pLV2-Ubc-MCS-3×FLAG-IRES-EGFP-

pLV2-Ubc-MCS-3×FLAG-IRES-EGFP-Puro plasmid

PVT93968

pLV2-Ubc-MCS-3×FLAG-IRES-EGFP-Puro plasmid Information

Plasmid name: pLV2-Ubc-MCS-3×FLAG-IRES-EGFP-Puro
Alias:
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: Ubc
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: UbC-F2
Reverse primer:
Copy number: High
Induction:

pLV2-Ubc-MCS-3×FLAG-IRES-EGFP-Puro plasmid Description

pLV2-Ubc-MCS-3×FLAG-IRES-EGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-Ubc-MCS-3×FLAG-IRES-EGFP-Puro plasmid Sequence

1 TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG ATCTACCACA 61 CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC AGATATCCAC 121 TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA GAAGAGGCCA 181 ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG GATGACCCGG 241 AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC GTGGCCCGAG 301 AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG GGACTTTCCG 361 CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA GCCCTCAGAT 421 CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA 481 GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT 541 TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC 601 AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG 661 CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG 721 CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA 781 AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG 841 AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG 901 CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT 961 GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT 1021 CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA 1081 CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC 1141 AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT ATGAGGGACA ATTGGAGAAG 1201 TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA GGAGTAGCAC CCACCAAGGC 1261 AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA ATAGGAGCTT TGTTCCTTGG 1321 GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA ATGACGCTGA CGGTACAGGC 1381 CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT TTGCTGAGGG CTATTGAGGC 1441 GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG CAGCTCCAGG CAAGAATCCT 1501 GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG ATTTGGGGTT GCTCTGGAAA 1561 ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG AGTAATAAAT CTCTGGAACA 1621 GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA ATTAACAATT ACACAAGCTT 1681 AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA AAGAATGAAC AAGAATTATT 1741 GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC ATAACAAATT GGCTGTGGTA 1801 TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT TTAAGAATAG TTTTTGCTGT 1861 ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA TTATCGTTTC AGACCCACCT 1921 CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA GAAGAAGGTG GAGAGAGAGA 1981 CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCTCGACGG TATCGCCTTT AAAAGAAAAG 2041 GGGGGATTGG GGGGTACAGT GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC 2101 AAACTAAAGA ATTACAAAAA CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG 2161 ACAGCAGAGA TCCAGTTTAT CGATGGCCTC CGCGCCGGGT TTTGGCGCCT CCCGCGGGCG 2221 CCCCCCTCCT CACGGCGAGC GCTGCCACGT CAGACGAAGG GCGCAGCGAG CGTCCTGATC 2281 CTTCCGCCCG GACGCTCAGG ACAGCGGCCC GCTGCTCATA AGACTCGGCC TTAGAACCCC 2341 AGTATCAGCA GAAGGACATT TTAGGACGGG ACTTGGGTGA CTCTAGGGCA CTGGTTTTCT 2401 TTCCAGAGAG CGGAACAGGC GAGGAAAAGT AGTCCCTTCT CGGCGATTCT GCGGAGGGAT 2461 CTCCGTGGGG CGGTGAACGC CGATGATTAT ATAAGGACGC GCCGGGTGTG GCACAGCTAG 2521 TTCCGTCGCA GCCGGGATTT GGGTCGCGGT TCTTGTTTGT GGATCGCTGT GATCGTCACT 2581 TGGTGAGTAG CGGGCTGCTG GGCTGGCCGG GGCTTTCGTG GCCGCCGGGC CGCTCGGTGG 2641 GACGGAAGCG TGTGGAGAGA CCGCCAAGGG CTGTAGTCTG GGTCCGCGAG CAAGGTTGCC 2701 CTGAACTGGG GGTTGGGGGG AGCGCAGCAA AATGGCGGCT GTTCCCGAGT CTTGAATGGA 2761 AGACGCTTGT GAGGCGGGCT GTGAGGTCGT TGAAACAAGG TGGGGGGCAT GGTGGGCGGC 2821 AAGAACCCAA GGTCTTGAGG CCTTCGCTAA TGCGGGAAAG CTCTTATTCG GGTGAGATGG 2881 GCTGGGGCAC CATCTGGGGA CCCTGACGTG AAGTTTGTCA CTGACTGGAG AACTCGGGTT 2941 TGTCGTCTGG TTGCGGGGGC GGCAGTTATG CGGTGCCGTT GGGCAGTGCA CCCGTACCTT 3001 TGGGAGCGCG CGCCTCGTCG TGTCGTGACG TCACCCGTTC TGTTGGCTTA TAATGCAGGG 3061 TGGGGCCACC TGCCGGTAGG TGTGCGGTAG GCTTTTCTCC GTCGCAGGAC GCAGGGTTCG 3121 GGCCTAGGGT AGGCTCTCCT GAATCGACAG GCGCCGGACC TCTGGTGAGG GGAGGGATAA 3181 GTGAGGCGTC AGTTTCTTTG GTCGGTTTTA TGTACCTATC TTCTTAAGTA GCTGAAGCTC 3241 CGGTTTTGAA CTATGCGCTC GGGGTTGGCG AGTGTGTTTT GTGAAGTTTT TTAGGCACCT 3301 TTTGAAATGT AATCATTTGG GTCAATATGT AATTTTCAGT GTTAGACTAG TAAATTGTCC 3361 GCTAAATTCT GGCCGTTTTT GGCTTTTTTG TTAGACCTCG AGCTCAAGCT TCGAATTCtg 3421 cagtcgacgg taccgcgggc ccgggatcca tgGACTACAA AGACCATGAC GGTGATTATA 3481 AAGATCATGA CATCGATTAC AAGGATGACG ACGATAAGtg aggatccccc ctctccctcc 3541 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat 3601 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct 3661 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg 3721 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta 3781 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag 3841 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg 3901 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat 3961 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca 4021 tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc 4081 ctttgaaaaa cacgatgata agcttgccac aacccATGGT GAGCAAGGGC GAGGAGCTGT 4141 TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA 4201 GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT 4261 GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTACGGCG 4321 TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA 4381 TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA 4441 CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA 4501 TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC TACAACAGCC 4561 ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC TTCAAGATCC 4621 GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG