PiggyBac-TRE3GS-PRDM16-PGK-Tet

PiggyBac-TRE3GS-PRDM16-PGK-TetOn-EF1a-Puro plasmid

PVT96419

PiggyBac-TRE3GS-PRDM16-PGK-TetOn-EF1a-Puro plasmid Information

Plasmid name: PiggyBac-TRE3GS-PRDM16(human)-PGK-TetOn-EF1a-Puro
Alias: NM_022114.4;CMD1LL; KMT8F; LVNC8; MEL1; PFM13
Gene lenght: 3831bp
Host: Mammalian cells
Use(s): Protein Expression,Tetracycline induction,Transposition
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: TRE3GS
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: TRE3GS-F1
Reverse primer:
Copy number: High
Induction:

PiggyBac-TRE3GS-PRDM16-PGK-TetOn-EF1a-Puro plasmid Description

PiggyBac-TRE3GS-PRDM16-PGK-TetOn-EF1a-Puro is a plasmid suitable for protein expression, transposition, and tetracycline induction in mammalian cells.

PiggyBac-TRE3GS-PRDM16-PGK-TetOn-EF1a-Puro plasmid Sequence

1 ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA 61 CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA 121 AGTGCCACCT AAATTGTAAG CGTTAATATT TTGTTAAAAT TCGCGTTAAA TTTTTGTTAA 181 ATCAGCTCAT TTTTTAACCA ATAGGCCGAA ATCGGCAAAA TCCCTTATAA ATCAAAAGAA 241 TAGACCGAGA TAGGGTTGAG TGTTGTTCCA GTTTGGAACA AGAGTCCACT ATTAAAGAAC 301 GTGGACTCCA ACGTCAAAGG GCGAAAAACC GTCTATCAGG GCGATGGCCC ACTACGTGAA 361 CCATCACCCT AATCAAGTTT TTTGGGGTCG AGGTGCCGTA AAGCACTAAA TCGGAACCCT 421 AAAGGGAGCC CCCGATTTAG AGCTTGACGG GGAAAGCCGG CGAACGTGGC GAGAAAGGAA 481 GGGAAGAAAG CGAAAGGAGC GGGCGCTAGG GCGCTGGCAA GTGTAGCGGT CACGCTGCGC 541 GTAACCACCA CACCCGCCGC GCTTAATGCG CCGCTACAGG GCGCGTCCCA TTCGCCATTC 601 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG 661 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA 721 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCCTCGTTCA TTCACGTTTT TGAACCCGTG 781 GAGGACGGGC AGACTCGCGG TGCAAATGTG TTTTACAGCG TGATGGAGCA GATGAAGATG 841 CTCGACACGC TGCAGAACAC GCAGCTAGAT TAACCCTAGA AAGATAATCA TATTGTGACG 901 TACGTTAAAG ATAATCATGT GTAAAATTGA CGCATGTGTT TTATCGGTCT GTATATCGAG 961 GTTTATTTAT TAATTTGAAT AGATATTAAG TTTTATTATA TTTACACTTA CATACTAATA 1021 ATAAATTCAA CAAACAATTT ATTTATGTTT ATTTATTTAT TAAAAAAAAC AAAAACTCAA 1081 AATTTCTTCT ATAAAGTAAC AAAACTTTTA TGAGGGACAG CCCCCCCCCA AAGCCCCCAG 1141 GGATGTAATT ACGTCCCTCC CCCGCTAGGG GGCAGCAGCG AGCCGCCCGG GGCTCCGCTC 1201 CGGTCCGGCG CTCCCCCCGC ATCCCCGAGC CGGCAGCGTG CGGGGACAGC CCGGGCACGG 1261 GGAAGGTGGC ACGGGATCGC TTTCCTCTGA ACGCTTCTCG CTGCTCTTTG AGCCTGCAGA 1321 CACCTGGGGG GATACGGGGA AAAGGCCTCC AcGGCCACTA GTATTATGCC CAGTACATGA 1381 CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCaatt 1441 atcacctcga gtttactccc tatcagtgat agagaacgta tgaagagttt actccctatc 1501 agtgatagag aacgtatgca gactttactc cctatcagtg atagagaacg tataaggagt 1561 ttactcccta tcagtgatag agaacgtatg accagtttac tccctatcag tgatagagaa 1621 cgtatctaca gtttactccc tatcagtgat agagaacgta tatccagttt actccctatc 1681 agtgatagag aacgtataag ctttgcttat gtaaaccagg gcgcctataa aagagtgctg 1741 attttttgag taaacttcaa ttccacaaca cttttgtctt ataccaactt tccgtaccac 1801 ttcctaccct cgtaaagaat tcccgtatac accggtgcca ccatgcgatc caaggcgagg 1861 gcgaggaagc tagccaaaag tgacggtgac gttgtaaata atatgtatga gcccaaccgg 1921 gacctgctgg ccagccacag cgcggaggac gaggccgagg acagtgccat gtcgcccatc 1981 cccgtggggc caccgtcccc cttccccacc agcgaggact tcacccccaa ggagggctcg 2041 ccgtacgagg cccctgtcta cattcctgaa gacattccga tcccagcaga cttcgagctc 2101 cgagagtcct ccatcccagg ggctggcctg ggggtctggg ccaagaggaa gatggaagcc 2161 ggggagaggc tgggcccctg cgtggtggtg ccccgggcgg cggcaaagga gacagacttc 2221 ggatgggagc aaatactgac ggacgtggaa gtgtcgcccc aggaaggctg catcacaaag 2281 atctccgaag acctgggcag tgagaagttc