pTac-GST-PPM1G plasmid

pTac-GST-PPM1G plasmid

PVT95876

pTac-GST-PPM1G plasmid Information

Plasmid name: pTac-GST-PPM1G(human)
Alias: NM_177983.3;PP2CG; PP2CGAMMA; PPP2CG
Gene lenght: 1641bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: GST-F2
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pTac-GST-PPM1G plasmid Description

pTac-GST-PPM1G is a plasmid suitable for protein expression in Escherichia coli.

pTac-GST-PPM1G plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc 301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc 361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc 421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata 481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc 541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact 601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag 661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt 721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa 781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat 841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc 901 atcctccaaa atcggatctg gttccgcgtg gatccatggg tgcctacctc tcccagccca 961 acacggtgaa gtgctccggg gacggggtcg gcgccccgcg cctgccgctg ccctacggct 1021 tctccgccat gcaaggctgg cgcgtctcca tggaggatgc tcacaactgt attcctgagc 1081 tggacagtga gacagccatg ttttctgtct acgatggaca tggaggggag gaagttgcct 1141 tgtactgtgc caaatatctt cctgatatca tcaaagatca gaaggcctac aaggaaggca 1201 agctacagaa ggctttagaa gatgccttct tggctattga cgccaaattg accactgaag 1261 aagtcattaa agagctggca cagattgcag ggcgacccac tgaggatgaa gatgaaaaag 1321 aaaaagtagc tgatgaagat gatgtggaca atgaggaggc tgcactgctg catgaagagg 1381 ctaccatgac tattgaagag ctgctgacac gctacgggca gaactgtcac aagggccctc 1441 cccacagcaa atctggaggt gggacaggcg aggaaccagg gtcccagggc ctcaatgggg 1501 aggcaggacc tgaggactca actagggaaa ctccttcaca agaaaatggc cccacagcca 1561 aggcctacac aggcttttcc tccaactcgg aacgtgggac tgaggcaggc caagttggtg 1621 agcctggcat tcccactggt gaggctgggc cttcctgctc ttcagcctct gacaagctgc 1681 ctcgagttgc taagtccaag ttctttgagg acagtgagga tgagtcagat gaggcggagg 1741 aagaagagga agacagtgag gaatgcagcg aggaagagga tggctacagc agtgaggagg 1801 cagagaatga ggaagatgag gatgacaccg aggaggctga agaggacgat gaagaagaag 1861 aagaagagat gatggtgcca gggatggaag gcaaagagga gcctggctct gacagtggta 1921 caacagcggt ggtggccctg atacgaggga agcagttgat tgtagccaac gcaggagact 1981 ctcgctgtgt ggtatctgag gctggcaaag ctttagacat gtcctatgat cacaaaccag 2041 aggatgaagt agaactagca cgcatcaaga atgctggtgg caaggtcacc atggatgggc 2101 gagtcaacgg gggcctcaac ctctccagag ccattgggga ccacttctat aagagaaaca 2161 agaacctgcc acctgaggaa cagatgattt cagcccttcc tgacatcaag gtgctgactc 2221 tcactgacga ccatgaattc atggtcattg cctgtgatgg catctggaat gtgatgagca 2281 gccaggaagt tgtagatttc attcaatcaa agatcagcca gcgtgatgaa aatggggagc 2341 ttcggttatt gtcatccatt gtggaagagc tgctggatca gtgcctggca ccagacactt 2401 ctggggatgg tacagggtgt gacaacatga cctgcatcat catttgcttc aagccccgaa 2461 acacagcaga gctccagcca gagagtggca agcgaaaact agaggaggtg ctctctactg 2521 agggggctga agaaaatggc aacagcgaca agaagaagaa ggccaagcga gactagctcg 2581 agcggccgca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat gacggtgaaa 2641 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 2701 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga 2761 cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc ctcgtgatac 2821 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt 2881 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 2941 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta 3001 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 3061 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 3121 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 3181 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 3241 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 3301 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 3361 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 3421 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 3481 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 3541 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 3601 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 3661 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 3721 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 3781 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 3841 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 3901 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 3961 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 4021 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 4081 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 4141 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 4201 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 4261 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 4321 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 4381 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 4441 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 4501 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 4561 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 4621 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 4681 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 4741 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 4801 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataaatt 4861 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga 4921 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 4981 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 5041 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 5101 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 5161 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 5221 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 5281 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 5341 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 5401 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 5461 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 5521 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 5581 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 5641 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 5701 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg 5761 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 5821 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 5881 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 5941 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 6001 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag 6061 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 6121 taacaatttc acacaggaaa cagctatgac catgattacg gattcactgg ccgtcgtttt 6181 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc 6241 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt 6301 gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga 6361 aagctggctg gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca 6421 gatgcacggt tacgatgcgc ccatctacac caacgtaacc tatcccatta cggtcaatcc 6481 gccgtttgtt cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga 6541 aagctggcta caggaaggcc agacgcgaat tatttttgat ggcgttggaa tt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.