pCas-Amp plasmid

pCas-Amp plasmid

PVT95085

pCas-Amp plasmid Information

Plasmid name: pCas(#62225)-Amp
Alias:
Gene lenght:
Host: E.coli
Use(s): Gene editing
Fragment type: CRISPR
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: J23119
Replicon: pSC101
Growth Strain(s): Stbl3
Growth Temperature: 30°C
Vector backbone:
Forward primer: Amp-F
Reverse primer:
Copy number: Low
Induction: IPTG Induction,Arabinose Induction

pCas-Amp plasmid Description

pCas-Amp is a plasmid suitable for gene editing in Escherichia coli.

pCas-Amp plasmid Sequence

1 AATTTTTCTT TTTCAAAATA TCTCTTCAAA AAATATTACC CAATACTTAA TAATAAATAG 61 ATTATAACAC AAAATTCTTT TAAAAAGTAG TTTATTTTGT TATCATTCTA TAGTATTAAG 121 TATTGTTTTA TGGCTGATAA ATTTCTTTGA ATTTCTCCTT GATTATTTGT TATAAAAGTT 181 ATAAAATAAT CTTGTTGGAA CCATTCAAAA CAGCATAGCA AGTTAAAATA AGGCTAGTCC 241 GTTATCAACT TGAAAAAGTG GCACCGAGTC GGTGCTTTTT TTGATACTTC TATTCTACTC 301 TGACTGCAAA CCCTGCAGGG AAAGCCACGT TGTGTCTCAA AATCTCTGAT GTTACATTGC 361 ACAAGATAAA AATATATCAT CATGAACAAT AAAACTGTCT GCTTACATAA ACAGTAATAC 421 AAGGGGTGTT cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 481 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag 541 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt 601 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt 661 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga 721 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt 781 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga 841 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag 901 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg 961 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg 1021 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc 1081 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg 1141 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc 1201 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg 1261 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac 1321 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact 1381 gattaagcat tggtaaTCAG AATTGGTTAA TTGGTTGGAA ACGTAACTGT CAGACCAAGT 1441 TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT 1501 GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG 1561 AGCGTCAGAC