pCMV-EGFP-MSH3-Neo plasmid

pCMV-EGFP-MSH3-Neo plasmid

PVT92664

pCMV-EGFP-MSH3-Neo plasmid Information

Plasmid name: pCMV-EGFP-MSH3(human)-Neo
Alias: NM_002439.5;DUP; FAP4; MRP1;
Gene lenght: 3411bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-MSH3-Neo plasmid Description

pCMV-EGFP-MSH3-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-MSH3-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCgcc accatggtga gcaagggcga ggagctgttc 901 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 961 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 1021 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 1081 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 1141 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 1201 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 1261 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 1321 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 1381 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 1441 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 1501 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 1561 atcactctcg gcatggacga gctgtacaag gGaGGCGGAG GTAGTGGAGG AGGGGGAAGC 1621 ggaggcggag ggagtGAATT Catgtctcgc cggaagcctg cgtcgggcgg cctcgctgcc 1681 tccagctcag cccctgcgag gcaagcggtt ttgagccgat tcttccagtc tacgggaagc 1741 ctgaaatcca cctcctcctc cacaggtgca gccgaccagg tggaccctgg cgctgcagcg 1801 gctgcagcgg ccgcagcggc cgcagcgccc ccagcgcccc cagctcccgc cttcccgccc 1861 cagctgccgc cgcacatagc tacagaaatt gacagaagaa agaagagacc attggaaaat 1921 gatgggcctg ttaaaaagaa agtaaagaaa gtccaacaaa aggaaggagg aagtgatctg 1981 ggaatgtctg gcaactctga gccaaagaaa tgtctgagga ccaggaatgt ttcaaagtct 2041 ctggaaaaat tgaaagaatt ctgctgcgat tctgcccttc ctcaaagtag agtccagaca 2101 gaatctctgc aggagagatt tgcagttctg ccaaaatgta ctgattttga tgatatcagt 2161 cttctacacg caaagaatgc agtttcttct gaagattcga aacgtcaaat taatcaaaag 2221 gacacaacac tttttgatct cagtcagttt ggatcatcaa atacaagtca tgaaaattta 2281 cagaaaactg cttccaaatc agctaacaaa cggtccaaaa gcatctatac gccgctagaa 2341 ttacaataca tagaaatgaa gcagcagcac aaagatgcag ttttgtgtgt ggaatgtgga 2401 tataagtata gattctttgg ggaagatgca gagattgcag cccgagagct caatatttat 2461 tgccatttag atcacaactt tatgacagca agtataccta ctcacagact gtttgttcat 2521 gtacgccgcc tggtggcaaa aggatataag gtgggagttg tgaagcaaac tgaaactgca 2581 gcattaaagg ccattggaga caacagaagt tcactctttt cccggaaatt gactgccctt 2641 tatacaaaat ctacacttat tggagaagat gtgaatcccc taatcaagct ggatgatgct 2701 gtaaatgttg atgagataat gactgatact tctaccagct atcttctgtg catctctgaa 2761 aataaggaaa atgttaggga caaaaaaaag ggcaacattt ttattggcat tgtgggagtg 2821 cagcctgcca caggcgaggt tgtgtttgat agtttccagg actctgcttc tcgttcagag 2881 ctagaaaccc ggatgtcaag cctgcagcca gtagagctgc tgcttccttc ggccttgtcc 2941 gagcaaacag aggcgctcat ccacagagcc acatctgtta gtgtgcagga tgacagaatt 3001 cgagtcgaaa ggatggataa catttatttt gaatacagcc atgctttcca ggcagttaca 3061 gagttttatg caaaagatac agttgacatc aaaggttctc aaattatttc tggcattgtt 3121 aacttagaga agcctgtgat ttgctctttg gctgccatca taaaatacct caaagaattc 3181 aacttggaaa agatgctctc caaacctgag aattttaaac agctatcaag taaaatggaa 3241 tttatgacaa ttaatggaac aacattaagg aatctggaaa tcctacagaa tcagactgat 3301 atgaaaacca aaggaagttt gctgtgggtt ttagaccaca ctaaaacttc atttgggaga 3361 cggaagttaa agaagtgggt gacccagcca ctccttaaat taagggaaat aaatgcccgg 3421 cttgatgctg tatcggaagt tctccattca gaatctagtg tgtttggtca gatagaaaat 3481 catctacgta aattgcccga catagagagg ggactctgta gcatttatca caaaaaatgt 3541 tctacccaag agttcttctt gattgtcaaa actttatatc acctaaagtc agaatttcaa 3601 gcaataatac ctgctgttaa ttcccacatt cagtcagact tgctccggac cgttatttta 3661 gaaattcctg aactcctcag tccagtggag cattacttaa agatactcaa tgaacaagct 3721 gccaaagttg gggataaaac tgaattattt aaagaccttt ctgacttccc tttaataaaa 3781 aagaggaagg atgaaattca aggtgttatt gacgagatcc gaatgcattt gcaagaaata 3841 cgaaaaatac taaaaaatcc ttctgcacaa tatgtgacag tatcaggaca ggagtttatg 3901 atagaaataa agaactctgc tgtatcttgt ataccaactg attgggtaaa ggttggaagc 3961 acaaaagctg tgagccgctt tcactctcct tttattgtag aaaattacag acatctgaat 4021 cagctccggg agcagctagt ccttgactgc agtgctgaat ggcttgattt tctagagaaa 4081 ttcagtgaac attatcactc cttgtgtaaa gcagtgcatc acctagcaac tgttgactgc 4141 attttctccc tggccaaggt cgctaagcaa ggagattact gcagaccaac tgtacaagaa 4201 gaaagaaaaa ttgtaataaa aaatggaagg caccctgtga ttgatgtgtt gctgggagaa 4261 caggatcaat atgtcccaaa taatacagat ttatcagagg actcagagag agtaatgata 4321 attaccggac caaacatggg tggaaagagc tcctacataa aacaagttgc attgattacc 4381 atcatggctc agattggctc ctatgttcct gcagaagaag cgacaattgg gattgtggat 4441 ggcattttca caaggatggg tgctgcagac aatatatata aaggacagag tacatttatg 4501 gaagaactga ctgacacagc agaaataatc agaaaagcaa catcacagtc cttggttatc 4561 ttggatgaac taggaagagg gacgagcact catgatggaa ttgccattgc ctatgctaca 4621 cttgagtatt tcatcagaga tgtgaaatcc ttaaccctgt ttgtcaccca ttatccgcca 4681 gtttgtgaac tagaaaaaaa ttactcacac caggtgggga attaccacat gggattcttg 4741 gtcagtgagg atgaaagcaa actggatcca ggcgcagcag aacaagtccc tgattttgtc 4801 accttccttt accaaataac tagaggaatt gcagcaagga