pLV3-CMV-Ggt5-3×FLAG-CopGFP-Pu

pLV3-CMV-Ggt5-3×FLAG-CopGFP-Puro plasmid

PVT96187

pLV3-CMV-Ggt5-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Ggt5(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_011820.6;GGL; GGT-REL; Ggtla1
Gene lenght: 1722bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Ggt5-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Ggt5-3×FLAG-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Ggt5-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg cttggggtca cagggccacg 2341 gtctgcctgg tcctgctggg tgtaggtcta ggtctggtta tcgttgtgtt ggctgcggtc 2401 ctttctcctc gtcaagcctc ttgtggtccc ggtgccttca cgcgtgctgc ggtagcggct 2461 gactccaaga tctgctcgga tattggacga gccatcctcc agcagcgggg ctcacctgtg 2521 gatgccgcca tcgctgccct ggtctgtact ggtgttgtta accctcagag catgggtctg 2581 ggcggagggg ttgtcttcac catctacaat gcatcaacag gcaaggtaga gatcatcaat 2641 gcacgggaga cagtgccagc cagctatgac caaggcctgt tgaaccagtg taaaaacgtc 2701 ctgccattgg gcacaggggc ccagtggatc ggggttcctg gggagctccg cggctatgct 2761 gaggcccacc gccgccacgg ccgcctgcct tgggctcagc ttttccagcc caccattgca 2821 ctgctccgag agggtttccg tgtgcccttt atcctcagcc aattcctcaa caacagcatc 2881 ctgcggcccc acttgagtgc atcaaccctg agacagctct tcttcaatgg gacagaaacc 2941 ttgagatctc aggacccatt cccatggcct gcgctggcca acaccctgga gactgtagcc 3001 aaggagggtg cggaggtctt atacacaggg agactgggcc gcatgctggt ggaagacatt 3061 gccaagcaag gaagcctgct gacagttcag gacttggctg ccttccagcc tgaagtggtg 3121 gaacccctgg agatgcccct aggaaactat accctgtact cgccgccgcc gcctgcaggg 3181 ggcgctattc ttagcttcat tctcaatgtg ctgaaagggt tcaacttctc tgcagagacg 3241 gtggcgcggc ctggagggga agtgaacatg taccatcacc ttgtagagac tctcaagttc 3301 gccgtagggc agaggtggcg gctatgggac ccttccagtc acccggggat acagaacatc 3361 tcccgggacc tcctccggga agatctagcc cagcgcatcc gccagcagat tgacggccgt 3421 ggtgaccacc accagctcag ccattacaac ctgactgggg tcaggggcaa ccggatgggc 3481 acctctcatg tgtctgtgtt gggagaagat ggcagtgctg tggcagccac cagcaccatc 3541 aacacaccct ttggggcgat ggtgtactcc ccacggacag gcatcctcct gaacaacgag 3601 cttctagact tatgctggag acacatgcca acatccccga tcacccctcc acctgttcca 3661 ggtgagcggc caccatcttc catggtacct tccatcttgg tcaacaaagg ccagggatcg 3721 aagctggtga tcggtggggc aggtggagag ctgatcatct ctgccgtggc ccagactatc 3781 atgaacaagc tgtggcttgg ctttgacctg acagaagcta tcgcatctcc tattctacac 3841 gtcaacagca agggccatgt ggagtatgag cccaaattca accaggaggt acagaagggg 3901 ctacaggacc gaggccagat tcagagccag agccagcggc cagttttcct gaatgcggtc 3961 caggctgtgt ttcaggaagg gccctgcgtc tatgcagcgt ctgacctgag gaaggctgga 4021 aaggcttcag gatacggatc cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 4081 ATCGACTACA AGGATGACGA TGACAAGtag cggccgcGaa ggatctgcga tcgctccggt 4141 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 4201 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 4261 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 4321 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 4381 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 4441 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 4501 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 4561 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 4621 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 4681 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 4741 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 4801 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 4861 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 4921 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 4981 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 5041 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 5101 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 5161 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 5221 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 5281 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 5341 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 5401 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 5461 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 5521 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 5581 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 5641 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 5701 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 5761 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 5821 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 5881 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 5941 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 6001 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 6061 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 6121 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 6181 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 6241 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 6301 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 6361 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 6421 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 6481 aaATCATCGT CCTTTCCTtg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 6541 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 6601 ccggctctgc ggcctcttcc gcgtctTcgc cttcgccctc agacgagtcg gatctccctt 6661 tggGccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct 6721 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata 6781 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 6841 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 6901 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 6961 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 7021 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 7081 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 7141 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctaTCCCG 7201 CCCCTAACTC CGCCCAtccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 7261 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 7321 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat 7381 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 7441 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 7501 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 7561 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 7621 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 7681 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 7741 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 7801 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 7861 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 7921 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 7981 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 8041 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 8101 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 8161 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 8221 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 8281 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 8341 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 8401 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 8461 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 8521 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 8581 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 8641 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 8701 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 8761 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 8821 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 8881 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 8941 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 9001 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 9061 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 9121 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 9181 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 9241 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 9301 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 9361 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 9421 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 9481 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 9541 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 9601 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 9661 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 9721 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 9781 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 9841 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 9901 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 9961 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.