CCCGTTGATG ATACCGCTGC CTTACTGGGT GCATTAGCCA GTCTGAATGA 1621 CCTGTCACGG GATAATCCGA AGTGGTCAGA CTGGAAAATC AGAGGGCAGG AACTGCTGAA 1681 CAGCAAAAAG TCAGATAGCA CCACATAGCA GACCCGCCAT AAAACGCCCT GAGAAGCCCG 1741 TGACGGGCTT TTCTTGTATT ATGGGTAGTT TCCTTGCATG AATCCATAAA AGGCGCCTGT 1801 AGTGCCATTT ACCCCCATTC ACTGCCAGAG CCGTGAGCGC AGCGAACTGA ATGTCACGAA 1861 AAAGACAGCG ACTCAGGTGC CTGATGGTCG GAGACAAAAG GAATATTCAG CGATTTGCCC 1921 GATTGCGGCC GCAACCGAGC TTGCGAGGGT GCTACTTAAG CCTTTAGGGT TTTAAGGTCT 1981 GTTTTGTAGA GGAGCAAACA GCGTTTGCGA CATCCTTTTG TAATACTGCG GAACTGACTA 2041 AAGTAGTGAG TTATACACAG GGCTGGGATC TATTCTTTTT ATCTTTTTTT ATTCTTTCTT 2101 TATTCTATAA ATTATAACCA CTTGAATATA AACAAAAAAA ACACACAAAG GTCTAGCGGA 2161 ATTTACAGAG GGTCTAGCAG AATTTACAAG TTTTCCAGCA AAGGTCTAGC AGAATTTACA 2221 GATACCCACA ACTCAAAGGA AAAGGACTAG TAATTATCAT TGACTAGCCC ATCTCAATTG 2281 GTATAGTGAT TAAAATCACC TAGACCAATT GAGATGTATG TCTGAATTAG TTGTTTTCAA 2341 AGCAAATGAA CTAGCGATTA GTCGCTATGA CTTAACGGAG CATGAAACCA AGCTAATTTT 2401 ATGCTGTGTG GCACTACTCA ACCCCACGAT TGAAAACCCT ACAAGGAAAG AACGGACGGT 2461 ATCGTTCACT TATAACCAAT ACGTTCAGAT GATGAACATC AGTAGGGAAA ATGCTTATGG 2521 TGTATTAGCT AAAGCAACCA GAGAGCTGAT GACGAGAACT GTGGAAATCA GGAATCCTTT 2581 GGTTAAAGGC TTTGAGATTT TCCAGTGGAC AAACTATGCC AAGTTCTCAA GCGAAAAATT 2641 AGAATTAGTT TTTAGTGAAG AGATATTGCC TTATCTTTTC CAGTTAAAAA AATTCATAAA 2701 ATATAATCTG GAACATGTTA AGTCTTTTGA AAACAAATAC TCTATGAGGA TTTATGAGTG 2761 GTTATTAAAA GAACTAACAC AAAAGAAAAC TCACAAGGCA AATATAGAGA TTAGCCTTGA 2821 TGAATTTAAG TTCATGTTAA TGCTTGAAAA TAACTACCAT GAGTTTAAAA GGCTTAACCA 2881 ATGGGTTTTG AAACCAATAA GTAAAGATTT AAACACTTAC AGCAATATGA AATTGGTGGT 2941 TGATAAGCGA GGCCGCCCGA CTGATACGTT GATTTTCCAA GTTGAACTAG ATAGACAAAT 3001 GGATCTCGTA ACCGAACTTG AGAACAACCA GATAAAAATG AATGGTGACA AAATACCAAC 3061 AACCATTACA TCAGATTCCT ACCTACATAA CGGACTAAGA AAAACACTAC ACGATGCTTT 3121 AACTGCAAAA ATTCAGCTCA CCAGTTTTGA GGCAAAATTT TTGAGTGACA TGCAAAGTAA 3181 GTATGATCTC AATGGTTCGT TCTCATGGCT CACGCAAAAA CAACGAACCA CACTAGAGAA 3241 CATACTGGCT AAATACGGAA GGATCTGAGG TTCTTATGGC TCTTGTATCT ATCAGTGAAG 3301 CATCAAGACT AACAAACAAA AGTAGAACAA CTGTTCACCG TTACATATCA AAGGGAAAAC 3361 TGTCCATACC CATGGAAGGC GAGCGGCATG CATTTACGTT GACACCATCG AATGGTGCAA 3421 AACCTTTCGC GGTATGGCAT GATAGCGCCC