AACACCCCCA 4681 TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG TCCGCCCTGA 4741 GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG 4801 GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAGGCGGC CGCGACTCTA GATAATTCTA 4861 CCGGGTAGGG GAGGCGCTTT TCCCAAGGCA GTCTGGAGCA TGCGCTTTAG CAGCCCCGCT 4921 GGGCACTTGG CGCTACACAA GTGGCCTCTG GCCTCGCACA CATTCCACAT CCACCGGTAG 4981 GCGCCAACCG GCTCCGTTCT TTGGTGGCCC CTTCGCGCCA CCTTCTACTC CTCCCCTAGT 5041 CAGGAAGTTC CCCCCCGCCC CGCAGCTCGC GTCGTGCAGG ACGTGACAAA TGGAAGTAGC 5101 ACGTCTCACT AGTCTCGTGC AGATGGACAG CACCGCTGAG CAATGGAAGC GGGTAGGCCT 5161 TTGGGGCAGC GGCCAATAGC AGCTTTGCTC CTTCGCTTTC TGGGCTCAGA GGCTGGGAAG 5221 GGGTGGGTCC GGGGGCGGGC TCAGGGGCGG GCTCAGGGGC GGGGCGGGCG CCCGAAGGTC 5281 CTCCGGAGGC CCGGCATTCT GCACGCTTCA AAAGCGCACG TCTGCCGCGC TGTTCTCCTC 5341 TTCCTCATCT CCGGGCCTTT CGACCTGCAG CCCAAGCTTA CCATGACCGA GTACAAGCCC 5401 ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCAGGGCCG TACGCACCCT CGCCGCCGCG 5461 TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGATCCGG ACCGCCACAT CGAGCGGGTC 5521 ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC 5581 GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG 5641 GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG 5701 CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC 5761 ACCGTCGGCG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC 5821 GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAGACCTC CGCGCCCCGC 5881 AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA 5941 GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCCT GACCGCGTCT GGAACAATCA 6001 ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG TTGCTCCTTT 6061 TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT CCCGTATGGC 6121 TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG AGTTGTGGCC 6181 CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC CCACTGGTTG 6241 GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC TCCCTATTGC 6301 CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG 6361 CACTGACAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCC TTTCCATGGC TGCTCGCCTG 6421 TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG CCCTCAATCC 6481 AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT 6541 TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCCTGGAA TTAATTCTGC 6601 AGTCGAGACC TAGAAAAACA TGGAGCAATC ACAAGTAGCA ATACAGCAGC TACCAATGCT 6661 GATTGTGCCT GGCTAGAAGC ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT CACACCTCAG 6721 GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT TTTAAAAGAA 6781 AAGAGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATATCCT TGATCTGTGG 6841 ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC 6901 AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA 6961 GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG 7021 GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC 7081 GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG 7141 GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA 7201 GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA 7261 CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA 7321 AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA 7381 GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTAGTA GTTCATGTCA 7441 TCTTATTATT CAGTATTTAT AACTTGCAAA GAAATGAATA TCAGAGAGTG AGAGGCCTTG 7501 ACATTGCTAG CGTTTTACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC 7561 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 7621 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 7681 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 7741 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 7801 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 7861 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 7921 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 7981 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 8041 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 8101 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 8161 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 8221 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 8281 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 8341 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG 8401 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 8461 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA 8521 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 8581 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 8641 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 8701 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 8761 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 8821 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 8881 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 8941 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 9001 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 9061 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 9121 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 9181 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 9241 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 9301 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 9361 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 9421 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 9481 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 9541 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 9601 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 9661 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC GACGGATCGG 9721 GAGATCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG CATCACAAAT 9781 TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA ACTCATCAAT 9841 GTATCTTATC ATGTCTGGAT CAACTGGATA ACTCAAGCTA ACCAAAATCA TCCCAAACTT 9901 CCCACCCCAT ACCCTATTAC CACTGCCAAT TACCTGTGGT TTCATTTACT CTAAACCTGT 9961 GATTCCTCTG AATTATTTTC ATTTTAAAGA AATTGTATTT GTTAAATATG TACTACAAAC 10021 TTAGTAGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.