pLV3-CMV-SIRT2-6×His-Hygro pla

pLV3-CMV-SIRT2-6×His-Hygro plasmid

PVT94745

pLV3-CMV-SIRT2-6×His-Hygro plasmid Information

Plasmid name: pLV3-CMV-SIRT2(human)-6×His-Hygro
Alias: NM_012237.4;SIR2; SIR2L; SIR2L2
Gene lenght: 1167bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Hyg
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SIRT2-6×His-Hygro plasmid Description

pLV3-CMV-SIRT2-6×His-Hygro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-SIRT2-6×His-Hygro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt 1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca 1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc 2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga 2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat 2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag 2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct 2281 agaGCCACCa tggcagagcc agacccctct caccctctgg agacccaggc agggaaggtg 2341 caggaggctc aggactcaga ttcagactct gagggaggag ccgctggtgg agaagcagac 2401 atggacttcc tgcggaactt attctcccag acgctcagcc tgggcagcca gaaggagcgt 2461 ctgctggacg agctgacctt ggaaggggtg gcccggtaca tgcagagcga acgctgtcgc 2521 agagtcatct gtttggtggg agctggaatc tccacatccg caggcatccc cgactttcgc 2581 tctccatcca ccggcctcta tgacaaccta gagaagtacc atcttcccta cccagaggcc 2641 atctttgaga tcagctattt caagaaacat ccggaaccct tcttcgccct cgccaaggaa 2701 ctctatcctg ggcagttcaa gccaaccatc tgtcactact tcatgcgcct gctgaaggac 2761 aaggggctac tcctgcgctg ctacacgcag aacatagata ccctggagcg aatagccggg 2821 ctggaacagg aggacttggt ggaggcgcac ggcaccttct acacatcaca ctgcgtcagc 2881 gccagctgcc ggcacgaata cccgctaagc tggatgaaag agaagatctt ctctgaggtg 2941 acgcccaagt gtgaagactg tcagagcctg gtgaagcctg atatcgtctt ttttggtgag 3001 agcctcccag cgcgtttctt ctcctgtatg cagtcagact tcctgaaggt ggacctcctc 3061 ctggtcatgg gtacctcctt gcaggtgcag ccctttgcct ccctcatcag caaggcaccc 3121 ctctccaccc ctcgcctgct catcaacaag gagaaagctg gccagtcgga ccctttcctg 3181 gggatgatta tgggcctcgg aggaggcatg gactttgact ccaagaaggc ctacagggac 3241 gtggcctggc tgggtgaatg cgaccagggc tgcctggccc ttgctgagct ccttggatgg 3301 aagaaggagc tggaggacct tgtccggagg gagcacgcca gcatagatgc ccagtcgggg 3361 gcgggggtcc ccaaccccag cacttcagct tcccccaaga agtccccgcc acctgccaag 3421 gacgaggcca ggacaacaga gagggagaaa ccccagCATC ACCATCATCA CCATTGAgga 3481 tccgcggccg caaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 3541 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 3601 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 3661 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 3721 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 3781 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 3841 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 3901 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 3961 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 4021 ctgtgaccgg cgcctacgtc gacatgaaaa agcctgaact caccgcgacg tctgtcgaga 4081 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag 4141 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct 4201 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc 4261 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc 4321 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc 4381 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt 4441 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg 4501 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt 4561 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc 4621 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag 4681 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct 4741 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc 4801 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc 4861 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat 4921 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa 4981 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc 5041 ccagcactcg tccgagggca aaggaatagg tcgacaatca acctctggat tacaaaattt 5101 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 5161 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 5221 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 5281 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 5341 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 5401 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 5461 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc 5521 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 5581 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 5641 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct 5701 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa 5761 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc 5821 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 5881 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 5941 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag 6001 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct 6061 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca 6121 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag 6181 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 6241 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 6301 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat 6361 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 6421 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 6481 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 6541 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 6601 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 6661 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 6721 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 6781 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 6841 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 6901 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 6961 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 7021 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 7081 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 7141 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 7201 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 7261 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 7321 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 7381 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 7441 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 7501 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 7561 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 7621 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 7681 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 7741 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 7801 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 7861 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 7921 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 7981 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 8041 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 8101 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 8161 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 8221 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 8281 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 8341 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 8401 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 8461 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa 8521 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg 8581 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag 8641 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg 8701 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc 8761 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt 8821 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac 8881 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt 8941 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.