GGAAGAGAGT CAATTCAGGG TGGTGAATGT 3481 GAAACCAGTA ACGTTATACG ATGTCGCAGA GTATGCCGGT GTCTCTTATC AGACCGTTTC 3541 CCGCGTGGTG AACCAGGCCA GCCACGTTTC TGCGAAAACG CGGGAAAAAG TGGAAGCGGC 3601 GATGGCGGAG CTGAATTACA TTCCCAACCG CGTGGCACAA CAACTGGCGG GCAAACAGTC 3661 GTTGCTGATT GGCGTTGCCA CCTCCAGTCT GGCCCTGCAC GCGCCGTCGC AAATTGTCGC 3721 GGCGATTAAA TCTCGCGCCG ATCAACTGGG TGCCAGCGTG GTGGTGTCGA TGGTAGAACG 3781 AAGCGGCGTC GAAGCCTGTA AAGCGGCGGT GCACAATCTT CTCGCGCAAC GCGTCAGTGG 3841 GCTGATCATT AACTATCCGC TGGATGACCA GGATGCCATT GCTGTGGAAG CTGCCTGCAC 3901 TAATGTTCCG GCGTTATTTC TTGATGTCTC TGACCAGACA CCCATCAACA GTATTATTTT 3961 CTCCCATGAA GACGGTACGC GACTGGGCGT GGAGCATCTG GTCGCATTGG GTCACCAGCA 4021 AATCGCGCTG TTAGCGGGCC CATTAAGTTC TGTCTCGGCG CGTCTGCGTC TGGCTGGCTG 4081 GCATAAATAT CTCACTCGCA ATCAAATTCA GCCGATAGCG GAACGGGAAG GCGACTGGAG 4141 TGCCATGTCC GGTTTTCAAC AAACCATGCA AATGCTGAAT GAGGGCATCG TTCCCACTGC 4201 GATGCTGGTT GCCAACGATC AGATGGCGCT GGGCGCAATG CGCGCCATTA CCGAGTCCGG 4261 GCTGCGCGTT GGTGCGGATA TCTCGGTAGT GGGATACGAC GATACCGAAG ACAGCTCATG 4321 TTATATCCCG CCGTTAACCA CCATCAAACA GGATTTTCGC CTGCTGGGGC AAACCAGCGT 4381 GGACCGCTTG CTGCAACTCT CTCAGGGCCA GGCGGTGAAG GGCAATCAGC TGTTGCCCGT 4441 CTCACTGGTG AAAAGAAAAA CCACCCTGGC GCCCAATACG CAAACCGCCT CTCCCCGCGC 4501 GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC CGACTGGAAA GCGGGCAGTG 4561 AGCGCAACGC AATTAATGTA AGTTAGCGCG AATTGATCTG GTTTGACAGC TTATCATCGA 4621 CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC GGAAGCTGTG GTATGGCTGT 4681 GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC GCACTCCCGT TCTGGATAAT 4741 GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC TGAAATGAGC TGTTGACAAT 4801 TAATCATCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA TAACAATTTC ACACATCTGC 4861 TAATCCTGTT ACCAGGTTTT AGAGCTAGAA ATAGCAAGTT AAAATAAGGC TAGTCCGTTA 4921 TCAACTTGAA AAAGTGGCAC CGAGTCGGTG CTTTTTTTGA GATCTGTCCA TACCCATGGA 4981 TTCTTCGTCT GTTTCTACTG GTATTGGCAC AAACCTGATT CCAATTTGAG CAAGGCTATG 5041 TGCCATCTCG ATACTCGTTC TTAACTCAAC AGAAGATGCT TTGTGCATAC AGCCCCTCGT 5101 TTATTATTTA TCTCCTCAGC CAGCCGCTGT GCTTTCAGTG GATTTCGGAT AACAGAAAGG 5161 CCGGGAAATA CCCAGCCTCG CTTTGTAACG GAGTAGACGA AAGTGATTGC GCCTACCCGG 5221 ATATTATCGT GAGGATGCGT CATCGCCATT GCTCCCCAAA TACAAAACCA ATTTCAGCCA 