pCMV-NCOA1-3×FLAG-Neo plasmid

pCMV-NCOA1-3×FLAG-Neo plasmid

PVT94858

pCMV-NCOA1-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-NCOA1(human)-3×FLAG-Neo
Alias: NM_003743.5;bHLHe42; bHLHe74; F-SRC-1; KAT13A; RIP160;SRC1
Gene lenght: 4323bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-NCOA1-3×FLAG-Neo plasmid Description

pCMV-NCOA1-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-NCOA1-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgag tggcctcggg gacagttcat 781 ccgaccctgc taacccagac tcacataaga ggaaaggatc gccatgtgac acactggcat 841 caagcacgga aaagaggcgc agggagcaag aaaataaata tttagaagaa ctagctgagt 901 tactgtctgc caacattagt gacattgaca gcttgagtgt aaaaccagac aaatgcaaga 961 ttttgaagaa aacagtcgat cagatacagc taatgaagag aatggaacaa gagaaatcaa 1021 caactgatga cgatgtacag aaatcagaca tctcatcaag tagtcaagga gtgatagaaa 1081 aggaatcctt gggacctctt cttttggagg ctttggatgg atttttcttt gttgtgaact 1141 gtgaagggag aattgtattt gtgtcagaga atgtaaccag ctacttaggt tacaatcagg 1201 aggaattaat gaatacgagc gtctacagca tactgcacgt gggggatcat gcagaatttg 1261 tgaagaatct gctaccaaaa tcactagtaa atggagttcc ttggcctcaa gaggcaacac 1321 gacgaaatag ccataccttt aactgcagga tgctaattca ccctccagat gagccaggga 1381 ccgagaacca agaagcttgc cagcgttatg aagtaatgca gtgtttcact gtgtcacagc 1441 caaaatcaat tcaagaggat ggagaagatt tccagtcatg tctgatttgt attgcacggc 1501 gattacctcg gcctccagct attacgggtg tagaatcctt tatgaccaag caagatacta 1561 caggtaaaat catctctatt gatactagtt ccctgagagc tgctggcaga actggttggg 1621 aagatttagt gaggaagtgc atttatgctt ttttccaacc tcagggcaga gaaccatctt 1681 atgccagaca gctgttccaa gaagtgatga ctcgtggcac tgcctccagc ccctcctata 1741 gattcatatt gaatgatggg acaatgctta gcgcccacac caagtgtaaa ctttgctacc 1801 ctcaaagtcc agacatgcaa cctttcatca tgggaattca tatcatcgac agggagcaca 1861 gtgggctttc tcctcaagat gacactaatt ctggaatgtc aattccccga gtaaatccct 1921 cggtcaatcc tagtatctct ccagctcatg gtgtggctcg ttcatccaca ttgccaccat 1981 ccaacagcaa catggtatcc accagaataa accgccagca gagctcagac cttcatagca 2041 gcagtcatag taattctagc aacagccaag gaagtttcgg atgctcaccc ggaagtcaga 2101 ttgtagccaa tgttgcctta aaccaaggac aggccagttc acagagcagt aatccctctt 2161 taaacctcaa taattctcct atggaaggta caggaatatc cctagcacag ttcatgtctc 2221 caaggagaca ggttacttct ggattggcaa caaggcccag gatgccaaac aattcctttc 2281 ctcctaatat ttcgacatta agctctcccg ttggcatgac aagtagtgcc tgtaataata 2341 ataaccgatc ttattcaaac atcccagtaa catctttaca gggtatgaat gaaggaccca 2401 ataactccgt tggcttctct gccagttctc cagtcctcag gcagatgagc tcacagaatt 2461 cacctagcag attaaatata caaccagcaa aagctgagtc caaagataac aaagagattg 2521 cctcaatttt aaatgaaatg attcaatctg acaacagctc tagtgatggc aaacctctgg 2581 attcagggct tctgcataac aatgacagac tttcagatgg agacagtaaa tactctcaaa 2641 ccagtcacaa actagtgcag cttttgacaa caactgccga acagcagtta cggcatgctg 2701 atatagacac aagctgcaaa gatgtcctgt cttgcacagg cacttccaac tctgcctctg 2761 ctaactcttc aggaggttct tgtccctctt ctcatagctc attgacagaa cggcataaaa 2821 ttctacaccg gctcttacag gagggtagcc cctcagatat caccactttg tctgtcgagc 2881 ctgataaaaa ggacagtgca tctacttctg tgtcagtgac tggacaggta caaggaaact 2941 ccagtataaa actagaactg gatgcttcaa agaaaaaaga atcaaaagac catcagctcc 3001 tacgctatct tttagataaa gatgagaaag atttaagatc aactccaaac ctgagcctgg 3061 atgatgtaaa ggtgaaagtg gaaaagaaag aacagatgga tccatgtaat acaaacccaa 3121 ccccaatgac caaacccact cctgaggaaa taaaactgga ggcccagagc cagtttacag 3181 ctgaccttga ccagtttgat cagttactgc ccacgctgga gaaggcagca cagttgccag 3241 gcttatgtga gacagacagg atggatggtg cggtcaccag tgtaaccatc aaatcggaga 3301 tcctgccagc ttcacttcag tccgccactg ccagacccac ttccaggcta aatagattac 3361 ctgagctgga attggaagca attgataacc aatttggaca accaggaaca ggcgatcaga 3421 ttccatggac aaataataca gtgacagcta taaatcagag taaatcagaa gaccagtgta 3481 ttagctcaca attagatgag cttctctgtc cacccacaac agtagaaggg agaaatgatg 3541 agaaggctct tcttgaacag ctggtatcct tccttagtgg caaagatgaa actgagctag 3601 ctgaactaga cagagctctg ggaattgaca aacttgttca ggggggtgga ttagatgtat 3661 tatcagagag atttccacca caacaagcaa cgccaccttt gatcatggaa gaaagaccca 3721 acctttattc ccagccttac tcttctcctt ctcctactgc caatctccct agccctttcc 3781 aaggcatggt caggcaaaaa ccttcactgg ggacgatgcc tgttcaagta acacctcccc 3841 gaggtgcttt ttcacctggc atgggcatgc agcccaggca aactctaaac agacctccgg 3901 ctgcacctaa ccagcttcga cttcaactac agcagcgatt acagggacaa cagcagttga 3961 tacaccaaaa tcggcaagct atcttaaacc agtttgcagc aactgctcct gttggcatca 4021 atatgagatc aggcatgcaa cagcaaatta cacctcagcc acccctgaat gctcaaatgt 4081 tggcacaacg tcagcgggaa ctgtacagtc aacagcaccg acagaggcag ctaatacagc 4141 agcaaagagc catgcttatg aggcagcaaa gctttgggaa caacctccct ccctcatctg 4201 gactaccagt tcaaatgggg aacccccgtc ttcctcaggg tgctccacag caattcccct 4261 atccaccaaa ctatggtaca aatccaggaa ccccacctgc ttctaccagc ccgttttcac 4321 aactagcagc aaatcctgaa gcatccttgg ccaaccgcaa cagcatggtg agcagaggca 4381 tgacaggaaa cataggagga cagtttggca ctggaatcaa tcctcagatg cagcagaatg 4441 tcttccagta tccaggagca ggaatggttc cccaaggtga ggccaacttt gctccatctc 4501 taagccctgg gagctccatg gtgccgatgc caatccctcc tcctcagagt tctcttctcc 4561 agcaaactcc acctgcctcc gggtatcagt caccagacat gaaggcctgg cagcaaggag 4621 cgataggaaa caacaatgtg ttcagtcaag ctgtccagaa ccagcccacg cctgcacagc 4681 caggagtata caacaacatg agcatcaccg tttccatggc aggtggaaat acgaatgttc 4741 agaacatgaa cccaatgatg gcccagatgc agatgagctc tttgcagatg ccaggaatga 4801 acactgtgtg ccctgagcag ataaatgatc ccgcactgag acacacaggc ctctactgca 4861 accagctctc atccactgac cttctcaaaa cagaagcaga tggaacccag caggtgcaac 4921 aggttcaggt gtttgctgac gtccagtgta cagtgaatct ggtaggcggg gacccttacc 4981 tgaaccagcc tggtccactg ggaactcaaa agcccacgtc aggaccacag accccccagg 5041 cccagcagaa gagcctcctt cagcagctac tgactgaaGG ATCCGACTAC AAAGACCATG 5101 ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG