pLV2-U6-Alyref-shRNA1-EGFP-Pur

pLV2-U6-Alyref-shRNA1-EGFP-Puro plasmid

PVT95359

pLV2-U6-Alyref-shRNA1-EGFP-Puro plasmid Information

Plasmid name: pLV2-U6-Alyref(mouse)-shRNA1-EGFP-Puro
Alias: CCAGCTTGTCACATCACAGAT
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: PGK-R
Copy number: High
Induction:

pLV2-U6-Alyref-shRNA1-EGFP-Puro plasmid Description

pLV2-U6-Alyref-shRNA1-EGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-U6-Alyref-shRNA1-EGFP-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgatctgggc aggaagaggg cctatttccc atgattcctt 2221 catatttgca tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa 2281 acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg 2341 cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt 2401 tcgatttctt ggctttatat atcttgtgga aaggacgagg atccgCCAGC TTGTCACATC 2461 ACAGATttca agagaATCTG TGATGTGACA AGCTGGTTTT TTgaattcta ccgggtaggg 2521 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg 2581 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg 2641 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc 2701 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact 2761 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc 2821 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc 2881 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc 2941 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct 3001 ccgggccttt cgacctgcag cccaagctta ccgccaccat ggtgagcaag ggcgaggagc 3061 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 3121 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 3181 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg 3241 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 3301 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 3361 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 3421 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 3481 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 3541 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 3601 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc 3661 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 3721 ccgggatcac tctcggcatg gacgagctgt acaaggaatt tggcagcggc cagtgcacca 3781 actatgccct gctgaaactg gctggcgatg tggaaagcaa ccccggaccc atgaccgagt 3841 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg 3901 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg 3961 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 4021 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 4081 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 4141 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt 4201 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 4261 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 4321 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 4381 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc 4441 acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcggcg tctcgagtct 4501 agagggcccg acgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga 4561 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatggctt 4621 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 4681 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 4741 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 4801 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 4861 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 4921 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 4981 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 5041 ctctgcggct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg 5101 cctccccgcc tggaattaat tctgcagtcg agacctagaa aaacatggag caatcacaag 5161 tagcaataca gcagctatca atgattgtgc ctggctagaa gcacaagagg aggaggaggt 5221 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga 5281 tcttagccac tttttaaaag aaaagagggg actggaaggg ctaattcact cccaacgaag 5341 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa 5401 ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt 5461 accagttgag ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca 5521 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga 5581 cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg 5641 ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct 5701 gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct 5761 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga 5821 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc 5881 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct 5941 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa 6001 tatcagagag tgagaggcct tgacattgct agcgtttacc gtcgacctct agctagagct 6061 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 6121 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 6181 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 6241 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 6301 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 6361 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 6421 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 6481 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 6541 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 6601 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 6661 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 6721 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 6781 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 6841 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 6901 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 6961 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 7021 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 7081 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 7141 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 7201 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 7261 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 7321 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 7381 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 7441 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 7501 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 7561 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 7621 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 7681 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 7741 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 7801 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 7861 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 7921 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 7981 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 8041 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 8101 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 8161 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 8221 cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa 8281 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 8341 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct 8401 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg 8461 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt 8521 tgttaaatat gtactacaaa cttagtagtt tttaaagaaa ttgtatttgt taaatatgta 8581 ctacaaactt agtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.