5281 GTGCCTCGTC CATTTTTTCG ATGAACTCCG GCACGATCTC GTCAAAACTC GCCATGTACT 5341 TTTCATCCCG CTCAATCACG ACATAATGCA GGCCTTCACG CTTCATACGC GGGTCATAGT 5401 TGGCAAAGTA CCAGGCATTT TTTCGCGTCA CCCACATGCT GTACTGCACC TGGGCCATGT 5461 AAGCTGACTT TATGGCCTCG AAACCACCGA GCCGGAACTT CATGAAATCC CGGGAGGTAA 5521 ACGGGCATTT CAGTTCAAGG CCGTTGCCGT CACTGCATAA ACCATCGGGA GAGCAGGCGG 5581 TACGCATACT TTCGTCGCGA TAGATGATCG GGGATTCAGT AACATTCACG CCGGAAGTGA 5641 ATTCAAACAG GGTTCTGGCG TCGTTCTCGT ACTGTTTTCC CCAGGCCAGT GCTTTAGCGT 5701 TAACTTCCGG AGCCACACCG GTGCAAACCT CAGCAAGCAG GGTGTGGAAG TAGGACATTT 5761 TCATGTCAGG CCACTTCTTT CCGGAGCGGG GTTTTGCTAT CACGTTGTGA ACTTCTGAAG 5821 CGGTGATGAC GCCGAGCCGT AATTTGTGCC ACGCATCATC CCCCTGTTCG ACAGCTCTCA 5881 CATCGATCCC GGTACGCTGC AGGATAATGT CCGGTGTCAT GCTGCCACCT TCTGCTCTGC 5941 GGCTTTCTGT TTCAGGAATC CAAGAGCTTT TACTGCTTCG GCCTGTGTCA GTTCTGACGA 6001 TGCACGAATG TCGCGGCGAA ATATCTGGGA ACAGAGCGGC AATAAGTCGT CATCCCATGT 6061 TTTATCCAGG GCGATCAGCA GAGTGTTAAT CTCCTGCATG GTTTCATCGT TAACCGGAGT 6121 GATGTCGCGT TCCGGCTGAC GTTCTGCAGT GTATGCAGTA TTTTCGACAA TGCGCTCGGC 6181 TTCATCCTTG TCATAGATAC CAGCAAATCC GAAGGCCAGA CGGGCACACT GAATCATGGC 6241 TTTATGACGT AACATCCGTT TGGGATGCGA CTGCCACGGC CCCGTGATTT CTCTGCCTTC 6301 GCGAGTTTTG AATGGTTCGC GGCGGCATTC ATCCATCCAT TCGGTAACGC AGATCGGATG 6361 ATTACGGTCC TTGCGGTAAA TCCGGCATGT ACAGGATTCA TTGTCCTGCT CAAAGTCCAT 6421 GCCATCAAAC TGCTGGTTTT CATTGATGAT GCGGGACCAG CCATCAACGC CCACCACCGG 6481 AACGATGCCA TTCTGCTTAT CAGGAAAGGC GTAAATTTCT TTCGTCCACG GATTAAGGCC 6541 GTACTGGTTG GCAACGATCA GTAATGCGAT GAACTGCGCA TCGCTGGCAT CACCTTTAAA 6601 TGCCGTCTGG CGAAGAGTGG TGATCAGTTC CTGTGGGTCG ACAGAATCCA TGCCGACACG 6661 TTCAGCCAGC TTCCCAGCCA GCGTTGCGAG TGCAGTACTC ATTCGTTTTA TACCTCTGAA 6721 TCAATATCAA CCTGGTGGTG AGCAATGGTT TCAACCATGT ACCGGATGTG TTCTGCCATG 6781 CGCTCCTGAA ACTCAACATC GTCATCAAAC GCACGGGTAA TGGATTTTTT GCTGGCCCCG 6841 TGGCGTTGCA AATGATCGAT GCATAGCGAT TCAAACAGGT GCTGGGGCAG GCCTTTTTCC 6901 ATGTCGTCTG CCAGTTCTGC CTCTTTCTCT TCACGGGCGA GCTGCTGGTA GTGACGCGCC 6961 CAGCTCTGAG CCTCAAGACG ATCCTGAATG TAATAAGCGT TCATGGCTGA ACTCCTGAAA 7021 TAGCTGTGAA AATATCGCCC GCGAAATGCC GGGCTGATTA GGAAAACAGG AAAGGGGGTT 7081 AGTGAATGCT TTTGCTTGAT CTCAGTTTCA GTATTAATAT CCATTTTTTA TAACCTCCTT 7141 AGAGCTCGAA