gttatggatt aaatgtggct 4861 aaactagcag atgttcctgg agaaattttg aagaaagcag ctcacaagtc aaaagagctg 4921 gaaggattaa taaatacgaa aagaaagaga ctcaagtatt ttgcaaagtt atggacgatg 4981 cataatgcac aagacctgca gaagtggaca gaggagttca acatggaaga aacacagact 5041 tctcttcttc attaaGGATC CtGATATCCA GCACAGTGGC GGCCGCTCGA GTCTAGAGGG 5101 CCCGTTTAAA CCCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT 5161 TGCCCCTCCC CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA 5221 TAAAATGAGG AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG 5281 GTGGGGCAGG ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG 5341 GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC 5401 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 5461 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 5521 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 5581 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 5641 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 5701 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 5761 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 5821 GCGAATTAAT TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG 5881 CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC 5941 CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG 6001 TCCCGCCCCT AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC 6061 CCCATGGCTG ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC 6121 TATTCCAGAA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG 6181 GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA 6241 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT 6301 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC 6361 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG 6421 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG 6481 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC 6541 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC 6601 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG 6661 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC 6721 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG 6781 AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC 6841 GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG 6901 CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG 6961 TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG 7021 AGTTCTTCTG AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC 7081 ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT 7141 CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA 7201 CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT 7261 CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT 7321 ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA 7381 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG 7441 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG 7501 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA 7561 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC 7621 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 7681 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 7741 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 7801 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 7861 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 7921 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 7981 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 8041 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 8101 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 8161 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 8221 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 8281 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTTTTTTT GTTTGCAAGC AGCAGATTAC 8341 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 8401 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 8461 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC 8521 TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT 8581 TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT 8641 ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT 8701 ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC 8761 CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA 8821 TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG 8881 TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT 8941 GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC 9001 AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT 9061 AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG 9121 GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC 9181 TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC 9241 GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT 9301 TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG 9361 AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG 9421 CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA 9481 ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTCG ACGGATCGGG 9541 AGATCTCCCG ATCCCCTATG GT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.