pLV3-CMV-KLF4-3×FLAG-EF1a-CopG

pLV3-CMV-KLF4-3×FLAG-EF1a-CopGFP-Neo plasmid

PVT94703

pLV3-CMV-KLF4-3×FLAG-EF1a-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-KLF4(human)-3×FLAG-EF1a-CopGFP-Neo
Alias: NM_001314052.2;EZF; GKLF
Gene lenght: 1539bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-KLF4-3×FLAG-EF1a-CopGFP-Neo plasmid Description

pLV3-CMV-KLF4-3×FLAG-EF1a-CopGFP-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-KLF4-3×FLAG-EF1a-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgaggca gccacctggc gagtctgaca tggctgtcag 2341 cgacgcgctg ctcccatctt tctccacgtt cgcgtctggc ccggcgggaa gggagaagac 2401 actgcgtcaa gcaggtgccc cgaataaccg ctggcgggag gagctctccc acatgaagcg 2461 acttccccca gtgcttcccg gccgccccta tgacctggcg gcggcgaccg tggccacaga 2521 cctggagagc ggcggagccg gtgcggcttg cggcggtagc aacctggcgc ccctacctcg 2581 gagagagacc gaggagttca acgatctcct ggacctggac tttattctct ccaattcgct 2641 gacccatcct ccggagtcag tggccgccac cgtgtcctcg tcagcgtcag cctcctcttc 2701 gtcgtcgccg tcgagcagcg gccctgccag cgcgccctcc acctgcagct tcacctatcc 2761 gatccgggcc gggaacgacc cgggcgtggc gccgggcggc acgggcggag gcctcctcta 2821 tggcagggag tccgctcccc ctccgacggc tcccttcaac ctggcggaca tcaacgacgt 2881 gagcccctcg ggcggcttcg tggccgagct cctgcggcca gaattggacc cggtgtacat 2941 tccgccgcag cagccgcagc cgccaggtgg cgggctgatg ggcaagttcg tgctgaaggc 3001 gtcgctgagc gcccctggca gcgagtacgg cagcccgtcg gtcatcagcg tcagcaaagg 3061 cagccctgac ggcagccacc cggtggtggt ggcgccctac aacggcgggc cgccgcgcac 3121 gtgccccaag atcaagcagg aggcggtctc ttcgtgcacc cacttgggcg ctggaccccc 3181 tctcagcaat ggccaccggc cggctgcaca cgacttcccc ctggggcggc agctccccag 3241 caggactacc ccgaccctgg gtcttgagga agtgctgagc agcagggact gtcaccctgc 3301 cctgccgctt cctcccggct tccatcccca cccggggccc aattacccat ccttcctgcc 3361 cgatcagatg cagccgcaag tcccgccgct ccattaccaa ggtcagtccc ggggatttgt 3421 agctcgggct ggggagccct gtgtgtgctg gccccacttc gggacacacg ggatgatgct 3481 caccccacct tcttcacccc tagagctcat gccacccggt tcctgcatgc cagaggagcc 3541 caagccaaag aggggaagac gatcgtggcc ccggaaaagg accgccaccc acacttgtga 3601 ttacgcgggc tgcggcaaaa cctacacaaa gagttcccat ctcaaggcac acctgcgaac 3661 ccacacaggt gagaaacctt accactgtga ctgggacggc tgtggatgga aattcgcccg 3721 ctcagatgaa ctgaccaggc actaccgtaa acacacgggg caccgcccgt tccagtgcca 3781 aaaatgcgac cgagcatttt ccaggtcgga ccacctcgcc ttacacatga agaggcattt 3841 tggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA 3901 TGACGATGAC AAGTAgcggc cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc 3961 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg 4021 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct 4081 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt 4141 tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc 4201 acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc 4261 tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc 4321 gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac 4381 gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg 4441 ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag 4501 cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt 4561 cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa 4621 gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg 4681 cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa 4741 caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt 4801 gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac 4861 cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt 4921 ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag 4981 cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc 5041 catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga 5101 gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat 5161 cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc 5221 cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga 5281 gaatcccggc cctGCCACCa tggctgaaca agatggattg cacgcaggtt ctccggccgc 5341 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 5401 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 5461 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg 5521 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 5581 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 5641 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 5701 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 5761 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 5821 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 5881 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 5941 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 6001 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 6061 cgccttctat cgccttcttg acgagttctt ctgaGTCGAC AATCAACCTC TGGATTACAA 6121 AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA 6181 CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC 6241 CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG 6301 TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC 6361 CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT 6421 CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT 6481 GGTGTTGTCG GGGAAATCAT CGTCCTTTCC TTGGCTGCTC GCCTGTGTTG CCACCTGGAT 6541 TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC 6601 CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG 6661 TCGGATCTCC CTTTGGGCCG CCTCCCCGCg gtacctttaa gaccaatgac ttacaaggca 6721 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 6781 caacgaaGat aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga 6841 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 6901 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 6961 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 7021 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca 7081 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 7141 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc 7201 tagctatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 7261 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 7321 gaggaggctt ttttggaggc ctagactttt gcagagacgg cccaaattcg taatcatggt 7381 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 7441 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 7501 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 7561 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 7621 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 7681 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 7741 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 7801 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 7861 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 7921 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 7981 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 8041 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 8101 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 8161 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 8221 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 8281 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 8341 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 8401 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 8461 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 8521 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 8581 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 8641 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 8701 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 8761 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 8821 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 8881 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 8941 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 9001 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 9061 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 9121 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 9181 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 9241 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 9301 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 9361 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 9421 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 9481 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag 9541 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 9601 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 9661 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 9721 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 9781 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 9841 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 9901 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 9961 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.