TTCCCAAAAA AACGGGTATG GAGAAACAGT AGAGAGTTGC GATAAAAAGC 7201 GTCAGGTAGG ATCCGCTAAT CTTATGGATA AAAATGCTAT GGCATAGCAA AGTGTGACGC 7261 CGTGCAAATA ATCAATGTGG ACTTTTCTGC CGTGATTATA GACACTTTTG TTACGCGTTT 7321 TTGTCATGGC TTTGGTCCCG CTTTGTTACA GAATGCTTTT AATAAGCGGG GTTACCGGTT 7381 TGGTTAGCGA GAAGAGCCAG TAAAAGACGC AGTGACGGCA ATGTCTGATG CAATATGGAC 7441 AATTGGTTTC TTCTCTGAAT GGCGGGAGTA TGAAAAGTAT GGCTGAAGCG CAAAATGATC 7501 CCCTGCTGCC GGGATACTCG TTTAATGCCC ATCTGGTGGC GGGTTTAACG CCGATTGAGG 7561 CCAACGGTTA TCTCGATTTT TTTATCGACC GACCGCTGGG AATGAAAGGT TATATTCTCA 7621 ATCTCACCAT TCGCGGTCAG GGGGTGGTGA AAAATCAGGG ACGAGAATTT GTTTGCCGAC 7681 CGGGTGATAT TTTGCTGTTC CCGCCAGGAG AGATTCATCA CTACGGTCGT CATCCGGAGG 7741 CTCGCGAATG GTATCACCAG TGGGTTTACT TTCGTCCGCG CGCCTACTGG CATGAATGGC 7801 TTAACTGGCC GTCAATATTT GCCAATACGG GGTTCTTTCG CCCGGATGAA GCGCACCAGC 7861 CGCATTTCAG CGACCTGTTT GGGCAAATCA TTAACGCCGG GCAAGGGGAA GGGCGCTATT 7921 CGGAGCTGCT GGCGATAAAT CTGCTTGAGC AATTGTTACT GCGGCACATG GAAGCGATTA 7981 ACGAGTCGCT CCATCCACCG ATGGATAATC GGGTACGCGA GGCTTGTCAG TACATCAGCG 8041 ATCACCTGGC AGACAGCAAT TTTGATATCG CCAGCGTCGC ACAGCATGTT TGCTTGTCGC 8101 CGTCGCGTCT GTCACATCTT TTCCGCCAGC AGTTAGGGAT TAGCGTCTTA AGCTGGCGCG 8161 AGGACCAACG TATCAGCCAG GCGAAGCTGC TTTTGAGCAC CACCCGGATG CCTATCGCCA 8221 CCGTCGGTCG CAATGTTGGT TTTGACGATC AACTCTACTT CTCGCGGGTA TTTAAAAAAT 8281 GCACCGGGGC CAGCCCGAGC GAGTTCCGTG CCGGTTGTGA AGAAAAAGTG AATGATGTAG 8341 CCGTCAAGTT GTCATAATAA ATCGATGCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA 8401 CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG TCTAGATTAA 8461 GAAATAATCT TCATCTAAAA TATACTTCAG TCACCTCCTA GCTGACTCAA ATCAATGCGT 8521 GTTTCATAAA GACCAGTGAT GGATTGATGG ATAAGAGTGG CATCTAAAAC TTCTTTTGTA 8581 GACGTATATC GTTTACGATC AATTGTTGTA TCAAAATATT TAAAAGCAGC GGGAGCTCCA 8641 AGATTCGTCA ACGTAAATAA ATGAATAATA TTTTCTGCTT GTTCACGTAT TGGTTTGTCT 8701 CTATGTTTGT TATATGCACT AAGAACTTTA TCTAAATTGG CATCTGCTAA AATAACACGC 8761 TTAGAAAATT CACTGATTTG CTCAATAATC TCATCTAAAT AATGCTTATG CTGCTCCACA 8821 AACAATTGTT TTTGTTCGTT ATCTTCTGGA CTACCCTTCA ACTTTTCATA ATGACTAGCT 8881 AAATATAAAA AATTCACATA TTTGCTTGGC AGAGCCAGCT CATTTCCTTT TTGTAATTCT 8941 CCGGCACTAG CCAGCATCCG TTTACGACCG TTTTCTAACT CAAAAAGACT ATATTTAGGT 9001 AGTTTAATGA TTAAGTCTTT