pCas-EGFP plasmid

pCas-EGFP plasmid

PVT91182

pCas-EGFP plasmid Information

Plasmid name: pCas(#62225)-EGFP
Alias: -
Gene lenght:
Host: E.coli
Use(s): Gene editing
Fragment type: CRISPR,Cas9
Species: Empty Vector
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: araBAD
Replicon: pSC101
Growth Strain(s): Stbl3
Growth Temperature: 30°C
Vector backbone:
Forward primer: Cas9-F1
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pCas-EGFP plasmid Description

pCas-EGFP is a plasmid suitable for gene editing in Escherichia coli.

pCas-EGFP plasmid Sequence

1 AAAGCATATT AAACTAATTT CGGAGGTCAT TAAAACTATT ATTGAAATCA TCAAACTCAT 61 TATGGATTTA ATTTAAACTT TTTATTTTAG GAGGCAAAAA TGGATAAGAA ATACTCAATA 121 GGCTTAGATA TCGGCACAAA TAGCGTCGGA TGGGCGGTGA TCACTGATGA ATATAAGGTT 181 CCGTCTAAAA AGTTCAAGGT TCTGGGAAAT ACAGACCGCC ACAGTATCAA AAAAAATCTT 241 ATAGGGGCTC TTTTATTTGA CAGTGGAGAG ACAGCGGAAG CGACTCGTCT CAAACGGACA 301 GCTCGTAGAA GGTATACACG TCGGAAGAAT CGTATTTGTT ATCTACAGGA GATTTTTTCA 361 AATGAGATGG CGAAAGTAGA TGATAGTTTC TTTCATCGAC TTGAAGAGTC TTTTTTGGTG 421 GAAGAAGACA AGAAGCATGA ACGTCATCCT ATTTTTGGAA ATATAGTAGA TGAAGTTGCT 481 TATCATGAGA AATATCCAAC TATCTATCAT CTGCGAAAAA AATTGGTAGA TTCTACTGAT 541 AAAGCGGATT TGCGCTTAAT CTATTTGGCC TTAGCGCATA TGATTAAGTT TCGTGGTCAT 601 TTTTTGATTG AGGGAGATTT AAATCCTGAT AATAGTGATG TGGACAAACT ATTTATCCAG 661 TTGGTACAAA CCTACAATCA ATTATTTGAA GAAAACCCTA TTAACGCAAG TGGAGTAGAT 721 GCTAAAGCGA TTCTTTCTGC ACGATTGAGT AAATCAAGAC GATTAGAAAA TCTCATTGCT 781 CAGCTCCCCG GTGAGAAGAA AAATGGCTTA TTTGGGAATC TCATTGCTTT GTCATTGGGT 841 TTGACCCCTA ATTTTAAATC AAATTTTGAT TTGGCAGAAG ATGCTAAATT ACAGCTTTCA 901 AAAGATACTT ACGATGATGA TTTAGATAAT TTATTGGCGC AAATTGGAGA TCAATATGCT 961 GATTTGTTTT TGGCAGCTAA GAATTTATCA GATGCTATTT TACTTTCAGA TATCCTAAGA 1021 GTAAATACTG AAATAACTAA GGCTCCCCTA TCAGCTTCAA TGATTAAACG CTACGATGAA 1081 CATCATCAAG ACTTGACTCT TTTAAAAGCT TTAGTTCGAC AACAACTTCC AGAAAAGTAT 1141 AAAGAAATCT TTTTTGATCA ATCAAAAAAC GGATATGCAG GTTATATTGA TGGGGGAGCT 1201 AGCCAAGAAG AATTTTATAA ATTTATCAAA CCAATTTTAG AAAAAATGGA TGGTACTGAG 1261 GAATTATTGG TGAAACTAAA TCGTGAAGAT TTGCTGCGCA AGCAACGGAC CTTTGACAAC 1321 GGCTCTATTC CCCATCAAAT TCACTTGGGT GAGCTGCATG CTATTTTGAG AAGACAAGAA 1381 GACTTTTATC CATTTTTAAA AGACAATCGT GAGAAGATTG AAAAAATCTT GACTTTTCGA 1441 ATTCCTTATT ATGTTGGTCC ATTGGCGCGT GGCAATAGTC GTTTTGCATG GATGACTCGG 