tgcgtggatg caaatcaggc gggggctggc 2341 agctggctca agtacatccg tgtggcgtgc tcctgcgatg accagaacct caccatgtgt 2401 cagatcagtg agcagattta ctataaagtc attaaggaca ttgagccagg tgaggagctg 2461 ctggtgcacg tgaaggaagg cgtctacccc ctgggcacag tgccgcccgg cctggacgag 2521 gagcccacgt tccgctgtga cgagtgtgac gaactcttcc agtccaagct ggacctgcgg 2581 cgccataaga agtacacgtg tggctcagtg ggggctgcgc tctacgaggg cctggctgag 2641 gagctcaagc ccgagggcct tggcggtggc agcggccaag cccacgagtg caaggactgc 2701 gagcggatgt tccccaacaa gtacagcctg gagcagcaca tggtcatcca cacggaggag 2761 cgcgagtaca aatgcgacca gtgtcccaag gccttcaact ggaagtccaa cctcatccgc 2821 caccagatgt cccacgacag cggcaaacgc ttcgaatgtg aaaactgcgt gaaggtgttc 2881 acggacccca gcaaccttca gcggcacatc cgctcgcagc acgtgggcgc tcgggcccac 2941 gcctgccccg actgcgggaa gaccttcgcc acgtcctccg gcctcaagca gcacaagcat 3001 atccacagca cggtgaagcc tttcatatgt gaggtctgcc acaagtccta cacgcagttc 3061 tccaacctgt gccggcacaa gcggatgcac gccgactgcc gcacgcagat caagtgcaag 3121 gactgtggcc agatgttcag cactacctcc tccctcaaca agcaccggcg cttctgcgag 3181 ggcaagaacc attacacgcc gggcggcatc tttgccccgg gcctgccctt gacccccagc 3241 cccatgatgg acaaggcaaa accctccccc agcctcaatc acgccagcct gggcttcaac 3301 gagtactttc cctccaggcc gcacccgggg agcctgccct tctccacggc gcctcccacg 3361 ttccccgcac tcacccccgg cttcccgggc atcttccctc catccttgta cccccggccg 3421 cctctgctac ctcccacatc gctgctcaag agccccctga accacaccca ggacgccaag 3481 ctccccagtc ccctggggaa cccagccctg cccctggtct ccgccgtcag caacagcagc 3541 cagggcacga cggcagctgc ggggcccgag gagaagttcg agagccgcct ggaggactcc 3601 tgtgtggaga agctgaagac caggagcagc gacatgtcgg acggcagtga ctttgaggac 3661 gtcaacacca ccacggggac cgacctggac acgaccacgg ggacgggctc ggacctggac 3721 agcgacgtgg acagcgaccc tgacaaggac aagggcaagg gcaagtccgc cgagggccag 3781 cccaagtttg ggggcggctt ggcgcccccg ggggccccga acagcgtggc cgaggtgcct 3841 gtcttctatt cccagcactc attcttcccg ccacccgacg agcagctgct gactgcaacg 3901 ggcgccgccg gggactccat caaggccatc gcatccattg ccgagaagta ctttggcccc 3961 ggcttcatgg ggatgcagga gaagaagctg ggctcgctcc cctaccactc ggcgttcccc 4021 ttccagttcc tgcccaactt cccccactcc ctttacccct tcacggaccg agccctcgcc 4081 cacaacttgc tggtcaaggc cgagccaaag tcaccccggg acgccctcaa ggtgggcggc 4141 cccagtgccg agtgcccctt tgatctcacc accaagccca aagacgtgaa gcccatcctg 4201 cccatgccca agggcccctc ggcccccgca tccggcgagg agcagccgct ggacctgagc 4261 atcggcagcc gggcccgtgc cagccaaaac ggcggcgggc gggagccccg caagaaccac 4321 gtctatgggg aacgcaagct gggcgccggc gaggggctgc cccaggtgtg cccggcgcgg 4381 atgccccagc agcccccgct ccactacgcc aagccctcgc ccttcttcat ggaccccatc 4441 tacagcaggg tagaaaagcg gaaggtcaca gaccccgtgg gagccctgaa ggagaagtac 4501 ctgcggccgt ccccgctgct cttccacccc cagatgtcag ccatagagac catgacagag 4561 aagctggaga gctttgcagc catgaaggcg gactcgggca gctccctgca gcccctcccc 4621 caccacccct tcaacttccg gtccccaccc ccaacgctct ccgaccccat cctcaggaag 4681 ggcaaggagc gatacacgtg caggtactgt gggaagatct tccccagatc agccaatctc 4741 accagacacc tgaggacgca cactggggag cagccgtaca ggtgtaagta ctgcgaccgc 4801 tccttcagca tctcttcgaa cctccagcgg cacgtccgga acatccacaa caaggagaag 4861 cctttcaagt gccacctgtg caaccgctgc ttcgggcagc agaccaacct ggaccggcac 4921 ctcaagaagc acgagcacga gaacgcacca gtgagccagc accccggggt cctcacgaac 4981 cacctgggga ccagcgcgtc ctctcccacc tcagagtcgg acaaccacgc acttttagac 5041 gagaaagaag actcttattt ctcggaaatc agaaacttta ttgccaatag tgagatgaac 5101 caagcatcaa cgcgaacaga gaaacgggcg gacatgcaga tcgtggacgg cagtgcccag 5161 tgtccaggcc tagccagtga gaagcaggag gacgtggagg aggaggacga cgatgacctg 5221 gaggaggacg atgaggacag cctggccggg aagtcgcagg atgacaccgt gtcccccgca 