TTTAACTTCC TTATATCCTT TAGCTTCTAA AAAGTCAATC 9061 GGATTTTTTT CAAAGGAACT TCTTTCCATA ATTGTGATCC CTAGTAACTC TTTAACGGAT 9121 TTTAACTTCT TCGATTTCCC TTTTTCCACC TTAGCAACCA CTAGGACTGA ATAAGCTACC 9181 GTTGGACTAT CAAAACCACC ATATTTTTTT GGATCCCAGT CTTTTTTACG AGCAATAAGC 9241 TTGTCCGAAT TTCTTTTTGG TAAAATTGAC TCCTTGGAGA ATCCGCCTGT CTGTACTTCT 9301 GTTTTCTTGA CAATATTGAC TTGGGGCATG GACAATACTT TGCGCACTGT GGCAAAATCT 9361 CGCCCTTTAT CCCAGACAAT TTCTCCAGTT TCCCCATTAG TTTCGATTAG AGGGCGTTTG 9421 CGAATCTCTC CATTTGCAAG TGTAATTTCT GTTTTGAAGA AGTTCATGAT ATTAGAGTAA 9481 AAGAAATATT TTGCGGTTGC TTTGCCTATT TCTTGCTCAG ACTTAGCAAT CATTTTACGA 9541 ACATCATAAA CTTTATAATC ACCATAGACA AACTCCGATT CAAGTTTTGG ATATTTCTTA 9601 ATCAAAGCAG TTCCAACGAC GGCATTTAGA TACGCATCAT GGGCATGATG GTAATTGTTA 9661 ATCTCACGTA CTTTATAGAA TTGGAAATCT TTTCGGAAGT CAGAAACTAA TTTAGATTTT 9721 AAGGTAATCA CTTTAACCTC TCGAATAAGT TTATCATTTT CATCGTATTT AGTATTCATG 9781 CGACTATCCA AAATTTGTGC CACATGCTTA GTGATTTGGC GAGTTTCAAC CAATTGGCGT 9841 TTGATAAAAC CAGCTTTATC AAGTTCACTC AAACCTCCAC GTTCAGCTTT CGTTAAATTA 9901 TCAAACTTAC GTTGAGTGAT TAACTTGGCG TTTAGAAGTT GTCTCCAATA GTTTTTCATC 9961 TTTTTGACTA CTTCTTCACT TGGAACGTTA TCCGATTTAC CACGATTTTT ATCAGAACGC 10021 GTTAAGACCT TATTGTCTAT TGAATCGTCT TTAAGGAAAC TTTGTGGAAC AATGTGATCG 10081 ACATCATAAT CACTTAAACG ATTAATATCT AATTCTTGGT CCACATACAT GTCTCTTCCA 10141 TTTTGGAGAT AATAGAGATA GAGCTTTTCA TTTTGCAATT GAGTATTTTC AACAGGATGC 10201 TCTTTAAGAA TCTGACTTCC TAATTCTTTG ATACCTTCTT CGATTCGTTT CATACGCTCT 10261 CGCGAATTTT TCTGGCCCTT TTGAGTTGTC TGATTTTCAC GTGCCATTTC AATAACGATA 10321 TTTTCTGGCT TATGCCGCCC CATTACTTTG ACCAATTCAT CAACAACTTT TACAGTCTGT 10381 AAAATACCTT TTTTAATAGC AGGGCTACCA GCTAAATTTG CAATATGTTC ATGTAAACTA 10441 TCGCCTTGTC CAGACACTTG TGCTTTTTGA ATGTCTTCTT TAAATGTCAA ACTATCATCA 10501 TGGATCAGCT GCATAAAATT GCGATTGGCA AAACCATCTG ATTTCAAAAA ATCTAATATT 10561 GTTTTGCCAG ATTGCTTATC CCTAATACCA TTAATCAATT TTCGAGACAA ACGTCCCCAA 10621 CCAGTATAAC GGCGACGTTT AAGCTGTTTC ATCACCTTAT CATCAAAGAG GTGAGCATAT 10681 GTTTTAAGTC TTTCCTCAAT CATCTCCCTA TCTTCAAATA AGGTCAATGT TAAAACAATA 10741 TCCTCTAAGA TATCTTCATT TTCTTCATTA TCCAAAAAAT CTTTATCTTT AATAATTTTT 10801 AGCAAATCAT GGTAGGTACC TAATGAAGCA TTAAATCTAT CTTCAACTCC TGAAATTTCA 10861 ACACTATCAA AACATTCTAT