1501 AAGTCTGAAG AAACAATTAC CCCATGGAAT TTTGAAGAAG TTGTCGATAA AGGTGCTTCA 1561 GCTCAATCAT TTATTGAACG CATGACAAAC TTTGATAAAA ATCTTCCAAA TGAAAAAGTA 1621 CTACCAAAAC ATAGTTTGCT TTATGAGTAT TTTACGGTTT ATAACGAATT GACAAAGGTC 1681 AAATATGTTA CTGAAGGAAT GCGAAAACCA GCATTTCTTT CAGGTGAACA GAAGAAAGCC 1741 ATTGTTGATT TACTCTTCAA AACAAATCGA AAAGTAACCG TTAAGCAATT AAAAGAAGAT 1801 TATTTCAAAA AAATAGAATG TTTTGATAGT GTTGAAATTT CAGGAGTTGA AGATAGATTT 1861 AATGCTTCAT TAGGTACCTA CCATGATTTG CTAAAAATTA TTAAAGATAA AGATTTTTTG 1921 GATAATGAAG AAAATGAAGA TATCTTAGAG GATATTGTTT TAACATTGAC CTTATTTGAA 1981 GATAGGGAGA TGATTGAGGA AAGACTTAAA ACATATGCTC ACCTCTTTGA TGATAAGGTG 2041 ATGAAACAGC TTAAACGTCG CCGTTATACT GGTTGGGGAC GTTTGTCTCG AAAATTGATT 2101 AATGGTATTA GGGATAAGCA ATCTGGCAAA ACAATATTAG ATTTTTTGAA ATCAGATGGT 2161 TTTGCCAATC GCAATTTTAT GCAGCTGATC CATGATGATA GTTTGACATT TAAAGAAGAC 2221 ATTCAAAAAG CACAAGTGTC TGGACAAGGC GATAGTTTAC ATGAACATAT TGCAAATTTA 2281 GCTGGTAGCC CTGCTATTAA AAAAGGTATT TTACAGACTG TAAAAGTTGT TGATGAATTG 2341 GTCAAAGTAA TGGGGCGGCA TAAGCCAGAA AATATCGTTA TTGAAATGGC ACGTGAAAAT 2401 CAGACAACTC AAAAGGGCCA GAAAAATTCG CGAGAGCGTA TGAAACGAAT CGAAGAAGGT 2461 ATCAAAGAAT TAGGAAGTCA GATTCTTAAA GAGCATCCTG TTGAAAATAC TCAATTGCAA 2521 AATGAAAAGC TCTATCTCTA TTATCTCCAA AATGGAAGAG ACATGTATGT GGACCAAGAA 2581 TTAGATATTA ATCGTTTAAG TGATTATGAT GTCGATCACA TTGTTCCACA AAGTTTCCTT 2641 AAAGACGATT CAATAGACAA TAAGGTCTTA ACGCGTTCTG ATAAAAATCG TGGTAAATCG 2701 GATAACGTTC CAAGTGAAGA AGTAGTCAAA AAGATGAAAA ACTATTGGAG ACAACTTCTA 2761 AACGCCAAGT TAATCACTCA ACGTAAGTTT GATAATTTAA CGAAAGCTGA ACGTGGAGGT 2821 TTGAGTGAAC TTGATAAAGC TGGTTTTATC AAACGCCAAT TGGTTGAAAC TCGCCAAATC 2881 ACTAAGCATG TGGCACAAAT TTTGGATAGT CGCATGAATA CTAAATACGA TGAAAATGAT 2941 AAACTTATTC GAGAGGTTAA AGTGATTACC TTAAAATCTA AATTAGTTTC TGACTTCCGA 3001 AAAGATTTCC AATTCTATAA AGTACGTGAG ATTAACAATT ACCATCATGC CCATGATGCG 3061 TATCTAAATG CCGTCGTTGG AACTGCTTTG ATTAAGAAAT ATCCAAAACT TGAATCGGAG 3121 TTTGTCTATG GTGATTATAA AGTTTATGAT GTTCGTAAAA TGATTGCTAA GTCTGAGCAA 3181 GAAATAGGCA AAGCAACCGC AAAATATTTC TTTTACTCTA ATATCATGAA CTTCTTCAAA 3241 ACAGAAATTA CACTTGCAAA TGGAGAGATT CGCAAACGCC CTCTAATCGA AACTAATGGG 3301 GAAACTGGAG AAATTGTCTG GGATAAAGGG CGAGATTTTG CCACAGTGCG CAAAGTATTG 3361 TCCATGCCCC AAGTCAATAT TGTCAAGAAA ACAGAAGTAC AGACAGGCGG ATTCTCCAAG 3421 GAGTCAATTT TACCAAAAAG AAATTCGGAC AAGCTTATTG