pLV3-CMV-Slc25a51-3×FLAG-ZsGre

pLV3-CMV-Slc25a51-3×FLAG-ZsGreen1-Puro plasmid

PVT96359

pLV3-CMV-Slc25a51-3×FLAG-ZsGreen1-Puro plasmid Information

Plasmid name: pLV3-CMV-Slc25a51(mouse)-3×FLAG-ZsGreen1-Puro
Alias: NM_001009949.3;9130208E07Rik; D130005A03Rik; Gm138;Mcart1
Gene lenght: 894bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Slc25a51-3×FLAG-ZsGreen1-Puro plasmid Description

pLV3-CMV-Slc25a51-3×FLAG-ZsGreen1-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-Slc25a51-3×FLAG-ZsGreen1-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca 1441 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg 1501 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa 1561 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 1621 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 1681 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag 1741 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc 1801 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata 1861 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aAATTTTCGG 1921 GTTTATTACA GGGACAGCAG AGATCCAGTT TATCGATAAG CTTGGGAGTT CCGCGTTACA 1981 TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA 2041 ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG 2101 GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG 2161 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC 2221 TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG 2281 ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA 2341 AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT 2401 CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG 2461 GAGGTCTATA TAAGCAGAGC TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA 2521 CGCTGTTTTG ACCTCCATAG AAGACACCGA CTCTACTAGA GGATCTATTT CCGGTGAATT 2581 CGCCACCatg atggactccg aagcacatga gaagaggccc ccaatgttga cctcttcgaa 2641 ccaggacttg tcacctcaca ttgcgggcgt gggagacatg aaacattact tgtgtggcta 2701 ctgcgcggcc ttcaacaacg tggcgatcac ttacccggtt cagaagatcc tcttccggca 2761 gcagctttac ggcatcaaga cccgggatgc agtcctgcag ctgaggaagg atgggttccg 2821 caacttgtac cgcggaatcc tgcccccgct gatgcagaag acgaccacgc tggcacttat 2881 gtttggtctg tatgaggacc tgtcgcgcct gctccacaag cacgtgagca gcgctcccga 2941 gtttgccacc cgtagcgtgg cggcgctgct ggccgggacg acggaagcca ttctcactcc 3001 attcgaaagg gttcagactt tgcttcagga ccacaaacat cacgataagt tcacaaacac 3061 ttaccaggcc ttcagggcgc tcagatgcca tgggattgca gagtattacc gaggcatggt 3121 gcccatcctt ttccggaatg ggttcggcaa tgtccttttt tttggccttc gagggcccat 3181 taaggagagc ctgcctactg ccactacata cagcgcccac ctggtcaacg acttcatctg 3241 tggaggtgta ctcggggccg tgctgggctt cttaagcttc cccattaacg ttgtcaaagc 3301 tcgaatacag tctcagattg gcgggccatt cctgtctctc cccatggtct tcaagactat 3361 ctggatagag cgggacagga agctgatcaa tctcttcaga ggtgcacatc tgaattacca 3421 ccgctctctc atctcctggg gaataatcaa tgccacttac gagtttttgt taaagattgt 3481 aGGATCCTCG GTACCAAGCT TAAGTGACTA CAAGGATGAC GATGACAAGG ATTACAAAGA 3541 CGACGATGAT AAGGACTATA AGGATGATGA CGACAAATAA AGATCCATCG ATACTAGTAA 3601 GGATCTGCGA TCGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG 3661 AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CGGGTGCCTA GAGAAGGTGG CGCGGGGTAA 3721 ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT 3781 ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC 3841 AGCTGAAGCT TCGAGGGGCT CGCATCTCTC CTTCACGCGC CCGCCGCCCT ACCTGAGGCC 3901 GCCATCCACG CCGGTTGAGT CGCGTTCTGC CGCCTCCCGC CTGTGGTGCC TCCTGAACTG 3961 CGTCCGCCGT CTAGGTAAGT TTAAAGCTCA GGTCGAGACC GGGCCTTTGT CCGGCGCTCC 4021 CTTGGAGCCT ACCTAGACTC AGCCGGCTCT CCACGCTTTG CCTGACCCTG CTTGCTCAAC 4081 TCTACGTCTT TGTTTCGTTT TCTGTTCTGC GCCGTTACAG ATCCAAGCTG TGACCGGCGC 4141 CTACTCTAGC ATGGCCCAGT CCAAGCACGG CCTGACCAAG GAGATGACCA TGAAGTACCG 4201 CATGGAGGGC TGCGTGGACG GCCACAAGTT CGTGATCACC GGCGAGGGCA TCGGCTACCC 4261 CTTCAAGGGC AAGCAGGCCA TCAACCTGTG CGTGGTGGAG GGCGGCCCCT TGCCCTTCGC 4321 CGAGGACATC TTGTCCGCCG CCTTCATGTA CGGCAACCGC GTGTTCACCG AGTACCCCCA 4381 GGACATCGTC GACTACTTCA AGAACTCCTG CCCCGCCGGC TACACCTGGG ACCGCTCCTT 4441 CCTGTTCGAG GACGGCGCCG TGTGCATCTG CAACGCCGAC ATCACCGTGA GCGTGGAGGA 4501 GAACTGCATG TACCACGAGT CCAAGTTCTA CGGCGTGAAC TTCCCCGCCG ACGGCCCCGT 4561 GATGAAGAAG ATGACCGACA ACTGGGAGCC CTCCTGCGAG AAGATCATCC CCGTGCCCAA 4621 GCAGGGCATC TTGAAGGGCG ACGTGAGCAT GTACCTGCTG CTGAAGGACG GTGGCCGCTT 4681 GCGCTGCCAG TTCGACACCG TGTACAAGGC CAAGTCCGTG CCCCGCAAGA TGCCCGACTG 4741 GCACTTCATC CAGCACAAGC TGACCCGCGA GGACCGCAGC GACGCCAAGA ACCAGAAGTG 4801 GCACCTGACC GAGCACGCCA TCGCCTCCGG CTCCGCCTTG CCCgcggccg ctgagggcag 4861 aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatga ccgagtacaa 4921 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc 4981 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg 5041 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg 5101 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg 5161 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc 5221 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct 5281 ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct 5341 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc 5401 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc 5461 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaaatc aacctctgga 5521 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg 5581 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt 5641 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag 5701 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc 5761 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga 5821 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa 5881 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac 5941 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct 6001 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc ttcgccctca 6061 gacgagtcgg atctcccttt ggccgcctcc ccgcctggta cctttaagac caatgactta 6121 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 6181 tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca 6241 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 6301 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 6361 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 6421 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 6481 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 6541 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 6601 ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc 6661 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag 6721 aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa 6781 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 6841 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 6901 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 6961 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 7021 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 7081 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 7141 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 7201 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 7261 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 7321 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 7381 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 7441 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 7501 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 7561 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 7621 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 7681 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 7741 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 7801 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 7861 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 7921 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 7981 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 8041 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 8101 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 8161 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 8221 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 8281 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 8341 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 8401 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 8461 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 8521 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 8581 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 8641 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 8701 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 8761 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 8821 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 8881 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 8941 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 9001 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag 9061 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca 9121 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg 9181 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 9241 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct 9301 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg 9361 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.