pLV3-EF1a-GRN-3×Myc-PGK-mCherr

pLV3-EF1a-GRN-3×Myc-PGK-mCherry-Neo plasmid

PVT94136

pLV3-EF1a-GRN-3×Myc-PGK-mCherry-Neo plasmid Information

Plasmid name: pLV3-EF1a-GRN(human)-3×Myc-PGK-mCherry-Neo
Alias: NM_002087.4;CLN11; GEP; GP88; PCDGF; PEPI; PGRN
Gene lenght: 1779bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Red
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13F-72
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-GRN-3×Myc-PGK-mCherry-Neo plasmid Description

pLV3-EF1a-GRN-3×Myc-PGK-mCherry-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-EF1a-GRN-3×Myc-PGK-mCherry-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca 1441 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg 1501 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa 1561 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa 1621 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt 1681 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag 1741 gtggagagag agacagagac agatccattc gattagtgaa cggatctcga cggtATCGGT 1801 taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca 1861 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa ttcaaaattt 1921 tatcgatact agtggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 1981 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 2041 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 2101 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 2161 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 2221 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 2281 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 2341 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 2401 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 2461 ctgtgaccgg cgcctactct agagctagcg aattcGCCAC Catgtggacc ctggtgagct 2521 gggtggcctt aacagcaggg ctggtggctg gaacgcggtg cccagatggt cagttctgcc 2581 ctgtggcctg ctgcctggac cccggaggag ccagctacag ctgctgccgt ccccttctgg 2641 acaaatggcc cacaacactg agcaggcatc tgggtggccc ctgccaggtt gatgcccact 2701 gctctgccgg ccactcctgc atctttaccg tctcagggac ttccagttgc tgccccttcc 2761 cagaggccgt ggcatgcggg gatggccatc actgctgccc acggggcttc cactgcagtg 2821 cagacgggcg atcctgcttc caaagatcag gtaacaactc cgtgggtgcc atccagtgcc 2881 ctgatagtca gttcgaatgc ccggacttct ccacgtgctg tgttatggtc gatggctcct 2941 gggggtgctg ccccatgccc caggcttcct gctgtgaaga cagggtgcac tgctgtccgc 3001 acggtgcctt ctgcgacctg gttcacaccc gctgcatcac acccacgggc acccaccccc 3061 tggcaaagaa gctccctgcc cagaggacta acagggcagt ggccttgtcc agctcggtca 3121 tgtgtccgga cgcacggtcc cggtgccctg atggttctac ctgctgtgag ctgcccagtg 3181 ggaagtatgg ctgctgccca atgcccaacg ccacctgctg ctccgatcac ctgcactgct 3241 gcccccaaga cactgtgtgt gacctgatcc agagtaagtg cctctccaag gagaacgcta 3301 ccacggacct cctcactaag ctgcctgcgc acacagtggg ggatgtgaaa tgtgacatgg 3361 aggtgagctg cccagatggc tatacctgct gccgtctaca gtcgggggcc tggggctgct 3421 gcccttttac ccaggctgtg tgctgtgagg accacataca ctgctgtccc gcggggttta 3481 cgtgtgacac gcagaagggt acctgtgaac aggggcccca ccaggtgccc tggatggaga 3541 aggccccagc tcacctcagc ctgccagacc cacaagcctt gaagagagat gtcccctgtg 3601 ataatgtcag cagctgtccc tcctccgata cctgctgcca actcacgtct ggggagtggg 3661 gctgctgtcc aatcccagag gctgtctgct gctcggacca ccagcactgc tgcccccagg 3721 gctacacgtg tgtagctgag gggcagtgtc agcgaggaag cgagatcgtg gctggactgg 3781 agaagatgcc tgcccgccgg gcttccttat cccaccccag agacatcggc tgtgaccagc 3841 acaccagctg cccggtgggg cagacctgct gcccgagcct gggtgggagc tgggcctgct 3901 gccagttgcc ccatgctgtg tgctgcgagg atcgccagca ctgctgcccg gctggctaca 3961 cctgcaacgt gaaggctcga tcctgcgaga aggaagtggt ctctgcccag cctgccacct 4021 tcctggcccg tagccctcac gtgggtgtga aggacgtgga gtgtggggaa ggacacttct 4081 gccatgataa ccagacctgc tgccgagaca accgacaggg ctgggcctgc tgtccctacc 4141 gccagggcgt ctgttgtgct gatcggcgcc actgctgtcc tgctggcttc cgctgcgcag 4201 ccaggggtac caagtgtttg cgcagggagg ccccgcgctg ggacgcccct ttgagggacc 4261 cagccttgag acagctgctg CTCGAGGAAC AAAAACTAAT ATCAGAGGAA GATTTGGAAC 4321 AGAAACTAAT CAGTGAAGAA GATTTAGAGC AAAAGTTAAT TTCCGAGGAG GACTTATAGg 4381 gatccgcggc cgctgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt 4441 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac 4501 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac 4561 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca 4621 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa 4681 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca 4741 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg 4801 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc 4861 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccagccac 4921 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa 4981 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg 5041 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc 5101 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca 5161 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg 5221 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga 5281 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt 5341 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg 5401 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc 5461 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt 5521 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga 5581 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg gatctcgcga 5641 gggcagagga agtcttctaa catgcggtga cgtggaggag aatcccggcc ctatggctga 5701 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga 5761 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg 5821 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaggacga 5881 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt 5941 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct 6001 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct 6061 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg 6121 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca 6181 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg cgcatgcccg acggcgagga 6241 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt 6301 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt 6361 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct 6421 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt 6481 cttctgagtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 6541 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 6601 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 6661 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 6721 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 6781 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 6841 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt 6901 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 6961 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 7021 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 7081 gcctggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa 7141 aagaaaaggg gggactggaa gggctaattc actcccaacg aaaataagat ctgctttttg 7201 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag 7261 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc 7321 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa 7381 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat 7441 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca 7501 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 7561 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc 7621 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 7681 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctaga 7741 cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg tgtgaaattg 7801 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg 7861 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc 7921 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 7981 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct 8041 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga 8101 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc 8161 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg 8221 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg 8281 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt 8341 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt 8401 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg 8461 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact 8521 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt 8581 cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct 8641 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac 8701 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc 8761 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg 8821 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta 8881 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca 8941 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc 9001 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc 9061 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc 9121 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat 9181 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt 9241 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc 9301 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag 9361 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt 9421 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac 9481 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg 9541 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat 9601 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc 9661 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc 9721 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa 9781 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg 9841 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg 9901 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac 9961 ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga 10021 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg 10081 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa 10141 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca 10201 cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc tgcgcaactg 10261 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg 10321 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac 10381 gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.