pAAV-U6-Ocm-shRNA3-ZsGreen1 pl

pAAV-U6-Ocm-shRNA3-ZsGreen1 plasmid

PVT96026

pAAV-U6-Ocm-shRNA3-ZsGreen1 plasmid Information

Plasmid name: pAAV-U6-Ocm(rat)-shRNA3-ZsGreen1
Alias: GGATATCTTCCGGTTCATAGA
Gene lenght:
Host: Mammalian cells,Adeno associated virus
Use(s): Interference silence
Fragment type: shRNA
Species: Rat
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pAAV-U6-Ocm-shRNA3-ZsGreen1 plasmid Description

pAAV-U6-Ocm-shRNA3-ZsGreen1 is a plasmid suitable for interference silencing in mammalian cells, and can also be used for adeno-associated virus packaging.

pAAV-U6-Ocm-shRNA3-ZsGreen1 plasmid Sequence

1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc 61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca 121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa 181 ttacggggtc attagttcat agcccatata tggagttccg CGTTACATAA CTTACGGTAA 241 ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA ATGACGTATG 301 TTCCCATAGT AACGcCAATA GGGACTTTCC ATTGACGTCA ATGGGTGGAG TATTTACGGT 361 AAACTGCCCA CTTGGCAGTA CATCAAGTGT ATCATATGCC AAGTACGCCC CCTATTGACG 421 TCAATGACGG TAAATGGCCC GCCTGGCATT ATGCCCAGTA CATGACCTTA TGGGACTTTC 481 CTACTTGGCA GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC 541 AGTACATCAA TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA 601 TTGACGTCAA TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA 661 ACAACTCCGC CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 721 GCAGAGCTCG TTTAGTGAAC CGTCAGATCG CCTGGAGACG CCATCCACGC TGTTTTGACC 781 TCCATAGAAG ACACCGGGAC CGATCCAGCC TCCGCGGATT CGAATCCCGG CCGGGAACGG 841 TGCATTGGAA CGCGGATTCC CCGTGCCAAG AGTGACGTAA GTACCGCCTA TAGAGTCTAT 901 AGGCCCACAA AAAATGCTTT CTTCTTTTAA TATACTTTTT TGTTTATCTT ATTTCTAATA 961 CTTTCCCTAA TCTCTTTCTT TCAGGGCAAT AATGATACAA TGTATCATGC CTCTTTGCAC 1021 CATTCTAAAG AATAACAGTG ATAATTTCTG GGTTAAGGCA ATAGCAATAT TTCTGCATAT 1081 AAATATTTCT GCATATAAAT TGTAACTGAT GTAAGAGGTT TCATATTGCT AATAGCAGCT 1141 ACAATCCAGC TACCATTCTG CTTTTATTTT ATGGTTGGGA TAAGGCTGGA TTATTCTGAG 1201 TCCAAGCTAG GCCCTTTTGC TAATCATGTT CATACCTCTT ATCTTCCTCC CACAGCTCCT 1261 GGGCAACGTG CTGGTCTGTG TGCTGGCCCA TCACTTTGGC AAAGAATTGG GATTCGAACA 1321 TCGATTGAAT TCCACCATGG CCCAGTCCAA GCACGGCCTG ACCAAGGAGA TGACCATGAA 1381 GTACCGCATG GAGGGCTGCG TGGACGGCCA CAAGTTCGTG ATCACCGGCG AGGGCATCGG 1441 CTACCCCTTC AAGGGCAAGC AGGCCATCAA CCTGTGCGTG GTGGAGGGCG GCCCCTTGCC 1501 CTTCGCCGAG GACATCTTGT CCGCCGCCTT CATGTACGGC AACCGCGTGT TCACCGAGTA 1561 CCCCCAGGAC ATCGTCGACT ACTTCAAGAA CTCCTGCCCC GCCGGCTACA CCTGGGACCG 1621 CTCCTTCCTG TTCGAGGACG GCGCCGTGTG CATCTGCAAC GCCGACATCA CCGTGAGCGT 1681 GGAGGAGAAC TGCATGTACC ACGAGTCCAA GTTCTACGGC GTGAACTTCC CCGCCGACGG 1741 CCCCGTGATG AAGAAGATGA CCGACAACTG GGAGCCCTCC TGCGAGAAGA TCATCCCCGT 1801 GCCCAAGCAG GGCATCTTGA AGGGCGACGT GAGCATGTAC CTGCTGCTGA AGGACGGTGG 1861 CCGCTTGCGC TGCCAGTTCG ACACCGTGTA CAAGGCCAAG TCCGTGCCCC GCAAGATGCC 1921 CGACTGGCAC TTCATCCAGC ACAAGCTGAC CCGCGAGGAC CGCAGCGACG CCAAGAACCA 1981 GAAGTGGCAC CTGACCGAGC ACGCCATCGC CTCCGGCTCC GCCTTGCCCT AAAGATCTAC 2041 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca 2101 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc 2161 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca 2221 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg 2281 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg 2341 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg 2401 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct 2461 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg 2521 attttgtagg taaccacgtg cggaccTCGA AATTCCCCAG TGGAAAGACG CGCAGGCAAA 2581 ACGCACCACG TGACGGAGCG TGACCGCGCG CCGAGCGCGC GCCAAGGTCG GGCAGGAAGA 2641 GGGCCTATTT CCCATGATTC CTTCATATTT GCATATACGA TACAAGGCTG TTAGAGAGAT 2701 AATTAGAATT AATTTGACTG TAAACACAAA GATATTAGTA CAAAATACGT GACGTAGAAA 2761 GTAATAATTT CTTGGGTAGT TTGCAGTTTT AAAATTATGT TTTAAAATGG ACTATCATAT 2821 GCTTACCGTA ACTTGAAAGT ATTTCGATTT CTTGGGTTTA TATATCTTGT GGAAAGGACG 2881 CGGGATCCGG GATATCTTCC GGTTCATAGA ctcgagTCTA TGAACCGGAA GATATCCttt 2941 tttAAGCTTG CGGccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc 3001 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc 3061 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct 3121 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc 3181 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 3241 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 3301 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 3361 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc 3421 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 3481 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt 3541 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 3601 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat 3661 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 3721 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 3781 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta 3841 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 3901 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 3961 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 4021 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 4081 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 4141 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 4201 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 4261 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 4321 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 4381 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 4441 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 4501 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 4561 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 4621 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 4681 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 4741 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 4801 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 4861 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 4921 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 4981 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 5041 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 5101 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 5161 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 5221 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 5281 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 5341 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 5401 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 5461 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 5521 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 5581 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 5641 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.