CTCGTAAAAA AGACTGGGAT 3481 CCAAAAAAAT ATGGTGGTTT TGATAGTCCA ACGGTAGCTT ATTCAGTCCT AGTGGTTGCT 3541 AAGGTGGAAA AAGGGAAATC GAAGAAGTTA AAATCCGTTA AAGAGTTACT AGGGATCACA 3601 ATTATGGAAA GAAGTTCCTT TGAAAAAAAT CCGATTGACT TTTTAGAAGC TAAAGGATAT 3661 AAGGAAGTTA AAAAAGACTT AATCATTAAA CTACCTAAAT ATAGTCTTTT TGAGTTAGAA 3721 AACGGTCGTA AACGGATGCT GGCTAGTGCC GGAGAATTAC AAAAAGGAAA TGAGCTGGCT 3781 CTGCCAAGCA AATATGTGAA TTTTTTATAT TTAGCTAGTC ATTATGAAAA GTTGAAGGGT 3841 AGTCCAGAAG ATAACGAACA AAAACAATTG TTTGTGGAGC AGCATAAGCA TTATTTAGAT 3901 GAGATTATTG AGCAAATCAG TGAATTTTCT AAGCGTGTTA TTTTAGCAGA TGCCAATTTA 3961 GATAAAGTTC TTAGTGCATA TAACAAACAT AGAGACAAAC CAATACGTGA ACAAGCAGAA 4021 AATATTATTC ATTTATTTAC GTTGACGAAT CTTGGAGCTC CCGCTGCTTT TAAATATTTT 4081 GATACAACAA TTGATCGTAA ACGATATACG TCTACAAAAG AAGTTTTAGA TGCCACTCTT 4141 ATCCATCAAT CCATCACTGG TCTTTATGAA ACACGCATTG ATTTGAGTCA GCTAGGAGGT 4201 GACGGCGGTG GAGGAAGCgg cggaggtgga tctatggtga gcaagggcga ggagctgttc 4261 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc 4321 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc 4381 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg 4441 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg 4501 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc 4561 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc 4621 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac 4681 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc 4741 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc 4801 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc 4861 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg 4921 atcactctcg gcatggacga gctgtacaag tagAGTATAT TTTAGATGAA GATTATTTCT 4981 TAATCTAGAC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT 5041 TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGCATCGATT TATTATGACA ACTTGACGGC 5101 TACATCATTC ACTTTTTCTT CACAACCGGC ACGGAACTCG CTCGGGCTGG CCCCGGTGCA 5161 TTTTTTAAAT ACCCGCGAGA AGTAGAGTTG ATCGTCAAAA CCAACATTGC GACCGACGGT 5221 GGCGATAGGC ATCCGGGTGG TGCTCAAAAG CAGCTTCGCC TGGCTGATAC GTTGGTCCTC 5281 GCGCCAGCTT AAGACGCTAA TCCCTAACTG CTGGCGGAAA AGATGTGACA GACGCGACGG 5341 CGACAAGCAA ACATGCTGTG CGACGCTGGC GATATCAAAA TTGCTGTCTG CCAGGTGATC 5401 GCTGATGTAC TGACAAGCCT CGCGTACCCG ATTATCCATC GGTGGATGGA GCGACTCGTT 5461 AATCGCTTCC ATGTGCCGCA GTAACAATTG CTCAAGCAGA TTTATCGCCA