pEF1a-Mito4x-GCaMP6f plasmid

pEF1a-Mito4x-GCaMP6f plasmid

PVT95028

pEF1a-Mito4x-GCaMP6f plasmid Information

Plasmid name: pEF1a-Mito4x-GCaMP6f
Alias:
Gene lenght: 1764bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species:
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7
Reverse primer:
Copy number: High
Induction:

pEF1a-Mito4x-GCaMP6f plasmid Description

pEF1a-Mito4x-GCaMP6f is a plasmid suitable for protein expression in mammalian cells.

pEF1a-Mito4x-GCaMP6f plasmid Sequence

1 AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA 61 TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG 121 TCGACGGATC GGGAGATCTC CCGATCCCCT ATGGTGCACT CTCAGTACAA TCTGCTCTGA 181 TGCCGCATAG TTAAGCCAGT ATCTGCTCCC TGCTTGTGTG TTGGAGGTCG CTGAGTAGTG 241 CGCGAGCAAA ATTTAAGCTA CAACAAGGCA AGGCTTGACC GACAATTGCA TGAAGAATCT 301 GCTTAGGGTT AGGCGTTTTG CGCTGCTTCG CGATGTACGG GCCAGATATA CGCGTTGACA 361 TTGATTATTG ACTAGGCTTT TGCAAAAAGC TTTGCAAAGA TGGATAAAGT TTTAAACAGA 421 GAGGAATCTT TGCAGCTAAT GGACCTTCTA GGTCTTGAAA GGAGTGGGAA TTGGCTCCGG 481 TGCCCGTCAG TGGGCAGAGC GCACATCGCC CACAGTCCCC GAGAAGTTGG GGGGAGGGGT 541 CGGCAATTGA ACCGGTGCCT AGAGAAGGTG GCGCGGGGTA AACTGGGAAA GTGATGTCGT 601 GTACTGGCTC CGCCTTTTTC CCGAGGGTGG GGGAGAACCG TATATAAGTG CAGTAGTCGC 661 CGTGAACGTT CTTTTTCGCA ACGGGTTTGC CGCCAGAACA CAGGTAAGTG CCGTGTGTGG 721 TTCCCGCGGG CCTGGCCTCT TTACGGGTTA TGGCCCTTGC GTGCCTTGAA TTACTTCCAC 781 CTGGCTGCAG TACGTGATTC TTGATCCCGA GCTTCGGGTT GGAAGTGGGT GGGAGAGTTC 841 GAGGCCTTGC GCTTAAGGAG CCCCTTCGCC TCGTGCTTGA GTTGAGGCCT GGCCTGGGCG 901 CTGGGGCCGC CGCGTGCGAA TCTGGTGGCA CCTTCGCGCC TGTCTCGCTG CTTTCGATAA 961 GTCTCTAGCC ATTTAAAATT TTTGATGACC TGCTGCGACG CTTTTTTTCT GGCAAGATAG 1021 TCTTGTAAAT GCGGGCCAAG ATCTGCACAC TGGTATTTCG GTTTTTGGGG CCGCGGGCGG 1081 CGACGGGGCC CGTGCGTCCC AGCGCACATG TTCGGCGAGG CGGGGCCTGC GAGCGCGGCC 1141 ACCGAGAATC GGACGGGGGT AGTCTCAAGC TGGCCGGCCT GCTCTGGTGC CTGGCCTCGC 1201 GCCGCCGTGT ATCGCCCCGC CCTGGGCGGC AAGGCTGGCC CGGTCGGCAC CAGTTGCGTG 1261 AGCGGAAAGA TGGCCGCTTC CCGGCCCTGC TGCAGGGAGC TCAAAATGGA GGACGCGGCG 1321 CTCGGGAGAG CGGGCGGGTG AGTCACCCAC ACAAAGGAAA AGGGCCTTTC CGTCCTCAGC 1381 CGTCGCTTCA TGTGACTCCA CGGAGTACCG GGCGCCGTCC AGGCACCTCG ATTAGTTCTC 1441 GAGCTTTTGG AGTACGTCGT CTTTAGGTTG GGGGGAGGGG TTTTATGCGA TGGAGTTTCC 1501 CCACACTGAG TGGGTGGAGA CTGAAGTTAG GCCAGCTTGG CACTTGATGT AATTCTCCTT 1561 GGAATTTGCC CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA 1621 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTAGC TTGGTACTAA TACGACTCAC 1681 TATAGGGAGA CCCAAGCTGG CTAGGTAAGC TTGGTACCGA GCTCGGATCC ACTAGTCCAG 1741 TGTGGTGGAA TTCTGCAGAT ATCCAGCACA GTGGCGGCCG CgccaccATG AGCGTGCTGA 1801 CACCTCTGCT GCTGAGAGGC CTGACAGGCT CTGCTAGAAG GCTGCCTGTG CCTAGAGCCA 1861 AGATCCACTC TCTGGGCGAC CCTATGTCTG TGCTGACTCC ACTGCTCCTG CGGGGACTGA 1921 CTGGATCTGC TCGTAGACTG CCAGTGCCAC GGGCTAAGAT TCACAGCCTG GGCGATCCAA 1981 TGAGTGTGCT TACCCCACTC CTGCTCAGAG GACTGACCGG AAGTGCTAGA CGCCTGCCAG 2041 TTCCAAGGGC CAAAATCCAT AGTCTGGGAG ATCCCATGTC CGTGTTGACA CCCCTCTTGC 2101 TGAGGGGACT TACCGGCTCA GCTCGGAGAC TCCCTGTTCC AAGAGCAAAG ATTCATTCCC 2161 TCGGCGACCC AAGATCTGGC AGCGCTAAAG ACCCTACCAT GGGTAGCCAC CACCACCATC 2221 ACCACGGCAT GGCTTCTATG ACAGGCGGCC AGCAGATGGG CCGCGACCTG TACGATGACG 2281 ACGATAAGGA CCTGGCCACA ATGGTGGACA GCAGCAGGCG GAAGTGGAAC AAGACAGGCC 2341 ACGCCGTTAG AGCCATCGGC AGACTGTCTA GCCTGGAAAA CGTGTACATC AAGGCCGACA 2401 AGCAGAAGAA CGGCATCAAG GCTAACTTCA AGATCCGGCA CAACATCGAG GACGGCGGAG 2461 TGCAGCTGGC TTACCACTAC CAGCAGAACA CCCCTATCGG CGACGGACCA GTTCTGCTGC 2521 CTGACAACCA CTACCTGAGC GTGCAGAGCA AGCTGAGCAA GGACCCCAAC GAGAAGAGGG 2581 ACCACATGGT GCTGCTGGAA TTTGTGACCG CCGCTGGCAT CACACTCGGC ATGGACGAAC 2641 TGTACAAAGG CGGCACAGGC GGCTCTATGG TGTCCAAGGG CGAAGAACTG TTCACCGGCG 2701 TGGTGCCCAT TCTGGTGGAA CTGGATGGGG ATGTGAACGG CCACAAGTTC AGCGTCAGCG 2761 GAGAAGGCGA AGGCGACGCC ACATACGGAA AGCTGACCCT GAAGTTCATC TGCACCACCG 2821 GCAAACTGCC CGTGCCTTGG CCTACACTGG TCACCACACT GACATACGGC GTGCAGTGCT 2881 TCAGCAGATA CCCCGACCAT ATGAAGCAGC ACGACTTCTT CAAGAGCGCC ATGCCTGAGG 2941 GCTACATCCA AGAGAGAACC ATCTTCTTTA AGGACGACGG CAACTACAAG ACCAGGGCCG 3001 AAGTGAAGTT CGAGGGCGAC ACCCTGGTCA ACAGAATCGA GCTGAAGGGC ATCGACTTCA 3061 AAGAGGACGG CAACATCCTG GGCCACAAGC TCGAGTACAA CCTGCCTGAC CAGCTGACCG 3121 AGGAACAGAT CGCCGAGTTC AAAGAAGAGT TCAGCCTGTT CGACAAGGAC GGCGACGGCA 3181 CAATCACCAC CAAAGAACTG GGCACCGTGA TGAGAAGCCT GGGACAGAAC CCTACAGAGG 3241 CCGAGCTGCA GGACATGATC AACGAGGTTG ACGCTGATGG CGACGGAACT ATCGACTTCC 3301 CCGAGTTCCT GACCATGATG GCCAGAAAGA TGAAGTACAG GGACACAGAG GAAGAGATCC 3361 GCGAGGCCTT CGGCGTGTTC GATAAGGATG GAAACGGCTA CATCTCTGCC GCTGAGCTGA 3421 GACACGTGAT GACCAACCTG GGCGAGAAGC TGACAGACGA AGAGGTGGAC GAGATGATCA 3481 GAGAGGCCGA