pLV3-EF1a-ATP2A2-T2A-Tdtomato

pLV3-EF1a-ATP2A2-T2A-Tdtomato plasmid

PVT90624

pLV3-EF1a-ATP2A2-T2A-Tdtomato plasmid Information

Plasmid name: pLV3-EF1a-ATP2A2(human)-T2A-Tdtomato
Alias: NM_170665.4;ATP2B; DAR; DD; SERCA2
Gene lenght: 3126bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Red
Promoter: EF1a
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: Low
Induction:

pLV3-EF1a-ATP2A2-T2A-Tdtomato plasmid Description

pLV3-EF1a-ATP2A2-T2A-Tdtomato It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-EF1a-ATP2A2-T2A-Tdtomato plasmid Sequence

1 catgtgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg 61 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga 121 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa 181 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg 241 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag 301 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc 361 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg 421 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 481 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 541 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 601 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata 661 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt 721 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca 781 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agcgcgtttt gcctgtactg 841 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac 901 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt 961 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca 1021 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag 1081 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc 1141 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa 1201 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa 1261 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc 1321 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct 1381 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt 1441 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca 1501 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag 1561 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat 1621 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg 1681 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt 1741 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca 1801 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca 1861 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg 1921 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt 1981 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag 2041 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag 2101 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta 2161 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag 2221 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac 2281 cattatcgtt tcagacccac ctcccaaccc cgaggggacc ccgggtttat tacagggaca 2341 gcagagatcc actttggcgc cggctcgagt tttaaaagaa aaggggggat tggggggtac 2401 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa 2461 aaacaaatta caaaaattca aaatttttcg agtggctccg gtgcccgtca gtgggcagag 2521 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc 2581 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 2641 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 2701 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc 2761 tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc tgcagtacgt 2821 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta 2881 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt 2941 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta 3001 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg 3061 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc 3121 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg 3181 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc 3241 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc 3301 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc 3361 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga 3421 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac 3481 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt 3541 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt 3601 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc 3661 atttcaggtg tcgtgagGGA ATTCGCCACC atggagaacg cgcacaccaa gacggtggag 3721 gaggtgctgg gccacttcgg cgtcaacgag agtacggggc tgagcctgga acaggtcaag 3781 aagcttaagg agagatgggg ctccaacgag ttaccggctg aagaaggaaa aaccttgctg 3841 gaacttgtga ttgagcagtt tgaagacttg ctagttagga ttttattact ggcagcatgt 3901 atatcttttg ttttggcttg gtttgaagaa ggtgaagaaa caattacagc ctttgtagaa 3961 ccttttgtaa ttttactcat attagtagcc aatgcaattg tgggtgtatg gcaggaaaga 4021 aatgctgaaa atgccatcga agcccttaag gaatatgagc ctgaaatggg caaagtgtat 4081 cgacaggaca gaaagagtgt gcagcggatt aaagctaaag acatagttcc tggtgatatt 4141 gtagaaattg ctgttggtga caaagttcct gctgatataa ggttaacttc catcaaatct 4201 accacactaa gagttgacca gtcaattctc acaggtgaat ctgtctctgt catcaagcac 4261 actgatcccg tccctgaccc acgagctgtc aaccaagata aaaagaacat gctgttttct 4321 ggtacaaaca ttgctgctgg gaaagctatg ggagtggtgg tagcaactgg agttaacacc 4381 gaaattggca agatccggga tgaaatggtg gcaacagaac aggagagaac accccttcag 4441 caaaaactag atgaatttgg ggaacagctt tccaaagtca tctcccttat ttgcattgca 4501 gtctggatca taaatattgg gcacttcaat gacccggttc atggagggtc ctggatcaga 4561 ggtgctattt actactttaa aattgcagtg gccctggctg tagcagccat tcctgaaggt 4621 ctgcctgcag tcatcaccac ctgcctggct cttggaactc gcagaatggc aaagaaaaat 4681 gccattgttc gaagcctccc gtctgtggaa acccttggtt gtacttctgt tatctgctca 4741 gacaagactg gtacacttac aacaaaccag atgtcagtct gcaggatgtt cattctggac 4801 agagtggaag gtgatacttg ttcccttaat gagtttacca taactggatc aacttatgca 4861 cctattggag aagtgcataa agatgataaa ccagtgaatt gtcaccagta tgatggtctg 4921 gtagaattag caacaatttg tgctctttgt aatgactctg ctttggatta caatgaggca 4981 aagggtgtgt atgaaaaagt tggagaagct acagagactg ctctcacttg cctagtagag 5041 aagatgaatg tatttgatac cgaattgaag ggtctttcta aaatagaacg tgcaaatgcc 5101 tgcaactcag tcattaaaca gctgatgaaa aaggaattca ctctagagtt ttcacgtgac 5161 agaaagtcaa tgtcggttta ctgtacacca aataaaccaa gcaggacatc aatgagcaag 5221 atgtttgtga agggtgctcc tgaaggtgtc attgacaggt gcacccacat tcgagttgga 5281 agtactaagg ttcctatgac ctctggagtc aaacagaaga tcatgtctgt cattcgagag 5341 tggggtagtg gcagcgacac actgcgatgc ctggccctgg ccactcatga caacccactg 5401 agaagagaag aaatgcacct tgaggactct gccaacttta ttaaatatga gaccaatctg 5461 accttcgttg gctgcgtggg catgctggat cctccgagaa tcgaggtggc ctcctccgtg 5521 aagctgtgcc ggcaagcagg catccgggtc atcatgatca ctggggacaa caagggcact 5581 gctgtggcca tctgtcgccg catcggcatc ttcgggcagg atgaggacgt gacgtcaaaa 5641 gctttcacag gccgggagtt tgatgaactc aacccctccg cccagcgaga cgcctgcctg 5701 aacgcccgct gttttgctcg agttgaaccc tcccacaagt ctaaaatcgt agaatttctt 5761 cagtcttttg atgagattac agctatgact ggcgatggcg tgaacgatgc tcctgctctg 