pCMV-FAM171A2-FLAG-Twin Strep-

pCMV-FAM171A2-FLAG-Twin Strep-Neo plasmid

PVT94123

pCMV-FAM171A2-FLAG-Twin Strep-Neo plasmid Information

Plasmid name: pCMV-FAM171A2(human)-FLAG-Twin Strep-Neo
Alias: NM_198475.3
Gene lenght: 2478bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-FAM171A2-FLAG-Twin Strep-Neo plasmid Description

pCMV-FAM171A2-FLAG-Twin Strep-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-FAM171A2-FLAG-Twin Strep-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTGAATT CGCCACCatg ccgccggcga gtggccccag cgtcctcgcg 961 cggctgttgc cgctgctggg gctgctgctc ggcagcgcct cccgggctcc cggcaagtcg 1021 ccgccggagc cccccagccc gcaggagatc ctgatcaagg tgcaggtgta tgtgagcggg 1081 gagctggtgc ccctggcccg ggcctcagtg gatgtgtttg ggaaccggac tctgctggca 1141 gctggcacca cagactcaga gggtgtggcc accctgcccc tcagttatcg cttgggcacc 1201 tgggtgctgg tcactgctgc ccgccctggc ttcctcacca actctgtgcc ctggcgtgtt 1261 gacaagctgc ccttgtatgc gtctgtcagc ctctacctgc tccctgagcg gccggccacg 1321 ctcatcctct atgaggacct ggtgcacatt ctcctaggct ctcccggtgc ccgctcccag 1381 cccttggtgc agttccagcg ccgggctgcc cgcctgcctg tcagctccac ctacagccag 1441 ctctgggcgt cacttacgcc tgccagcacc cagcaggaaa tgcgggcttt ccctgccttc 1501 ctgggcactg aggcctccag ctcaggcaat ggctcctggc tggagctgat gcccctgact 1561 gctgtgagcg tgcacctgct gacaggtaat gggacagagg tgccgctctc aggccccatt 1621 cacctgtccc tgcccgtgcc ctccgagact cgtgccctca ccgtgggcac cagcattcca 1681 gcctggagat ttgaccccaa gagtgggctg tgggtgcgca atggcactgg tgtaatccgg 1741 aaggaaggcc ggcagctcta ctggaccttc gtctcccccc agctggggta ctgggtggcc 1801 gccatggcct cccccacggc tgggctggtc accatcacgt cgggcatcca ggacatcggc 1861 acctaccaca ccatcttctt gctcaccatc ctggcagccc tggccctgct ggtgcttatc 1921 ctgctgtgtc tgctcatcta ctactgccgg aggcgctgcc tgaagccgag gcaacagcac 1981 cgcaagctgc agctctcggg gccctctgac ggtaacaaac gagaccaggc cacctcgatg 2041 tcccagctcc acctcatctg tgggggaccc ctggaacccg ccccgtcggg ggaccccgag 2101 gctccgcctc caggccccct ccactcggcc ttctccagct cccgggactt ggcctcctcc 2161 cgggatgact tcttccgcac caagccgcgc tctgccagcc gcccggccgc cgagccttcg 2221 ggtgcccgcg ggggcgagag cgccgggctc aagggcgctc gctcggccga gggccccggc 2281 gggctggagc ccggcctaga ggagcaccgg cgggggccct cgggggctgc ggccttcctg 2341 cacgagccgc cctcgccgcc gccgcccttc gaccactacc tgggccacaa gggggcggcc 2401 gagggcaaga cccccgactt cctgctgtcg cagtcggtgg accagctggc gcggccgccg 2461 tcgctgggcc aggcggggca gctcatcttc tgcggctcca tcgaccacct caaggacaac 2521 gtctaccgca acgtcatgcc caccctggtg atccccgcgc actacgtgcg cctcggcggc 2581 gaggcgggcg ccgccggcgt gggcgacgag ccggccccgc cggagggcac ggcacccggc 2641 ccggcgcgcg cttttcccca gcccgacccc cagcgcccgc agatgccggg ccactcgggc 2701 ccggggggcg agggcggcgg gggcggcggc gagggctggg gggccgggcg cgcggcgccc 2761 gtcagtggct cagtcaccat ccctgtgcta ttcaacgagt ccaccatggc gcagctcaac 2821 ggggagctgc aggccctgac cgagaagaag ctgctggaac tgggcgtgaa gccgcacccg 2881 cgcgcctggt tcgtgtccct cgacgggcgc tccaactcgc aagtgcgcca ctcttacatc 2941 gacctgcagg cgggcggcgg ggcacgcagc accgacgcca gcctggactc gggcgtagat 3001 gtccacgagg cgcggcccgc gcgccgccgg cccgcgaggg aggagcggga gcgcgccccg 3061 cctgccgcgc cgccgccgcc gcccgcgccc ccgcgcctgg cgctcagcga ggacacggag 3121 cccagcagca gcgagagccg cacgggcctc tgctctccgg aggacaactc gctgacgccg 3181 ctgctggacg aggtggcggc gcccgagggc cgggcggcca cggtaccccg ggggcggggc 3241 cgcagccgcg gggacagctc ccgcagcagc gccagcgagc tgcggcgcga ctcgctcacc 3301 agcccggagg acgagctggg ggcggaggtg ggcgacgagg cgggagacaa gaagagcccg 3361 tggcagcggc gggaggagcg gccgctgatg gtgttcaacg tcaagCTCGA GGATTACAAG 3421 GATGACGACG ATAAGGGATC Cagcgcctgg tcccaccctc agtttgagaa gggcggaggc 3481 tctggcggcg gaagcggagg atctgcttgg agccaccccc agttcgaaaa gtgaTGAGGG 3541 CCCGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT 3601 TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA 3661 TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG 3721 GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG 3781 GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC 3841 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 3901 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 3961 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 4021 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 4081 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 4141 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 4201 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 4261 GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG 4321 CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC 4381 CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG 4441 TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC 4501 CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC 4561 TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG 4621 GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA 4681 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT 4741 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC 4801 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG 4861 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG 4921 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC 4981 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC 5041 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG 5101 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC 5161 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG 5221 AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC 5281 GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG 5341 CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG 5401 TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG 5461 AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC 5521 ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT 5581 CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA 5641 CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT 5701 CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT 5761 ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA 5821 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG 5881 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG 5941 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA 6001 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC 6061 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 6121 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 6181 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 6241 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 6301 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 6361 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 6421 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 6481 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 6541 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 6601 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 6661 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 6721 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC 6781 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 6841 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 6901 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 6961 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 7021 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 7081 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT 7141 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 7201 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 7261 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 7321 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 7381 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 7441 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 7501 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 7561 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 7621 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 7681 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 7741 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 7801 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 7861 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 7921 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.