pCMV-3×HA-Rab8a-Neo plasmid

pCMV-3×HA-Rab8a-Neo plasmid

PVT94829

pCMV-3×HA-Rab8a-Neo plasmid Information

Plasmid name: pCMV-3×HA-Rab8a(mouse)-Neo
Alias: NM_023126.2;AA409338; Mel
Gene lenght: 624bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-3×HA-Rab8a-Neo plasmid Description

pCMV-3×HA-Rab8a-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-3×HA-Rab8a-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttAAACTT AAGCCACCAT GGCGGCGTAC CCATACGACG TACCTGATTA TGCAACTTAT 961 CCTTATGATG TACCTGATTA TGCTAGCTAT CCTTATGATG TCCCAGACTA CGCATCAGCG 1021 GAAAAGCTTa tggcgaagac ctacgattac ctgttcaagc tgctgctgat cggggactcg 1081 ggggtaggga agacctgtgt cctgttccgc ttctccgagg acgccttcaa ctccacattc 1141 atctctacca taggaattga ctttaaaatt aggaccatag agctcgatgg caagaggatt 1201 aaactgcaga tatgggacac ggccggccag gagcggtttc gaacaatcac gacagcctac 1261 tacaggggtg ccatgggtat catgctggtc tacgacatta ccaatgagaa gtcctttgac 1321 aacatccgga attggattcg gaacattgaa gagcatgcct ctgcagacgt ggagaagatg 1381 atactgggga ataagtgtga tgtgaatgac aagagacagg tgtccaagga acggggagaa 1441 aagctggcac tcgactatgg gatcaagttc atggagacca gtgcaaaggc caacatcaat 1501 gtggagaatg catttttcac tcttgccagg gatatcaaag caaaaatgga caaaaaattg 1561 gaagggaaca gcccgcaggg gagcagccat ggagtcaaga tcacagtgga gcagcagaag 1621 aggaccagct tcttccggtg cagtctcctg tgagaattct gcagatatcc agcacagtgg 1681 cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct 1741 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc 1801 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt 1861 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat 1921 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg 1981 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 2041 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 2101 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 2161 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 2221 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 2281 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 2341 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 2401 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg 2461 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 2521 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 2581 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 2641 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg 2701 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 2761 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat caagagacag 2821 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt 2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg 2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg 3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg 3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg 3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca 3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc 3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc 3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca 3361 aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga 3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg 3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg 3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg 3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga 3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag 3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct 3781 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata 3841 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 3901 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag 3961 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 4021 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 4081 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 4141 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 4201 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 4261 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 4321 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 4381 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 4441 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 4501 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 4561 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 4621 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 4681 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 4741 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 4801 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 4861 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtttttt 4921 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 4981 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 5041 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 5101 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 5161 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 5221 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 5281 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 5341 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 5401 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 5461 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 5521 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 5581 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 5641 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 5701 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 5761 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 5821 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 5881 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 5941 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 6001 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 6061 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 6121 acctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.