TAGTGAGGGC 5161 CCGATATCTC GACAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT 5221 TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC 5281 TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT 5341 TTATGAGGAG TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA 5401 CGCAACCCCC ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC 5461 TTTCCCCCTC CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC 5521 AGGGGCTCGG CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAGC TGACGTCCTT 5581 TCCATGGCTG CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT 5641 CCCTTCGGCC CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC 5701 TCTTCCGCGT CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC 5761 GCCTGGAAAC GGGGGAGGCT AACTGAAACA CGGAAGGAGA CAATACCGGA AGGAACCCGC 5821 GCTATGACGG CAATAAAAAG ACAGAATAAA ACGCACGGGT GTTGGGTCGT TTGTTCATAA 5881 ACGCGGGGTT CGGTCCCAGG GCTGGCACTC TGTCGATACC CCACCGAGAC CCCATTGGGG 5941 CCAATACGCC CGCGTTTCTT CCTTTTCCCC ACCCCACCCC CCAAGTTCGG GTGAAGGCCC 6001 AGGGCTCGCA GCCAACGTCG GGGCGGCAGG CCCTGCCATA GCAGATCTGC GCAGCTGGGG 6061 CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT 6121 TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT 6181 CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGCATCCC 6241 TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA 6301 TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC 6361 CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT 6421 CTATTCTTTT GATTTATAAG GGATTTTGGG GATTTCGGCC TATTGGTTAA AAAATGAGCT 6481 GATTTAACAA AAATTTAACG CGAATTAATT CTGTGGAATG TGTGTCAGTT AGGGTGTGGA 6541 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 6601 ACCAGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 6661 AATTAGTCAG CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC 6721 AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG 6781 GCCGCCTCTG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC 6841 TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA 6901 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG 6961 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC 7021 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT 7081 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT 7141 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC 7201 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC 7261 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC 7321 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG 7381 GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG 7441 GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT 7501 ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG 7561 GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA 7621 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC 7681 TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGCG AAATGACCGA 7741 CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG 7801 GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT 7861 CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA 7921 AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG 7981 TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG 8041 CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC 8101 ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA 8161 ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA 8221 GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC 8281 CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC 8341 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 8401 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 8461 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 8521 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 8581 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 8641 GGCGCTTTCT CAATGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 8701 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 8761 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 8821 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 8881 CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 8941 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 9001 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 9061 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 9121 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 9181 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC 9241 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA 9301 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA 9361 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG 9421 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC 9481 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT 9541 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG 9601 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT 9661 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA 9721 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA 9781 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA 9841 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG 9901 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC 9961 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG 10021 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT 10081 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT 10141 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT 10201 GCCACCTGAC GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTCGAC TCTCAGTACA 10261 ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC 10321 GCTGAGTAGT GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC 10381 ATGAAGAATC TGCTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.