GCAGCTCCGA 5521 ATAGCGCCCT TCCCCTTGCC CGGCGTTAAT GATTTGCCCA AACAGGTCGC TGAAATGCGG 5581 CTGGTGCGCT TCATCCGGGC GAAAGAACCC CGTATTGGCA AATATTGACG GCCAGTTAAG 5641 CCATTCATGC CAGTAGGCGC GCGGACGAAA GTAAACCCAC TGGTGATACC ATTCGCGAGC 5701 CTCCGGATGA CGACCGTAGT GATGAATCTC TCCTGGCGGG AACAGCAAAA TATCACCCGG 5761 TCGGCAAACA AATTCTCGTC CCTGATTTTT CACCACCCCC TGACCGCGAA TGGTGAGATT 5821 GAGAATATAA CCTTTCATTC CCAGCGGTCG GTCGATAAAA AAATCGAGAT AACCGTTGGC 5881 CTCAATCGGC GTTAAACCCG CCACCAGATG GGCATTAAAC GAGTATCCCG GCAGCAGGGG 5941 ATCATTTTGC GCTTCAGCCA TACTTTTCAT ACTCCCGCCA TTCAGAGAAG AAACCAATTG 6001 TCCATATTGC ATCAGACATT GCCGTCACTG CGTCTTTTAC TGGCTCTTCT CGCTAACCAA 6061 ACCGGTAACC CCGCTTATTA AAAGCATTCT GTAACAAAGC GGGACCAAAG CCATGACAAA 6121 AACGCGTAAC AAAAGTGTCT ATAATCACGG CAGAAAAGTC CACATTGATT ATTTGCACGG 6181 CGTCACACTT TGCTATGCCA TAGCATTTTT ATCCATAAGA TTAGCGGATC CTACCTGACG 6241 CTTTTTATCG CAACTCTCTA CTGTTTCTCC ATACCCGTTT TTTTGGGAAT TCGAGCTCTA 6301 AGGAGGTTAT AAAAAATGGA TATTAATACT GAAACTGAGA TCAAGCAAAA GCATTCACTA 6361 ACCCCCTTTC CTGTTTTCCT AATCAGCCCG GCATTTCGCG GGCGATATTT TCACAGCTAT 6421 TTCAGGAGTT CAGCCATGAA CGCTTATTAC ATTCAGGATC GTCTTGAGGC TCAGAGCTGG 6481 GCGCGTCACT ACCAGCAGCT CGCCCGTGAA GAGAAAGAGG CAGAACTGGC AGACGACATG 6541 GAAAAAGGCC TGCCCCAGCA CCTGTTTGAA TCGCTATGCA TCGATCATTT GCAACGCCAC 6601 GGGGCCAGCA AAAAATCCAT TACCCGTGCG TTTGATGACG ATGTTGAGTT TCAGGAGCGC 6661 ATGGCAGAAC ACATCCGGTA CATGGTTGAA ACCATTGCTC ACCACCAGGT TGATATTGAT 6721 TCAGAGGTAT AAAACGAATG AGTACTGCAC TCGCAACGCT GGCTGGGAAG CTGGCTGAAC 6781 GTGTCGGCAT GGATTCTGTC GACCCACAGG AACTGATCAC CACTCTTCGC CAGACGGCAT 6841 TTAAAGGTGA TGCCAGCGAT GCGCAGTTCA TCGCATTACT GATCGTTGCC AACCAGTACG 6901 GCCTTAATCC GTGGACGAAA GAAATTTACG CCTTTCCTGA TAAGCAGAAT GGCATCGTTC 6961 CGGTGGTGGG CGTTGATGGC TGGTCCCGCA TCATCAATGA AAACCAGCAG TTTGATGGCA 7021 TGGACTTTGA GCAGGACAAT GAATCCTGTA CATGCCGGAT TTACCGCAAG GACCGTAATC 7081 ATCCGATCTG CGTTACCGAA TGGATGGATG AATGCCGCCG CGAACCATTC AAAACTCGCG 7141 AAGGCAGAGA AATCACGGGG CCGTGGCAGT CGCATCCCAA ACGGATGTTA CGTCATAAAG 7201 CCATGATTCA GTGTGCCCGT CTGGCCTTCG GATTTGCTGG TATCTATGAC AAGGATGAAG 7261 CCGAGCGCAT TGTCGAAAAT ACTGCATACA CTGCAGAACG TCAGCCGGAA CGCGACATCA 7321 CTCCGGTTAA CGATGAAACC ATGCAGGAGA TTAACACTCT GCTGATCGCC CTGGATAAAA 7381 CATGGGATGA CGACTTATTG CCGCTCTGTT CCCAGATATT TCGCCGCGAC ATTCGTGCAT 7441 CGTCAGAACT GACACAGGCC GAAGCAGTAA