pCMV-RNF31-3×Myc-Neo plasmid

pCMV-RNF31-3×Myc-Neo plasmid

PVT96232

pCMV-RNF31-3×Myc-Neo plasmid Information

Plasmid name: pCMV-RNF31(human)-3×Myc-Neo
Alias: NM_017999.5;HOIP; IMD115; Paul; ZIBRA
Gene lenght: 3216bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-RNF31-3×Myc-Neo plasmid Description

pCMV-RNF31-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-RNF31-3×Myc-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 CACTAGTCCA GTGTGGTGGA ATTCgccacc atgccggggg aggaagagga gcgggccttc 961 ctggtggccc gcgaggagct ggcgagcgcc ctgaggaggg attccgggca ggcgttttcc 1021 ctggagcagc tccggccgct actagccagc tctctgccgc tagccgcccg ctacctgcag 1081 ctggacgccg cacgccttgt ccgctgcaac gctcatgggg agccccgaaa ctacctcaac 1141 accctgtcca cggctctgaa catcctggag aaatacggcc gcaaccttct cagccctcag 1201 cggcctcggt actggcgtgg tgtcaagttt aataaccctg tctttcgcag cacggtggat 1261 gctgtgcagg ggggccgaga tgtgctgcga ttatatggct acacagagga gcaaccagat 1321 gggttgagct tccccgaagg gcaggaggag ccagatgagc accaggttgc tacagtcaca 1381 ctggaagtac tgctgcttcg gacagagctc agcctgctat tgcagaatac tcatccaaga 1441 cagcaggcac tggagcagct gttggaagac aaggttgaag atgatatgct gcagctttca 1501 gaatttgacc ccctattgag agagattgct cctggccccc tcaccacacc ctctgtccca 1561 ggctccactc ctggtccctg cttcctctgt ggttctgccc caggcacact gcactgccca 1621 tcctgtaaac aggccctgtg tccagcctgt gaccacctgt tccatggaca cccatcccgt 1681 gctcatcacc tccgccagac cctgcctggg gtcctgcagg gtacccacct gagccccagt 1741 ttacctgcct cagcccaacc acggccccag tcgacctccc tgctggccct gggagacagc 1801 tctctttctt cccctaatcc tgcaagtgct catttgccct ggcactgtgc tgcctgtgcc 1861 atgctaaatg agccttgggc agtgctctgt gtggcctgtg atcggccccg aggctgtaag 1921 gggttggggt tgggaactga gggtccccaa ggaactggag gcctagaacc tgatcttgca 1981 cggggtcggt gggcctgcca gagctgtacc tttgagaatg aggcagctgc tgtgctatgt 2041 tccatatgtg agcgacctcg gctggcccag cctcccagct tggtggtgga ttcccgagat 2101 gctggcattt gcctgcaacc ccttcagcag ggggatgctt tgctggcctc tgcccagagt 2161 caagtctggt actgtattca ctgtaccttc tgcaactcga gccctggctg ggtgtgtgtt 2221 atgtgcaacc ggactagtag ccccattcca gcacaacatg ccccccggcc ctatgccagc 2281 tctttggaaa agggaccccc caagcctggg cccccacgac gccttagtgc ccccctgccc 2341 agttcctgtg gagatcctga gaagcagcgc caagacaaga tgcgggaaga aggcctccag 2401 ctagtgagca tgatccggga aggggaagcc gcaggtgcct gtccagagga gatcttctcg 2461 gctctgcagt actcgggcac tgaggtgcct ctgcagtggt tgcgctcaga actgccctac 2521 gtcctggaga tggtggctga gctggctgga cagcaggacc ctgggctggg tgccttttcc 2581 tgtcaggagg cccggagagc ctggctggat cgtcatggca accttgatga agctgtggag 2641 gagtgtgtga ggaccaggcg aaggaaggtg caggagctcc agtctctagg ctttgggcct 2701 gaggaggggt ctctccaggc attgttccag cacggaggtg atgtgtcacg ggccctgact 2761 gagctacagc gccaacgcct agagcccttc cgccagcgcc tctgggacag tggccctgag 2821 cccacccctt cctgggatgg gccagacaag cagagcctgg tcaggcggct tttggcagtc 2881 tacgcactcc ccagctgggg ccgggcagag ctggcactgt cactgctgca ggagacaccc 2941 aggaactatg agttggggga tgtggtagaa gctgtgaggc acagccagga ccgggccttc 3001 ctgcgccgct tgcttgccca ggagtgtgcc gtgtgtggct gggccctgcc ccacaaccgg 3061 atgcaggccc tgacttcctg tgagtgcacc atctgtcctg actgcttccg ccagcacttc 3121 accatcgcct tgaaggagaa gcacatcaca gacatggtgt gccctgcctg tggccgcccc 3181 gacctcaccg atgacacaca gttgctcagc tacttctcta cccttgacat ccagcttcgc 3241 gagagcctag agccagatgc ctatgcgttg ttccataaga agctgaccga gggtgtgctg 3301 atgcgggacc ccaagttctt gtggtgtgcc cagtgctcct ttggcttcat atatgagcgt 3361 gagcagctgg aggcaacttg tccccagtgt caccagacct tctgtgtgcg ctgcaagcgc 3421 cagtgggagg agcagcaccg aggtcggagc tgtgaggact tccagaactg gaaacgcatg 3481 aacgacccag aataccaggc ccagggccta gcaatgtatc ttcaggaaaa cggcattgac 3541 tgccccaaat gcaagttctc gtacgccctg gcccgaggag gctgcatgca ctttcactgt 3601 acccagtgcc gccaccagtt ctgcagcggc tgctacaatg ccttttacgc caagaataaa 3661 tgtccagagc ctaactgcag ggtgaaaaag tccctgcacg gccaccaccc tcgagactgc 3721 ctcttctacc tgcgggactg gactgctctc cggcttcaga agctgctaca ggacaataac 3781 gtcatgttta atacagagcc tccagctggg gcccgggcag tccctggagg cggctgccga 3841 gtgatagagc agaaggaggt tcccaatggg ctcagggacg aagcttgtgg caaggaaact 3901 ccagctggct atgccggcct gtgccaggca cactacaaag agtatcttgt gagcctcatc 3961 aatgcccact cgctggaccc agccaccttg tatgaggtgg aagagctgga gacggccact 4021 gagcgctacc tgcacgtacg cccccagcct ttggctggag aggatccccc tgcttaccag 4081 gcccgcttgt tacagaagct gacagaagag gtacccttgg gacagagtat cccccgcagg 4141 cggaagCTCG AGGAACAAAA ACTAATATCA GAGGAAGATT TGGAACAGAA ACTAATCAGT 4201 GAAGAAGATT TAGAGCAAAA GTTAATTTCC GAGGAGGACT TAtgaGGGCC CGTTTAAACC 4261 CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT CTGTTGTTTG CCCCTCCCCC 4321 GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC TTTCCTAATA AAATGAGGAA 4381 ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG GGGGTGGGGT GGGGCAGGAC 4441 AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG GGGATGCGGT GGGCTCTATG 4501 GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT ATCCCCACGC GCCCTGTAGC 4561 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 4621 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 4681 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 4741 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 4801 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 4861 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 4921 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTAATTC 4981 TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA 5041 TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG AAAGTCCCCA GGCTCCCCAG 5101 CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA 5161 CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC 5221 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC CTCTGAGCTA TTCCAGAAGT 5281 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT 5341 CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT TCGCATGATT GAACAAGATG 5401 GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC 5461 AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG 5521 TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA ACTGCAGGAC GAGGCAGCGC 5581 GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG 5641 AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC 5701 ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC 5761 TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA 5821 CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG 5881 CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC CGACGGCGAG GATCTCGTCG 5941 TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT 6001 TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC 6061 GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA 6121 TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG 6181 CGGGACTCTG GGGTTCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT CACGAGATTT 6241 CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC GGGACGCCGG 6301 CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC CCAACTTGTT 6361 TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA CAAATAAAGC 6421 ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT CTTATCATGT 6481 CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC TGTTTCCTGT 6541 GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA TAAAGTGTAA 6601 AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT CACTGCCCGC 6661 TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG 6721 AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT 6781 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 6841 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 6901 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 6961 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 7021 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 7081 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 7141 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 7201 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 7261 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 7321 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 7381 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 7441 ACAAACCACC GCTGGTAGCG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 7501 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 7561 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 7621 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG 7681 TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT 7741 AGTTGCCTGA CTCCCCGTCG TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC 7801 CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA 7861 CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA 7921 GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA 7981 CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG TCGTTTGGTA TGGCTTCATT 8041 CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC CCCATGTTGT GCAAAAAAGC 8101 GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT 8161 CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC 8221 TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC GACCGAGTTG 8281 CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT AGCAGAACTT TAAAAGTGCT 8341 CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG ATCTTACCGC TGTTGAGATC 8401 CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG 8461 CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC 8521 ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG 8581 TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT 8641 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGTCGAC GGATCGGGAG ATCTCCCGAT 8701 CCCCTATGGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.