pCMV-MSH3-3×FLAG-Neo plasmid

pCMV-MSH3-3×FLAG-Neo plasmid

PVT94182

pCMV-MSH3-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-MSH3(human)-3×FLAG-Neo
Alias: NM_002439.5;DUP; FAP4; MRP1;
Gene lenght: 3411bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-MSH3-3×FLAG-Neo plasmid Description

pCMV-MSH3-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-MSH3-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgtc tcgccggaag cctgcgtcgg 781 gcggcctcgc tgcctccagc tcagcccctg cgaggcaagc ggttttgagc cgattcttcc 841 agtctacggg aagcctgaaa tccacctcct cctccacagg tgcagccgac caggtggacc 901 ctggcgctgc agcggctgca gcggccgcag cggccgcagc gcccccagcg cccccagctc 961 ccgccttccc gccccagctg ccgccgcaca tagctacaga aattgacaga agaaagaaga 1021 gaccattgga aaatgatggg cctgttaaaa agaaagtaaa gaaagtccaa caaaaggaag 1081 gaggaagtga tctgggaatg tctggcaact ctgagccaaa gaaatgtctg aggaccagga 1141 atgtttcaaa gtctctggaa aaattgaaag aattctgctg cgattctgcc cttcctcaaa 1201 gtagagtcca gacagaatct ctgcaggaga gatttgcagt tctgccaaaa tgtactgatt 1261 ttgatgatat cagtcttcta cacgcaaaga atgcagtttc ttctgaagat tcgaaacgtc 1321 aaattaatca aaaggacaca acactttttg atctcagtca gtttggatca tcaaatacaa 1381 gtcatgaaaa tttacagaaa actgcttcca aatcagctaa caaacggtcc aaaagcatct 1441 atacgccgct agaattacaa tacatagaaa tgaagcagca gcacaaagat gcagttttgt 1501 gtgtggaatg tggatataag tatagattct ttggggaaga tgcagagatt gcagcccgag 1561 agctcaatat ttattgccat ttagatcaca actttatgac agcaagtata cctactcaca 1621 gactgtttgt tcatgtacgc cgcctggtgg caaaaggata taaggtggga gttgtgaagc 1681 aaactgaaac tgcagcatta aaggccattg gagacaacag aagttcactc ttttcccgga 1741 aattgactgc cctttataca aaatctacac ttattggaga agatgtgaat cccctaatca 1801 agctggatga tgctgtaaat gttgatgaga taatgactga tacttctacc agctatcttc 1861 tgtgcatctc tgaaaataag gaaaatgtta gggacaaaaa aaagggcaac atttttattg 1921 gcattgtggg agtgcagcct gccacaggcg aggttgtgtt tgatagtttc caggactctg 1981 cttctcgttc agagctagaa acccggatgt caagcctgca gccagtagag ctgctgcttc 2041 cttcggcctt gtccgagcaa acagaggcgc tcatccacag agccacatct gttagtgtgc 2101 aggatgacag aattcgagtc gaaaggatgg ataacattta ttttgaatac agccatgctt 2161 tccaggcagt tacagagttt tatgcaaaag atacagttga catcaaaggt tctcaaatta 2221 tttctggcat tgttaactta gagaagcctg tgatttgctc tttggctgcc atcataaaat 2281 acctcaaaga attcaacttg gaaaagatgc tctccaaacc tgagaatttt aaacagctat 2341 caagtaaaat ggaatttatg acaattaatg gaacaacatt aaggaatctg gaaatcctac 2401 agaatcagac tgatatgaaa accaaaggaa gtttgctgtg ggttttagac cacactaaaa 2461 cttcatttgg gagacggaag ttaaagaagt gggtgaccca gccactcctt aaattaaggg 2521 aaataaatgc ccggcttgat gctgtatcgg aagttctcca ttcagaatct agtgtgtttg 2581 gtcagataga aaatcatcta cgtaaattgc ccgacataga gaggggactc tgtagcattt 2641 atcacaaaaa atgttctacc caagagttct tcttgattgt caaaacttta tatcacctaa 2701 agtcagaatt tcaagcaata atacctgctg ttaattccca cattcagtca gacttgctcc 2761 ggaccgttat tttagaaatt cctgaactcc tcagtccagt ggagcattac ttaaagatac 2821 tcaatgaaca agctgccaaa gttggggata aaactgaatt atttaaagac ctttctgact 2881 tccctttaat aaaaaagagg aaggatgaaa ttcaaggtgt tattgacgag atccgaatgc 2941 atttgcaaga aatacgaaaa atactaaaaa atccttctgc acaatatgtg acagtatcag 3001 gacaggagtt tatgatagaa ataaagaact ctgctgtatc ttgtatacca actgattggg 3061 taaaggttgg aagcacaaaa gctgtgagcc gctttcactc tccttttatt gtagaaaatt 3121 acagacatct gaatcagctc cgggagcagc tagtccttga ctgcagtgct gaatggcttg 3181 attttctaga gaaattcagt gaacattatc actccttgtg taaagcagtg catcacctag 3241 caactgttga ctgcattttc tccctggcca aggtcgctaa gcaaggagat tactgcagac 3301 caactgtaca agaagaaaga aaaattgtaa taaaaaatgg aaggcaccct gtgattgatg 3361 tgttgctggg agaacaggat caatatgtcc caaataatac agatttatca gaggactcag 3421 agagagtaat gataattacc ggaccaaaca tgggtggaaa gagctcctac ataaaacaag 3481 ttgcattgat taccatcatg gctcagattg gctcctatgt tcctgcagaa gaagcgacaa 3541 ttgggattgt ggatggcatt ttcacaagga tgggtgctgc agacaatata tataaaggac 3601 agagtacatt tatggaagaa ctgactgaca cagcagaaat aatcagaaaa gcaacatcac 3661 agtccttggt tatcttggat gaactaggaa gagggacgag cactcatgat ggaattgcca 3721 ttgcctatgc tacacttgag tatttcatca gagatgtgaa atccttaacc ctgtttgtca 3781 cccattatcc gccagtttgt gaactagaaa aaaattactc acaccaggtg gggaattacc 3841 acatgggatt cttggtcagt gaggatgaaa gcaaactgga tccaggcgca gcagaacaag 3901 tccctgattt tgtcaccttc ctttaccaaa taactagagg aattgcagca aggagttatg 3961 gattaaatgt ggctaaacta gcagatgttc ctggagaaat tttgaagaaa gcagctcaca 4021 agtcaaaaga gctggaagga ttaataaata cgaaaagaaa gagactcaag tattttgcaa 4081 agttatggac gatgcataat gcacaagacc tgcagaagtg gacagaggag ttcaacatgg 4141 aagaaacaca gacttctctt cttcatGGAT CCGACTACAA AGACCATGAC GGTGATTATA 4201 AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGTA GTGAGGGCCC GATATCTCGA 4261 CAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC 4321 TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG 4381 TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT 4441 GTGGCCCGTT GTCAGGCAAC GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC 4501 TGGTTGGGGC ATTGCCACCA CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC 4561 TATTGCCACG GCGGAACTCA TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT 4621 GTTGGGCACT GACAATTCCG TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT 4681 CGCCTGTGTT GCCACCTGGA TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT 4741 CAATCCAGCG GACCTTCCTT CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT 4801 TCGCCTTCGC CCTCAGACGA GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGAAACGG 4861 GGGAGGCTAA CTGAAACACG GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA 4921 ATAAAAAGAC AGAATAAAAC GCACGGGTGT TGGGTCGTTT GTTCATAAAC GCGGGGTTCG 4981 GTCCCAGGGC TGGCACTCTG TCGATACCCC ACCGAGACCC CATTGGGGCC AATACGCCCG 5041 CGTTTCTTCC TTTTCCCCAC CCCACCCCCC AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC 5101 CAACGTCGGG GCGGCAGGCC CTGCCATAGC AGATCTGCGC AGCTGGGGCT CTAGGGGGTA 5161 TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT 5221 GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT 5281 CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGCATCCCTT TAGGGTTCCG 5341 ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG 5401 TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA 5461 TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA 5521 TTTATAAGGG ATTTTGGGGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA 5581 ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC 5641 TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA 5701 AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA 5761 ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT 5821 TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC 5881 TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG 5941 CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT 6001 CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA 6061 TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG 6121 TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA 6181 CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT 6241 GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG 6301 CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA 6361 ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT 6421 CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC 6481 GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC 6541 GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA 6601 AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG 6661 GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC 6721 TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT 6781 CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGCGAA ATGACCGACC AAGCGACGCC 6841 CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG 6901 AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT 6961 CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT 7021 CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT 7081 CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC 7141 ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG 7201 AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT 7261 TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG 7321 AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT 7381 CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 7441 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 7501 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 7561 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 7621 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 7681 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 7741 ATGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 7801 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 7861 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 7921 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 7981 TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 8041 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 8101 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 8161 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 8221 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 8281 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC 8341 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT 8401 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC 8461 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC 8521 TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG 8581 TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG 8641 CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG 8701 ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG 8761 TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT 8821 CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA 8881 ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC 8941 ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC 9001 AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC 9061 TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC 9121 CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA 9181 ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT 9241 TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT 9301 CGACGGATCG GGAGATCTCC CGATCCCCTA TGGTCGACTC TCAGTACAAT CTGCTCTGAT 9361 GCCGCATAGT TAAGCCAGTA TCTGCTCCCT GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC 9421 GCGAGCAAAA TTTAAGCTAC AACAAGGCAA GGCTTGACCG ACAATTGCAT GAAGAATCTG 9481 CTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.