pLV3-CMV-MBNL2-3×FLAG-EF1a-Cop

pLV3-CMV-MBNL2-3×FLAG-EF1a-CopGFP-Neo plasmid

PVT94718

pLV3-CMV-MBNL2-3×FLAG-EF1a-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-MBNL2(human)-3×FLAG-EF1a-CopGFP-Neo
Alias: NM_144778.4;MBLL; MBLL39; PRO2032
Gene lenght: 1101bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-MBNL2-3×FLAG-EF1a-CopGFP-Neo plasmid Description

pLV3-CMV-MBNL2-3×FLAG-EF1a-CopGFP-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-MBNL2-3×FLAG-EF1a-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggcttt gaacgttgcc ccagtcagag atacaaaatg 2341 gctgacatta gaagtctgca gacagtttca aagaggaaca tgctcacgct ctgatgaaga 2401 atgcaaattt gctcatcccc ccaaaagttg tcaggttgaa aatggaagag taattgcctg 2461 ctttgattcc ctaaagggcc gttgttcgag agagaactgc aagtatcttc accctccgac 2521 acacttaaaa actcaactag aaattaatgg aaggaacaat ttgattcagc aaaaaactgc 2581 agcagcaatg cttgcccagc agatgcaatt tatgtttcca ggaacaccac ttcatccagt 2641 gcccactttc cctgtaggtc ccgcgatagg gacaaatacg gctattagct ttgctcctta 2701 cctagcacct gtaacccctg gagttgggtt ggtcccaacg gaaattctgc ccaccacgcc 2761 tgttattgtt cccggaagtc caccggtcac tgtcccgggc tcaactgcaa ctcagaaact 2821 tctcaggact gacaaactgg aggtatgcag ggagttccag cgaggaaact gtgcccgggg 2881 agagaccgac tgccgctttg cacaccccgc agacagcacc atgatcgaca caagtgacaa 2941 caccgtaacc gtttgtatgg attacataaa ggggcgttgc atgagggaga aatgcaaata 3001 ttttcaccct cctgcacact tgcaggccaa aatcaaagct gcgcagcacc aagccaacca 3061 agctgcggtg gccgcccagg cagccgcggc cgcggccaca gtcatggcct ttccccctgg 3121 tgctcttcat cctttaccaa agagacaagc acttgaaaaa agcaatggta ccagcgcggt 3181 ctttaacccc agcgtcttgc actaccagca ggctctcacc agcgcacagt tgcagcaaca 3241 cgccgcgttc attccaacag ggtcagtttt gtgcatgaca cccgctacca gtattgtacc 3301 catgatgcac agcgctacgt ccgccactgt ctctgcagca acaactcctg caacaagtgt 3361 ccccttcgca gcaacagcca cagccaatca gataattctg aaaggatccG ACTACAAAGA 3421 CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGTAgcg 3481 gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac 3541 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg 3601 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg 3661 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc 3721 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac 3781 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc 3841 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc 3901 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct 3961 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg 4021 accggcgcct acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga 4081 gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg 4141 agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct 4201 gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac 4261 ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa 4321 cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta 4381 cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag 4441 cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat 4501 gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta 4561 ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct 4621 gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga 4681 gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg 4741 cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg 4801 cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctGCCAC 4861 Catggctgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 4921 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 4981 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 5041 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 5101 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 5161 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 5221 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 5281 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 5341 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 5401 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 5461 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 5521 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 5581 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 5641 tgacgagttc ttctgaGTCG ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC 5701 TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT 5761 GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT 5821 GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT 5881 GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG 5941 GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG 6001 CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC 6061 ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT 6121 CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC 6181 TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC 6241 CGCCTCCCCG Cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac 6301 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaaG ataagatctg 6361 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 6421 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 6481 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 6541 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 6601 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa 6661 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 6721 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa 6781 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 6841 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 6901 gcctagactt ttgcagagac ggcccaaatt cgtaatcatg gtcatagctg tttcctgtgt 6961 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 7021 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 7081 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 7141 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 7201 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 7261 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 7321 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 7381 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 7441 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 7501 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 7561 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 7621 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 7681 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 7741 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 7801 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 7861 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 7921 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 7981 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 8041 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 8101 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 8161 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 8221 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 8281 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 8341 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 8401 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 8461 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 8521 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 8581 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 8641 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 8701 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 8761 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 8821 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 8881 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 8941 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 9001 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 9061 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 9121 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg 9181 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 9241 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct 9301 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa 9361 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc 9421 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 9481 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 9541 gtaaaacgac ggccagtgcc aagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.