pLV3-CMV-TRIM35-3×FLAG-CopGFP-

pLV3-CMV-TRIM35-3×FLAG-CopGFP-Puro plasmid

PVT94714

pLV3-CMV-TRIM35-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-TRIM35(human)-3×FLAG-CopGFP-Puro
Alias: NM_171982.5;HLS5; MAIR
Gene lenght: 1479bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-TRIM35-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-TRIM35-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-TRIM35-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg agcggagtcc cgacgtgtcc 2341 cccgggcctt cccgctcctt caaggaggag ttgctctgcg ccgtctgcta cgaccccttc 2401 cgcgacgcag tcactctgcg ctgcggccac aacttctgcc gcgggtgcgt gagccgctgc 2461 tgggaggtgc aggtgtcgcc cacctgccca gtgtgcaaag accgcgcgtc acccgccgac 2521 ctgcgcacca accacaccct caacaacctg gtggagaagc tgctgcgcga ggaggccgag 2581 ggcgcgcgct ggaccagcta ccgcttctcg cgtgtctgcc gcctgcaccg cggacagctc 2641 agcctcttct gcctcgagga caaggagctg ctgtgctgct cctgccaggc cgacccccga 2701 caccaggggc accgcgtgca gccggtgaag gacactgccc acgactttcg ggccaagtgc 2761 aggaacatgg agcatgcact gcgggagaag gccaaggcct tctgggccat gcggcgctcc 2821 tatgaggcca tcgccaagca caatcaggtg gaggctgcat ggctggaagg ccggatccgg 2881 caggagtttg ataagcttcg cgagttcttg agagtggagg agcaggccat tctggatgcc 2941 atggccgagg agacaaggca gaagcaactt ctggccgacg agaagatgaa gcagctcaca 3001 gaggagacgg aggtgctggc acatgagatc gagcggctgc agatggagat gaaggaggac 3061 gacgtttctt ttctcatgaa acacaagagc cgaaaacgcc gactcttctg caccatggag 3121 ccagagccag tccagcccgg catgcttatc gatgtctgca agtacctggg ctccctgcag 3181 taccgcgtct ggaagaagat gcttgcatct gtggaatctg tacccttcag ctttgacccc 3241 aacaccgcag ctggctggct ctccgtgtct gacgacctca ccagcgtcac caaccatggc 3301 taccgcgtgc aggtggagaa cccggaacgc ttctcctcgg cgccctgcct gctgggctcc 3361 cgtgtcttct cacagggctc gcacgcctgg gaggtggccc ttggggggct gcagagctgg 3421 agggtgggcg tggtacgtgt gcgccaggac tcgggcgctg agggccactc acacagctgc 3481 taccacgaca cacgctcggg cttctggtat gtctgccgca cgcagggcgt ggagggggac 3541 cactgcgtga cctcggaccc agccacgtcg cccctggtcc tggccatccc acgccgcctg 3601 cgtgtggagc tggagtgtga ggagggcgag ctgtctttct atgacgcgga gcgccactgc 3661 cacctgtaca ccttccacgc ccgctttggg gaggttcgcc cctacttcta cctggggggt 3721 gcacggggcg ccgggcctcc agagcctttg cgcatctgcc ccttgcacat cagtgtcaag 3781 gaagaactgg atggcggatc cGACTACAAA GACCATGACG GTGATTATAA AGATCATGAC 3841 ATCGACTACA AGGATGACGA TGACAAGtag cggccgcGaa ggatctgcga tcgctccggt 3901 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc 3961 ggcaattgaa cgggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg 4021 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc 4081 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac agctgaagct tcgaggggct 4141 cgcatctctc cttcacgcgc ccgccgccct acctgaggcc gccatccacg ccggttgagt 4201 cgcgttctgc cgcctcccgc ctgtggtgcc tcctgaactg cgtccgccgt ctaggtaagt 4261 ttaaagctca ggtcgagacc gggcctttgt ccggcgctcc cttggagcct acctagactc 4321 agccggctct ccacgctttg cctgaccctg cttgctcaac tctacgtctt tgtttcgttt 4381 tctgttctgc gccgttacag atccaagctg tgaccggcgc ctacgctaga cgccaccatg 4441 gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac cggcaccctg 4501 aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca gggccgcatg 4561 accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct gctgagccac 4621 gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga gaaccccttc 4681 ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta cgaggacggc 4741 ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat cggcgacttc 4801 aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa gatcatccgc 4861 agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt gggcagcttc 4921 gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga cagccacatg 4981 cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat gttcgccttc 5041 cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta ccagcacgcc 5101 ttcaagaccc ccatcgcctt cgccagatcc cgcgctcagt cgtccaattc tgccgtggac 5161 ggcaccgccg gacccggctc caccggatct cgcgagggca gaggaagtct tctaacatgc 5221 ggtgacgtgg aggagaatcc cggccctatg accgagtaca agcccacggt gcgcctcgcc 5281 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc 5341 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa 5401 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc 5461 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc 5521 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc 5581 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg 5641 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc 5701 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac 5761 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg 5821 tgcatgaccc gcaagcccgg tgcctgaaat caacctctgg attacaaaat ttgtgaaaga 5881 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 5941 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 6001 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 6061 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 6121 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 6181 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 6241 aaATCATCGT CCTTTCCTtg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 6301 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 6361 ccggctctgc ggcctcttcc gcgtctTcgc cttcgccctc agacgagtcg gatctccctt 6421 tggGccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag ctgtagatct 6481 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaaGata 6541 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 6601 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 6661 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 6721 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 6781 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 6841 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 6901 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctaTCCCG 6961 CCCCTAACTC CGCCCAtccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 7021 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 7081 cagaagtagt gaggaggctt ttttggaggc ctagactttt gcagagacca aattcgtaat 7141 catgtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 7201 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 7261 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 7321 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 7381 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 7441 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 7501 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 7561 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 7621 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 7681 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 7741 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 7801 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 7861 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 7921 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 7981 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 8041 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 8101 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 8161 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 8221 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 8281 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 8341 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 8401 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 8461 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 8521 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 8581 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 8641 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 8701 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 8761 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 8821 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 8881 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 8941 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 9001 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 9061 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 9121 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 9181 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 9241 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 9301 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 9361 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac 9421 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc 9481 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag 9541 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 9601 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc 9661 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc 9721 agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.