5821 aagaaagccg agattggcat tgctatgggc tctggcactg cggtggctaa aaccgcctct 5881 gagatggtcc tggcggatga caacttctcc accattgtgg ctgccgttga ggaggggcgg 5941 gcaatctaca acaacatgaa acagttcatc cgctacctca tctcgtccaa cgtcggggaa 6001 gttgtctgta ttttcctgac agcagccctt ggatttcccg aggctttgat tcctgttcag 6061 ctgctctggg tcaatctggt gacagatggc ctgcctgcca ctgcactggg gttcaaccct 6121 cctgatctgg acatcatgaa taaacctccc cggaacccaa aggaaccatt gatcagcggg 6181 tggctctttt tccgttactt ggctattggc tgttacgtcg gcgctgctac cgtgggtgct 6241 gctgcatggt ggttcattgc tgctgacggt ggtccaagag tgtccttcta ccagctgagt 6301 catttcctac agtgtaaaga ggacaacccg gactttgaag gcgtggattg tgcaatcttt 6361 gaatccccat acccgatgac aatggcgctc tctgttctag taactataga aatgtgtaac 6421 gccctcaaca gcttgtccga aaaccagtcc ttgctgagga tgcccccctg ggagaacatc 6481 tggctcgtgg gctccatctg cctgtccatg tcactccact tcctgatcct ctatgtcgaa 6541 cccttgccac tcatcttcca gatcacaccg ctgaacgtga cccagtggct gatggtgctg 6601 aaaatctcct tgcccgtgat tctcatggat gagacgctca agtttgtggc ccgcaactac 6661 ctggaacctg gtaaagagtg tgtgcagcct gccaccaaat cctgctcgtt ctcggcatgc 6721 accgatggga tttcctggcc gtttgtgctg ctcataatgc ccctggtgat ctgggtctat 6781 agcacagaca ctaactttag cgatatgttc tggtctgagg gcagaggaag tcttctaaca 6841 tgcggtgacg tggaggagaa tcccggccct tccggaacca tggtgagcaa gggcgaggag 6901 gtcatcaaag agttcatgcg cttcaaggtg cgcatggagg gctccatgaa cggccacgag 6961 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg 7021 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttcatg 7081 tacggctcca aggcgtacgt gaagcacccc gccgacatcc ccgattacaa gaagctgtcc 7141 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc 7201 gtgacccagg actcctccct gcaggacggc acgctgatct acaaggtgaa gatgcgcggc 7261 accaacttcc cccccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc 7321 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agatccacca ggccctgaag 7381 ctgaaggacg gcggccacta cctggtggag ttcaagacca tctacatggc caagaagccc 7441 gtgcaactgc ccggctacta ctacgtggac accaagctgg acatcacctc ccacaacgag 7501 gactacacca tcgtggaaca gtacgagcgc tccgagggcc gccaccacct gttcctgggg 7561 catggcaccg gcagcaccgg tagcggcagc tccggcaccg cctcctccga ggacaacaac 7621 atggccgtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc catgaacggc 7681 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc 7741 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag 7801 ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag 7861 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggtctg 7921 gtgaccgtga cccaggactc ctccctgcag gacggcacgc tgatctacaa ggtgaagatg 7981 cgcggcacca acttcccccc cgacggcccc gtaatgcaga agaagaccat gggctgggag 8041 gcctccaccg agcgcctgta cccccgcgac ggcgtgctga agggcgagat ccaccaggcc 8101 ctgaagctga aggacggcgg ccactacctg gtggagttca agaccatcta catggccaag 8161 aagcccgtgc aactgcccgg ctactactac gtggacacca agctggacat cacctcccac 8221 aacgaggact acaccatcgt ggaacagtac gagcgctccg agggccgcca ccacctgttc 8281 ctgtacggca tggacgagct gtacaagtaa ggatccgccc gcaggtacca ccacactgta 8341 caagtaacgc gttaagtcga caatcaacct ctggattaca aaatttgtga aagattgact 8401 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 8461 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 8521 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 8581 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 8641 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 8701 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca 8761 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 8821 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 8881 ctgcggcctc ttccgcgtct ttgccttcgc cctcagacga gtcggatctc cctttgggcc 8941 gcctccccgc gtcgacttta agaccaatga cttacaaggc agctgtagat cttagccact 9001 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga taagatctgc 9061 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct 9121 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt 9181 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt 9241 ggaaaatctc tagcagtacg tatagtagtt catgtcatct tattattcag tatttataac 9301 ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt 9361 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 9421 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc 9481 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg 9541 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca 9601 gaagtagtga ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag 9661 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 9721 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 9781 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg 9841 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca 9901 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc 9961 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct 10021 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg 10081 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc 10141 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg 10201 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg 10261 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg 10321 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 10381 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc 10441 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa 10501 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac 10561 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga 10621 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag 10681 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca 10741 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca 10801 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa 10861 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc 10921 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa 10981 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag 11041 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct 11101 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac 11161 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa 11221 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt 11281 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat 11341 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg 11401 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 11461 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 11521 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 11581 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 11641 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 11701 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 11761 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 11821 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 11881 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 11941 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 12001 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 12061 ttttacggtt cctggccttt tgctggcctt ttgctca

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.