AAGCTCTTGG ATTCCTGAAA CAGAAAGCCG 7501 CAGAGCAGAA GGTGGCAGCA TGACACCGGA CATTATCCTG CAGCGTACCG GGATCGATGT 7561 GAGAGCTGTC GAACAGGGGG ATGATGCGTG GCACAAATTA CGGCTCGGCG TCATCACCGC 7621 TTCAGAAGTT CACAACGTGA TAGCAAAACC CCGCTCCGGA AAGAAGTGGC CTGACATGAA 7681 AATGTCCTAC TTCCACACCC TGCTTGCTGA GGTTTGCACC GGTGTGGCTC CGGAAGTTAA 7741 CGCTAAAGCA CTGGCCTGGG GAAAACAGTA CGAGAACGAC GCCAGAACCC TGTTTGAATT 7801 CACTTCCGGC GTGAATGTTA CTGAATCCCC GATCATCTAT CGCGACGAAA GTATGCGTAC 7861 CGCCTGCTCT CCCGATGGTT TATGCAGTGA CGGCAACGGC CTTGAACTGA AATGCCCGTT 7921 TACCTCCCGG GATTTCATGA AGTTCCGGCT CGGTGGTTTC GAGGCCATAA AGTCAGCTTA 7981 CATGGCCCAG GTGCAGTACA GCATGTGGGT GACGCGAAAA AATGCCTGGT ACTTTGCCAA 8041 CTATGACCCG CGTATGAAGC GTGAAGGCCT GCATTATGTC GTGATTGAGC GGGATGAAAA 8101 GTACATGGCG AGTTTTGACG AGATCGTGCC GGAGTTCATC GAAAAAATGG ACGAGGCACT 8161 GGCTGAAATT GGTTTTGTAT TTGGGGAGCA ATGGCGATGA CGCATCCTCA CGATAATATC 8221 CGGGTAGGCG CAATCACTTT CGTCTACTCC GTTACAAAGC GAGGCTGGGT ATTTCCCGGC 8281 CTTTCTGTTA TCCGAAATCC ACTGAAAGCA CAGCGGCTGG CTGAGGAGAT AAATAATAAA 8341 CGAGGGGCTG TATGCACAAA GCATCTTCTG TTGAGTTAAG AACGAGTATC GAGATGGCAC 8401 ATAGCCTTGC TCAAATTGGA ATCAGGTTTG TGCCAATACC AGTAGAAACA GACGAAGAAT 8461 CCATGGGTAT GGACAGATCT CAAAAAAAGC ACCGACTCGG TGCCACTTTT TCAAGTTGAT 8521 AACGGACTAG CCTTATTTTA ACTTGCTATT TCTAGCTCTA AAACCTGGTA ACAGGATTAG 8581 CAGATGTGTG AAATTGTTAT CCGCTCACAA TTCCACACAA CATACGAGCC GGATGATTAA 8641 TTGTCAACAG CTCATTTCAG AATATTTGCC AGAACCGTTA TGATGTCGGC GCAAAAAACA 8701 TTATCCAGAA CGGGAGTGCG CCTTGAGCGA CACGAATTAT GCAGTGATTT ACGACCTGCA 8761 CAGCCATACC ACAGCTTCCG ATGGCTGCCT GACGCCAGAA GCATTGGTGC ACCGTGCAGT 8821 CGATGATAAG CTGTCAAACC AGATCAATTC GCGCTAACTT ACATTAATTG CGTTGCGCTC 8881 ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG 8941 CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA 9001 CGGGCAACAG CTGATTGCCC TTCACCGCCT GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA 9061 CGCTGGTTTG CCCCAGCAGG CGAAAATCCT GTTTGATGGT GGTTAACGGC GGGATATAAC 9121 ATGAGCTGTC TTCGGTATCG TCGTATCCCA CTACCGAGAT ATCCGCACCA ACGCGCAGCC 9181 CGGACTCGGT AATGGCGCGC ATTGCGCCCA GCGCCATCTG ATCGTTGGCA ACCAGCATCG 9241 CAGTGGGAAC GATGCCCTCA TTCAGCATTT GCATGGTTTG TTGAAAACCG GACATGGCAC 9301 TCCAGTCGCC TTCCCGTTCC GCTATCGGCT GAATTTGATT GCGAGTGAGA TATTTATGCC 9361 AGCCAGCCAG ACGCAGACGC GCCGAGACAG AACTTAATGG GCCCGCTAAC AGCGCGATTT 9421 GCTGGTGACC