pLV3-CMV-Ptges-3×FLAG-mCherry-

pLV3-CMV-Ptges-3×FLAG-mCherry-Puro plasmid

PVT94836

pLV3-CMV-Ptges-3×FLAG-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-Ptges(mouse)-3×FLAG-mCherry-Puro
Alias: NM_022415.3;2410099E23Rik; D2Ertd369e; mPGES; mPGES-1;Pges
Gene lenght: 459bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Ptges-3×FLAG-mCherry-Puro plasmid Description

pLV3-CMV-Ptges-3×FLAG-mCherry-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Ptges-3×FLAG-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatgccttc cccgggcctg gtgatggaga gcggccaggt 2341 gctcccggcc tttctgctct gcagcacact gctggtcatc aagatgtacg cggtggctgt 2401 catcacaggc cagatgaggc tgcggaagaa ggcttttgcc aaccccgagg atgcgctgaa 2461 acgtggaggc ctccagtatt acaggagtga cccagatgtg gagcgctgcc tcagagccca 2521 ccgcaacgac atggagacaa tctatccttt cctcttcctc ggcttcgtgt actcattcct 2581 gggacccaac cctctgatcg cctggataca tttcctcgtt gtcctcacag gccgtgtggt 2641 acacaccgtg gcctacctgg gcaagctgaa cccacgcctt cgctccgggg cctatgtcct 2701 ggcccagttc tcctgtttct ccatggccct gcagatcctc tgggaggtgg cccaccatct 2761 gggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA 2821 TGACGATGAC AAGtagcggc cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc 2881 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg 2941 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 3001 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 3061 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc 3121 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc 3181 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc 3241 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac 3301 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg 3361 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggtga gcaagggcga 3421 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc 3481 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac 3541 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct 3601 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga 3661 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga 3721 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa 3781 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat 3841 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat 3901 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta 3961 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat 4021 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca 4081 ctccaccggc ggcatggacg agctgtacaa gggatctcgc gagggcagag gaagtcttct 4141 aacatgcggt gacgtggagg agaatcccgg ccctatgacc gagtacaagc ccacggtgcg 4201 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga 4261 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct 4321 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga 4381 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc 4441 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat 4501 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg 4561 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga 4621 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc 4681 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg 4741 cacctggtgc atgacccgca agcccggtgc ctgaaatcaa cctctggatt acaaaatttg 4801 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc 4861 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta 4921 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt 4981 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca 5041 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc 5101 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt 5161 gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg 5221 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg 5281 cctgctgccg gctctgcggc ctcttccgcg tctccgcctt cgccctcaga cgagtcggat 5341 ctccctttgg ccgcctcccc gcctggtacc tttaagacca atgacttaca aggcagctgt 5401 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg 5461 aaaataagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg 5521 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt 5581 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc 5641 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta 5701 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta 5761 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 5821 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct 5881 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt 5941 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga 6001 ggcttttttg gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag 6061 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 6121 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 6181 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa 6241 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 6301 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 6361 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 6421 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 6481 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 6541 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 6601 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 6661 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 6721 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 6781 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 6841 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 6901 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 6961 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 7021 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 7081 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 7141 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 7201 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 7261 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 7321 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 7381 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 7441 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 7501 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt 7561 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg 7621 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc 7681 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc 7741 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg 7801 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga 7861 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 7921 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 7981 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 8041 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 8101 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 8161 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc 8221 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg 8281 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct 8341 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc 8401 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat 8461 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg 8521 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 8581 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 8641 cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.