5281 cccgagcccc aggccgccta cgaggatgag gaggatgagg agccagccgc ctccctggcc 5341 gtgggctttg accacacccg aaggtgtgct gaggaccacg aaggcggtct gttagctttg 5401 gagccgatgc cgacttttgg gaaggggctg gacctccgca gagcagctga ggaagcattt 5461 gaagttaaag atgtgcttaa ttccacctta gattctgagg ctttaaaaca tacactgtgc 5521 aggcaggcta agaaccaggc atatgcaatg atgctgtccc tttccgaaga cactcctctc 5581 cacaccccct cccagggttc tctggacgct tggttgaagg tcactggagc cacgtcggag 5641 tctggagcat ttcaccccat caaccacctc tgaggatcca CTCgaggtga taattccacg 5701 gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct 5761 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc 5821 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct 5881 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg 5941 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg 6001 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc 6061 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg 6121 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc 6181 gttgaccgaa tcaccgacct ctctccccag ggggatcatc gaattaccat gtctagactg 6241 gacaagagca aagtcataaa ctctgctctg gaattactca atggagtcgg tatcgaaggc 6301 ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct gtactggcac 6361 gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga caggcatcat 6421 acccactcct gccccctgga aggcgagtca tggcaagact ttctgcggaa caacgccaag 6481 tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct cggcacccgc 6541 ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc 6601 ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac actgggctgc 6661 gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc taccaccgat 6721 tctatgcccc cacttctgaa acaagcaatt gagctgttcg accggcaggg agccgaacct 6781 gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct aaagtgcgaa 6841 agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc agccgatgcc 6901 cttgacgact ttgaccttga tatgctgcct gctgacgctc ttgacgattt tgaccttgac 6961 atgctccccg ggtaaGCGGC CGCGAAGGAT CTGCGATCGC TCCGGTGCCC GTCAGTGGGC 7021 AGAGCGCACA TCGCCCACAG TCCCCGAGAA GTTGGGGGGA GGGGTCGGCA ATTGAACGGG 7081 TGCCTAGAGA AGGTGGCGCG GGGTAAACTG GGAAAGTGAT GTCGTGTACT GGCTCCGCCT 7141 TTTTCCCGAG GGTGGGGGAG AACCGTATAT AAGTGCAGTA GTCGCCGTGA ACGTTCTTTT 7201 TCGCAACGGG TTTGCCGCCA GAACACAGCT GAAGCTTCGA GGGGCTCGCA TCTCTCCTTC 7261 ACGCGCCCGC CGCCCTACCT GAGGCCGCCA TCCACGCCGG TTGAGTCGCG TTCTGCCGCC 7321 TCCCGCCTGT GGTGCCTCCT GAACTGCGTC CGCCGTCTAG GTAAGTTTAA AGCTCAGGTC 7381 GAGACCGGGC CTTTGTCCGG CGCTCCCTTG GAGCCTACCT AGACTCAGCC GGCTCTCCAC 7441 GCTTTGCCTG ACCCTGCTTG CTCAACTCTA CGTCTTTGTT TCGTTTTCTG TTCTGCGCCG 7501 TTACAGATCC AAGCTGTGAC CGGCGCCTAC GCTAGACGCC ACCATGACCG AGTACAAGCC 7561 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC 7621 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT 7681 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT 7741 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC 7801 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA 7861 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC 7921 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC 7981 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG 8041 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA 8101 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGAAATCAAC CTCTGGATTA 8161 CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG 8221 ATACGCTGCT TTAATGCCTT TGTATCAGTT AACTTGTTTA TTGCAGCTTA TAATGGTTAC 8281 AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT 8341 TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GGAATTGACT CAAATGATGT 8401 CAATTAGTCT