CAATGCGACC AGATGCTCCA CGCCCAGTCG CGTACCGTCT TCATGGGAGA 9481 AAATAATACT GTTGATGGGT GTCTGGTCAG AGACATCAAG AAATAACGCC GGAACATTAG 9541 TGCAGGCAGC TTCCACAGCA ATGGCATCCT GGTCATCCAG CGGATAGTTA ATGATCAGCC 9601 CACTGACGCG TTGCGCGAGA AGATTGTGCA CCGCCGCTTT ACAGGCTTCG ACGCCGCTTC 9661 GTTCTACCAT CGACACCACC ACGCTGGCAC CCAGTTGATC GGCGCGAGAT TTAATCGCCG 9721 CGACAATTTG CGACGGCGCG TGCAGGGCCA GACTGGAGGT GGCAACGCCA ATCAGCAACG 9781 ACTGTTTGCC CGCCAGTTGT TGTGCCACGC GGTTGGGAAT GTAATTCAGC TCCGCCATCG 9841 CCGCTTCCAC TTTTTCCCGC GTTTTCGCAG AAACGTGGCT GGCCTGGTTC ACCACGCGGG 9901 AAACGGTCTG ATAAGAGACA CCGGCATACT CTGCGACATC GTATAACGTT ACTGGTTTCA 9961 CATTCACCAC CCTGAATTGA CTCTCTTCCG GGCGCTATCA TGCCATACCG CGAAAGGTTT 10021 TGCACCATTC GATGGTGTCA ACGTAAATGC ATGCCGCTCG CCTTCCATGG GTATGGACAG 10081 TTTTCCCTTT GATATGTAAC GGTGAACAGT TGTTCTACTT TTGTTTGTTA GTCTTGATGC 10141 TTCACTGATA GATACAAGAG CCATAAGAAC CTCAGATCCT TCCGTATTTA GCCAGTATGT 10201 TCTCTAGTGT GGTTCGTTGT TTTTGCGTGA GCCATGAGAA CGAACCATTG AGATCATACT 10261 TACTTTGCAT GTCACTCAAA AATTTTGCCT CAAAACTGGT GAGCTGAATT TTTGCAGTTA 10321 AAGCATCGTG TAGTGTTTTT CTTAGTCCGT TATGTAGGTA GGAATCTGAT GTAATGGTTG 10381 TTGGTATTTT GTCACCATTC ATTTTTATCT GGTTGTTCTC AAGTTCGGTT ACGAGATCCA 10441 TTTGTCTATC TAGTTCAACT TGGAAAATCA ACGTATCAGT CGGGCGGCCT CGCTTATCAA 10501 CCACCAATTT CATATTGCTG TAAGTGTTTA AATCTTTACT TATTGGTTTC AAAACCCATT 10561 GGTTAAGCCT TTTAAACTCA TGGTAGTTAT TTTCAAGCAT TAACATGAAC TTAAATTCAT 10621 CAAGGCTAAT CTCTATATTT GCCTTGTGAG TTTTCTTTTG TGTTAGTTCT TTTAATAACC 10681 ACTCATAAAT CCTCATAGAG TATTTGTTTT CAAAAGACTT AACATGTTCC AGATTATATT 10741 TTATGAATTT TTTTAACTGG AAAAGATAAG GCAATATCTC TTCACTAAAA ACTAATTCTA 10801 ATTTTTCGCT TGAGAACTTG GCATAGTTTG TCCACTGGAA AATCTCAAAG CCTTTAACCA 10861 AAGGATTCCT GATTTCCACA GTTCTCGTCA TCAGCTCTCT GGTTGCTTTA GCTAATACAC 10921 CATAAGCATT TTCCCTACTG ATGTTCATCA TCTGAACGTA TTGGTTATAA GTGAACGATA 10981 CCGTCCGTTC TTTCCTTGTA GGGTTTTCAA TCGTGGGGTT GAGTAGTGCC ACACAGCATA 11041 AAATTAGCTT GGTTTCATGC TCCGTTAAGT CATAGCGACT AATCGCTAGT TCATTTGCTT 11101 TGAAAACAAC TAATTCAGAC ATACATCTCA ATTGGTCTAG GTGATTTTAA TCACTATACC 11161 AATTGAGATG GGCTAGTCAA TGATAATTAC TAGTCCTTTT CCTTTGAGTT GTGGGTATCT 11221 GTAAATTCTG CTAGACCTTT GCTGGAAAAC TTGTAAATTC TGCTAGACCC TCTGTAAATT 11281 CCGCTAGACC TTTGTGTGTT TTTTTTGTTT ATATTCAAGT GGTTATAATT TATAGAATAA 11341 AGAAAGAATA AAAAAAGATA AAAAGAATAG ATCCCAGCCC