pLV3-CMV-Lmna-3×FLAG-CopGFP-Pu

pLV3-CMV-Lmna-3×FLAG-CopGFP-Puro plasmid

PVT92728

pLV3-CMV-Lmna-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Lmna(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_001002011.3;Dhe;
Gene lenght: 1995bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Lmna-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Lmna-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Lmna-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg agaccccgtc acagcggcgc 2341 gccacccgca gtggggcgca ggccagctct accccactgt cgcccactcg gatcacccgg 2401 ctgcaggaga aggaggacct gcaggagctc aatgaccgcc tggccgtgta catcgatcgc 2461 gtgcgttccc tggagaccga gaacgcgggg ctgcgccttc gcatcactga gtctgaagag 2521 gtggtcagcc gagaggtgtc cggcatcaag gcggcctacg aggccgagct gggggatgcc 2581 cgcaagaccc ttgattctgt ggccaaggag cgcgcccgcc tccagctaga gctgagcaaa 2641 gtgcgtgagg agttcaagga gctgaaggct cgcaacacca agaaggaggg ggacttgttg 2701 gctgcgcagg cccggctcaa ggacctcgag gctcttctca actccaagga agctgccctg 2761 agcactgctc tcagtgagaa gcgcacattg gagggcgagc tccatgacct gcgggggcag 2821 gtagccaagc ttgaggcggc cctgggagag gctaagaagc agcttcagga tgagatgctg 2881 aggcgagtgg atgctgagaa caggctacag acgctgaagg aggagcttga cttccagaag 2941 aacatttaca gcgaggaact gcgtgagacc aagcgccggc atgagacgcg gcttgtggag 3001 atcgataacg ggaagcagcg agagtttgag agccggctgg cagatgccct gcaggagctg 3061 cgggctcagc atgaggacca ggtggaacag tataagaagg agctagaaaa gacatactcc 3121 gccaagctgg ataatgccag gcagtctgct gagaggaaca gcaacctcgt gggggctgcc 3181 catgaggaac tgcagcagtc tcgaatccgc attgacagcc tctcggccca gctcagccag 3241 ctccaaaagc agttggcagc caaggaggca aagctgcgtg acctggagga ctcgctggcc 3301 cgtgagcgcg ataccagccg gcgcctgctg gctgagaaag agcgagagat ggcggagatg 3361 cgggcgagga tgcagcagca gctggacgag taccaggagc tgctggacat caagctggcc 3421 ctggacatgg agatccatgc ctatcgaaag ctgctggagg gcgaggagga gaggctgcgc 3481 ctgtccccca gccctacctc gcagcgcagc cgtggccgcg cctcctccca ctcatcccag 3541 tctcagggtg gaggcagcgt caccaaaaag cgcaagctgg agtcttccga gagccggagc 3601 agcttctcgc agcatgctcg cactagcggg cgtgtggcgg tagaggaagt cgatgaagag 3661 ggaaagttcg tgcggctgcg caacaagtcc aacgaggacc agtccatggg caactggcag 3721 atcaggcgtc agaatggtga cgatcctttg atgacctatc gcttcccacc gaagttcacc 3781 ctaaaggctg ggcaggtggt gacgatctgg gcttcaggag ctggggccac ccatagcccc 3841 cctactgact tggtgtggaa ggcgcagaac acctggggct gtgggagcag ccttcgcacc 3901 gctctcatca actccactgg agaagaagtg gccatgcgca agctggtgcg ctcactgacc 3961 atggttgagg acaatgagga tgacgacgag gatggagaag agctcctcca tcaccaccgt 4021 ggttcccact gcagcggctc gggggacccc gctgagtaca acctgcgctc acgcaccgtg 4081 ctgtgcggga cgtgtgggca gcctgctgac aaggctgccg gtggagcggg agcccaggtg 4141 ggcggatcca tctcctctgg ctcttctgcc tccagtgtca cagtcactcg aagcttccgc 4201 agtgtggggg gcagtggggg tggcagcttc ggggacaacc tagtcacccg ctcctacctc 4261 ctgggcaact ccagtccccg gagccagagc tcccagaact gcagcatcat gggatccGAC 4321 TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG ACTACAAGGA TGACGATGAC 4381 AAGtagcggc cgcGaaggat ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca 4441 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaacggg tgcctagaga 4501 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag 4561 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg 4621 tttgccgcca gaacacagct gaagcttcga ggggctcgca tctctccttc acgcgcccgc 4681 cgccctacct gaggccgcca tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt 4741 ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa agctcaggtc gagaccgggc 4801 ctttgtccgg cgctcccttg gagcctacct agactcagcc ggctctccac gctttgcctg 4861 accctgcttg ctcaactcta cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc 4921 aagctgtgac cggcgcctac gctagacgcc accatggaga gcgacgagag cggcctgccc 4981 gccatggaga tcgagtgccg catcaccggc accctgaacg gcgtggagtt cgagctggtg 5041 ggcggcggag agggcacccc caagcagggc cgcatgacca acaagatgaa gagcaccaaa 5101 ggcgccctga ccttcagccc ctacctgctg agccacgtga tgggctacgg cttctaccac 5161 ttcggcacct accccagcgg ctacgagaac cccttcctgc acgccatcaa caacggcggc 5221 tacaccaaca cccgcatcga gaagtacgag gacggcggcg tgctgcacgt gagcttcagc 5281 taccgctacg aggccggccg cgtgatcggc gacttcaagg tggtgggcac cggcttcccc 5341 gaggacagcg tgatcttcac cgacaagatc atccgcagca acgccaccgt ggagcacctg 5401 caccccatgg gcgataacgt gctggtgggc agcttcgccc gcaccttcag cctgcgcgac 5461 ggcggctact acagcttcgt ggtggacagc cacatgcact tcaagagcgc catccacccc 5521 agcatcctgc agaacggggg ccccatgttc gccttccgcc gcgtggagga gctgcacagc 5581 aacaccgagc tgggcatcgt ggagtaccag cacgccttca agacccccat cgccttcgcc 5641 agatcccgcg ctcagtcgtc caattctgcc gtggacggca ccgccggacc cggctccacc 5701 ggatctcgcg agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc 5761 cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 5821 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 5881 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 5941 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 6001 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 6061 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 6121 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 6181 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 6241 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 6301 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 6361 tgaaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt 6421 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc 6481 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag 6541 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc 6601 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc 6661 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg 6721 ctgttgggca ctgacaattc cgtggtgttg tcggggaaAT CATCGTCCTT TCCTtggctg 6781 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc 6841 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt 6901 ctTcgccttc gccctcagac gagtcggatc tccctttggG ccgcctcccc gcctggtacc 6961 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg 7021 gggactggaa gggctaattc actcccaacg aaGataagat ctgctttttg cttgtactgg 7081 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact 7141 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg 7201 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag 7261 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga 7321 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 7381 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 7441 caatgtatct tatcatgtct ggctctagct aTCCCGCCCC TAACTCCGCC CAtcccgccc 7501 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat 7561 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt 7621 ggaggcctag acttttgcag agaccaaatt cgtaatcatg tcatagctgt ttcctgtgtg 7681 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 7741 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 7801 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 7861 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 7921 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 7981 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 8041 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 8101 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 8161 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 8221 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 8281 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 8341 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 8401 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 8461 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 8521 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 8581 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 8641 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 8701 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 8761 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 8821 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 8881 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 8941 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 9001 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 9061 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 9121 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 9181 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 9241 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 9301 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 9361 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 9421 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 9481 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 9541 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 9601 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 9661 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 9721 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 9781 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 9841 attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga 9901 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 9961 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg 10021 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat 10081 accgcacaga tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg 10141 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 10201 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 10261 taaaacgacg gccagtgcca agctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.