pLV3-EF1a-MCS-IRES-Puro plasmi

pLV3-EF1a-MCS-IRES-Puro plasmid

PVT91268

pLV3-EF1a-MCS-IRES-Puro plasmid Information

Plasmid name: pLV3-EF1a-MCS-IRES-Puro
Alias: NM_001108059.3/NM_001432739.1;AABR07015003.1; Klb-ps1; RGD1308227;
Gene lenght: 1284,3132,bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEF-F
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-MCS-IRES-Puro plasmid Description

pLV3-EF1a-MCS-IRES-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-EF1a-MCS-IRES-Puro plasmid Sequence

1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg 3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag 3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag 3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt 3301 caggtgtcgt gaaaactacc cctctagata atacgactca ctatagggcc cgggttacgc 3361 gtctcgagac tagttctaga gcggccgcgg atcccgcccc tctccctccc ccccccctaa 3421 cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc 3481 caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac 3541 gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt 3601 gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg 3661 caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata 3721 agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga 3781 aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt 3841 accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc 3901 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac 3961 acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga gtacaagccc 4021 acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg 4081 ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc 4141 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc 4201 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg 4261 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag 4321 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc 4381 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc 4441 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc 4501 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa 4561 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct aggtcgacaa tcaacctctg 4621 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 4681 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 4741 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 4801 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 4861 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 4921 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 4981 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 5041 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 5101 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct 5161 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt 5221 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 5281 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt 5341 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 5401 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 5461 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 5521 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 5581 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 5641 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 5701 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct 5761 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 5821 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 5881 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc 5941 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca 6001 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc 6061 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg 6121 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct 6181 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta 6241 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa 6301 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct 6361 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc 6421 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg 6481 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt 6541 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 6601 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 6661 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 6721 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 6781 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 6841 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 6901 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact 6961 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 7021 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 7081 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 7141 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 7201 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg 7261 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 7321 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 7381 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 7441 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 7501 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 7561 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 7621 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 7681 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 7741 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 7801 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc 7861 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 7921 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 7981 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 8041 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 8101 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 8161 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 8221 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 8281 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 8341 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 8401 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 8461 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 8521 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg 8581 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 8641 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc 8701 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgc

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.