CATTGACGGC GACGGGCAAG TGAACTACGA AGAGTTTGTC CAGATGATGA 3541 CCGCCAAGTG AGTTTAAACC CGCTGATCAG CCTCGACTGT GCCTTCTAGT TGCCAGCCAT 3601 CTGTTGTTTG CCCCTCCCCC GTGCCTTCCT TGACCCTGGA AGGTGCCACT CCCACTGTCC 3661 TTTCCTAATA AAATGAGGAA ATTGCATCGC ATTGTCTGAG TAGGTGTCAT TCTATTCTGG 3721 GGGGTGGGGT GGGGCAGGAC AGCAAGGGGG AGGATTGGGA AGACAATAGC AGGCATGCTG 3781 GGGATGCGGT GGGCTCTATG GCTTCTGAGG CGGAAAGAAC CAGCTGGGGC TCTAGGGGGT 3841 ATCCCCACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG 3901 TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC 3961 TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC 4021 GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA 4081 GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA 4141 ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG 4201 ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA 4261 AATTTAACGC GAATTAATTC TGTGGAATGT GTGTCAGTTA GGGTGTGGAA AGTCCCCAGG 4321 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCAGGTGTGG 4381 AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG TATGCAAAGC ATGCATCTCA ATTAGTCAGC 4441 AACCATAGTC CCGCCCCTAA CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA 4501 TTCTCCGCCC CATGGCTGAC TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCTGC 4561 CTCTGAGCTA TTCCAGAAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA 4621 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT 4681 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT 4741 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT 4801 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA 4861 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC 4921 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG 4981 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC 5041 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA 5101 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA 5161 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC 5221 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA 5281 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA 5341 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG 5401 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT 5461 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTCGCGA AATGACCGAC CAAGCGACGC 5521 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG 5581 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT 5641 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA 5701 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC 5761 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT 5821 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC 5881 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA 5941 TTGCGTTGCG CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT 6001 GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC 6061 TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG 6121 CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG 6181 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC 6241 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA 6301 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT 6361 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC 6421 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC 6481 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA 6541 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT 6601 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA 6661 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT 6721 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT 6781 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT 6841 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC 6901 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA 6961 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA 7021 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC 7081 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT 7141 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA 7201 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT 7261 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT 7321 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG 7381 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC 7441 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC 7501 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG 7561 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA 7621 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA 7681 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT 7741 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG 7801 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.