pLV3-CMV-Slc25a51-3×FLAG-CopGF

pLV3-CMV-Slc25a51-3×FLAG-CopGFP-Puro plasmid

PVT94705

pLV3-CMV-Slc25a51-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Slc25a51(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_001009949.3;9130208E07Rik; D130005A03Rik; Gm138;Mcart1
Gene lenght: 894bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Slc25a51-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Slc25a51-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Slc25a51-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatga tggactccga agcacatgag 2341 aagaggcccc caatgttgac ctcttcgaac caggacttgt cacctcacat tgcgggcgtg 2401 ggagacatga aacattactt gtgtggctac tgcgcggcct tcaacaacgt ggcgatcact 2461 tacccggttc agaagatcct cttccggcag cagctttacg gcatcaagac ccgggatgca 2521 gtcctgcagc tgaggaagga tgggttccgc aacttgtacc gcggaatcct gcccccgctg 2581 atgcagaaga cgaccacgct ggcacttatg tttggtctgt atgaggacct gtcgcgcctg 2641 ctccacaagc acgtgagcag cgctcccgag tttgccaccc gtagcgtggc ggcgctgctg 2701 gccgggacga cggaagccat tctcactcca ttcgaaaggg ttcagacttt gcttcaggac 2761 cacaaacatc acgataagtt cacaaacact taccaggcct tcagggcgct cagatgccat 2821 gggattgcag agtattaccg aggcatggtg cccatccttt tccggaatgg gttcggcaat 2881 gtcctttttt ttggccttcg agggcccatt aaggagagcc tgcctactgc cactacatac 2941 agcgcccacc tggtcaacga cttcatctgt ggaggtgtac tcggggccgt gctgggcttc 3001 ttaagcttcc ccattaacgt tgtcaaagct cgaatacagt ctcagattgg cgggccattc 3061 ctgtctctcc ccatggtctt caagactatc tggatagagc gggacaggaa gctgatcaat 3121 ctcttcagag gtgcacatct gaattaccac cgctctctca tctcctgggg aataatcaat 3181 gccacttacg agtttttgtt aaagattgta ggatccGACT ACAAAGACCA TGACGGTGAT 3241 TATAAAGATC ATGACATCGA CTACAAGGAT GACGATGACA AGtagcggcc gcGaaggatc 3301 tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag 3361 ttggggggag gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg 3421 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata 3481 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg 3541 aagcttcgag gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat 3601 ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc 3661 gccgtctagg taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg 3721 agcctaccta gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac 3781 gtctttgttt cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg 3841 ctagacgcca ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc 3901 atcaccggca ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc 3961 aagcagggcc gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc 4021 tacctgctga gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc 4081 tacgagaacc ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag 4141 aagtacgagg acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc 4201 gtgatcggcg acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc 4261 gacaagatca tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg 4321 ctggtgggca gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg 4381 gtggacagcc acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc 4441 cccatgttcg ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg 4501 gagtaccagc acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc 4561 aattctgccg tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga 4621 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc 4681 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg 4741 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc 4801 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 4861 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 4921 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 4981 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 5041 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 5101 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 5161 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 5221 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac 5281 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga 5341 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc 5401 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa 5461 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc 5521 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc 5581 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc 5641 gtggtgttgt cggggaaATC ATCGTCCTTT CCTtggctgc tcgcctgtgt tgccacctgg 5701 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct 5761 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tTcgccttcg ccctcagacg 5821 agtcggatct ccctttggGc cgcctccccg cctggtacct ttaagaccaa tgacttacaa 5881 ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca 5941 ctcccaacga aGataagatc tgctttttgc ttgtactggg tctctctggt tagaccagat 6001 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt 6061 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc 6121 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat 6181 tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat 6241 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 6301 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 6361 gctctagcta TCCCGCCCCT AACTCCGCCC Atcccgcccc taactccgcc cagttccgcc 6421 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 6481 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga cttttgcaga 6541 gaccaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat 6601 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag 6661 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg 6721 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc 6781 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 6841 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 6901 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 6961 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 7021 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 7081 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 7141 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 7201 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 7261 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 7321 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 7381 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 7441 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 7501 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 7561 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 7621 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 7681 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 7741 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 7801 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 7861 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 7921 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 7981 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 8041 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 8101 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 8161 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 8221 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 8281 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 8341 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 8401 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 8461 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 8521 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 8581 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 8641 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 8701 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 8761 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 8821 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 8881 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 8941 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 9001 aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 9061 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 9121 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa 9181 gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.