 9661 cgctaaccaa aaacgactca tctccccagc gatgctcccc ggcccgagaa ccacaggcct 9721 cagccccaag cccacctggc ctgcacgtgg acccaggaag gggcatgggc gctctccctt 9781 gtgggtctcc aagacttcag ctgtctcctc tcaccctctg ccccctggga agagaactgg 9841 cccctcgagc acatgtgctc tccaaactcg agggtaccac cgacccaggc ctccccagat 9901 actcgcccac caggagatgg tctccaggtc aggccgagtc accaccacgg tcagcgccgc 9961 cagggaagtg ggccttggct gggccgggca gcccctcagc gggggagcat ggcccaggct 10021 tggggctgga cccacgggtt ctcttcccgc ccgcgcctct acctcacaag ctcctcagca 10081 gaagcccaga gacctgcgcc tccccgtggc agaaggccga gtcccgaagt ccctcctgct 10141 cacccggccc tgctcatcct ctctcctccc gacccttctc cgccctccat gacttccacg 10201 gccacatcct ggcccggaca gaggagaaca tcttcagcca cctgcctctg cactcccagc 10261 acttgacccg tgccccatgt cccttgattc ccatcggtgg gatccagatg gtgcaggccc 10321 ggccaggagc ccaccccacc ctgctgccag ggcccaccgc agcctgggtc agtggcttct 10381 ccgggggtgg cagcgacctg acaggggccc gggaggccca ggagcgaggc cgctggagtc 10441 ccactgagag ctcgtcagcc tccgtgtcgc ctgtggctaa ggtctccaaa ttcacactct 10501 cctcagagct ggagggcggg gactacccca aggagaggga gaggaccggc ggaggcccgg 10561 gcaggcctcc tgactggaca ccccatggga ccggggcacc tgcagagccc acacccacgc 10621 acagcccctg caccccaccc gacaccttgc cccggccgcc ccagggacgc cgggcagcgc 10681 agtcctggag cccccgcttg gagtccccgc gtgcaccgac caaccccgag ccttctgcca 10741 ccccgccgct ggaccgcagc agctctgtgg gctgcctggc agaggcctct gcccgcttcc 10801 cagcccggac gaggaacctc tccggggaac ccaggaccag gcaggactcc cccaagccct 10861 caggaagtgg ggagcccagg gcacatccac atcagcctga ggacagggtt ccccccaacg 10921 ctTAGgcggc cgctggatcc tctagactgc agctcgagta cccatacgac gtcccagact 10981 acgcttgagt ttaaacacgc gtggtgtgga aagtccccag gctccccagc aggcagaagt 11041 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 11101 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 11161 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 11221 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 11281 tagtgaggag gcttttttgg aggccatgac cgagtacaag cccacggtgc gcctcgccac 11341 ccgcgacgac gtccctcggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc 11401 cacgcgccac accgtggacc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact 11461 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc 11521 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg 11581 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaagggct 11641 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc 11701 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga 11761 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga 11821 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg 11881 catgacccgc aagcccggtg cctgaacgcg ttccggaaat caacctctgg attacaaaat 11941 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc 12001 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt 12061 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg 12121 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg 12181 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc 12241 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt 12301 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct 12361 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg 12421 cggcctgctg ccggctctgc ggcctcttcc gcgtctcgcc ttcgccctca gacgagtcgg 12481 atctcccttt gggccgcctc cccgcctgtc cggatggaag ggctaattca ctcccaacga 12541 atacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg 12601 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg 12661 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc 12721 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat 12781 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat 12841 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg 12901 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gcatctatgt 12961 cgggtgcgga gaaagaggta atgaaatggc attatgggta ttatgggtct gcattaatga 13021 atcggccaac gatcccggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 13081 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 13141 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 13201 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 13261 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 13321 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 13381 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 13441 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 13501 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 13561 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 13621 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 13681 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 13741 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 13801 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 13861 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 13921 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 13981 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 14041 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 14101 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 14161 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 14221 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 14281 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgaaaaa 14341 ggatcttcac ctagatcctt ttcacgtaga aagccagtcc gcagaaacgg tgctgacccc 14401 ggatgaatgt cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc 14461 aggtagcttg cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa 14521 gcgaaccgga attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa 14581 actggatggc tttctcgccg ccaaggatct gatggcgcag gggatcaagc tctgatcaag 14641 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg 14701 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg 14761 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc 14821 tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga 14881 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc 14941 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag 15001 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat 15061 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg 15121 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca 15181 ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct 15241 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg 15301 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg 15361 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc 15421 gcatcgcctt ctatcgcctt cttgacgagt tcttctgaat tttgttaaaa tttttgttaa 15481 atcagctcat tttttaacca ataggccgaa atcggcaaca tcccttataa atcaaaagaa 15541 tagaccgcga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac 15601 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa 15661 ccatcaccca aatcaagttt tttgcggtcg aggtgccgta aagctctaaa tcggaaccct 15721 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa 15781 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc 15841 gtaaccacca cacccgcgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag 15901 gatcgaatta attcttaatt aacatcatca ataatatacc tt

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.