pLV2-CMV-EGFP-CA9-mCherry plas

pLV2-CMV-EGFP-CA9-mCherry plasmid

PVT92476

pLV2-CMV-EGFP-CA9-mCherry plasmid Information

Plasmid name: pLV2-CMV-EGFP-CA9(human)-mCherry
Alias: NM_001216.3;CAIX; MN
Gene lenght: 1377bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green,Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-EGFP-CA9-mCherry plasmid Description

pLV2-CMV-EGFP-CA9-mCherry is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-CMV-EGFP-CA9-mCherry plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggtg 2821 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac 2881 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag 2941 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg 3001 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac 3061 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag 3121 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac 3181 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg 3241 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc 3301 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac 3361 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg 3421 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg 3481 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtgga 3541 ggcagtggtg gaggcggtag cctcgagatg gctcccctgt gccccagccc ctggctccct 3601 ctgttgatcc cggcccctgc tccaggcctc actgtgcaac tgctgctgtc actgctgctt 3661 ctggtgcctg tccatcccca gaggttgccc cggatgcagg aggattcccc cttgggagga 3721 ggctcttctg gggaagatga cccactgggc gaggaggatc tgcccagtga agaggattca 3781 cccagagagg aggatccacc cggagaggag gatctacctg gagaggagga tctacctgga 3841 gaggaggatc tacctgaagt taagcctaaa tcagaagaag agggctccct gaagttagag 3901 gatctaccta ctgttgaggc tcctggagat cctcaagaac cccagaataa tgcccacagg 3961 gacaaagaag gggatgacca gagtcattgg cgctatggag gcgacccgcc ctggccccgg 4021 gtgtccccag cctgcgcggg ccgcttccag tccccggtgg atatccgccc ccagctcgcc 4081 gccttctgcc cggccctgcg ccccctggaa ctcctgggct tccagctccc gccgctccca 4141 gaactgcgcc tgcgcaacaa tggccacagt gtgcaactga ccctgcctcc tgggctagag 4201 atggctctgg gtcccgggcg ggagtaccgg gctctgcagc tgcatctgca ctggggggct 4261 gcaggtcgtc cgggctcgga gcacactgtg gaaggccacc gtttccctgc cgagatccac 4321 gtggttcacc tcagcaccgc ctttgccaga gttgacgagg ccttggggcg cccgggaggc 4381 ctggccgtgt tggccgcctt tctggaggag ggcccggaag aaaacagtgc ctatgagcag 4441 ttgctgtctc gcttggaaga aatcgctgag gaaggctcag agactcaggt cccaggactg 4501 gacatatctg cactcctgcc ctctgacttc agccgctact tccaatatga ggggtctctg 4561 actacaccgc cctgtgccca gggtgtcatc tggactgtgt ttaaccagac agtgatgctg 4621 agtgctaagc agctccacac cctctctgac accctgtggg gacctggtga ctctcggcta 4681 cagctgaact tccgagcgac gcagcctttg aatgggcgag tgattgaggc ctccttccct 4741 gctggagtgg acagcagtcc tcgggctgct gagccagtcc agctgaattc ctgcctggct 4801 gctggtgaca tcctagccct ggtttttggc ctcctttttg ctgtcaccag cgtcgcgttc 4861 cttgtgcaga tgagaaggca gcacagaagg ggaaccaaag ggggtgtgag ctaccgccca 4921 gcagaggtag ccgagactgg agcctagtct agagcggccg cggatcccgc ccctctccct 4981 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct 5041 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc 5101 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc 5161 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg 5221 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa 5281 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt 5341 ggatagttgt ggaaagagtc aaatggctca cctcaagcgt attcaacaag gggctgaagg 5401 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta 5461 catgtgttta gtcgaggtta aaaaacgtct aggccccccg aaccacgggg acgtggtttt 5521 cctttgaaaa acacgatgat aatatggtga gcaagggcga ggaggataac atggccatca 5581 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg 5641 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg 5701 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg 5761 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc 5821 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga 5881 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca 5941 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg 6001 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga 6061 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc 6121 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact 6181 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg 6241 agctgtacaa gtgaacgcgt ctggaacaat caacctctgg attacaaaat ttgtgaaaga 6301 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 6361 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc 6421 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 6481 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt 6541 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt 6601 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 6661 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg 6721 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 6781 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 6841 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa 6901 tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg 6961 aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg 7021 cagctgtaga tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact 7081 cccaacgaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg 7141 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct 7201 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca 7261 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt 7321 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact 7381 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga 7441 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg 7501 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc 7561 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg 7621 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg 7681 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca 7741 aagaaatgaa tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct 7801 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 7861 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 7921 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 7981 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg 8041 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg 8101 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga 8161 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg 8221 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag 8281 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc 8341 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg 8401 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt 8461 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc 8521 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc 8581 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg 8641 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca 8701 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc 8761 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat 8821 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt 8881 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt 8941 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc 9001 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc 9061 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata 9121 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg 9181 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc 9241 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct 9301 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa 9361 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt 9421 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca 9481 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac 9541 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 9601 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 9661 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 9721 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 9781 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 9841 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 9901 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 9961 cgaaaagtgc cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa 10021 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 10081 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat 10141 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa 10201 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag 10261 aaattgtatt tgttaaatat gtactacaaa cttagtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.