TGTGTATAAC TCACTACTTT 11401 AGTCAGTTCC GCAGTATTAC AAAAGGATGT CGCAAACGCT GTTTGCTCCT CTACAAAACA 11461 GACCTTAAAA CCCTAAAGGC TTAAGTAGCA CCCTCGCAAG CTCGGTTGCG GCCGCAATCG 11521 GGCAAATCGC TGAATATTCC TTTTGTCTCC GACCATCAGG CACCTGAGTC GCTGTCTTTT 11581 TCGTGACATT CAGTTCGCTG CGCTCACGGC TCTGGCAGTG AATGGGGGTA AATGGCACTA 11641 CAGGCGCCTT TTATGGATTC ATGCAAGGAA ACTACCCATA ATACAAGAAA AGCCCGTCAC 11701 GGGCTTCTCA GGGCGTTTTA TGGCGGGTCT GCTATGTGGT GCTATCTGAC TTTTTGCTGT 11761 TCAGCAGTTC CTGCCCTCTG ATTTTCCAGT CTGACCACTT CGGATTATCC CGTGACAGGT 11821 CATTCAGACT GGCTAATGCA CCCAGTAAGG CAGCGGTATC ATCAACGGGG TCTGACGCTC 11881 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 11941 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 12001 CTTGGTCTGA CAGTTACGTT TCCAACCAAT TAACCAATTC TGATTAGAAA AACTCATCGA 12061 GCATCAAATG AAACTGCAAT TTATTCATAT CAGGATTATC AATACCATAT TTTTGAAAAA 12121 GCCGTTTCTG TAATGAAGGA GAAAACTCAC CGAGGCAGTT CCATAGGATG GCAAGATCCT 12181 GGTATCGGTC TGCGATTCCG ACTCGTCCAA CATCAATACA ACCTATTAAT TTCCCCTCGT 12241 CAAAAATAAG GTTATCAAGT GAGAAATCAC CATGAGTGAC GACTGAATCC GGTGAGAATG 12301 GCAAAAGCTT ATGCATTTCT TTCCAGACTT GTTCAACAGG CCAGCCATTA CGCTCGTCAT 12361 CAAAATCACT CGCATCAACC AAACCGTTAT TCATTCGTGA TTGCGCCTGA GCGAGACGAA 12421 ATACGCGATC GCTGTTAAAA GGACAATTAC AAACAGGAAT CGAATGCAAC CGGCGCAGGA 12481 ACACTGCCAG CGCATCAACA ATATTTTCAC CTGAATCAGG ATATTCTTCT AATACCTGGA 12541 ATGCTGTTTT CCCGGGGATC GCAGTGGTGA GTAACCATGC ATCATCAGGA GTACGGATAA 12601 AATGCTTGAT GGTCGGAAGA GGCATAAATT CCGTCAGCCA GTTTAGTCTG ACCATCTCAT 12661 CTGTAACATC ATTGGCAACG CTACCTTTGC CATGTTTCAG AAACAACTCT GGCGCATCGG 12721 GCTTCCCATA CAATCGATAG ATTGTCGCAC CTGATTGCCC GACATTATCG CGAGCCCATT 12781 TATACCCATA TAAATCAGCA TCCATGTTGG AATTTAATCG CGGCCTCGAG CAAGACGTTT 12841 CCCGTTGAAT ATGGCTCATA ACACCCCTTG TATTACTGTT TATGTAAGCA GACAGTTTTA 12901 TTGTTCATGA TGATATATTT TTATCTTGTG CAATGTAACA TCAGAGATTT TGAGACACAA 12961 CGTGGCTTTC CCTGCAGGGT TTGCAGTCAG AGTAGAATAG AAGTATCAAA AAAAGCACCG 13021 ACTCGGTGCC ACTTTTTCAA GTTGATAACG GACTAGCCTT ATTTTAACTT GCTATGCTGT 13081 TTTGAATGGT TCCAACAAGA TTATTTTATA ACTTTTATAA CAAATAATCA AGGAGAAATT 13141 CAAAGAAATT TATCAGCCAT AAAACAATAC TTAATACTAT AGAATGATAA CAAAATAAAC 13201 TACTTTTTAA AAGAATTTTG TGTTATAATC TATTTATTAT TAAGTATTGG GTAATATTTT 13261 TTGAAGAGAT ATTTTGAAAA AGAAAAATT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.