ATCAGAAGCT ATCTGGTCTC CCTTCCGGGG GACAAGACAT CCCTGTTTAA 8461 TATTTAAACA GCAGTGTTCC CAAACTGGGT TCTTATATCC CTTGCTCTGG TCAACCAGGT 8521 TGCAGGGTTT CCTGTCCTCA CAGGAACGAA GTCCCTAAAG AAACAGTGGC AGCCAGGTTT 8581 AGCCCCGGAA TTGACTGGAT TCCTTTTTTA GGGCCCATTG GTATGGCTTT TTCCCCGTAT 8641 CCCCCCAGGT GTCTGCAGGC TCAAAGAGCA GCGAGAAGCG TTCAGAGGAA AGCGATCCCG 8701 TGCCACCTTC CCCGTGCCCG GGCTGTCCCC GCACGCTGCC GGCTCGGGGA TGCGGGGGGA 8761 GCGCCGGACC GGAGCGGAGC CCCGGGCGGC TCGCTGCTGC CCCCTAGCGG GGGAGGGACG 8821 TAATTACATC CCTGGGGGCT TTGGGGGGGG GCTGTCCCTG ATATCTATAA CAAGAAAATA 8881 TATATATAAT AAGTTATCAC GTAAGTAGAA CATGAAATAA CAATATAATT ATCGTATGAG 8941 TTAAATCTTA AAAGTCACGT AAAAGATAAT CATGCGTCAT TTTGACTCAC GCGGTCGTTA 9001 TAGTTCAAAA TCAGTGACAC TTACCGCATT GACAAGCACG CCTCACGGGA GCTCCAAGCG 9061 GCGACTGAGA TGTCCTAAAT GCACAGCGAC GGATTCGCGC TATTTAGAAA GAGAGAGCAA 9121 TATTTCAAGA ATGCATGCGT CAATTTTACG CAGACTATCT TTCTAGGGTT AATCTAGCTG 9181 CATCAGGATC ATATCGTCGG GTCTTTTTTC CGGCTCAGTC ATCGCCCAAG CTGGCGCTAT 9241 CTGGGCATCG GGGAGGAAGA AGCCCGTGCC TTTTCCCGCG AGGTTGAAGC GGCATGGAAA 9301 GAGTTTGCCG AGGATGACTG CTGCTGCATT GACGTTGAGC GAAAACGCAC GTTTACCATG 9361 ATGATTCGGG AAGGTGTGGC CATGCACGCC TTTAACGGTG AACTGTTCGT TCAGGCCACC 9421 TGGGATACCA GTTCGTCGCG GCTTTTCCGG ACACAGTTCC GGATGGTCAG CCCGAAGCGC 9481 ATCAGCAACC CGAACAATAC CGGCGACAGC CGGAACTGCC GTGCCGGTGT GCAGATTAAT 9541 GACAGCGGTG CGGCGCTGGG ATATTACGTC AGCGAGGACG GGTATCCTGG CTGGATGCCG 9601 CAGAAATGGA CATGGATACC CCGTGAGTTA CCCGGCGGGC GCGCTTGGCG TAATCATGGT 9661 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 9721 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 9781 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 9841 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 9901 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 9961 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 10021 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 10081 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 10141 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 10201 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT 10261 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 10321 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 10381 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 10441 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 10501 GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 10561 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC 10621 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 10681 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 10741 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 10801 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 10861 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 10921 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 10981 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 11041 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 11101 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 11161 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 11221 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 11281 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 11341 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 11401 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 11461 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 11521 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 11581 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 11641 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.