pSV40-Rluc-Slc2a1-3UTR-Fluc pl

pSV40-Rluc-Slc2a1-3UTR-Fluc plasmid

PVT95505

pSV40-Rluc-Slc2a1-3UTR-Fluc plasmid Information

Plasmid name: pSV40-Rluc-Slc2a1(rat)-3UTR-Fluc
Alias:
Gene lenght: 210bp
Host: Mammalian cells
Use(s): Double luciferase reporter
Fragment type: UTR
Species: Rat
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc,Rluc
Promoter: SV40
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: psiCHECK-2-F
Reverse primer:
Copy number: High
Induction:

pSV40-Rluc-Slc2a1-3UTR-Fluc plasmid Description

pSV40-Rluc-Slc2a1-3UTR-Fluc is a plasmid suitable for dual-luciferase reporting in mammalian cells.

pSV40-Rluc-Slc2a1-3UTR-Fluc plasmid Sequence

1 AGATCTGCGC AGCACCATGG CCTGAAATAA CCTCTGAAAG AGGAACTTGG TTAGGTACCT 61 TCTGAGGCGG AAAGAACCAG CTGTGGAATG TGTGTCAGTT AGGGTGTGGA AAGTCCCCAG 121 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCAGGTGTG 181 GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG 241 CAACCATAGT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC 301 ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC AGAGGCCGAG GCCGCCTCGG 361 CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA 421 AGCTTGATTC TTCTGACACA ACAGTCTCGA ACTTAAGCTG CAGAAGTTGG TCGTGAGGCA 481 CTGGGCAGGT AAGTATCAAG GTTACAAGAC AGGTTTAAGG AGACCAATAG AAACTGGGCT 541 TGTCGAGACA GAGAAGACTC TTGCGTTTCT GATAGGCACC TATTGGTCTT ACTGACATCC 601 ACTTTGCCTT TCTCTCCACA GGTGTCCACT CCCAGTTCAA TTACAGCTCT TAAGGCTAGA 661 GTACTTAATA CGACTCACTA TAGGCTAGCC ACCATGGCTT CCAAGGTGTA CGACCCCGAG 721 CAACGCAAAC GCATGATCAC TGGGCCTCAG TGGTGGGCTC GCTGCAAGCA AATGAACGTG 781 CTGGACTCCT TCATCAACTA CTATGATTCC GAGAAGCACG CCGAGAACGC CGTGATTTTT 841 CTGCATGGTA ACGCTGCCTC CAGCTACCTG TGGAGGCACG TCGTGCCTCA CATCGAGCCC 901 GTGGCTAGAT GCATCATCCC TGATCTGATC GGAATGGGTA AGTCCGGCAA GAGCGGGAAT 961 GGCTCATATC GCCTCCTGGA TCACTACAAG TACCTCACCG CTTGGTTCGA GCTGCTGAAC 1021 CTTCCAAAGA AAATCATCTT TGTGGGCCAC GACTGGGGGG CTTGTCTGGC CTTTCACTAC 1081 TCCTACGAGC ACCAAGACAA GATCAAGGCC ATCGTCCATG CTGAGAGTGT CGTGGACGTG 1141 ATCGAGTCCT GGGACGAGTG GCCTGACATC GAGGAGGATA TCGCCCTGAT CAAGAGCGAA 1201 GAGGGCGAGA AAATGGTGCT TGAGAATAAC TTCTTCGTCG AGACCATGCT CCCAAGCAAG 1261 ATCATGCGGA AACTGGAGCC TGAGGAGTTC GCTGCCTACC TGGAGCCATT CAAGGAGAAG 1321 GGCGAGGTTA GACGGCCTAC CCTCTCCTGG CCTCGCGAGA TCCCTCTCGT TAAGGGAGGC 1381 AAGCCCGACG TCGTCCAGAT TGTCCGCAAC TACAACGCCT ACCTTCGGGC CAGCGACGAT 1441 CTGCCTAAGA TGTTCATCGA GTCCGACCCT GGGTTCTTTT CCAACGCTAT TGTCGAGGGA 1501 GCTAAGAAGT TCCCTAACAC CGAGTTCGTG AAGGTGAAGG GCCTCCACTT CAGCCAGGAG 1561 GACGCTCCAG ATGAAATGGG TAAGTACATC AAGAGCTTCG TGGAGCGCGT GCTGAAGAAC 1621 GAGCAGTAAT TCTAGGCGAT CGCTCGAGGG AGCCCACAGC CAGCCCCGCC TGCTCCCAGC 1681 AGCCCCGAGG ATCTCTCTGG AGCACAGGCA GCTAGATGAG ACCTCTTCCA AACTGACAGA 1741 TCTCGGGAGA