pLV2-CMV-Cd19-mCherry-Puro pla

pLV2-CMV-Cd19-mCherry-Puro plasmid

PVT96886

pLV2-CMV-Cd19-mCherry-Puro plasmid Information

Plasmid name: pLV2-CMV-Cd19(mouse)-mCherry-Puro
Alias: NM_009844.3
Gene lenght: 1641bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-Cd19-mCherry-Puro plasmid Description

pLV2-CMV-Cd19-mCherry-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV2-CMV-Cd19-mCherry-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatgccatc tcctctccct gtctccttcc tcctctttct 2881 taccttagta ggaggcaggc cccagaagtc cttactggtg gaggtagaag agggaggcaa 2941 tgttgtgctg ccatgcctcc cggactcctc acctgtctct tctgagaagc tggcttggta 3001 tcgaggtaac cagtcaacac ccttcctgga gctgagcccc gggtcccctg gcctgggatt 3061 gcacgtgggg tccctgggca tcttgctagt gattgtcaat gtctcagacc atatgggggg 3121 cttctacctg tgccagaaga ggcccccttt caaggacatc tggcagcctg cctggacagt 3181 gaacgtggag gatagtgggg agatgttccg gtggaatgct tcagacgtca gggacctgga 3241 ctgtgaccta aggaacaggt cctctgggag ccacaggtcc acttctggtt cccagctgta 3301 tgtgtgggct aaagaccatc ctaaggtctg gggaacaaag cctgtatgtg cccctcgggg 3361 gagcagtttg aatcagagtc taatcaacca agatctcact gtggcacccg gctccacact 3421 ttggctgtcc tgtggggtac cccctgtccc agtggccaaa ggctccatct cctggaccca 3481 tgtgcatcct aggagaccta atgtttcact actgagccta agccttgggg gagagcaccc 3541 ggtcagagag atgtgggttt gggggtctct tctgcttctg ccccaagcca cagctttaga 3601 tgaaggcacc tattattgtc tccgaggaaa cctgaccatc gagaggcacg tgaaggtcat 3661 tgcaaggtca gcagtgtggc tctggctgtt gagaactggt ggatggatag tcccagtggt 3721 gactttagta tatgtcatct tctgtatggt ttctctggtg gcttttctct attgtcaaag 3781 agcctttatc ctgagaagga aaaggaagcg aatgactgac cccgccagga gattcttcaa 3841 agtgacgcct ccctcgggaa acgggaccca gaaccagtac gggaatgtgc tctcccttcc 3901 tacatctacc tctggccagg cccatgctca gcgttgggct gctggcctag ggagtgtccc 3961 tgggtcctat ggaaatccac gcattcaagt ccaggatact ggagctcaga gccatgaaac 4021 aggactggaa gaagaagggg aggcctatga agagccagac agcgaggagg gctctgaatt 4081 ctatgagaac gactccaacc ttgggcagga ccaggtttcc caggatggga gtggctatga 4141 gaaccccgag gatgagccca tgggtccaga ggaagaagac tccttctcca atgctgagtc 4201 ttatgaaaat gcagatgagg agctggccca accagttggc aggatgatgg acttcctgag 4261 cccccatggg tctgcgtggg accccagccg ggaagcatcc tcgcttgggt cccagtccta 4321 tgaagatatg agagggatcc tctatgcagc tcctcagctc cactcaattc agtccggtcc 4381 cagtcatgaa gaagatgcag actcttatga aaacatggat aagtctgacg acctagaacc 4441 agcatgggaa ggagagggcc acatggggac ttggggaacc acgggtggag gcggatcagg 4501 aggcggtgga tctatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt 4561 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg 4621 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg 4681 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc 4741 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt 4801 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc 4861 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc 4921 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta 4981 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg 5041 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg 5101 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt 5161 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa 5221 gtagggatcc cgcgactcta gataattcta ccgggtaggg gaggcgcttt tcccaaggca 5281 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg 5341 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc 5401 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc 5461 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag 5521 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc 5581 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg 5641 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca 5701 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag 5761 cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc 5821 cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc 5881 gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc 5941 gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg 6001 accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc 6061 gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac 6121 cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc 6181 aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg 6241 cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc 6301 accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag 6361 cccggtgcct gaccgcgtct ggaacaatca acctctggat tacaaaattt gtgaaagatt 6421 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc 6481 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg 6541 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac 6601 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc 6661 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc 6721 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa 6781 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc 6841 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc 6901 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg 6961 ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca tggagcaatc 7021 acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag 7081 gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca 7141 gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc 7201 caacgaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat 7261 tagcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac 7321 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc 7381 ttgttacacc ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg 7441 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc 7501 aagaactgct gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg 7561 cgtggcctgg gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct 7621 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta 7681 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg 7741 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg 7801 gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa 7861 gaaatgaata tcagagagtg agaggccttg acattgctag cgttttaccg tcgacctcta 7921 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 7981 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 8041 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 8101 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 8161 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 8221 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 8281 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 8341 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 8401 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 8461 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 8521 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 8581 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 8641 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 8701 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 8761 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 8821 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 8881 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 8941 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 9001 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 9061 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 9121 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 9181 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 9241 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 9301 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 9361 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 9421 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 9481 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 9541 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 9601 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 9661 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 9721 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 9781 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 9841 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 9901 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 9961 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 10021 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 10081 gaaaagtgcc acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat 10141 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 10201 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata 10261 actcaagcta accaaaatca tcccaaactt cccaccccat accctattac cactgccaat 10321 tacctgtggt ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga 10381 aattgtattt gttaaatatg tactacaaac ttagtagttt ttaaagaaat tgtatttgtt 10441 aaatatgtac tacaaactta gtagt

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.