TTTTTTGAAA TAATCTTCTT TTAATTGCTT AACGGTTACT 10921 TTTCGATTTG TTTTGAAGAG TAAATCAACA ATGGCTTTCT TCTGTTCACC TGAAAGAAAT 10981 GCTGGTTTTC GCATTCCTTC AGTAACATAT TTGACCTTTG TCAATTCGTT ATAAACCGTA 11041 AAATACTCAT AAAGCAAACT ATGTTTTGGT AGTACTTTTT CATTTGGAAG ATTTTTATCA 11101 AAGTTTGTCA TGCGTTCAAT AAATGATTGA GCTGAAGCAC CTTTATCGAC AACTTCTTCA 11161 AAATTCCATG GGGTAATTGT TTCTTCAGAC TTCCGAGTCA TCCATGCAAA ACGACTATTG 11221 CCACGCGCCA ATGGACCAAC ATAATAAGGA ATTCGAAAAG TCAAGATTTT TTCAATCTTC 11281 TCACGATTGT CTTTTAAAAA TGGATAAAAG TCTTCTTGTC TTCTCAAAAT AGCATGCAGC 11341 TCACCCAAGT GAATTTGATG GGGAATAGAG CCGTTGTCAA AGGTCCGTTG CTTGCGCAGC 11401 AAATCTTCAC GATTTAGTTT CACCAATAAT TCCTCAGTAC CATCCATTTT TTCTAAAATT 11461 GGTTTGATAA ATTTATAAAA TTCTTCTTGG CTAGCTCCCC CATCAATATA ACCTGCATAT 11521 CCGTTTTTTG ATTGATCAAA AAAGATTTCT TTATACTTTT CTGGAAGTTG TTGTCGAACT 11581 AAAGCTTTTA AAAGAGTCAA GTCTTGATGA TGTTCATCGT AGCGTTTAAT CATTGAAGCT 11641 GATAGGGGAG CCTTAGTTAT TTCAGTATTT ACTCTTAGGA TATCTGAAAG TAAAATAGCA 11701 TCTGATAAAT TCTTAGCTGC CAAAAACAAA TCAGCATATT GATCTCCAAT TTGCGCCAAT 11761 AAATTATCTA AATCATCATC GTAAGTATCT TTTGAAAGCT GTAATTTAGC ATCTTCTGCC 11821 AAATCAAAAT TTGATTTAAA ATTAGGGGTC AAACCCAATG ACAAAGCAAT GAGATTCCCA 11881 AATAAGCCAT TTTTCTTCTC ACCGGGGAGC TGAGCAATGA GATTTTCTAA TCGTCTTGAT 11941 TTACTCAATC GTGCAGAAAG AATCGCTTTA GCATCTACTC CACTTGCGTT AATAGGGTTT 12001 TCTTCAAATA ATTGATTGTA GGTTTGTACC AACTGGATAA ATAGTTTGTC CACATCACTA 12061 TTATCAGGAT TTAAATCTCC CTCAATCAAA AAATGACCAC GAAACTTAAT CATATGCGCT 12121 AAGGCCAAAT AGATTAAGCG CAAATCCGCT TTATCAGTAG AATCTACCAA TTTTTTTCGC 12181 AGATGATAGA TAGTTGGATA TTTCTCATGA TAAGCAACTT CATCTACTAT ATTTCCAAAA 12241 ATAGGATGAC GTTCATGCTT CTTGTCTTCT TCCACCAAAA AAGACTCTTC AAGTCGATGA 12301 AAGAAACTAT CATCTACTTT CGCCATCTCA TTTGAAAAAA TCTCCTGTAG ATAACAAATA 12361 CGATTCTTCC GACGTGTATA CCTTCTACGA GCTGTCCGTT TGAGACGAGT CGCTTCCGCT 12421 GTCTCTCCAC TGTCAAATAA AAGAGCCCCT ATAAGATTTT TTTTGATACT GTGGCGGTCT 12481 GTATTTCCCA GAACCTTGAA CTTTTTAGAC GGAACCTTAT ATTCATCAGT GATCACCGCC 12541 CATCCGACGC TATTTGTGCC GATATCTAAG CCTATTGAGT ATTTCTTATC CATTTTTGCC 12601 TCCTAAAATA AAAAGTTTAA ATTAAATCCA TAATGAGTTT GATGATTTCA ATAATAGTTT 12661 TAATGACCTC CGAAATTAGT TTAATATGCT TT

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.