GCCGGGCCTG GGCACCTTTC TTCAGTCAGC AATGAAGTCC AGAAGAATAT 1801 TCAGGACTTT GATGGCTCCA GAATTTTTAA TGAAAGCAAG ACTGTTGCTC AGATCTATGC 1861 GGCCGCTGGC CGCAATAAAA TATCTTTATT TTCATTACAT CTGTGTGTTG GTTTTTTGTG 1921 TGAGGATCTA AATGAGTCTT CGGACCTCGC GGGGGCCGCT TAAGCGGTGG TTAGGGTTTG 1981 TCTGACGCGG GGGGAGGGGG AAGGAACGAA ACACTCTCAT TCGGAGGCGG CTCGGGGTTT 2041 GGTCTTGGTG GCCACGGGCA CGCAGAAGAG CGCCGCGATC CTCTTAAGCA CCCCCCCGCC 2101 CTCCGTGGAG GCGGGGGTTT GGTCGGCGGG TGGTAACTGG CGGGCCGCTG ACTCGGGCGG 2161 GTCGCGCGCC CCAGAGTGTG ACCTTTTCGG TCTGCTCGCA GACCCCCGGG CGGCGCCGCC 2221 GCGGCGGCGA CGGGCTCGCT GGGTCCTAGG CTCCATGGGG ACCGTATACG TGGACAGGCT 2281 CTGGAGCATC CGCACGACTG CGGTGATATT ACCGGAGACC TTCTGCGGGA CGAGCCGGGT 2341 CACGCGGCTG ACGCGGAGCG TCCGTTGGGC GACAAACACC AGGACGGGGC ACAGGTACAC 2401 TATCTTGTCA CCCGGAGGCG CGAGGGACTG CAGGAGCTTC AGGGAGTGGC GCAGCTGCTT 2461 CATCCCCGTG GCCCGTTGCT CGCGTTTGCT GGCGGTGTCC CCGGAAGAAA TATATTTGCA 2521 TGTCTTTAGT TCTATGATGA CACAAACCCC GCCCAGCGTC TTGTCATTGG CGAATTCGAA 2581 CACGCAGATG CAGTCGGGGC GGCGCGGTCC CAGGTCCACT TCGCATATTA AGGTGACGCG 2641 TGTGGCCTCG AACACCGAGC GACCCTGCAG CGACCCGCTT AAAAGCTTGG CATTCCGGTA 2701 CTGTTGGTAA AGCCACCATG GCCGATGCTA AGAACATTAA GAAGGGCCCT GCTCCCTTCT 2761 ACCCTCTGGA GGATGGCACC GCTGGCGAGC AGCTGCACAA GGCCATGAAG AGGTATGCCC 2821 TGGTGCCTGG CACCATTGCC TTCACCGATG CCCACATTGA GGTGGACATC ACCTATGCCG 2881 AGTACTTCGA GATGTCTGTG CGCCTGGCCG AGGCCATGAA GAGGTACGGC CTGAACACCA 2941 ACCACCGCAT CGTGGTGTGC TCTGAGAACT CTCTGCAGTT CTTCATGCCA GTGCTGGGCG 3001 CCCTGTTCAT CGGAGTGGCC GTGGCCCCTG CTAACGACAT TTACAACGAG CGCGAGCTGC 3061 TGAACAGCAT GGGCATTTCT CAGCCTACCG TGGTGTTCGT GTCTAAGAAG GGCCTGCAGA 3121 AGATCCTGAA CGTGCAGAAG AAGCTGCCTA TCATCCAGAA GATCATCATC ATGGACTCTA 3181 AGACCGACTA CCAGGGCTTC CAGAGCATGT ACACATTCGT GACATCTCAT CTGCCTCCTG 3241 GCTTCAACGA GTACGACTTC GTGCCAGAGT CTTTCGACAG GGACAAAACC ATTGCCCTGA 3301 TCATGAACAG CTCTGGGTCT ACCGGCCTGC CTAAGGGCGT GGCCCTGCCT CATCGCACCG 3361 CCTGTGTGCG CTTCTCTCAC GCCCGCGACC CTATTTTCGG CAACCAGATC ATCCCCGACA 3421 CCGCTATTCT GAGCGTGGTG CCATTCCACC ACGGCTTCGG CATGTTCACC ACCCTGGGCT 3481 ACCTGATTTG CGGCTTTCGG GTGGTGCTGA TGTACCGCTT CGAGGAGGAG CTGTTCCTGC 3541 GCAGCCTGCA AGACTACAAA ATTCAGTCTG CCCTGCTGGT GCCAACCCTG TTCAGCTTCT 3601 TCGCTAAGAG CACCCTGATC GACAAGTACG ACCTGTCTAA CCTGCACGAG ATTGCCTCTG 3661 GCGGCGCCCC ACTGTCTAAG GAGGTGGGCG AAGCCGTGGC CAAGCGCTTT CATCTGCCAG 3721 GCATCCGCCA GGGCTACGGC CTGACCGAGA CAACCAGCGC CATTCTGATT ACCCCAGAGG 3781 GCGACGACAA GCCTGGCGCC GTGGGCAAGG TGGTGCCATT CTTCGAGGCC AAGGTGGTGG 3841 ACCTGGACAC CGGCAAGACC CTGGGAGTGA ACCAGCGCGG CGAGCTGTGT GTGCGCGGCC 3901 CTATGATTAT GTCCGGCTAC GTGAATAACC CTGAGGCCAC AAACGCCCTG ATCGACAAGG 3961 ACGGCTGGCT GCACTCTGGC GACATTGCCT ACTGGGACGA GGACGAGCAC TTCTTCATCG 4021 TGGACCGCCT GAAGTCTCTG ATCAAGTACA AGGGCTACCA GGTGGCCCCA GCCGAGCTGG 4081 AGTCTATCCT GCTGCAGCAC CCTAACATTT TCGACGCCGG AGTGGCCGGC CTGCCCGACG 4141 ACGATGCCGG CGAGCTGCCT GCCGCCGTCG TCGTGCTGGA ACACGGCAAG ACCATGACCG 4201 AGAAGGAGAT CGTGGACTAT GTGGCCAGCC AGGTGACAAC CGCCAAGAAG CTGCGCGGCG 4261 GAGTGGTGTT CGTGGACGAG GTGCCCAAGG GCCTGACCGG CAAGCTGGAC GCCCGCAAGA 4321 TCCGCGAGAT CCTGATCAAG GCTAAGAAAG GCGGCAAGAT CGCCGTGTAA TAATTCTAGA 4381 GTCGGGGCGG CCGGCCGCTT CGAGCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA 4441 CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT 4501 TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC ATTCATTTTA 4561 TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTAAAG CAAGTAAAAC CTCTACAAAT 4621 GTGGTAAAAT CGATAAGGAT CCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA 4681 TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT 4741 AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC 4801 TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA 4861 AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA 4921 ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT 4981 TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTATTGA CGCCGGGCAA GAGCAACTCG 5041 GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC 5101 ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA 5161 ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT 5221 TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG 5281 CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGTAGC AATGGCAACA ACGTTGCGCA 5341 AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG 5401 AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG 5461 CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG 5521 ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG 5581 AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG 5641 ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA 5701 TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT 5761 TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC 5821 TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC 5881 CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC 5941 CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC 6001 CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT 6061 CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT 6121 GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT 6181 ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT 6241 ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG 6301 CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT 6361 GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT 6421 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGGCTCGAC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.